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“The Right to Information, The Right to Live” “Step Out From the Old to the New”

IS 2263 (1979): Methods of Preparation of Indicator

Solutions [CHD 1: Inorganic Chemicals]

“!ान $ एक न' भारत का +नम-ण”

Satyanarayan Gangaram Pitroda
“Invent a New India Using Knowledge”

“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता ह”

है”
ह
Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”
 )
IS:2263-1979
( Reaffirmed 1998 )
Indian Standard
METHODS OF
PREPARATION OF INDICATOR SOLUTIONS

( First Revision )

Second Reprint AUGUST 1991

.iJDc 54-43:54i24

@I Copyright 1980

BUREAU OFINDIAN STANDARDS

MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG
NEW DELHI llOOfY2

Gr 3 January 1980
 IS :2263-1979

Indian Standard
METHODS OF
PREPARATION OF INDICATOR SOLUTIONS

( First Revision )
Chemical Standards Sectional Cr,mmitteo, CDC I

Chairman Rcpresenling
DR H. L. BAMI Central Forensic Scirncc l,;lhr>ratory, Ncw’Delhi

Mrmbrrs
DR K. NARAYANASWAMY ( AIlcrnotc to
Dr H. L. Bami )
AQRICULTURAL M A R K R T I N o Central Agmark Laboratory, Nageur
ADVXSZR
SHRI T. V. MATHI~W ( Alternate )
SHRI A. K. BHATTACHARYA National Test House, Calcutta
SHRI K. C. SEAL ( Allcrnolc )
SERI B. N. BHATTACHAHYYA Gcolngical Survey of Tndia, Calcutta
SHRI V. M. BHUCHAR National Physical Laboratory ( CSIR ), New Delhi
SHRI D. S. ,CHADHA Directorate General of Health Services, New Delhi
SMT DRBI MUKHERJEE ( Altcrnore )
Dk M. S. CHADHA Bhabha Atomic Research Centrc, Bombay
SHRI R. S. CHATIM Municipal Corporation of Greater Bombay
CEEM~ST & M~TALLWW~ST, Railway Board ! Ministry of Railways )
Sou TH CENTKAL RAILWAY,
SBCU~~~ERABAD
ASSISTANT DIRECTOR ( MET ),
RDSO, LUCKNOW ( Alternate )
SBRI D. R. GUPTA Central Revenues Control Laboratory, New Delhi
SHR~ KZSHAV PRASAD ( Afltrnate )
SERB C. HINQA~H The Century Spg & Mfg Co Ltd, 8ombay
SHKI OM PRASAD ( Alrernorr )
DR B. N. MATTOO Maharashtra State Forensic Science Laboratory,
Bombay
DR M. S. MADIWALA ( ANernate )
DR P. R. PABRAI Central Indian Pharmacopoeia Laboratory,
Ghaziabad
SERI J. K. PATWA Sarabhai M. Chemicals, Vadodara
SHRI D. RAMAMURTEY Bharat HFavy Electricals Ltd, Tiruchchirappalli
SERI M. B. UNNI ( Alkrnalc )
( Continued on Pogr 2 )

‘@ Copyright 1980
BUREAU OF INDIAN STANDARPS
This publication is protected under the Indian Copyrighl Ad ( XIV of 1957 ) and
reproduction in whole or in part by any means except with written permission of the
publisher shall be deemed to be an infringement of copyright under the said Act.
IS : 2263 - 1979

( Continuedfrom page 1 )
Members Repfcsenting
SHRI G. K. RAO Ministry of Dcfence ( DGI )
DR A. K. SIN ( Alternate )
DR G. RAMANA RAO Indian Drugs & Pharmaceuticals Ltd, New Delhi
SHRI RANQASWAMY ( Alternate )
DR
_ M. P. SARAKARI Italab Pvt Ltd, Bombay
S&r S. S. NONAVAR ( Alternate )
DR B. R. SANT Regional Research Laboratory ( CSIR ),
Bhubaneshwar
DR T. P. PRASAD ( Altertzats )
SARI H. M. SHAIKH National Organic Chemical Industries Ltd, Bombay
DR V. SIZINIVASAN Glaxo Laboratories ( India ) Ltd, Bombay
DR S. V. VORA ( Alternate )
DR R. T. TJXAMPY Shriram Institute for Industrial Research, Delhi
DR G. M. >AXPNA. Director General, IS1 ( Ex-o$icio Member)
Director ( Chem’)

Secretary

SARI N. K. SHARMA
Deputy Director ( Chem ), IS1

2
 IS : 2263 - 197F

Indian Standard
METHODS OF
PREPARATION OF INDICATOR SOLUTIONS

( First Revision )
0. FOREWORD
0.1 This Indian Standard ( First Revision ) was adopted by the Indian
Standards Institution on 4 May 1979, after the draft finalized by the
Chemical Standards Sectional Committee had been approved by the
Chemical Division Council.

0.2 In testing laboratories, much avoidable duplication is encountered in

nreparing and maintaining more than one solution of the same indicator
:3r the same type of titration when testing according to Indian
Standards. The Sectional Committee responsible for the preparation of
this standard felt that it would be desirable to have a standard prescrib-
ing the preparation of the common indicator solutions. This standard is
intended to serve as a ready guide for the preparation of indicator
solutions.
0.3 This standard was first published in 196’2. This revision incorpo-
rates additional indicators which are now used in chemical analysis.

0.4 In the preparation of’this revision, assistance has been derived from
BS 4123 : 1967 Schedule of preferred chemical indicators. British
Standards-Institution.

1. SCOPE
1.1 This standard prescribes the methods for preparing indicator SO~U-
tions used in volumetric analysis.

2. QUALITY OF REAGENTS
2.1 Unless specified otherwise, pure chemicals and distilled water ( SCG
IS : 1070-1977’ ) shall beemployed in tests.
NOTE - ‘ Pure chemicals ’ shall mean chemicals that do not contain impurities
which affect the results of analysis.
*Specification for water for general laboratory use ( second rcoizion ).

3
 IS : 2263 - 1979

2.2 In the preparat+n of solutions, rectified spirit ( see IS : 323-1959* )

and sodium hydroxide ( see IS : 376-19767 ) shall be used wherever
specified.

3. PREPARATION OF INDICATOR SOLUTIONS

SECTION I pH INDICATORS

3.1 Bromocresol Green - Chemically described as 3, 5, 3’, 5’-tetra-

bromo-n-cresolsulphonphthalein, changes from yellow to blue through~H
range 3.6 to 5.2.
3.1.1 Bromocresol Green Indicator So&on -0.4 g/l:Warm 0.1 g of bromo-
cresol green with 2.9 ml of 0’05 N sodium hydroxide solution and 5 ml of
rectified spirit; after solution is effected, add 50 ml of rectified spirit and
dilute to 250 ml with water.

3.2 Bromocrcsol Purple - Chemically described, as 5, 5’-dibromo-

o-cresolsulphonphthalein, changes from yellow to bluish-violet through
pH range 5.2 to 6.8.

3.2.1 Bromocresol Purple Indicator “Solution - 0.4 g/l. Warm 0.1 g of

bromocresol purple with 5 ml of rectified spirit until dissolved, add
20 ml of rectified spirit and 3.7 ml of 0.05 N sodium hydroxide sol&on.
Mix, add.30 ml of rectified spirit and dilute to 250 ml with water.

3.3 Bromophenol Blue - Chemictilly described as 3, 5, 3’, 5’-tetra-

bromophenolsulphonphthalein, changes from yellow to bluish-violet
through ~JH range 2’8 to 4.6.

3.3.1 Bromophenol Blue Indicator Solution - 0.4 g/l. Warm 0.1 g of

bromophenol blue with 3’0 ml of 0 05 N sodium hydroxide solution and
5 ml of rectified spirit; after solution is effected, add 50 ml of rectified
spirit and dilute to 250 ml with water.

3.4 Bromothymol Blue - Chemically described as 3, 3’-dibromo-

.thvmolsulphonphthalein, changes from yellow to blue through pH
range 6.0 to 7.6.

3.4.1 Bromothymol Blue Indicator Solution - 0.4 g/l. Warm 0.1 g of

bromothymol blue with 3.2 ml of 0.05 N sodium hydroxide solution ind
5 ml of rectified spirit; after solution is effected, add 50 ml of rectified
spirit and dilute to 250 ml with water.
+Sprcification for rectified spirit ( r&red ).
tSpecification for sodium hydroxide, analytical reagent ( second revision).

4
 IS : 2263 - 1979

3.5 m-Cresol Parple - Chemically described as m-cresolsulphon-

phthalein, changes from red to yellow through fiH range 1.2 to 2.8 and
from yellow to violet throughPH range 7.6 to 9.2.
3.5.1 m-Crcsol Purple Indicator Solution - O-4 g/l. Dissolve 0.1 g of
m-cresol purple in 50 ml of rectified spirit, add 5.2 ml of 0’05 N sodium
hydroxide solution and dilute to 250 ml with water.
3.6 Cresol Red - Chemically described as o-cresolsulphonphthalein,
changes from red to yellow through pH range 0.2 to 1.8’and from yellow
to violet-red through PH range 7.2 to 8.8.
3.6.1 Cresol Red Indicator Solution - 0.2 g/l. Warm 50 mg of cresol red
with 2.65 ml of 0’05 N sodium hydroxide solution and 5 ml of rectified
spirit; after solution is effected, add 50ml of rectified spirit and dilute
to 250 ml with water.

3.7 Dimethyl Yellow-Chemically described as 4-dimethylaminoazo-

benzene, changes from red to yellow through PH range 2.8 to 4’6.
3.7.1 Dimethyl Yellow Indicator Solution - 2 g/l. Dissolve 0.5 g OF
dimethyl yellow in 250 ml of rectified spirit.
3.8 2+Dinitrophenol- Changes from colourless to yellow through
PH range 2’8 to 4’4.
3.8.1 2,4-Dinitrophenol Indicator Solution - 1 g/l. Dissolve 0.25 g of
2,4-dinitrophenol in 250 ml of water.

3.9 Methyl Orange - Chemically described as sodium 4’-dimethyl-

aminoazobenzkne-kulphonate, changes from red to yellow through
pH range 2.9 to 4’6.
3.9.1 Methyl Orange Indicator Solution - 0.4 g/l. Dissolve 0.1 g of
methyl orange in 50 ml of rectified spirit and dilute to 250 ml with
water.
3.9.2 Screened Methyl Orange Indicator Solution - 1 g/l. Dissolve 0.27 g
of methyl orange and 0.15 g of xylene cyan01 FF in 50 *ml of rectified
spirit and dilute to 220 ml with water. Changes from red to green
through PH range 2’9 to 4 6.
3.10 Methyl Red - Chemically described as 4’-dimethylaminoazoben-
zene-2-carboxylic acid, changes from red to yellow through pH range 4’2
to 6.3.
3.10.1 Methyl Red Indicator Solution - 0’1 g/l. Warm 25 mg of methyl
red ( finely powdtired ) with 0.95 ml of 0.05 N sodium hydroxide solution
and 5 ml of rectified spirit; after solution is effected,, add 125 ml of
rectified spirit and dilute to 250 ml with water.

5
IS : 2263 - 1979

3.10.2 Screened Methyl Red Indicator Solution - 1 g/l. Dissolve 0.25 g of

water-soluble methyl red and 0.35 g of xylene cyan01 FF in 50 ml of
rectified spirit and dilute to 250 ml with water. Changes from purple to
green through PH range 4.2 to 6.3.

3.10.3 Methyl Red-M~thylene Blue Mixed Indicator Solution - Dissolve

0.5 g of water-soluble methyl red in 250 ml of rectified spirit.
Dissolve 025 g of methylene blue in 250 ml of rectified spirit. Store the
two solutions separately and mix equal volumes as required for use.

Changes from purple to green through PH range 4.2 to 6.3.

3.11 1-Naphtholphthalein - Changes from pale red to blue through
pH range 7.3 to 8.7.

3.11.1 I-JVaphtholphthalein Indicator Solution - 5 g/l. Dissolve 1.25 g of

1-naphtholphthalein in 250 ml of rectified spirit.

3.12 3-Nitrophenol- Changes from colourless to yellow through PH

range 6.8 to 8.4.

3.12.1 3-Ntrophenol Irtdicator Solution - 1 g/l. Dissolve 0’25 g of

3-nitrophenol in 250 ml of water.
3.13 4-Nitrophenol- Changes from colourless to yellow through PH
range 5.0 to 7.0.

3.13.1 4-Ntrophenol Indicator Solution - 1 g/l. Dissolve 0.25 g of

4-nitrophehol in 250 ml of water.

3.14 Fhenolphthalein - Changes from colourless to red through pH

range 8.3 to, 10’0.

3.14.1 Phbnolphthalein Indicator Solution - 10 g/l. Dissolve 2.5 g of

phenolphthzrlein in 250 ml of rectified spirit.

3.15 Phenol Red - Chemically described as phenolsulphonphthalein,

changes from yellow to red through PH range 6.8 to 8.4.

3.15.1 Phenol Red Indicator Solution - 0.2 g/l. Warm 50 mg of phenol

red with 2’85 ml of 0.05 N sodium hydroxide solution and 5 ml of
rectified spirit; after solution is effected, add 50 ml of rectified spirit and
dilute to 250 ml with water.

3.16 Thyme1 Blue - Chemically described as thymolsulphonphthalein,

changes from red to yellow throughpH range 1.2 to 2’8 and from‘yellow
to violet-blue through pH range 8.0 to 9.6.

3.16.1 Thymol Blue Indicator Solution - 0.4 g/l. Warm 0.1 g of thymol
blue with 4’3 ml of 0.05 N sodium hydroxide solution and 5 ml of recti-
fied spirit; after solution is effected, add 50 ml of rectified spirit and
dilute to 250 ml with water.
_Y
6
 IS L 2263 - 1979

3.17 Thymolphthalein - Changes from colourless to blue through PH

range 8.3 to 10.5.

3.17.1 Thymolphthalein Indicator Solution - 2 g/l. Dissolve 0.5 g of

thymolphthalein in 60 ml of rectified spirit and dilute to 250, ml with
water.

SECTION 2 METAL INDICATORS

3.18 Calcein - Chemically described as bis [ NN-di ( carboxymethyl )

aminomethyl] fluorescein, gives a green fluorescence and an orange-yellow
colour. with calcium ions in alkaline solution. When these ions are
absent the solution is not fluorescent and the colour is yellowish red.

3.18.1 Calcein Indicator Mixture - Triturate 1 g of calcein with 100 g of

potassium chloride to a fine powder.

3.19 Calcon - Chemically described as sodium I-( 2-hydroxy-l-

naphthylazo )-2-naphthyi-4-sulphonate, gives a purple-red colour with
calcium ions in alkaline solution. When these ions are absent, the
solution is hlue.

3.19.1 Calcon Indicator Solution - 1 g/l. Dissolve 0.25 g in 250 ml of

water.

3.20 Gatechd Violet - Chemically described as 3, 3’, 4”-tiihydro-

xyfuchsone-2” sulphonic acid, gives a blue colour with bismuth and red
with thorium ions in acid solution. When these ions are absent, the
solution is yellow.

3.20.1 Catechol Violet Indicator Solution - 1 g/l. Dissolve 0’25 g of

qatechol violet in 250 ml of water.

3.21 Dithizone - Chemically described as 1, 5-diphenylthiocarbazone,

gives a pink colour with zinc ions at PH 4 to 5. When these ions are
absent, the solution is green.

3.21.1 Dithizone 1tzdicator Solution - 0.25 g/l. Dissolve 62’5 mg of

dithizone in 250 ml of rectified spirit.

3.22 GbHA - Chemically described as glyoxal bis ( 2-hydroxyanil ),

gives pure yellow colour with calcium ions in alkaline solution.

7
IS r 2263 - 1979

3.22.1 GbHA Indicator Solution - 2’5 g/l. ,Place approximately 0.25 g

of GbHA in a loo-ml daik glass bottle with ground glass stopper. Add
100 ml of rectified spirit and stir until completeljy dissolved.
NOTE - When this indicator is not readily available, it may be prepared as
follows:

Place 8’8 g of chemically pure o-aminophenol in a 3 OOO-ml comcal flask contain-

ing 2 000 ml of water at 80°C. After dissolution, add 8 g of a 300 g per
litrc water solution of glyoxal. Keep the mixture at 80°C for 30 minutes, then
cool to room temperature under running water. Filter the precipitate so obtained
through a Buchner funnel and wash three times. carefully drying after each wash.
Transfer the precipitate to a 250-ml beaker. Add 10 ml of acetone. After
dissolution, add approximately 3 g of activated carbon, stir for one minute, then
filter on a dry Auted filter paper and collect the filtrate in a 750-ml conical flask.
Add.300 ml of water to the filtrate and filter the precipitate through a Buchncr
funnel employing suction. Dry the residue thoroughly, in an oven at 70%. Crusb
and store in a dark air-tight container.

3.23 HSN - Chemically described as 2-hydroxy-1-( 2-hydroxy-4-sulpho-

1-naphthylazo )-3-naphthoic acid, gives a pink colour with calcium ions in
alkaline solution. When these ions are absent, the solution is blue.
3.23.1 HSN Indicator Mixture - Triturate 1 g of HSN with 100 g of
sodium sulphate, anhydrous to a fine powder.
3.24 Methylthymol Blue - Chemically described as 3, 3’-bis [ NN’-di
( carboxymethyl ) aminomethyl ] thymolsulph&phthalein, gives a blue
colour with calcium and -magnesium ions in alkaline solution. When
these ions are absent, the solution is grey.
3.24.1 Methylthymol Blue Indicator Mixture - Triturate 1 g of methyl-
thymol blue’ with 100 g of potassium nitrate to a fine powder.

3.25 Mordant Black 11 -Chemically described as sodium I -( l-hydroxy-

2-naphthylazo)-6-nitronaphthalene-4-sulphonate, gives a purple-red colour
with calcium, magnesium and zinc ions in alkaline solution. When
these ions are absent, the solution is blue.

3.25.1 Mordant Black Indicator Mixture - Triturate 0.2 g of moraanr

black 11 with 100 g of sodium chloride to a fine powder.

3.26 Murexide - Chemictilly described as ammonium purpurate, gives

an orange colour with copper, yellow colour with nickel and cobalt
(PH 10 to 11) and red colour with calcium ( PH 11) in alkaline solution.
When these ions are absent, the solution is purple.

3.26.1 Murexide Indicator Mixture - Triturate 1 g of murexide with

100 g of potassium sulphate, anhydrous to a fine powder.

3.27 Patton and Reeder’m Indicator - Sss 3.23.

8 ’
 IS : 2263 - 1979

3.28 Pyridylazonaphthol - ChemicaIly described as l-( 2-pyridylazo)-

!?-naphthol, gives a yelli)w colour with copper ions in acid solution.
When these ions are absent, the solution is red.

3.28.1 Pyridyln~onuphthol Indicator Solution - 1 g/l. Dissolve 0.25 g

of pyridylazonaphthol in 250 ml of methyl alcohol.

3.29 Sodium Alizarin Sulphonate - Chemically described as sodium

1,2-dihvdroxy-9, IO-anthraquinone-3-sulphonate, gives a bluish-red
lake with thorium and aluminium ions, at about PH 4’0. When these
ions are absent, the solution is yellow.

3.29.1 Sodium Alitarin Sul~honatt Indicator Solution - 1 g/l. Dissolve

Q25 g of sodium alizarin sulphonate in 250 ml of water.

3.30 Thoron - Chemically described as sodium 2-( 2-hydroxy-3,6-

disulpho-l-naphthylazo) benzenearsonate, gives a red colour with barium;
thorium and uranyl ions in acid solution. When these ions are absent,
the solution is yellow.

3.30.1 Thoron Indicator Solution - 5 g/l. Dissolve 1’25 g of thoron in

250 ml of water.

3.31 Xylenol Orange - Chemically described as 3,3’-bis [ NN-di

( carboxymethyl ) aminomethyl-o-cresolsulphonphthalein, gives a raddish-
purple colour with mercury, lead and zinc ions in acid solution. When
these ions are absent, the solution is yellow.

3.31.1 Xylenol Orange Indicator Solution - 1 g/l. Shake 0’25 g of

xylenol orange with 250 ml of water and filter if necessary.

SECTION 3 INDICATORS FOR NON-AQUEOUS

TITRATIONS

3.32 Alkali Blue 6B - Chemically described as sodium triphenylpara-

rosaniline monosulphonate; when used for titration in benzene solution,
it changes from red ( basic ) to blue ( acidic ).
3.32.1 Alkali Blue 6B Indicator Solution - 20 g/l. Extract 2 g of alkali
blue 6B with rectified spirit in a Soxhlet apparatus.. Filter the solution if
necessary and dilute to 100 ml with rectified spirit.
3.33 Crystal Violet - Chemically described as hexamethylpararosani-
line hydrochloride, when used for titration in non-aqueous media, it
changes from violet ( basic ) through bluish-green ( neutral ) to yellowish-
green ( acidic ).

9
P

IS t 2263 - 1979

3.33.1 Crystal Violet Indicator Solution - 5 g/l. Dissolve 1.25 g of crystal

violet in 250 ml of giacial acetic acid.

3.34 I-Naphtholbenzein

3.34.1 I-Naphtholbcneain Indicator Solution ( Acetic Acid ) - 2 g/l. Dissolve

0’5 g of 1-naphtholbenzein in 250 ml of glacial acetic acid. When used
for titration in non-aqueous media, it changes from green ( basic ) through
. orange ( neutral ) to yellow ( acidic ).
3.34.2 I-JVaphtholbenzcin Zndicator Solution ( Propan-P-01 ) - 10 g/l.
Dissolve 2’5 g of I-naphtholbenzein in 250 ml of propan-2-01. When
used for titration in non-aqueous media it changes from green ( basic ) to
yellow orange ( acidic ).
3.35 Quinaldine Red - Chemically described as 2- ( 4_dimethylamino-
styryl ) quinoline ethiodide; when used for the titration of bases with
perchloric acid in glacial acetic acid, it changes from magenta to almost
colourless. .
3.35.1 ainaldine Red Indicator Solution - 1 g/l-. Dissolve O-25 g of
quinaldine red in 250 ml of methanol.

SECTION 4 OXIDATION AND REDUCTION INDICATORS

3.36 Barium Diphenylamine-4-Sulphonate - Changes from colour-

less to violet at a transition emf of $0’84 V.
3.36.1 Barium Diphenylamine-4-Sulphonate Indicator Solution - 7:5 g/l.
Dissolve 0.75 g of barium diphenylamine-4-sulphonate in water and
dilute to 100 ml.
3.37 l,lO-Pheninthroline - A solution of the complex with ferrous
ions changes from red to blue at a transition emf of +i.OSV.

3~37.1 IJO-Phcnanthrolinc Ferrous Complex Indicator Solution - 17 g/l-

Dissolve 1’49 g of I,lO-phenanthroline in 70 ml of water containing 0.7 g
of ferrous sulphate. Dilute to 100 ml with water.

SECTION 5 Ml$CELLANEOUS INDICATORS

3.38 Ammonium Ferric Snlphate - Gives a deep red colour with

thiocyanate ions in acid solutions.

10
 IS : 2263 - 1979

3.38.1 Ammonium Ferric Sul~hate Indicator Solution - 100 g/l. Dissolve

25 g of ammonium ferric sulphate in 250 ml of water. Allow the
solution to settle and decant if necessary. If this does not give a clear
aolution add a few drops of 5 N nitric acid.
3.39 4.Dimetbylaminobenzylidenerbodanine - Changes from, very
pale yellow to red in the presence of silver ions at a PH of less than 12.
3.39.1 I-Dimcthylaminotwnzylidcncrhodanine Indicator Solution - 0.2 g/l.
Dissolve 50 mg of 4-dimethylaminobenzylidenerhodanine in 250 ml of
acetone.
3.40 Methylene Blue - Chemically described as tetramethylthionine
chloride dihydrate. Phase change indicator in the titration of d’eter-
gents.
3.40.1 Methylenc Blue Indicator- Solution - 0.15 g/l, Dissolve 38 mg of
methylene blue in 250 ml of water.
3.41 Potassium Chromate - Gives a red precipitate with silver nitrate
in neutral solution.
3.41.1 Potassium Chromate Indicator Solution - 50 g/l. Dissolve 12.5 g
of potassium chromate in 250 ml of water.
3.42 Starch - Gives a blue colour with free iodine in the presence of a
soluble iodide.
3.42.1 Starch Indicator Solution - 5 g/l. Triturate 0’5 g of soluble
starch with 5 ml of water and add this, with constant stirring, to
sufficient boiling water to produce about 100 ml.. Boil for a few minutes
and cool.

SECTION 6 ADSORPTION INDICATORS

3.43 Amaranth - Chemically described as trisodium l-( 4-sulpho-l-

naphthylazo )-2-naphthol-3, 6-disulphonate. When used for, the titration
of iodine and iodides with potassium iodate, the colour changes from
orange red to yellow.
3.43.1 Amaranth Indicator Solution - 2 g/l. DissoIve.0’5 g of amaranth
in 250 ml of water.
3.44 Dicblorofluorescein - Chemically described as 2,7-dichloro-3, 6-
fluorandiol; when used in the titration of’ a chloride with silver nitrate.
at ~JH 5 to 8 it colours the precipitate orange. When precipitation is
complete, the colour of the precipitate is pink.

11
 ---

IS : 2263 - 1979

3.44.1 DichloroJIuoresccin Indicator Solution - 1 g/l. Dissolve 0.25 g of

dichlorofluorescein in 250 ml of rectified spirit.

3.45 Phenosafranine - Chemically described ai 3,7-diamino-5-phenyl-

phenazonium chloride; when used in the titration of a chloride or
bromide with silver nitrate in acid solution, it colours the precipitate
red. When precipitation is complete, the kolour of the precipitate is
blue.

3.45.1 Phenosafranine Indicator Solution - 2.5 g/l. Dissolve 0.25 g of

phenosafranine in water and dilute to 100 ml.

12
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531'5.Ward No. 29, R.G. Barua Road, 5th Byelane, 3 31 77
GUWAHATI 781003
5-B-56C L. N. Gupta Marg ( Nampally Station Road ), 23 1083
HYDERABAD 500001
6 34 71
R14 Yudhister Marg. C Scheme, JAIPUR 302005
( 6 98 32
21 68 76
117/418 B Sarvodaya Nagar, KANPUR 208005
I 21 8292
Patliputra Industrial Estate, PATNA 800013 6 23 05
T.C. No. 14/l 421. University P.O.. Palayam 16 21 04
TRIVANDRUM 695035 16 21 17
inspection Offices ( With Sale Point ):
Pushpanjali, First Floor, 205-A West High Court Road, 2 51 71
Shankar Nagar Square, NAGPUR 440010
Institution of Engineers ( India ) Building, 1332 Shivaji Nagar, 5 24 35
PUNE 411005

*Sales
Street. Calcutta in Calcutta is at 5 Chowringhse
Office700072 Approach, P. 0. Princep 27 68 00

tSales Office in Bombay is al Novelty Chambers, Grant Road, 89 66 28

Bombay 400007
Bangalore
$Sales 560002
Office in Bangalore is at Unity Building, Narasimharaja Square, 22 36 71

Reprography, Unit , BIS, New Delhi, India