Dream it.

Multiplex it.
Accelerate Your Research with
Customized MILLIPLEX® map Assays
Designed for YOUR Samples

• Develop new analytes

• Multiplex new analytes with
existing analytes
• Rearrange existing analytes into
new panels
• Design and validate assays on
your samples
• Kit bridging for lot-to-lot
consistency

The life science business

of Merck KGaA, Darmstadt,
Germany operates as
MilliporeSigma in the
U.S. and Canada.
 SPRCUS

Develop a new assay

Our customized Special Product Release (SPRCUS) program allows you to develop a new MILLIPLEX® map assay
which is fit for your purpose. We follow a milestone-based approach to generate an assay that quantifies your
exact proteins of interest. All assays are optimized to be run on any Luminex® instrument. A dedicated project
manager coordinates updates and data reviews during each milestone, offering you peace of mind that your
project is on schedule and meeting specifications.

Milestone 1: N
 ew analyte development or testing existing analytes on a new sample type
(as required)
Milestone 2: Multiplexing of analytes and assay verification
Milestone 3: Kit manufacturing and QC range assignment

Client Project Feasibility and Project Workflow

Client:
Submit basic project details
Feasibility

Custom Immunoassay Team:

Check reagent availability, dilution factors,
sample preparation, other constraints

Custom Immunoassay Team:

Issue a written project work plan
Development

Data Data Assay

Assay

Review Review Shipment

Project Milestones
 SPRCUS

New multiplex
assay
development
checklist
Steps Features Checklist
Human
Mouse
Rat
Primate
Porcine
1. Select species Feline
Canine
Equine
Multi-Species (Some Analytes)
Other
Serum Usage
Plasma Usage
2. Choose sample type Cell Culture Supernatants Usage
Other Usage (CSF, Urine, etc.)
Custom Analyte Combinations
New Analytes
3. S
 elect assay
Combine New + Existing or New + New Analytes
development options Assay Development with Customer-Provided Samples
Assay Development with Customer-Provided Reagents
• Screening of Matrices for Dilution of Standards (Minimize background)
• Sample Dilution Factor Provided for Multiplexed Analytes
Sample Extraction Procedures (When Applicable)
Performance Testing of Analytes in Custom Multiplex Format
• Analyte Crossreactivity Testing
4. L
 ist required assay • Optimized Standard Curve With Relevant Assay Range
verification and Custom Assay Validation (In-house Samples)
validation • Custom Assay Validation (Customer-Provided Samples)
Assay Sensitivity Report
Assay Precision Report (Intra- and Inter-Assay)
Spike-Recovery in Samples
Extensive Validation Testing Services
Other
End-User Mixed Beads
Premixed Beads
End-User Mixed Standards (Lyophilized)
Premixed Standards (Lyophilized)
Quality Controls (Low, High) and Ranges
End-User Mixed Detection Cocktails (Hydrated Format)
5. S
 elect desired kit
Customized Detection Cocktail (Hydrated Format)
format Optimized Serum Matrix for Dilution of Standards (Serum/Plasma Samples Only)
Bulk Reagent Packaging
Customized MILLIPLEX® Protocol
Standard MILLIPLEX® reagents, plates/sealers and assay buffers
96-well Format
384-well Format (FlexMap3D Usage Only)
• Minimum Assay Verification (Always Included)

Submit an SPRCUS request at

EMDMillipore.com/customassay
 SPR

Reconfigure existing panels

Do we already have the analytes you are looking for validated for your sample type but
just not in one convenient panel? No problem — we have got you covered!
Our Special Product Release (SPR) program allows you to reconfiguring existing analytes by leveraging
pre-validated standards, beads, antibodies and buffers. Save your samples by reconfigure existing
analytes from three or less MILLIPLEX® map panels into one custom panel. We assess feasibility of analyte
combinations for you based on cross-reactivity, sample dilution and sample preparation.

Example

7-plex Human Cytokine / Metabolic / Sepsis Panel

IL-1β, IL-4, IL-6, IL-8, TNFα, Leptin and MMP-8

Human Cytokine/Chemokine Human Metabolic Hormone Human Sepsis Panel 2

Panel I (Cat. No. HMHEMAG-34K) (Cat. No. HSP2MAG-63K)

+ +
(Cat. No. HCYTOMAG-60K)
Amylin (active) † ◗ IL-6 Granzyme B MIP-1α / CCL3
29 (Cat. No. HCYTMAG-60K-PX29)
Amylin (total) † ◗ Insulin HSP70 MIP-1β / CCL4
29 (Bulk Cat. No. HCYTMAG60PMX29BK)
C-Peptide Leptin IL-1α MMP-8
30 (Cat. No. HCYTMAG-60K-PX30) ♦
Ghrelin (active) ◗ MCP-1/CCL2 IL-8 / CXCL8
30 (Bulk Cat. No. HCYTMAG60PMX30BK) ♦
GIP (total) Pancreatic
38 (Cat. No. HCYTMAG-60K-PX38) Polypeptide (PP)
GLP-1 (active) † ◗
38 (Bulk Cat. No. HCYTMAG60PMX38BK) PYY (total)
GLP-1 (total) † ◗
41 (Cat. No. HCYTMAG-60K-PX41) TNFα
Glucagon ◗
41 (Bulk Cat. No. HCYTMAG60PMX41BK)

sCD40L IL-9
EGF ♦ IL-10 ♦
Eotaxin / CCL11 ♦ IL-12 (p40) ♦
FGF-2 / FGF-basic IL-12 (p70) ♦
Flt3 Ligand IL-13 ♦
Fractalkine / CX3CL1 IL-15 ♦
G-CSF ♦ IL-17A / CTLA8 ♦
GM-CSF ♦ IP-10 / CXCL10 ♦
GRO MCP-1 / CCL2 ♦
IFNα2 ♦ MCP-3 / CCL7
IFNγ ♦ MDC / CCL22
IL-1α ♦
IL-1β ♦
MIP-1α / CCL3 ♦
MIP-1β / CCL4 ♦
Download a copy of
IL-1Ra ♦ PDGF-AA ▲ the new Analyte
IL-2 ♦ PDGF-AB / BB ▲ Quarterly brochure for
IL-3 ♦ RANTES / CCL5 ♦▲
IL-4 ♦ TGFα a complete listing of
IL-5 ♦ TNFα ♦ >600 analytes at
IL-6 ♦
IL-7 ♦
TNFβ / Lymphotoxin-α
(LTA) ♦ EMDMillipore.com/
IL-8 / CXCL8 ♦ VEGF-A ♦ milliplex
Sample
customer
project
Custom-made Multiplex Assays Aid Unique
Toxicity Tests for Bayer Crop Science
Before any agrochemical enters the late research and
development process, it needs to be tested for the
presence of certain hormone-altering effects. When
ingested, these chemicals known as endocrine disruptors,
can cause developmental problems during pregnancy,
abnormal growth patterns, abnormal reproductive
function and also trigger some forms of cancer.
Blanck’s team has a portfolio of hormones that is
measured onsite using multiplex assays. While ‘off
the shelf’ assays can come in handy, Blanck often
customizes these assays in collaboration with the
MilliporeSigma Custom Immunoassay team. “We
wanted them to optimize the kits so they could better
answer our questions,” says Blanck. “The standard Dr. Olivier Blanck and a colleague, Sandrine
curve for one of the hormones in an off-the-shelf Pernot, perform the multiplex assay and
kit was not optimal. It meant we had to dilute our examine data. Image courtesy of Dr. Blanck.
samples, risking inaccurate quantification. The Custom
Immunoassay team did a great job by optimizing MILLIPLEX® map Multi-Species Hormone Magnetic
the kit and working on the standard curve for this Bead Panel — Endocrine Multiplex Assay
hormone. This enabled us to have a range of sensitivity
without having to dilute the samples.”
100,000 Cortisol
“We also had custom assays for four different Estradiol
hormones that weren’t available off-the-shelf,” Blanck Progesterone
Testosterone
continues. “This kit only had three analytes. When we T3
asked if it was possible to have a 4-analyte kit, they 10,000 T4
did it.” One of the customized MILLIPLEX® map kits
used by Blanck’s team measures thyroid hormones
and thyroid stimulating hormone (TSH), while another
MFI

1,000
measures steroid hormones such as estradiol, cortisol,
progesterone, and testosterone.
“These two kits are very important for our
endocrine disruption projects because we need 100
accurate reporting of hormone levels. If you
don’t have the kits that correspond specifically
to your scientific needs, it can get very
challenging,” Blanck admits. “Also, the 10
0.01 0.1 1 10 100 1000
MILLIPLEX® map kits go through extensive
ng/mL
validation and have a strong batch-to-batch
reproducibility.” Overnight standard curve from MSHMAG-21K panel, adapted from this
custom assay project.
Excerpt from Select Science article written by Anita
Ramanathan, February 2018.

To read this entire article, visit

EMDMillipore.com/customassay
Key program
statistics
Smallest plex size: Single-plex
Largest Plex Size: 48-plex
Average Plex Size: 12-plex
Number of New Analytes Developed: >50
Success rate of new analyte development: >98%

Single analyte detection

customized
When your assays require detection of single analytes
only, consider custom assay services for SMCxPro®
and ELISA plate readers. Leverage the sub-picogram
detection capabilities of SMCxPro® to quantify proteins
previously undetectable in samples.
For more information on SMCxPro® visit
EMDMillipore.com/smctech
SMCxPro® ELISA

Visit EMDMillipore.com/customassay for a

complimentary project feasibility assessment

To place an order or receive technical assistance in the U.S. and Canada, call toll-free 1-800-645-5476 MilliporeSigma
For other countries across Europe and the world, please visit: EMDMillipore.com/offices 400 Summit Drive
For Technical Service, please visit: EMDMillipore.com/techservice Burlington, MA 01803

EMDMillipore.com
© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma, the vibrant M,
Millipore, MILLIPLEX and SMCxPro are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All
other trademarks are the property of their respective owners. Detailed information on trademarks is
available via publicly accessible resources.

Lit. No. PB1411EN00 Ver. 2.1

2018-14941
09/2018