Beruflich Dokumente
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8: ELECTROPHORESIS
1. Given: Electrophoretic buffer pH: 8.6, protein A: isoelectric point: 7.2, protein B: isoelectric point: 5.1, protein C:
isoelectric point: 8.6. The order of mobility of proteins A, B and C toward the positive electrode (slowest to fastest)
will be:
a. A, B, C
b. B, C, A
c. C, A, B
d. B, A, C
e. C, B, A
Examine the tracing and use the following key to answer questions 2-5:
KEY:
a. alpha 1 globulin
b. beta globulin
c. gamma globulin
d. albumin
e. alpha 2 globulin
2. The area depicted by #1 is _____
3. The area depicted by #2 is _____
4. The area depicted by #3 is _____
5. The area depicted by #4 is _____
6. The electrophoretic pattern above represents a:
a. normal pattern
b. monoclonal peak
c. polyclonal peak
d. relative hypoalbuminemia
e. b and d
Use the following Key to answer questions 7-9:
a. 1, 2 and 3 are correct
b. 1 and 3 are correct
c. 2 and 4 are correct
d. only 4 is correct
e. all are correct
7. Below is a strip demonstrating serum protein electrophoretic bands. Which of the following is (are) true?
Answer:
1. c (p. 203-204,208)
2. c (p. 213-214)
3. b (p. 213)
4. e (p. 213)
5. a (p. 213)
6. a (p. 213, 214)
7. b (p. 204-208)
8. b (p. 207, 211)
9. a (p. 206, 207)
10. c (p. 209-210)
11. b (p. 209-210)