PROTOCOL

Extended Spectrum Beta Lactmass (ESBL) klebsiella

pneumonia

&

Institutional guide
Dr.Anbumani, MD.,PhD..,
Assistant professor
Department of Medical Microbiology,
Sri Ramachandra Institute of Higher Education & Research
(Deemed university)
Porur, Chennai

Submitted by
S.Abdul hameed
M.sc Medical Laboratory Technology,
Sri Ramachandra Institute of Higher Education & Research
(Deemed university)
Porur, Chennai

Sri Ramachandra Institute of Higher Education & Research

(Deemed university)

INSTITUTIONAL ETHICS COMMITEE

INITIAL REVIEW FOR SUBMISSION FORM FOR RESEARCH PROPOSAL
 Initial Review Submission Form

1. Title of the proposal EXTENDED SPECTRUM BETA LACTAMASS

(ESBL) Klebsiella pneumonia

2. Name of the Investigator S.ABDUL HAMEED,

M.sc Medical laboratory technology,
Department Allied Health Sciences,
Sri Ramachandra Institute of Higher Education &
Research
(Deemed university)
Porur, Chennai
3. Name of the Guide 1. Clinical guide
Dr. Anbumani MD.,PhD..,
Professor
Department of Medical Microbiology
Sri Ramachandra Institute of Higher Education&
Research
(Deemed university)
Porur, Chennai
4. Name of the Co-Guide

5. Name of the HOD Dr.Anupma jyoti kindo.,MD

6. Forwarding letter from the Guide Enclosed

and H.O.D
7. Consent from the Department, if Enclosed
applicable
8. Mention ethical issues to be
addressed in your study

9. Protocol Enclosed in the proposal

10. Consent form Enclosed in Appendix 1

11. Covering letter from the guide Enclosed

with the checklist attached
12. All relevant enclosures like Enclosed in Appendix 2
preformat, case report forms,
questionnaires, follow up cards
etc.
13. CV of the PI and guide Enclosed in Appendix 3
14. Checklist of the guide and Enclosed
students while forwarding the
application to the IEC
 PROTOCOL
TITLE:
EXTENDED SPECTRUM BETA LACTAMASS (ESBL) Klebsiella pneumonia
INTRODUCTION:
Klebsiella species are infection by multidrug –resistant grame negative bacilli that
produce extended spetrum β lactamase (ESBL) enzyme producing organisms.(Itokazu et
al.,1996) β lactam antimicrobial most common treatment for bacterial
ESBL producing organism are inhibited by β lactam β lactam inhibitors but are not
with extende spctrum cephalosporing (Brgess et al.,2001)
Dectection of ESBL enzyme using routione laboratory susceptibility test is often
difficult and consequently, ESBL producing Klebsiella pneumonia may falsely appear to be
susceptible to newer cephalosporins (Gibb et al., 2000)
Klebsiella pneumonia as resistant to all penicilline and cephalosporins including
cefepime and aztreonam irrespective of their individual in vitro test result (NCCLS.,2000) the
present study was designed to determine the antibiotic susceptibility patterns of ESBL
producing K.pneumoniae from different clinical specime.

LITERATURE REVIEW:
1. E Amadi et al 2007 did a study on the antibiotic sensitivity pattern of extended spectrum
beta lactamase (ESBL) producing Klebiella pneumonia isolated from urine and blood
samples was investigated. Sensitivity studies were carried out using disc diffusion
method by Kirby-Bauer and phenotypic characterizaton of ESBL was carried out using
double disc synergy test (DDST). The result of the study revealed that 38 isolates were
positive for ESBL production 22 isolates were from urine, and 16 from blood
respectively. This stdy therefore, do not only proclaim the presence of ESBL producing
K.pneumoniae in abakaliki, nigeria but also emphasizes that they are multi-drug resistant

2. Chen-Hsiang Lee et al 2006 did a study on Klebiella pnumoniae are common human
pathogen that cause a wide spectrum of infections.to better understand the clinical
outcome of patients with extended spectrum β lactamase producing Klebiella pneumonia
ESBL-KP, bacterameia treated with either flomoxef or a carbapenem, in vitro activities
of these antibiotics against ESBL-KP. In vitro activities of flomoxef and carbapenem
against flomoxef-susceptible ESBL-KP isolates were evaluated by susceptibility testing
and time-kill study.the MICs of flomoxef and carbapenem indicated tat the tested ESBL-
 KP were susceptible to these antibiotics regardless of the inoculum size of 105 cfu/ml.
time kill study showed that these antibiotics each acted actively against and inhibited the
regrowth of the tested ESBL-KP for at least 24 h.

3. Vemula Sarojamma et al 2011, did a study on ESBL continue to be a major challenge in

clinical setups world over conferring resistance to the expanded-spectrum
cephalosporins. An attempt was made to study the prevalence of ESBL-producing
Klebsiella pneumoniae clinical isolates in a tertiary care hospital in Kurnool. A total of
hundred collected isolates of Klebsiella pneumoniae was studied for their susceptibility
patterns to various antibiotics and detection of ESBL producers by double disc synergy
test (DDST) and phenotypic confirmatory disc diffusion test (PCDDT). Of the 100
isolates tested for their antibiogram, 61% isolates have shown susceptibility to 3rd-
generation cepholosporins and 39% were resistant. Amoxycillin showed the highest by
DDST.
and PCDDT ESBL producers were more in hospital isolates (28%). Compared to
community isolates (6%) Maximum percentage of ESBL producers were noticed from
blood sample with 57.14%. In the present study, a large number of isolates were found to
be multidrug resistant and ESBL producers. PCDDT was found to be better than DDST
in the detection of ESBLs. Continued monitoring of drug resistance is necessary in
clinical settings for proper disease management.

AIM:
To isolate Extended spectrum beta lactamass (ESBL) Klebsiella pneumonia from clinical
sample.
OBJECTIVE:
Extended Spectrum β Lactamase producing Klebiella pneumonia ESBL-KP, bacterameia
treated with either flomoxef or a carbapenem, in vitro activities of these antibiotics
against ESBL-KP.
METHODOLOGY:
 Site of the study : Centerl laboratory of Sri Ramachandra high education
and research
 Types of study : Prospective
 Period of the study : March 1-March 31
 Sample size :
  Inclusion criteria : Urine, Blood, Sample

METHOD:
ANTIMICROBIAL SUSCEPTIBILITY TESTING:
A total of one months clinical isolates of Klebsiella pneumoniae were isolated from
two clinical specimen namely, urine, blood obtained from Medical Microbiology unit of
Centerl laboratory of Sri Ramachandra high education
The sensitivity studies was conducted to using the KirbyBauer disc diffusion method
various antimicrobial discs were used which include antimicrobials for screening of ESBL
Klebsiella species: sensitivity determination Sterile petri dishes of Mueller Hinton Ager
were prepared according to specification. 0.1 ml of Klebsiella pneumoniae equivalent to 0.5
McFarland standard. The petri dishes containing Mueller Hinton Agar. These were allowed
to stand for 45 minutes to enable the inoculated organisms to pre-diffusion. The following
antibiotics discs were aseptically placed on the surfaces of the sensitivity agar plates;
Ceporex (15µg), Ceftazidime (30µg), Cefotaxime (30µg), ceftriaxone (30µg). These were
incubated for 18 to 24 hrs at 37˚C and the radial zone of inhibition were taken. Organism that
resistant to any of the 2nd and 3rd generation cephalosporins was tested for ESBL production.
DETECTION OF ESBL PRODUCTION:
The isolates that was resistant to any of the 2nd and 3rd generation cephalosporins
(ceftazidime, ceftriaxone cefotaxime) were tested for ESBL production using the double disc
synergy test (DDST). Several plates of MuellerHinton agar were prepared and 30µg discs of
ceftazidime and cefotaxime were placed 15 mm center to center. Inoculated media were
incubated for 18 to 24 hours at 37˚C. Enhanced zone of inhibition between any of the beta-
lactam discs and the center disc was recorded
PLAN ANALYSIS:
ESBL Klebsiella pneumonia

Peptone water incubated at 37˚C for 15 minute

Mueller Hinton agar

 Antibiotic discs are applied with sterile Ceporex (15µg), Ceftazidime (30µg), Cefotaxime
(30µg), ceftriaxone (30µg)

Incubated at 37˚C for 24 hours

Result
REFERENCE:
1. Itokazu G, Quinn J, Bell-Dixon C, Kahan F, Weinstein R. Antimicrobial resistance
rates among aerobic Gram-negative bacilli recovered from patients in intensive care
units: Evaluation of a national postmarketing surveillance program. Clin Infect Dis
1996;23:779–84.
2. D. M. Livermore, “Bacterial resistance: origins, epidemiology, and impact,” Clinical
Infectious Diseases, vol. 36, supplement 1, pp. S11–S23, 2003.
3. Burgess, D.S. Comparison of in - vitro of piperacillin / tazobactam, cefepime,
imipenem and meropenem against extended spectrum - lactamas (ESBL) and non -
ESBL producing K. pneumonia by time kill methodology (abstr). Pharmacotherapy ,
2001, 21: 1273.
4. Gibb, A.P. and Crichton, M. Cefpodoxime screening of Escherichia coli and
Klebsiella spp. by Vitex for detection of organism producing extended - spectrum -
Lactamas. Diag. Microbiol. Infect. Dis., 2000, 38: 255 257.
5. National Committee for Clinical Laboratory Standards. Methods for dilution:
antimicrobial susceptibility test for bacteria that grow aerobically: approved standard,
5th ed. NCCLS document A7 - A5. Wayne, PA; National committee for Clinical
Laboratory Standards, 2000.
6. Peterson, J.E., Harchin, T.C., Kelly, C.A., Harcia, R.C. and Jorgensen, J.H.
Association of antibiotic utilization measures and control of multiple - drug resistance
in Klebsiella pneumoniae. Infect Control Hosp. Epidemiol, 2000, 21:455-8.
7. Wong - Beringer, A. Therapeutic challenges associated with extended - spectrum -
lactamase producing Escherichia coli and Klebsiella pneumonia Pharmacotherapy,
2001, 21:583-92.
8. Nathisuwen, S. Burgess, D.S. and Lewis, J.S. Extended - spectrum - lactamases:
epidemiology, detection and treatment. Pharmacotherapy , 2001, 21: 920 8.
9. Patterson, D.L. Extended - spectrum lactamases: The European experience. Curr.
Opin. Infect. Dis., 2001, 14: 697 – 701.

Checklist for the guides of students while forwarding the

application to the IEC

1. Protocol is complete Yes/No

2. Objectives clearly defined Yes/NO

3. Methodology is described in detail Yes/NO

4. The proposed methods will answer the research question Yes/No

5. Statistical method for sample size estimation/analysis spelt Yes/No

out
6. Informed consent Yes/No
Patient information sheet and informed consent forms are
attached (English and regional language)

7. If children <18 years consent from parent/guardian Yes/No

(Assent form from children 12-18 years)

8. Forwarding letter from the HOD and the guide Yes/No

9. Consent letter from other departments, if applicable Yes/No

10. Ethical issues addressed Yes/No

11. Relevant proforma/case record form attached Yes/No

12. Brief CV of the PI/Guide attached Yes/No

13. Brief CV of the Co-PI if applicable attached
 Signature of the guide with date

February 2019
PLACE ; Chennai
From
Abdul hameed S,
2ndyear M.Sc Medical laboratory technology,
Department of allied health science,
Sri Ramachandra Institute of Higher Education&
Research,
Porur, Chennail-116.

To
The Director of Central laboratory,
Sri Ramachandra Medical College and Research Institute,
Porur, Chennai – 116.

Respected Madam,
Through: Guide Professor Dr.Anbumani department of Microbiology, SRMC&RI
Sub ; Submission of project proposal for M.SC Medical laboratory technology Dissertation,
Reg

I am a second year M.Sc Medical laboratory technology student from the Department
of Microbiology, I kindly request you to grant me permission to conduct my study titled
“Extended spectrum beta lactamass (ESBL) Klebsiella pneumonia”.

Thanking you
Yours Sincerely,
Abdul hameed S

Covering Letter with checklist

February 2019
PLACE –chennai

From
Abdul hameed S
2ndyear M.Sc Medical laboratory technology,
Sri Ramachandra Medical College and Research Institute,
Porur,
Chennail-116

To
The Institutional Ethics Committee,
Sri Ramachandra Medical College and Research Institute,
Porur,
Chennai-116.

Through: Head of the Department, Department of Microbiology. SRMC & RI

Guide Professor Dr.Anbumani, Department of Microbiology, SRMC&RI

Sub : Submission of project proposal for M.Sc Medical laboratory technology Dissertation,
Reg

I am second year M.Sc Medical laboratory technology student from the Department
of Microbiology. I kindly request you to grant me ethical clearance for my study titled
“Extended spectrum beta lactamass (ESBL) Klebsiella pneumonia”. I am herewith
submitting my research proposal.

Thanking you,
Yours Sincerely,
Abdul hameed S

Forwarding Letter

February 2019
PLACE : Chennai
From
Abdul hameed S,
2ndyear M.SC Medical laboratory technology,
Department of Microbiology,
Sri Ramachandra Medical College and Research Institute,
Porur,
Chennai-600116.

To
The Head of the Department,
Department of Microbiology,
Sri Ramachandra Medical College and Research Institute,
Porur,
Chennai – 600116.

Respected Madam

Through, Guide Professor Dr.Anbumani department of Microbiology, SRMC&RI

Sub; Submission of project proposal for M.SC Medical laboratory technology Dissertation,
Reg

I am a second year M.Sc Medical laboratory technology student from the Department
of Microbiology, I kindly request you to grant me permission to conduct my study titled
“Extended spectrum beta lactamass (ESBL) Klebsiella pneumonia”

Thanking you,

Yours Sincerely,
Abdul hameed S

INVESTIGATOR CURRICULUM VITAE

PERSONAL DETAILS
Name : ABDUL HAMEED S
Date of birth : 06/07/1996
Father’s name : Samsu deen A
Mother’s name : Rashitha begam S
Sex : Male
Marital status : Single
Permanent address : 29/246 anantha giri 5th street,
Kodaikanal- 624101, Dindigul,

Mobile no : 7200101612
Email id : abdul98007hameed@gmail.com
Education
EXAMINATION BOARD/ NAME OF YEAR OF
PASSED UNIVERSITY SCHOOL/COLLEGE PASSING
Master in medical Sri ramachandra Sri ramachandra Expected
laboratory technology university medical college & to be
research institute passed out
in june
2019
B.sc microbiology Periyar university AVS college of arts and 2017
science
HSC State board St xaviers higher 2014
secondary school
SSLC State board R.C town higher 2012
secondary school

Declaration

I hereby declare that the information furnished above is true of my best of my knowledge. I
expect your favourable consideration.

Signature
Abdul hameed S

CURRICULUM VITAE Dr N Anbumani MBBS MD (Microbiology) PhD

Professor & Consultant in Microbiology Sri Ramachandra Medical College and
Hospital, Chennai -116

PERSONAL DETAILS & EDUCATION

Name : NarayanaswamyAnbumani

Address : Harmony - 12, 37th street, Nanganallur,Chennai-600 061

Telephone : 044-24765512 ext 231, 9841319069

Email : dranbumani@gmail.com

DOB : 06.12.1973

Citizenship : Indian

Graduation : MBBS (Madras, indian) 1996

Medical council : Tamil nadu medical council 59211

Postgraduate : MD Microbiology in april 2002

Doctorate : PhD in Bacteriology-Enterococcus

 PREVIOUS APPOINTMENTS

1996 - 1997 : Pre-registration House Officer, Madras Medical College, India

1998 - 1999 : Medical Officer, Sri BM Hospital, Chennai -600 061

1999 - 2002 : Postgraduate Training in Microbiology, Dr.AL Mudaliyar Postgraduate

Iinstitute of Basic Medical Sciences, Taramani-600 113

2002 – 2008 : Assistant Professor in Microbiology, Sri Ramachandra Medical

College and Hospital, Porur, Chennai-600 116

2008 – 2012 : Assistant Professor in Microbiology, Sri Ramachandra Medical

College and Hospital, Porur, Chennai-600 116

2012 – to date Professor in Microbiology, Sri Ramachandra Medical College and

Hospital, Porur, Chennai-600 116

PRESENT APPOINTMENTS:

1. Professor in Department of Microbiolgy, Sri Ramachandra Medical College &

Hospita-involves Teaching Postgraduates and guiding them in their
dissertation work.

2. Consultant Microbiologist at Sri Ramachandra Medical Centre.

3. Member of Institutional Ethics Committee for postgraduate students.

Member of Infection Control Committee

PUBLICATIONS:
1. EMM types of streptococcus pyogenes in Chennai T Menon, AM Whatmore,
S Srivani, MP Kumar, N Anbumani, S Rajaji Indian Journal of Medical
Microbiology, 2001 :19: 3: 161-162.

2. Biotypes of GP A streptococci isolated from children .M. Palani Kumar,

Thangam Menon, Charmaine Lobo, N. Anbumani,C. P. Girish Kumar and S.
Shanmugasundaram Journal of Medical Microbiology – 53 (2004) 229-230.

3. Rapid discrimination between strains of β haemolytic strepto cocci by intact

cell mass spectrometry, M. Palani Kumar, M. Vairamani, N. Prasada Raju,
Charmaine Lobo, N. Anbumani, C.P. Girish Kumar, Thangam Menon & S.
Shanmugasundaram Indian Journal of Medical Research 119, June 2004, pp
283-288.

4. Isolation, distribution and prevalence of various species of enterococci

isolated from clinical specimens in a tertiary care hospital. Anbumani.N,
 Menon.T, J.Kalyani, M.Mallika ;Indian Journal of Pathology & Microbiology:
2005, 48(4):534-7.

5. Biotyping of Group A Streptococci isolated from normal school children in

South India. Anbumani N,Thangam Menon Indian Journal for the Practising
Doctors, 2:1:2005

6. A Short-Term Study of Diarrhoea Among Children Under Five Years of Age

in Chennai, Tamilnadu, With Special Reference to Rotavirus,P.K.Rajesh,
M.Kalyani, Anbumani.N , M.Mallika Indian Journal of Practising Doctors,
2:3:2005.

7. Prevalence of Methicillin resistant Staphylococcus aureus in a tertiary care

hospital in Chennai in South India- N.Anbumani, J.Kalyani, M.Mallika. Indian
Journal of Practising Doctors 3:4:2006.

8. Helico bacter pylori antigen detection in stool. Jude Martin, Anbumani.N,

M.Kalyani, P.K.Rajesh Indian Journal of Medical Microbiology.2006
;24(1):79-80.

9. Epidemiology and Microbiology of wound infections. Anbumani N, Kalyani

J, Mallika M.Indian Journal for th Practising Doctors, 3: 5 :2006.

10. Antibiotic resistance pattern in uropathogens in a tertiary care hospital.

Anbumani. N, Mallika M. Indian Journal for the Practising Doctors, 4: 1 2007.

11. Antimicrobial resistance in community and nosocomial Escherichia coli

urinary tract isolates from Chennai, South India in 2007 Narayanaswamy
Anbumani, Varadharajan Mallika; Int. J. Med. Public health 2011;1(1):21-
24.

12. Distribution and Antimicrobial Susceptibility of bacteria isolated from blood

cultures of hospitalized patients in a tertiary care hospital. Anbumani.N,
KalyaniJ, Mallika.M, Indain Journal of Practising Doctor 5:.2 2008.

13. Extended Spectrum beta lactamases in clinical isolates of Klebsiella

pneumoniae in a tertiary care hospital , Chennai, South India.(original article)
N.Anbumani, M,Mallika, Biomedicine 2010, 30 (1) : 25-29.

14. Erythromycin inducible resistance in clinical strains of Staphylococcus species

E.Shamsadh Begum., N.Anbumani, M.Mallika Biomedicine, 2010; 30 (1)
94-96.

15. Speciation and antimicrobial susceptibility pattern of Enterococci from a

tertiary health care center of south India. Anbumani Narayanaswamy ,
Rajalakshmi K, Mallika Varadharajan. Journal of Pharmacy Research
2011,4(4),989-990.
 16. Prevalence and Distribution of Soil Transmitted Helminths (STH) among
Asymptomatic School Going Children in South Chennai, Tamil Nadu, India.
Anbumani. N., Mallika M. Int. J. Med. Public Health 2011;1(2):57-59.

17. Prevalence of high level aminoglycoside resistance among clinical isolates of

Enterococci in a tertiary care hospital, in Chennai - south India Anbumani.N,
Menon.T., J.Kalyani, M.Mallika Biomedicine:2011;31(3):334-340.

18. Antimicrobial resistance pattern and biofilm formation in coagulase-negative

Staphylococcus. Shamsadh Begum E, Anbumani.N., Kalyani.J.,
Biomedicine:2011;31(3):322-328.

19. Antimicrobial resistance and genetic diversity among Enterococcal isolates

from a tertiary care hospital in Chennai, south India. Anbumani.N., Anand
Manoharan., AnilKumar.V,ThangamMenon.,Kalyani.J.,Mallika.M.
Biomedicine: 2012 ; 32(1) : 72-75.

20. Prevalence and antimicrobial susceptibility pattern of Coagulase-negative

Staphylococcus.E. Shamsadh Begum, N. Anbumani, J. Kalyani, M. Mallika.
International Journal of Medicine and Public Health, Vol. 1, Issue 4, Oct-Dec,
2011, 59-62.

21. Survey for virulence associated genes among clinical Enterococcus faecalis
strains isolated in Chennai, South India. Anbumani.N., Thangam Menon .,
Kalyani.J., Mallika.M. Biomedicine:2012; 32(2):179-184.

22. Occurrence, species distribution and antimicrobial resistance of Tribe Proteeae

isolates.E. Shamsadh Begum, N. Anbumani. Indian Journal of Applied
Microbiology 15:1:83-88.

23. Phenotypic detection methods of Carbapenemase production in

Enterobacteriaceae Pandurangan, Sathya, Shamsadh BegumEsak, and
Anbumani Narayanasamy Int. J. Curr. Microbiol. App. Sci 4.6 (2015): 547-
552.

PUBLICATIONS:
 Poster presentation at XXVII National Congress of Indian Association of
Medical Microbiolgists (IAMM –poster no PB 7) at THE TULIP STAR
HOTEL, Juhu Tara Road, Juhu, Mumbai –400059, November 5-9, 2003
on the isolation,characterization and antibiotic susceptibility pattern in
tertiary care hospita.

 Poster Presentation at International Congress Of Immunology, Montreal

[proxy] On “IMMUNOLOGY IN THE DIAGNOSIS OF FEVER” JULY
2004.

 Isolation, distribution and prevalence of various species of Enterococci

from clinical specimens in a tertiary care hospital. XX1X National Congress
 of Indian Association of Medical Microbiolgists, SRMC &RI (DU), Porur,
Chennai-600116.19th October ,23,2005.

 Oral presentation of Antimicrobial susceptibility pattern of clinical isolates

of Enterococci at the National Symposium on Recent trends in
Streptococcal Diseases. 25th February, 2006 (held at BeverlyHotel,
17,Rajarathinam Road Kilpauk, Chennai - 10. )conducted by Dr.Thangam
Menon ,Organizing Secretary the Department of Microbiology, Dr. ALM
Post Graduate Institute of Basic Medical Sciences, University of Madras.

 Oral presentation of Phenotypic and genotypic characterization of High

level Gentamicin resistant Enterococcal isolates in a tertiary care hospital
at SOZOCON, conducted at Sri Manakula Vinayaga Medical
College,Puducherry on 19th January 2008.

 Oral presentation of Phenotypic and genotypic characterization of High

level Gentamicin resistant Enterococcal isolates in a tertiary care hospital
at XXXII National Congress of Indian Association of Medical
Microbiolgists, conducted at armed Forces Medical College,Pune on 23rd
October 2008.

 Anbumani.N, Thangam Menon, Kalyani.J,Mallika.M Oral Presentation

Of Genetic Diversity & Antimicrobial Resistance Among Enterococcal
Isolates From A Tertiary Care Hospital In Chennai, South India. At
APCON -2008, organised by Indian Association of Pathologists &
Microbiologists at SRM, Kattankulathur ,Chennai on December 16th
2008.

 Anbumani.N, Thangam Menon, Kalyani.J,Mallika.M Oral Presentation

Of Genetic Diversity & Antimicrobial Resistance Among Enterococcal
Isolates From A Tertiary Care Hospital In Chennai, South India. At
MICROCON -2009, organised by Indian Association of Medical
Microbiologists at JSS Medical College, Mysore ,on November 6th 2009.

MEMBER
1. MEMBER OF INSTITUTION ETHICS COMMITTEE FOR
POSTGRADUATE STUDIES [IEC].

2. MEMBER OF INFECTION CONTROL COMMITTEE [ICC].

3. MEMBER OF INDIAN ASSOCIATION OF MEDICAL

MICROBIOLOGIST [IAMM].

4. MEMBER OF INDIAN ASSOCIATION OF APPLIED MEDICAL

MICROBIOLOGIST [IAAM].

5. MEMBER OF ACADEMY OF CLINICAL MICROBIOLOGIST

[ACM].
 6. MEMBER OF INTERNATIONAL LEPTOSPIROSIS SOCIETY
[ILS].

ACADEMICS: Undergraduate Teaching

M.B.B.S., B.D.S..,B.Sc..,(Nursing), B.P.T.,B.Sc.,(Optometry) B.Sc.,(Sports
Medicine) B.Sc.,(Allied Health Sciences)
Postgraduate Teaching
M.D., M.D.S., M.Sc.,(Medical Microbilogy,M.L.T.,Bioteachnology,
Clinical Nutrition, Renal Sciences & Neuro Sciences)
DIAGNOSTICS:
Routine Procedures of identifying pathogenic
Organisms from various samples viz.., feces,
Blood, C.S.F, Pus, Urine, respiratory.
Special Serology Testing – Detection of Antibodies for
DsDNA by ELISA & Immunofluorescence,
Detection of antibodies for Dengue, CMV, HSV
RESEARCH:
ICMR FUNDED “Multi Center on Antimicrobial Resistance, Monitoring of Salmonella
Typhi and salmonella paratyphi A-An attempt to make national guideline on
Antibiotic policy to treat Enteric fever”

 Resistance pattern among Enterobacteriaceace especially carbapenemase

resistance pattern among Tribe Proteeae.

 Mupirocin resistance among Staphylococcus isolates (MSSA.MRSA &

CoNS)

 Invitro susceptibility to Colistin and Tigecycline in Carbapenemase producing

Enterobacteriaceae.