BIOSTAT® Qplus
12-fold Screening Fermentor | Bioreactor
Screening Fermentor|Bioreactor System
System Description
The BIOSTAT® Qplus is a new generation of
Fermentor|Bioreactor systems designed for
parallel operation with high throughput
capability.
The combination of a newly engineered
control system launches small scale cultiva-
tion vessels into a new era of biotechnology
screening. The BIOSTAT® Qplus has the
capability to control fully independently
up to twelve culture vessels with minimal
manual operation.
Application-driven configured packages
for microbial and cell culture provide
everything to get started immediately.
The BIOSTAT® Qplus is available with
scaleable culture vessel working volumes
of 0.5 L and 1 L.
The BIOSTAT® Qplus is delivered with a
completely configured software package.
A 3-fold, 6-fold, 9-fold system can be easily
extended with a 3-fold vessel |supply tower
extension kit up to a 12-fold system without
expensive software extensions.
For further enhancement of system perform-
ance, a powerful supervisory process control
software MFCS | DA for extended visualiza-
tion, data acquisition and trend display is
included.
The BIOSTAT® Qplus is ideal for:
– Process development
– Process optimization
– Up- and Down-scale experiments
– Strain and Cell line characterization
– Quality control
– Parallel production process control
Applications
– Growth studies of microbial, mammalian,
insect and plant cells
– Culture media composition and
optimization
– Upscale migration, i. e. transition from
shaking flasks
– Downscale of production process for
process optimization
– Small scale protein and Mab expression
– High cell density cultivation
Features
– Space saving tower design
– Graphical user interface with touch
screen operation
– Fully independent vessel control
– Integrated thermostat system
– Integrated gassing system with O2 supple-
mentation for microbial packages
– Integrated 4-gas mixing system with
Sparger and Overlay aeration for cell
culture packages
– Top drive agitation system with
maintenance-free motor
– Pre-configured software for up to 12-fold
operation
– Control of pH, DO, temperature, foam|level,
substrate addition, gas mixing and gas
flow rate
– Scaleable vessel design
– Optional Turbidity or Redox measurement
– Easy upgrade of cell culture packages for
multipurpose use
– Inclusive Supervisory Process control
software
Technical Specification

Space requirements|Environmental conditions Dimensions

Bench space requirement|weight|0.5 L culture vessel
3-fold | 6-fold | 9-fold | 12-fold [W + H + D] 850 | 1320 | 1860 | 2400 + 900 + 750 [mm] | 95 | 155 | 216 | 278 [kg]
Bench space requirement|weight|1 L culture vessel
3-fold | 6-fold | 9-fold | 12-fold [W + H + D] 1100 | 1800 | 2550 | 3300 + 900 + 800 [mm] | 114 | 194 | 274 | 355 [kg]
Space requirement autoclave d + H 0.5 L
per culture vessel | 3-fold vessel assembly with tray 170 + 340 | 480 + 340 [mm]
Space requirement autoclave d + H 1 L
per culture vessel | 3-fold vessel assembly 240 + 500 | 460 + 500 [mm]
Ambient temperature|relative humidity (non condensating) 5–40°C|85%
Control Tower
Housing material Stainless steel AISI 304
Display Touch Screen 15"
Resolution 1024 + 600 dpi
Communication Control Tower|Supply Tower |Host Ethernet|Ethernet
Measurement ranges
Agitation motor speed 50 –1200 rpm
Temperature 0 – 150 °C
pH 2 – 12
pO2 0 – 100%
Turbidity (optional) 0 – 6 AU
Redox (optional) –1000–1000 mV
Supply Tower Microbial Version Cell Culture Version
Housing material Stainless steel AISI 304
Gassing System Air areation with O2 supplementation 4-gasmixing for Air N2, O2 CO2
Sparger outlet Sparger and Overlay outlet
Outlet design Hose connector OD 6 mm Hose connector OD 6 mm
Flowmeter
Gas flow range "Sparger" 0.5 L|1 L 0.1–1|0.16–1.6 [l/min] 0.005–0.05|0.016–0.166 [l/min]
Gas flow range "Overlay" 0.5 L|1 L –– 0.05–0.5|0.16–1.6 [l/min]
Accuracy +/– 5% FS
Thermal Mass Flow Controller (optional)
Flow range 0.03–1.5 slpm 0.003–0.150 slpm
Accuracy +/– 1% FS
Integrated Pumps
Pump head Watson Marlow 102R
Rotation speed 20 rpm
Flow rate integrated pumps 0.04–33.2 [ml/min] (tube dependent)
Thermostate System
Temperature control range 8 °C above cooling water to 60 °C
Connections to culture vessel Quick couplings
External Connections per Vessel
Feed pump connection|External input 0 – 10 V|0 – 10 V
Culture vessel 0.5 L 1L
Design Jacketed glass vessel with stainless steel head pleate
Total volume 0.75 L 1.6 L
Working volume 0.15–0.5 L 0.4–1 L
Headpleate ports total 19 mm | 12 mm | 6 mm –|6|4 3|2|6
pO2 electrode Polarographic
pH electrode Gel filled
Temperature probe Pt100
Material (product wetted parts) Borosilicat glass|Stainless steel AISI 316 L|EPDM
Volume storage bottles 250 mL
Utilities Requirements|Housing Connection
Power supply 120 VAC or 230 VAC
Gasses Controlled @ 1.5 barg dry, particle and oil free|hose connector OD 6 mm
Water Controlled @ 2 barg|hose connector OD 10 mm
Drain Gravity drain with zero backpressure required |hose connector OD 10 mm
Ordering Information

Description BIOSTAT® Qplus Microbial Version BIOSTAT® Qplus Cell Culture Version
Culture vessel 0.5 L 1L 0.5 L 1L
Cat. No. 120 VAC
BIOSTAT® Qplus 3-fold version RQ-3M5DLOTRDG1 RQ-3M01LOTRDG1 RQ-3C5DLETSDG1 RQ-3C01LETSDG1
BIOSTAT® Qplus 6-fold version RQ-6M5DLOTRDG1 RQ-6M01LOTRDG1 RQ-6C5DLETSDG1 RQ-6C01LETSDG1
BIOSTAT® Qplus 9-fold version RQ-9M5DLOTRDG1 RQ-9M01LOTRDG1 RQ-9C5DLETSDG1 RQ-9C01LETSDG1
BIOSTAT® Qplus 12-fold version RQ12M5DLOTRDG1 RQ12M01LOTRDG1 RQ12C5DLETSDG1 RQ12C01LETSDG1
3-fold MO upgrade to max 12 fold system
without digital controller RQ-3M5DLOTRDG1E RQ-3M01LOTRDG1E RQ-3C5DLETSDG1E RQ-3C01LETSDG1
Cat. No. 230 VAC
BIOSTAT® Qplus 3-fold version RQ-3M5DLOTRDG2 RQ-3M01LOTRDG2 RQ-3C5DLETSDG2 RQ-3C01LETSDG2
BIOSTAT® Qplus 6-fold version RQ-6M5DLOTRDG2 RQ-6M01LOTRDG2 RQ-6C5DLETSDG2 RQ-6C01LETSDG2
BIOSTAT® Qplus 9-fold version RQ-9M5DLOTRDG2 RQ-9M01LOTRDG2 RQ-9C5DLETSDG2 RQ-9C01LETSDG2
BIOSTAT® Qplus 12-fold version RQ12M5DLOTRDG2 RQ12M01LOTRDG2 RQ12C5DLETSDG2 RQ12C01LETSDG2
3-fold upgrade to max 12 fold system
without digital controller RQ-3M5DLOTRDG2E RQ-3M01LOTRDG2E RQ-3C5DLETSDG2E RQ-3C01LETSDG2E
Control Unit
Digital controller
15’’ color display with touch screen • • • •
pH and pO2 calibration routine
Single probe and group calibration • • • •
Control capabilities listing per vessel
Temperature, pH, DO (multi stage cascade),
Stirrer speed; Foam|Harvest, Substrate • • • •
Supply Tower
Gasmixing (integrated) O2-Enrichment Exclusive Flow
Rotameter for Sparger|Overlay [l/min] •|— — •|•
Solenoid Valve for O2-Enrichment • 4-gas mixing
Gasmixing of Air, O2, N2, CO2 —— •
Mass Flow Controller Total Sparger Flow • 0.03 – 1.5 [l/min] 8848605 • 0.003–0.150 [l/min] 8848606
Peristaltic pumps (integrated) 3 per vessel 3 per vessel
Thermostat system for independent
vessel temperature control • •
Tubing, O-Ring (spare set) • •
Supervisory Process Control Software
MFCS | DA for data storage • •
Culture Vessel Listing per Vessel Jacketed UniVessel®
3-fold culture vessel tray • —— • ——
Individual culture vessel stand —— • —— •
Stirrer shaft with Single Mechanical Seal • • • •
6-blade disk impeller 2 2 — —
3-blade segment impeller — — 1 1
3 shared storage bottles 250 mL with tray • — • —
3 storage bottles 250 mL with bottle holder — • — •
Air Inlet and Exhaust filter 2 2 3 3
Aeration tube with Ring-sparger • • — —
Aeration tube with Micro-sparger — — • •
Innoculation|addition kit • • • •
Exhaust Cooler • • • •
Addition fitting 2 + 3-way 4-way 2 + 3-way 4-way
Sample-|Harvest pipe • • • •
Manual sampler • • • •
Temperature probe • • • •
pH Electrode, cable • • • •
DO Electrode, cable • • • •
Level|Foam sensor, cable • • • •

• = included, — = not included, — — = unavailable, • = option

Sales and Service Contacts
For further contacts, visit www.sartorius-stedim.com
Europe
Germany Austria
Sartorius Stedim Biotech GmbH Sartorius Stedim Austria GmbH
August-Spindler-Strasse 11 Franzosengraben 12
37079 Goettingen A-1030 Vienna
Phone +43.1.7965763.18
Phone +49.551.308.0
Fax +43.1.796576344
Fax +49.551.308.3289
www.sartorius-stedim.com
Belgium
Sartorius Stedim Belgium N.V.
Sartorius Stedim Systems GmbH Leuvensesteenweg, 248/B
Schwarzenberger Weg 73–79 1800 Vilvoorde
34212 Melsungen
Phone +32.2.756.06.80
Phone +49.5661.71.3400 Fax +32.2.756.06.81
Fax +49.5661.71.3702
www.sartorius-stedim.com Denmark
Sartorius Stedim Nordic A/S
Hoerskaetten 6D, 1.
France DK-2630 Taastrup
Sartorius Stedim Biotech S.A.
Phone +45.7023.4400
Z.I. des Paluds
Fax +45.4630.4030
Avenue de Jouques – BP 1051
13781 Aubagne Cedex
Italy
Phone +33.442.845600
Fax +33.442.845619 Sartorius Stedim Italy S.p.A.
Via dell’Antella, 76/A
50012 Antella-Bagno a Ripoli (FI)
Sartorius Stedim France SAS Phone +39.055.63.40.41
ZI des Paluds Fax +39.055.63.40.526
Avenue de Jouques – CS 71058
13781 Aubagne Cedex
Netherlands
Phone +33.442.845600 Sartorius Stedim Netherlands B.V.
Fax +33.442.846545 Edisonbaan 24
3439 MN Nieuwegein
Phone +31.30.6025080
Fax +31.30.6025099
Spain
Sartorius Stedim Spain SA
C/Isabel Colbrand 10–12,
Planta 4, Oficina 121
Polígono Industrial de Fuencarral
28050 Madrid
Phone +34.91.3586102
Fax +34.91.3588804
Switzerland
Sartorius Stedim Switzerland GmbH
Lerzenstrasse 21
8953 Dietikon
Phone +41.44.741.05.00
Fax +41.44.741.05.09
U.K.
Sartorius Stedim UK Limited
Longmead Business Park
Blenheim Road, Epsom
Surrey KT19 9 QQ
Phone +44.1372.737159
Fax +44.1372.726171
America Asia |Pacific
USA China
Sartorius Stedim North America Inc. Sartorius Stedim Beijing
131 Heartland Blvd. Representative Office
Edgewood, NY 11717 No. 33, Yu’an Road,
Airport Industrial Zone B, Shunyi District
Toll-Free +1.800.368.7178
Beijing 101300
Fax +1.631.254.4253
Phone +86.10.80426516
Fax +86.10.80426580
Sartorius Stedim SUS Inc.
1910 Mark Court
Concord, CA 94520 Sartorius Stedim Shanghai
Represantative Office
Phone +1.925.689.6650
Room 618, Tower 1, German Centre,
Toll Free +1.800.914.6644
Shanghai, PRC., 201203
Fax +1.925.689.6988
Phone +86.21.28986393
Fax +86.21.28986392.11
Sartorius Stedim Systems Inc.
201 South Ingram Mill Road
Springfield, MO 65802 Sartorius Stedim Guangzhou Office
Room 704, Broadway Plaza,
Phone +1.417.873.9636
No. 233–234 Dong Feng West Road
Fax +1.417.873.9275
Guangzhou 510180
Phone +86.20.8351.7921
Argentina
Fax +86.20.8351.7931
Sartorius Argentina S.A.
Int. A. Avalos 4251
B1605ECS Munro India
Buenos Aires Sartorius Stedim India Pvt. Ltd.
Specifications subject to change without notice. Printed in Germany on paper that has been bleached without any use of chlorine. W/sart-000 · G.
10, 6th Main, 3rd Phase Peenya
Phone +54.11.4721.0505
KIADB Industrial Area
Fax +54.11.4762.2333
Bangalore – 560 058
Phone +91.80.2839.1963|0461
Brazil
Fax +91.80.2839.8262
Sartorius do Brasil Ltda
Av. Dom Pedro I, 241
Bairro Vila Pires Japan
Santo André Sartorius Stedim Japan K.K.
São Paulo KY Building, 8–11
Cep 09110-001 Kita Shinagawa 1-chome
Shinagawa-ku
Phone +55.11.4451.6226
Tokyo 140-0001
Fax +55.11.4451.4369
Phone +81.3.3740.5407
Fax +81.3.3740.5406
Mexico
Sartorius de México S.A. de C.V.
Circuito Circunvalación Poniente No. 149 Malaysia
Ciudad Satélite Sartorius Stedim Malaysia Sdn. Bhd.
53100 Naucalpan, Estado de México Lot L3-E-3B, Enterprise 4
Technology Park Malaysia
Phone +52.5555.62.1102
Bukit Jalil
Fax +52.5555.62.2942
57000 Kuala Lumpur
Phone +60.3.8996.0622
Fax +60.3.8996.0755
Publication No.: SBI2008-e08012 · Order No.: 85030-533-25
Singapore
Sartorius Stedim Singapore Pte. Ltd.
10, Science Park Road, The Alpha
#02-25, Singapore Science Park 2
Singapore 117684
Phone +65.6872.3966
Fax +65.6778.2494
Australia
Sartorius Stedim Australia Pty. Ltd.
Unit 17/104 Ferntree Gully Road
Waverley Business Park
East Oakleigh, Victoria 3166
Phone +61.3.9590.8800
Fax +61.3.9590.8828