Name: Kent Orville F.

Dolor Date: December 10, 2019

Adventist Medical Center - Manila June-December 2019 Batch

A Post-Seminar Report on
Beyond Routine: Advances in Medical Laboratory Science

FERDINAND E. MENDOZA: GUT MICROBIOME TESTING

The gut microbiome is a complex community of microorganisms living inside our

digestive tract. This can affect our overall health. The testing determines which kind of organisms present
and also the correct balance between these microorganisms. When you have the right symbiotic
relationship with your body’s bacteria, you can optimize your well-being. Well-balanced bacteria levels
allow you to modulate your immunity, absorb foods more effectively, and combat disease.

NORIEL A. CASTRO: QUALITY ASSURANCE PROGRAM IN THE CLINICAL

LABORATORY

Quality assurance refers to establishing and maintaining a certain level of quality in a

product or service. Therefore, quality assurance in a medical lab refers to making sure the lab is running
smoothly, safely, and effectively without any errors. In the laboratory, quality assurance involves the
entire testing process: pre-analytical, analytical, and post-analytical processes. Pre-analytical: All patient
information and documentation is correct, patient information is kept private, proper procedures are
followed when taking the sample. Analytical: Standard operating procedures are maintained during the
testing of the sample; patient privacy is maintained. Post-analytical: Samples are analyzed using the
correct equipment, equipment is routinely calibrated, patient privacy is maintained. We need to apply this
in everything that we do in the lab in order to produce reliable results to provide the best for our patients.

MARESCIELE G. MENDOZA: POLYMERASE CHAIN REACTION: PRICIPLE AND

APPLICATIONS

PCR uses the enzyme DNA polymerase that directs the synthesis of DNA from
deoxynucleotide substrates on a single-stranded DNA template. DNA polymerase adds nucleotides to the
3` end of a custom-designed oligonucleotide when it is annealed to a longer template DNA. Thus, if a
synthetic oligonucleotide is annealed to a single-stranded template that contains a region complementary
to the oligonucleotide, DNA polymerase can use the oligonucleotide as a primer and elongate its 3` end to
generate an extended region of double stranded DNA. There are three basic steps in PCR. First
Denaturation, The DNA template is heated to 94° C. This breaks the weak hydrogen bonds that hold
DNA strands together in a helix, allowing the strands to separate creating single stranded DNA.
Second, Annealing, the mixture is cooled to anywhere from 50-70° C. This allows the primers to bind to
their complementary sequence in the template DNA. Lastly, Extension, the reaction is then heated to 72°
C, the optimal temperature for DNA polymerase to act. DNA polymerase extends the primers, adding
nucleotides onto the primer in a sequential manner, using the target DNA as a template. As a medical
technologist someday, this can help us in anyways such as analyzing clinical specimens for the presence
of infectious agents, including HIV, hepatitis, malaria and many more. The results can also help on the
patients prognosis.