PUC BIOLOGY PRACTICAL EXAMINATION QUESTION PAPER & ANSWER KEY

QUESTION PAPER

TIME:2 HOURS MAXIMUM MARKS:30

1.Prepare a temporary slide to show pollen germination form the given material “A” and calculate the percentage of
pollen germination.

Or

Prepare a temporary slide to show pollen tube growth on the stigma from the given material “A” and draw a labeled
diagram of your observation.

Or

Prepare a temporary slide of given material “A” by taking a transverse section of ovary and report the number of
locules and type of Lacentation.

Or

Prepare a temporary slide to show nuclear staining from the given material “A” and report the shape of the cell and
number of nuclei.

2.Prepare a temporary slide of the given material “B” and identify any one stage of mitosis and draw a labeled
diagram of the stage observed.

3.Conduct a Suitable test for the given soil/water samples “C1” and “C2” and report the PH Values.

4.Identify “D” by giving two reasons.

5.Identify the given specimen “E” and name the disease it causes.

6.Identify “F” and comment on it.

7.Viva Voce

8.Practical Records.

ANSWER KEY

1 * POLLEN GERMINATION

Total No.Of Pollen Grains(N) No.of Pollen grains germinated % of pollen germination: nx100/N

% of pollen germination =

 Pollen tube Growth on the stigma

 T.S of Ovary
No. of Locules in the ovary:5(pentalocular)
Type of placentation: Axile Placentation
 Nuclear Staining
Shape of the cell:Rectangular
No. of Nuclei per cell: One(Uninucleate)

2.(Note: In the answer sheet,draw any one stage such as prophase,Metaphase anaphase or telophase)

3.pH of sample C1 and C2

Samples Colour with universal H Result:Acidic/Basic/Neutral
Indicator solution.
C1

C2

4.V.S of ovule showing female gametophyte

Characters:
1. V.S of ovule shows a massive diplcid tissue called nucellus
2. Nucellus is surrounded by integuments.
3. Female gametophyte or embryo sac is located inside the nucellus.
4. Mature embryo sac is a 7-called 8-nucleated structure.

 T.S of mammalian testis

Characters:
1. Mammalian testis consists of about 250 compartments called testicular lobules.
2. Seminiferous tubule is lined with germinal epithelium.
3. Germinal epithelium contains spermatogonia and large cells called Sertoli cells.
4. The connective tissue present between the seminiferrous tubules contain LeyDig cells.

 T.S of mammalian ovary.

Characters:

1.Ovary is solid structure lined by a layer of germinal epithelium.

 2.The cortex of the ovary contains many ovarian follicles of different stages.

3.Mature ovarian follicles are called Graafian Follicles.

4.Graafian Follicle contains a secondary oocyte and a large cavity called antrum.

 V.S of mammalian blastula.

Characters:
1. Mammalian Blastuta is also called blastocyst.
2. The Covering of blastuta is called trophoblast.
3. Trophoblast encloses an inner cellmass and a cavity called Blastocoel.
4. The inner cellmass is Suspendend into the cavity like a Honeycomb.

5.Identification :Entamoeba histolitica

Disease: Amoeboic dysentery or amoebiasis.
 Identification:Plasmodium vivax
Disease: Malaria
 Identification:Ascaris lumbricoides.
Disease: Ascariasis
 Identification: Trichophyton rubrum
Disease: Ringworm.

6.Identification:Hydrohpyte(Eichornia)

1. Eichornia is a free floating hydrophyte.

2. It contains spongy petioles.

3. It contains balancing roots with root pockets.

4. Root,stem and leaves contains aerenchyma.

Identification:Xerophyte(Nerium)

1. Nerium is a Xerophyte.

2. Leave are covered by thick cuticle.

3. Leave contain sunken stomata

4. Xylem is well developed.

Identification:Hydric animal(Fish)

1. Fish is hydric animal.

2. It has stream-lined body.

3. Body is covered by scale.

4. It has fins such as pectoral,pelvis and caudal fins which help in swimming and balancing.
Identification:Xeric animal(Camel)

1. Camel is a xeric animal

2. It has a hump which stores fat.

3. body is covered by thick fur.

4. Its nostrils can be closed.