Thermocycler 207

Mastercycler® pro

vapo.protectTM technology*
Evaporation in a PCR reaction vessel not only limits the ability to
work with low reaction volumes, but also makes it very difficult to
obtain reproducible results. For the Mastercycler pro it has been
Eppendorf’s aim to protect the PCR reaction from evaporation.
While common PCR instruments press a metal plate onto the
PCR consumables, the vapo.protectTM lid covers the PCR con-
sumables with a cushion that fits to the shape of the consuma-
bles – no matter which consumables you use. The snug fit of the
cushion, the high pressure of the lid and the excellent heating
capabilities of the lid reduce evaporation to a minimum.
Evaporation leads to increasing concentrations of the PCR
master mix components, especially primers. This, in turn, leads
to non-specific binding. Due to the ongoing evaporation, this
increased concentration is even changing during the experiment
and between experiments – a true nightmare for any scientist
who tries to keep variables to a minimum.
4 | Instruments | PCR
Description
Fastest speeds, highest precision, easy usage and absolute reli-
ability united in a flexible concept – that is the Mastercycler pro.
Today’s requirements for PCR in your lab are defined by today’s
research project. Tomorrow, new questions are arising and new
experiments have to give the answers. That is why only an open
and flexible system fit into today’s lab. Eppendorf’s Mastercycler
pro offers ultimate flexibility:
– choose from three block formats
– operate the Mastercycler pro in three different configurations
– use any plate, tube or strip you want
Unsurpassed speed can be achieved with Eppendorf’s Impulse
PCR, a device driven hot-start function that increases heating
rates to 8°C/s.

Gradient with SteadySlope® technology

The gradient option of the Mastercycler pro features the
SteadySlope® technology to ensure that the heating and cooling
ramp rates are identical in both gradient and normal operation.
Thus, you can expect identical temperature control characteris-
tics in both optimization and routine experiments. Programming
the gradient with the Control Panel and the CycleManager pro is
easy and intuitive – so that novice users can get started quickly
and safely.

Certified Quality
– Individual, documented quality control certificate
– Calibration accordingly to national and international standards:
NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK)
Further information under
– UL listed www.eppendorf.de/mastercyclerpro
* Patents pending
For additional information see
“Application tips for PCR” on page 282.

Eppendorf AG · 22331 Hamburg · Germany · Phone +49 40-53801-0 · Fax +49 40-53801-556
E-Mail: eppendorf@eppendorf.com · Internet: www.eppendorf.com
 208 Thermocycler

Mastercycler® pro

Product Features
4 | Instruments | PCR

‡ Extremely fast heating and cooling rates

‡ Ultimate reduction of evaporation
‡ Gradient blocks with SteadySlope® technology
‡ Intuitive graphic programming
‡ Display to indicate cycler number in a network
‡ Upgradable to real-time PCR
‡ 2 years warranty
‡ Optional self-test dongle available
‡ Stand alone system: Fits in every lab. Operated by Control
Panel. Upgradeable to real-time PCR. Expandable to a Mini
Satellite System or network
‡ Mini Satellite System:
Operated by Control Panel. Control up to 5 units with one
Control Panel. Combine any block format. Expandable to a
network
‡ Computer Network: Combine up to 30 units for ultimate
throughput. Operated by a computer and the CycleManager
prosoftware. Benefit from a client / server architecture and
full software functionality
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover
device is licensed under US 5,552,580 and foreign equivalents. The user of the Eppendorf Mastercycler
pro might require additional rights for kits, reagents and other components required for his/her appli-
cation. Such accompanying rights for these kits, reagents and other may be obtained by the respective
holder of such rights. No rights are conveyed expressly, by implication or estoppel to any patents on
real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent
or kit. Mastercycler pro upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler
License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S.
counterparts.

Technical specifications
Description Mastercycler pro Mastercycler pro S Mastercycler pro 384
Thermoblock Aluminum Silver Aluminum
96 x 0.2 ml PCR tubes or 1 PCR plate 8 x 12 (unskirted,
Sample capacity: 1 PCR plate 384
semi-skirted, skirted – according to SBS standard)
Temperature control range of the block: 4–99 °C 4–99 °C 4–99 °C
Temperature control mode: Fast, Standard, Safe; all available in gradient mode
Heating technology of the block: Peltier elements, Triple Circuit Technology
Gradient block: Over 12 rows Over 12 rows Over 24 rows
Gradient range: 1–20 °C 1–24 °C 1–20 °C
Gradient temperature range: 30–99 °C 30–99 °C 30–99 °C
Lid temperature range: 37–110 °C 37–110 °C 37–110 °C
Lid descent and closing pressure: vapo.protect™ technology, with Thermal Sample Protection
Block homogeneity: 20–72 °C ±0.3 °C, 90 °C ±0.4 °C
Control accuracy: ± 0.2 °C ± 0.2 °C ± 0.2 °C
Heating rate* ca. 4 °C/s ca. 6 °C/s ca. 4 °C/s
Cooling rate* ca. 3 °C/s ca. 4.5 °C/s ca. 3 °C/s
Interfaces 1 x Centronics®, 1 x RS-232, Control panel, one each of CAN_in/CAN_out
Dimensions (W x D x H): 26 x 41.5 x 37 cm 26 x 41.5 x 37 cm 26 x 41.5 x 37 cm
Weight 18.5 kg (40.8 lbs) 18.5 kg (40.8 lbs) 18.5 kg (40.8 lbs)
Power supply 115 V or 230 V, 50–60 Hz 115 V or 230 V, 50–60 Hz 115 V or 230 V, 50–60 Hz
Power consumption: 950 W 950 W 950 W
* Block temperature.

Technical specifications subject to change.

 Thermocycler 209

Mastercycler® pro

Programming features

4 | Instruments | PCR
‡ Highest degree of flexibility, intuitive graphic
programming
‡ Variable ramp rates
‡ Emulation mode for temperature control speed and
protocols of the original Mastercycler family
‡ Time and temperature increments
‡ Pause command
‡ Programmable alarm sound
‡ Freely configurable auto-restart dialogue

Regulatory documentation
Compliance with GMP/GLP guidelines is becoming increasingly
important, particularly in the pharmaceutical and biotechnology
industries. The Mastercycler pro system’s programming and user
management system provides a GLP-conforming environment
for your PCR assays.

Program and user administration features

‡ One administrator to manage user and service functions

‡ Easy addition of new users with limited rights
‡ Ability for users to change their passwords; no
administrator access to user passwords
‡ Password protection against unauthorized login
‡ A report file for each run, including total temperature
profiling
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover ‡ Development, testing and validation of the software using
device is licensed under US 5,552,580 and foreign equivalents. The user of the Eppendorf Mastercycler
pro might require additional rights for kits, reagents and other components required for his/her appli- certified standards
cation. Such accompanying rights for these kits, reagents and other may be obtained by the respective
holder of such rights. No rights are conveyed expressly, by implication or estoppel to any patents on
real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent
or kit. Mastercycler pro upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler
License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S.
counterparts.

Technical specifications of the Control Panel

Number of Mastercycler pro cyclers that can be controlled 1–5
Memory capacity 16 MB, > 700 programs
Memory expansion 2x USB 2.0
Number of user-defined folders > 100
Dimensions (W x D x H) 25 x 16.5 x 11 cm
Interfaces 1 x PS/2 mouse, 2x USB 2.0
Weight 1.2 kg
Power supply via the connected thermal module

Ordering information for other voltage options

begins on page 314.

Eppendorf AG · 22331 Hamburg · Germany · Phone +49 40-53801-0 · Fax +49 40-53801-556
E-Mail: eppendorf@eppendorf.com · Internet: www.eppendorf.com
 210 Thermocycler

Mastercycler® pro and CycleManager pro

Product features
4 | Instruments | PCR

‡ Network and control up to 30 Mastercycler pro thermal

cyclers with a PC; mix and match models of choice
‡ Multi-Mastercycler-Networking with a PC
‡ Intuitive Windows-like interface
‡ Icon-based creation and edit of programs
‡ Simultaneous control of all or selected Mastercycler pro
cyclers
‡ GLP-conformity documentation of all PCR experiments
‡ User password protection
‡ Real-time acquisition of temperature data
‡ Easy installation and de-installation
‡ Compatible with Windows
‡ Easy transfer of PCR programs from the Control Panel
to the PC
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover
device is licensed under US 5,552,580 and foreign equivalents. The user of the Eppendorf Mastercycler
pro might require additional rights for kits, reagents and other components required for his/her appli-
cation. Such accompanying rights for these kits, reagents and other may be obtained by the respective
holder of such rights. No rights are conveyed expressly, by implication or estoppel to any patents on
real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent
or kit. Mastercycler pro upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler
License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S.
counterparts.

Ordering information
Description Order no.
Complete Mastercycler® pro package:
Mastercycler® pro and Control Panel, 230 V, 50–60 Hz 6321 000.515
Mastercycler® pro S and Control Panel, 230 V, 50–60 Hz 6325 000.510
Mastercycler® pro 384 and Control Panel, 230 V, 50–60 Hz 6324 000.516
Individual Mastercycler® pro modules:
Control Panel, incl. connection cable 6320 000.007
Mastercycler® pro*, 230 V, 50–60 Hz 6321 000.019
Mastercycler® pro S*, 230 V, 50–60 Hz 6325 000.013
Mastercycler® pro 384*, 230 V, 50–60 Hz 6324 000.010
Accessories
CAN_Bus connection cable, 50 cm 5341 612.006
CAN_Bus connection cable, 150 cm 5341 611.000
Self Test Dongle 6320 071.001
MultiMediaCard, 64 MB 5075 780.003
CycleManager pro, incl. installation manual, online help, and connection cable 5349 810.001
CycleManager pro, incl. installation instructions, online help 5349 820.007
* A Control Panel or CycleManager pro software (both sold separately) is required for operation; CAN_Bus connection cables are required to link cyclers together as a network.

Technical specifications are available at

www.eppendorf.com/mastercyclerpro

Technical specifications subject to change.

 Thermocycler 211

Mastercycler®, Mastercycler® gradient and Mastercycler® personal

4 | Instruments | PCR
Product features Description
The Mastercycler gradient sets the standard for optimization of
‡ Universal block PCR reaction conditions. A freely programmable temperature
‡ Utmost flexibility, simple programming: gradient function, gradient range of up to 20 °C enables the optimization not only
adjustable ramp rates, time and temperature increments, of the annealing temperature, but also of all temperature steps of
incubation mode, in situ mode, link function, a PCR protocol for even the most demanding applications.
pause function SteadySlope technology ensures optimal, constant heating and
‡ RS-232 and printer interface cooling rates, which means that results of gradient experiments
‡ Auto-restart option in the event of a power failure can be transferred simply and precisely to routine applications.
‡ SteadySlope® and Triple Circuit Technology The entire Mastercycler family incorporates the personal card
‡ Personal card system system: chip-based memory cards that enable a simple and
‡ Two-year warranty reliable transfer of your individual protocols between different
Mastercycler workstations and ensure unlimited memory
expansion.
The universal block incorporates an expansive range of different
tube types in both the Mastercycler and Mastercycler gradient:
use 96 x 0.2 ml tubes, 77 x 0.5 ml tubes or one 96-well PCR
plate—without having to change the block.

Further information under

www.eppendorf.com/mastercyclerfamily

Ordering information for other voltage options

begins on page 314.

Eppendorf AG · 22331 Hamburg · Germany · Phone +49 40-53801-0 · Fax +49 40-53801-556
E-Mail: eppendorf@eppendorf.com · Internet: www.eppendorf.com
 212 Thermocycler

Mastercycler®, Mastercycler® gradient and Mastercycler® personal

Technical specifications
4 | Instruments | PCR

Description Mastercycler® / Mastercycler® gradient Mastercycler® personal

Sample capacity 96 x 0.2 ml PCR tubes or 25 x 0.2 ml PCR tubes or
77 x 0.5 ml PCR tubes or 16 x 0.5 ml PCR tubes or
1 x 96-well PCR plate 1 multiwell PCR plate 5 x 5
Temperature control range: 4–99 °C 4–99 °C
Temperature distribution across the block:
20–72 °C ±0.6 °C ±0.6 °C
95 °C ±1.0 °C ±1.0 °C
Block homogeneity
20–72 °C ≤ ±0.4 °C ≤ ±0.4 °C
95 °C ≤ ±0.5 °C ≤ ±0.5 °C
Regulation accuracy per well: ± 0.2 °C ± 0.2 °C
Block ramp speed: approx. 3 °C/s (heating) approx. 3 °C/s (heating)
approx. 2 °C/s (cooling) approx. 2 °C/s (cooling)
No. of programs: 100 on device; 100 on device;
approx. 10 on personal card approx. 10 on personal card
Dimensions (W X D X H): 26 x 41 x 27 cm 18 x 35 x 25 cm
Weight: 12.4 kg 6.8 kg
Power supply: 230 V, 50–60 Hz 230 V, 50–60 Hz
Power consumption: 500 W 150 W

Ordering information
Description Order no.
®
Mastercycler gradient 5331 000.010
230 V, 50 Hz, with heated lid and 1 personal card
Mastercycler® 5333 000.018
230 V, 50 Hz, with heated lid and 1 personal card
Mastercycler® personal 5332 000.014
230 V, 50 Hz, with heated lid and 1 personal card
Personal card 5332 300.018
for Mastercycler® gradient, Mastercycler® and Mastercycler® personal
Eppendorf CyclerManager software 5339 810.003
incl. installation manual, online help and connection cable
IFC Thermal Cycler 5332-F*1 5332 000.405
*1 Available in selected countries only.

Practice of the patented polymerase chain reaction (PCR) process

requires a license. The Mastercycler is an Authorized Thermal
Cycler and may be used with PCR licenses available from Applied
Biosystems. Its use with Authorized Reagents also provide a limited
PCR license in accordance with the label rights accompanying such
reagents.

Ordering information for other voltage options

begins on page 314.

Technical specifications subject to change.

 Thermocycler 213

in situ Adapter

Description

4 | Instruments | PCR
The in situ Adapter can be used to convert your Mastercycler®
or Mastercycler gradient to an in situ device as easily as if you
were to insert a PCR plate. And the quality of the heat transfer is
equally as high!
With precise block homogeneity, the in situ Adapter has room
for up to four standard glass slides. The productivity-enhancing
in situ mode of the Mastercycler software frees up the time you
would otherwise spend on optimization.

Technical specifications
Sample capacity Max. 4 glass slides
76 x 26 mm
Temperature range 4–99 °C
Temperature distribution ±0.6 °C
across the adapter ±1.0 °C
Temperature control speed ≥ 1.0 °C/s (heating)
Product features measured on the adapter ≥ 0.7 °C/s (cooling)

‡ Easy to handle
‡ Perfect fit for optimal heat transfer Ordering information
‡ Software supported, easy to program, extremely flexible
Description Order no.
‡ Can accommodate four standard glass slides
‡ Transparent covering hood in situ Adapter 5331 220.002
for Mastercycler® gradient
and Mastercycler®

Temperature Verification System - Single Well

Description
The Temperature Verification System - Single Well for the entire
Mastercycler, Mastercycler ep and Mastercycler pro families
enables simple and rapid validation and calibration of block tem-
perature. The program for automatic measurement and calibra-
tion is integrated into the thermocycler software, thus providing
an accurate and precise system that meets all documentation
and detection requirements in the laboratory.

Ordering information
Description Order no.
Temperature Verification 0055 000.298
System - Single Well
Product features

‡ Sensor for 0.2 ml tube position

‡ Optional: sensor for 384-well block, sold separately
‡ Simple, rapid validation and calibration
‡ Autocalibration with Mastercycler software, version 2.10
or higher
‡ Can be third-party calibrated

Eppendorf AG · 22331 Hamburg · Germany · Phone +49 40-53801-0 · Fax +49 40-53801-556
E-Mail: eppendorf@eppendorf.com · Internet: www.eppendorf.com