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Alex Aguilar

BEER’S LAW LAB

Create your own data tables and graphs on separate


piece of paper. Make sure partner's name are on
the paper
INTRODUCTION
1. Explore the Beer’s Law screen for a few minutes. Try to figure out what all of
the controls show and do.

2. How does Concentration affect how much light is absorbed and transmitted
through the solution?
Concentration affects how much light is absorbed and transmitted through and transmitted
through the solution because as it increases absorbance also increases and light transmitted
decreases.

INVESTIGATING ABSORPTION AND CONCENTRATION


1. Predict what a graph of absorbance versus concentration would look like.
Sketch your prediction.
Prediction

Can’t edit graph but basically as absorbance increases, so does


concentration. (papers with graphs will be given in person)
2. Choose a solution from the simulation and measure the Absorbance for
different concentrations on the preset wavelength setting.

Data from the Simulation

1
Concentration
Abs
________M
0 0
100 0.4
200 0.90
300 1.32
400 1.62
(graph is also exponential bc as absorbency increases, so does concentration.)

3. How does your second graph compare to your prediction?

The prediction and results were strikingly similar.

4. Based on Beer’s Law (A = εlC, A = absorbance, ε = molar absorptivity, l =


pathlength and C = concentration), do you expect using different
wavelengths of light would change the way your previous graph looks? Why or
why not?

No, because concentration will always increase with absorbance.

INVESTIGATING ABSORPTION AND WAVELENGTH


1. a. Compare three solutions of different colors with the same pathlength (width
of container).

Preset Wavelength: Variable Wavelength:


Simulation default Set to same color as
setting solution
Solution Solution Color Beam Color Value (nm) Abs Beam Color Value (nm) Abs
549 nm 0.39 639 nm 0.14
CoCl2 Cobalt
Chloride
KMnO4 544 nm 0.10 411 nm 0.01
Potassium
Permangana
te
K2CrO4 411 nm 0.27 612 nm 0.00
Potassium
Chromate

b. What combinations give the most absorbance? Why?

The peach colored one with 549 nm because the green is absorbing the peach.
c. How are beam color, solution color, and absorbance related?
The darker the color, the higher the absorbance.

λ(nm) Abs

2. a. Choose a solution and keep concentration and pathlength constant as


you graph the absorbance for different wavelengths.

Solution: KMnO4

380 580 780


Wavelength (nm)
(graph will be given in person)
b. What is the value for the “preset” wavelength for your solution? Mark
this point on your graph.
The “preset” wavelength value for KMnO4 is 544 nm.

c. Why do you think the “preset” wavelength is the best wavelength to


use for this solution?
It has the most absorbency. (2.0)

3. Compare your absorbance spectrum sketch with a group that chose a different
solution. Would you use the same wavelength of light to do spectroscopy
experiments with different colored solutions? Why or why not?
No because all solutions are different.
4. In a lab experiment monitoring the change in concentration of a reddish-brown
substance, FeNCS2+, a wavelength of 455 nm is used.
a. Does this wavelength agree with your conclusions about beam
color, solution color, and absorbance above? Why or why not?

Yes, the reddish-brown substance will absorb the blue wavelength.

b. What other wavelengths might you consider using for FeNCS2+


spectroscopy?
A wavelength around lower 500s.

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