PRESENTATION OF RESULTS

DISTILLED
G WATER 0.2 M 0.15 M 0.10 M 0.5 M
T A T A T A T A T A

1 26.5% 0.576 0.3 2.5 0 2.5 1.4 1.86 37.1 0.431

2 27.1% 0.568 0.3 2.5 1.5 1.84 2.9 1.53 35.3 0.452

3 22.7% 0.645 0 2.5 0 2.5 0 2.5 20.3 0.692

4 16.5% 0.785 0 2.5 0 2.5 0 2.5 16.8 0.776

5 28.3% 0.54 0.6 2.301 0.7 2.154 0.1 2.5 23 0.639

6 14.5% 0.839 0 2.5 0 2.5 0.2 2.5 3.3 1.488

7 19.1% 0.72 0.3 2.5 0 2.5 0 2.5 1.6 1.793

8 11.8% 0.929 0 2.5 0 2.5 0 2.5 22.7 0.644

AVE. 20.81% 0.70 0.19% 2.48 0.28% 2.37 0.58% 2.30 20.01% 0.86

Table 1. Red Blood Cell in Different Concentration

This table presents the data gathered from the 8 groups who tested the blood in different
concentrations of NaCl (0.2 M, 0.15 M, 0.10 M, 0.5 M) in the form of transmittance and
absorbance and also it shows the computed mean of all concentrations including distilled water.
 300.00%

250.00% 2.48
2.37
2.3

200.00%

150.00% ABSORBANCE
TRANSMITTANCE

100.00% 0.86
0.7

50.00%

20.81% 20.01%
0.00% 0.19% 0.28% 0.58%
0 0.2 M 0.15 M 0. 10 M 0.05 M

Figure 1. Absorbance and Transmittance in Red Blood Cell

As shown in the figure 1, since the distilled water has less solute, this is considered as
hypotonic. These hypotonic solutions lead the cell to swell and eventual rupture or lyses if the
resultant osmotic movement of water is great enough (Goodhead, 2017) In the case of red
blood cells, this is referred to as hemolysis and is an absolute value, and then it is represented
by zero (0). In transmittance, concentration 0.2 acquired the lowest percentage of 0.19%
whereas it also got the highest number of absorbance which is 2.48. On the other hand, aside
from the distilled water which ranked the first in transmittance, concentration 0.05 has its peak in
20.01% while on the part of absorbance it only obtained 0.86. It proves that the higher the
absorbance, the lower the transmittance will likely occur.

According to the Beer-Lambert Law, if all the light passes through a solution without any
absorption, then absorbance is zero, and percent transmittance is 100%. If all the light is
absorbed, then percent transmittance is zero, and absorption is infinite.

In the study of Mehmet Uyuklu et. al ,(2011), it is clearly stated that light absorbance by
the red blood cell importantly contributes to the behavior of light passing through a suspension
of these cells, with higher absorbance resulting in reduced light transmittance. Red blood cell
hemoglobin content is also an important determinant of absorbance and, as well in the level of
light transmittance. The absorption spectrum of hemoglobin is clearly a critical factor
determining light transmittance through RBC suspensions. Hemoglobin which is the red blood
cell is an effective light absorbent, especially at wavelengths below 600 nm.
Reference

Clark, J. (2019). The Beer-Lambert Law. Chemistry Libre Texts. Retrieved from
https://chem.libretexts.org/Bookshelves/Physical_and_Theoretical_Chemistry_Textbook_Maps/
Supplemental_Modules_(Physical_and_Theoretical_Chemistry)/Spectroscopy/Electronic_Spect
roscopy/Electronic_Spectroscopy_Basics/The_Beer-Lambert_Law
https://teaching.shu.ac.uk/hwb/chemistry/tutorials/molspec/beers1.htm
Uyuklu, M., Canpolat, M., Meiselman, H., & Baskurt, O. (2011). Wavelength selection in
measuring red blood cell aggregation based on light transmittance. Journal of Biomedical
Optics. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221720/
Goodhead, L. & MacMillan F. (2017). Measuring osmosis and hemolysis of red blood cells.
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https://www.physiology.org/doi/full/10.1152/advan.00083.2016