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ASSIGNMENT BIOLOGY CLASS - XII GSEB

TOPIC : BIOTECHONOLOGY-PRINCIPLES & PROCESSES

SECTION - A
SINGLE CORRECT QUESTIONS [01 Mark Each]

1. EFB stands for 6. The linking of anitbiotic resistance gene with

a) European Federation of Biotechnology the plasmid vector became possible with
b) Eurasian Federation of Biotechnology a) DNA ligase b) RNA ligase
c) East Asia Federation of Biotechnology c) DNA polymerase d) RNA polymerase
d) Ethiopian Federation of Biotechnology 7. Transfer of any gene into a completely different
organisms can be done through
2. ........ deals with microorganism, plant or animal
cells or their enzymes to produce products and a) Genetic engineering
processes useful to human. The most b) Tissue culture
appropriate choice to fill the blanks is
c) Transformation d) None of these
a) Microbiology b) Biotechnology
8. Which of the following techniques is most
c) Molecular biology commonly used to separate DNA molecules by
d) Biochemistry size ?
3. The structure involved in genetic engineering is a) Chromatography b) PCR
a) Codon b) Anticodon c) RFLP d) Gel electrophoresis
c) Vector d) Plasmid 9. In gel electrophoresis, the separated bands of
DNA are cut and extracted from the gel piece.
4. Which of the following is known as molecular
This step is called
scissors of of DNA ?
a) Elution b) Origin replication
a) Ligase b) Polymerase
c) Competency d) Transformation
c) Restriction endonucleases
10. Which of the following is a plasmid ?
d) Transcriptase
a) pBR322 b) Bam II
5. In recombinant DNA technique, the term vector
refers to a c) Sal I d) Eco RI
a) Donor DNA, it is identified picked up 11. An enzyme catalysing the removal of
through electrophoresis nucleotides from the ends of DNA is
b) Plasmid, transfers DNA into living cell a) Endonuclease b) Exonuclease
c) Collection of entire genome in form of c) DNA ligase d) Hind II
plasmid 12. The given figure is the diagrammatic
d) Enzyme, cuts the DNA at specific sites representation of the E. coli pBR322. Which one
of the given options correctly identifies its
certain components?

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 a) Transformation
b) Transduction
c) Downstream processing
d) Upstream processing
19. Which of the following techniques can be used
to detect genetic disorders in human ?
a) Polymerase Chain Reaction (PCR)
b) Gel electrophoresis
c) Spectroscopy d) All of the above
20. Variable Number of Tandem Repeats (VNTR)
a) ori–original restriction enzymes in the DNA molecule are highly useful in
b) rop–reduced osmotic pressure a) Recombinant DNA technology
c) Hind III, Eco RI–selectable markers b) DNA fingerprinting
d) ampR, tetR–antibiotic resistances genes c) Monoclonal antibody production
13. A single PCR amplification cycle cycle involves d) Stem cell culture
a) Denaturation b) Annealing 21. The mobile genetic element is
c) Extension d) All of these a) Transposons b) Mutation
14. Taq polymerase enzyme used in PCR in isolated c) Endonucleases d) Variation
from
22. Agrobacterium tumefaciens is used n genetic
a) Thermus aquaticus b) Thermococcus litoralis engineering for
c) Salmonella typhimurium a) DNA - mapping
d) None of the above b) DNA - modification
15. Thermostable enzymes ‘taq’ and ‘uent’ isolated c) Cloning vector to deliver genes into host
from thermophilic bacteria are
d) DNA finger printing
a) DNA polymerase
23. Which structure involved in genetic
b) DNA ligase engineering ?
c) Restriction endonucleases a) Plastid b) Plasmid
d) RNA polymerase c) Codon d) None of these
16. Enzyme that is used in PCR technology is 24. Bt toxin is
a) Ligase a) Exotoxin, biodegradable insecticide
b) Polymerase b) Exotoxin nonbiodegradable insecticide
c) Helicase c) Endotoxin nonbiodegradable insecticide
d) Reverse acid synthesis d) Endotoxin biodegradable insecticide
17. Process of formation of RNA from DNA is 25. ti plasmids used in genetic engineering is
called obtained from
a) Transduction b) Transcription a) Bacillus thuringiensis
c) Transformation d) Translation b) Agroba cterium rhizogenes
18. After completion of the biosynthetic stage in the c) Agrobacterium tumefaciens
bioreactors, the product undergoes. Separation
and purification processes, collectively termed d) Pseudomonas syringae
as

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 SECTION - B
Short Answer type Question: [02 Marks Each]
1. What are palindromes? Gives Example.
2. What are sticky ends?
3. What are the two types of gene Library.
4. Describe common characteristics of vectors.
5. Distinguish between retro transposns and DNA transpsons.
6. Write a note on CDNA library.
7. Discuss bio-safety issues.
8. Describe steps that constitute one cycle of reaction in PCR.
9. Explain terms- 1. Denaturation of DNA. 2. Annealing 3. Gene amplification.
10. List the key tools used in recombinant DNA technology.
11. How is DNA isolated from prokaryotic and eukaryotic cells?

SECTION - C
Long Answer type Question: [03 Marks Each]
1. What are exo nuclease and endonuclease.
2. Distinguish Genomic Library and CDNA Library.
3. What is bacteriophage? Describe how they are used as vectors.
4. Sketeh and neat labeled the E.coli Cloning vector pBR322.
5. What is meant by recognition sequences? Write recognition sites of E CORI and where it cuts DNA.
6. What are Bt crops? Explain in brief about how they are produced with a suitable vector.
7. Describe Gene amplification (PCR).
8. Explain how plasmids are used as vector in genetic engineering.
9. Enlist various enzymes used in DNA techonology.
10. Describe in brief transposon, Plasmids and bacteriophages.
11. Describe the steps in r DNA technology.

SECTION - D
Very Long Answer Type Questions : [05 Mark Each]
1. Discuss the various tools of recombinant DNA technology.
2. Name the GM bacterium whose products is used as a clot buster. Name the product. Specify its use in
medical science.
3. With the help of diagrams shows the different steps in the formation of recombinant DNA action of
restriction endonuclease enzyme E CORI.
4. Why DNA cannot pass through the cell membrane? Explain how is bacterial cell made competent to
take up recombinant DNA from the medium.

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