Name: ___________________________________ Period: ____________ Date: ______________

2.4 Stickleback Fish Pre Lab Notes 

Working ​SILENTLY​ AND ​INDEPENDENTLY. ​RAISE YOUR HAND IF YOU HAVE A QUESTION
 
Activity #1: Do NOW K-L Chart
K= Know​: L= Learned:
The difference between phenotype 2 things I learned from the ​Where do genes come from?​ v​ideo
and genotype is.. are...

1.__________________________ 1.___________________________________________________
____________________________ ____________________________________________________
____________________________
2. An example is 2.___________________________________________________
____________________________ ____________________________________________________
____________________________

 
Activity #2: Lab Video Directions 
Watch the ​Ms. Paredes' Lab Directions​ and answer the following questions: 

Minute 0:00-5:55
​ hat is today’s ​criteria for success​?​ What 3 things​ do you need to do to earn a 4 today?
1. W
● _________________________________________________________________________________
● _________________________________________________________________________________
● _________________________________________________________________________________
2​. In your own words, summarize the ​4 objectives for today​:
● _________________________________________________________________________________
● _________________________________________________________________________________
● _________________________________________________________________________________
● _________________________________________________________________________________

3. ​What are you going to be looking for in the ​lateral view​?_________________________________

4. What are you going to be looking for in the ​ventral view​? ________________________________

5. You are going to be writing in C for ____________, R for ____________ and A for ___________.

Minute 6:00-8:12
6. What do you need to do if you score ​below a 15/20? ____________________________________

7. ​ What will you do once you score ​above a 15/20? ______________________________________

 Name: ___________________________________ Period: ____________ Date: ______________

8. Once you press the blue gloves to begin Experiment 1, what will we be skipping? What do we need to
click on to skip this part? __________________________________________________________
9. In experiment 1 Part 2, you are going to click on the jar of stained fish that is labeled ___________
_____________.
Minute 9:30-13:41
10. Once you complete the Bear Paw Lake jars, you are going to do the same thing with the jars labeled
___________________ _________________.
11. ​Do marine and sea-run stickleback fish have pelvic structures? ___________
12. What is the claim we will be trying to find evidence for?
_______________________________________________________________________________
Draw an example of the following:
Fish with ​Complete Pelvic Structure Fish with ​Reduced Pelvic Structure Fish with ​Absent Pelvic Structure

Activity #3: Read It​! ​Stickleback Fish Reading By: Michael Bell 
​ ​ ​Freshwater stickleback populations were established when some sea-run CATCH Annotate CFS
populations became trapped in lakes that ❏ Highlight one keypoint from
formed at the end of the last ice age. each paragraph.
These lakes were initially connected to ❏ Circle 1-2 unknown/vocab
the ocean, but were cut off as ice fields words from each paragraph
retreated. ❏ Ask 1 question OR make 1
connection
❏ Answer ​Thinking Questions
In most cases, f​reshwater
stickleback fish​ populations are
1. ​What are pelvic structures?
strikingly ​different from sea-run and
marine fish populations​ because they _______________________________
have adapted to life in freshwater _______________________________
environments. ______________________________
Sea-run and marine stickleback ​fish have strong armor defenses to
2. ​How are sea-run and marine
protect them from large predatory fish that live in the ocean. These ​defenses
include ​complete pelvic structures which are ​bony plates along the sides of stickleback fish different from
their bodies and long spines projecting from their backs and pelvises. Freshwater freshwater stickleback fish?
stickleback fish don’t have such strong armor defenses.
_______________________________

Some ​freshwater stickleback​ populations have reduced armor or none at _______________________________

all. In addition, ​freshwater stickleback​ fish have evolved to be slightly smaller _______________________________
and more streamlined than their sea-run ancestors, allowing them to swim faster _______________________________
and dart more easily into the many hiding places present in lakes. They also have
 Name: ___________________________________ Period: ____________ Date: ______________

modified gill function and an altered excretory system. 3.​ Describe the freshwater
stickleback fish populations.
Although freshwater stickleback fish underwent many changes as they
_______________________________
adapted to life in freshwater, ​researchers have focused on changes in the
pelvic skeleton​ because this trait is highly variable and is easy to see and _______________________________
measure. In addition, its underlying genetic mechanisms are well studied. The _______________________________
development of the pelvic girdle and spines appears to be controlled by a major
4.​Why have researchers focused on
gene called ​Pitx1​. Evidence suggests that the function of this gene has been
conserved throughout vertebrate evolution for millions of years. changes in the pelvic skeleton?
_______________________________
In the ​ocean​, a stickleback fish with a ​complete pelvis​ is ​less likely to be _______________________________
eaten by larger fish that prey on it.​ The pelvic structure allows the fish to defend
______________________________
themselves against the predators. As a result, ​if there are many predator
animals​ in the ocean, the s​tickleback fish will​ most likely ​selecting for and 5.​Do marine and sea-run
pass on the trait for the stickleback fish to have a complete pelvis structure. stickleback fish have pelvic
Almost all​ ​marine​ and ​sea-run ​ stickleback fish have a​ full ​pelvic girdle​ and structures? Why or why not?
pelvic spines​ so they can defend themselves against predators. _______________________________
_______________________________
_____________________________

Activity #4: Phenotype and Genotype Practice  

 
 Name: ___________________________________ Period: ____________ Date: ______________
PHENOTYPES:​Use the following alleles for the traits below.
B = brown eyes D = brown hair G = widow’s peak H = curly hair M = can roll tongue
b = blue eyes d = blonde hair g = no widow’s peak h = straight hair m =cannot roll tongue

Genotype Phenotype

BB GG Brown eyes and Widow’s peak

Bb dd

DD Mm

MM Hh

Bb Hh

Bb Dd Gg

Gg hh mm

Bb Mm DD

BB Dd gg Hh mm

GENOTYPES: ​Use the alleles for the following variations.

B = brown eyes D = brown hair G = widow’s peak H = curly hair M = can roll tongue
b = blue eyes d = blonde hair g = no widow’s peak h = straight hair m =cannot roll tongue
Phenotype List ALL the POSSIBLE genotypes that would match the following phenotypes

Blue eyes, blonde hair, widow’s peak Blue eyes = bb Blonde hair = dd Widow’s peak = GG or Gg
Genotype (2 possibilities) = ​bb dd GG​ ​bb dd Gg

Blue eyes, brown hair, cannot roll tongue

Genotype (2 possibilities) = ​_________________​ ​________________

No widow’s peak, cannot roll tongue, long

arms Genotype (2 possibilities) = ​_________________​ ​________________

Blue eyes, blonde hair, straight hair, cannot

roll tongue, no widow’s peak Genotype (1 possibility) = ​______________​___

Brown hair, blue eyes, widow’s peak

Genotype (4 possibilities) = ​_______​_ ​ ________​ ​_________​ ​_______

Can roll tongue, curly hair, no widow’s peak,

blue eyes Genotype (4 possibilities) = ​_______​_ ​ ________​ ​_________​ ​_______

Brown hair, brown eyes, no widow’s peak

Genotype (4 possibilities) = ​_______​_ ​ ________​ ​_________​ ​_______

Straight hair, blonde hair, brown eyes

Genotype (2 possibilities) = ​_________________​ ​________________

Widow’s peak, blue eyes, straight hair, can

roll tongue Genotype (4 possibilities) = ​_______​_ ​ ________​ ​_________​ ​_______
Name: ___________________________________ Period: ____________ Date: ______________