Abstracts S127
Keywords: Experimental osteoporosis, Sulfurous water, PEMF
Acknowledgements: This study is supported by Grant No 06/06-020/961-49/05
Ministry of Science and Technology Republic of Srpska.
This article is part of a Special Issue entitled ECTS 2012.
Disclosure of interest: None declared.
doi:10.1016/j.bone.2012.02.391
PP203
Interrelationships between PQCT indicators of cortical bone mass,
“quality” and design along the tibiae of healthy sedentary and
trained men and women
R.F. Capozzaa, S. Feldmanb, P.A. Mortarinoa, P.S. Reinaa, L.M. Nocciolinoa,
J. Rittwegerc, J.L. Ferrettia, G.R. Cointrya,⁎
a Disclosure of interest: None declared.
Center for P-CA Studies (CEMFoC), Natl Univ of Rosario, Rosario,
Argentina
b
LABOATEM, Natl Univ of Rosario, Rosario, Argentina
c
German Space Agency (DLR), Cologne, Germany
Abstract: Bone strength is determined exclusively by material and geometric
properties. In support of that concept, we have shown negative associations between
material and geometric bone properties in animal and human studies. In this study,
pQCT indicators of tibia cortical bone tissue mass (cortical BMC), distribution (bending
and torsion moments of inertia, MIs) and “quality” (cortical vBMD, known to reflect
cortical tissue stiffness) were determined in serial scans taken at 5% intervals
throughout the tibia length in 10/12 men/women with sedentary habits and in 10/10
men/women trained in long-distance running (10–15 km/session, 3–5 sessions/wk in
the last 8 years), aged 25–40 yr and with similar body weight and height. In every scan
the MIs values (y) were correlated with cortical BMC (“distribution/mass”, d/m
curves) and vBMD data (“distribution/quality”, d/q curves) for all groups. The d/m and
d/q curves were regarded as describing the architectural efficiency of cortical tissue
distribution (MIs) as a function of its availability (BMC) or its mechanical quality
(vBMD), respectively.
The d/m (positive exponential) and d/q (negative hyperbolic) relationships could
be described by single curves for all groups together at every bone site in all instances.
The generally high significance of the d/m and d/q curves was not homogeneous
along the bones. Their correlation coefficients, plotted by bone site, showed always
bell-shaped distributions, with the highest values concentrated toward the central
diaphyseal region.
Results show that the modeling-dependent adaptation of tibia diaphyseal design
to usage-induced bending or torsion strains proceeds as a function of both the mass
and the degree of mineralization (an indicator of intrinsic stiffness) of the available
cortical tissue, more evidently toward the central region of the tibia, where the
bending and torsion strains are known to assume their highest influence within the
complex pattern of mechanical stimulation of the bone. The existence of unique
equations describing the behavior of data from sedentary and trained men and women
reveals that physical activity counts as a strong, natural determinant of bones'
adaptation to the mechanical environment through changes in geometric properties.
This is congruent with the idea that bones auto-control their structural stiffness
through the generation of modeling drifts as a vectorial function of the mechanical
usage of the skeleton, with a high regional specificity.
This article is part of a Special Issue entitled ECTS 2012.
Disclosure of interest: None declared.
doi:10.1016/j.bone.2012.02.392
PP204
Effects of thalidomide on bone mechanical properties in young
rats
I. Kaczmarczyk-Sedlak⁎, M. Zych
Pharmacognosy and Phytochemistry, Medical University of Silesia,
Sosnowiec, Poland
Abstract: Thalidomide has been approved for the treatment of multiple myeloma and
erythema nodosum leprosum. Thalidomide is currently one of the most intensely studied
drugs because of its multidimensional activity. Clinical trials for the use of thalidomide in
pediatric patients with juvenile rheumatoid arthritis, bowel disease, Crohn's disease, brain
tumors, various neoplastic diseases, chronic graft-versus-host disease, numerous derma-
tological conditions and others have been conducted. However, the effect of thalidomide on
the osseous tissue of children has not been recognized.
The aim of the present study was to examine the effect of thalidomide on bone
mechanical properties in young rats.
The experiments were carried out on 5-week-old male rats, divided into 4 groups: I —
control, II — thalidomide 15 mg/kg po, III — thalidomide 30 mg/kg po, IV — thalidomide
60 mg/kg po. The drug was administered once a day for 6 weeks.
The mechanical properties of the tibial metaphysis (maximal load, displacement
for the maximal load, breaking load, displacement for the breaking load), femoral
diaphysis (maximal load, displacement for the maximal load, breaking load,
displacement for the breaking load) and femoral neck (maximal load) were studied.
Bone mass, bone mineral, calcium and phosphorus content were examined in tibia,
femur and L-4 vertebra.
The effects of thalidomide on the skeletal system of young rats depended on
the dose. Thalidomide given at a dose of 15 mg or 30 mg/kg po did not affect the
investigated parameters. Thalidomide administered at a dose of 60 mg/kg po
decreased the maximal load of the tibial metaphysis, femoral diaphysis and femoral
neck. The mineral and calcium content in bone were also decreased.
Acknowledgement: This study was supported by grant no KNW-1-014/P/1/0 from
Medical University of Silesia.
This article is part of a Special Issue entitled ECTS 2012.
doi:10.1016/j.bone.2012.02.393
PP205
Structural micro-CT study in femurs of type 2 diabetic Goto-Kakizaki
rats without overweight after gastrojejunal bypass. Deleterious
effect of bariatric surgery
J.L. Perez-Castrillona,⁎, J.A. Rianchob, D. De Luisa, J.R. Caeiroc, D.
Gueded, M. Ruiz-Mambrillae, M. Gonzalez-Sagradof, R. Condeg, M.
Domingo-Andresg, D. Primo-Martinf, A. Dueñas-Laitaa
a
Medicine, University of Valladolid, Valladolid, Spain
b
Medicine, University of Cantabria, Santander, Spain
c
Traumatology, University Hospital, Santiago de Compostela, Spain
d
Trabeculae, Ourense, Spain
e
Medical Center of Rehabilitation and Language, Spain
f
Institute of Endocrinology, Spain
g
University Hospital Rio Hortega, Valladolid, Spain
Abstract: Background: The effects of type 2 diabetes on bone mass and
microstructure are not clear. The aim of this study was to evaluate bone
microstructural properties and volumetric bone mineral density (vBMD) in type 2
diabetic Goto-Kakizaki rats without overweight after gastrojejunal bypass, and their
relationship with hormonal parameters. Material and methods: We designed an
experimental study in Goto-Kakizaki rats with and without gastrojejunal bypass,
performing densitometric and microstructural studies of the distal femur using X-ray
computed microtomography (micro-CT). Levels of insulin, glucagon, leptin and
glucagon-like peptide-1 (GLP-1) were also determined. Results: We observed reduced
cortical (1488.92 ± 98.2 mg/cm3 vs 1727.92 ± 133.45 mg/cm3 p = 0.028) and trabecu-
lar (180.8 ± 9 mg/cm3 vs. 261.23 ± 45.54 mg/cm3 p = 0.036) vBMD in operated rats.
Bone volume fraction (BV/TV) and trabecular connectivity were reduced in operated
rats, while there was a reduction in cortical thickness and an increase in rod-like
trabeculae at the expense of plate-like trabeculae. Leptin was reduced (1042 ± 549 vs.
2447 ± 1035 p = 0.05) and GLP-1 increased (1.62 ± 0.32 vs 0.96 ± 0.1 p = 0.008) but
only leptin showed a significant association with vBMD. Conclusion: In type 2 diabetic
Goto-Kakizaki rats, gastrojejunal bypass produces a reduction in cortical and
trabecular bone mineral density and a deterioration in bone quality that may be
explained, in part, by the reduction in leptin levels.
This article is part of a Special Issue entitled ECTS 2012.
Disclosure of interest: None declared.
doi:10.1016/j.bone.2012.02.394
PP206
Bone remodeling after pip joint arthroplasty using pyrocarbon:
A material isoelastic to bone
M. Tägila,⁎, A. Abramoa, M. Geijerb, P. Kopylova
a
Orthopedics, Lund University Hospital, Lund, Sweden
b
Radiology, Lund University Hospital, Lund, Sweden
Abstract: In 2001 the Ascension® Pyrocarbon PIP-prosthesis was introduced.
Pyrocarbon is a form of carbon, durable, tissue compatible and wear-resistant.
Pyrocarbon has an elastic modulus similar to cortical bone, thus minimizing the shear
in the interface and relative motion between the prosthesis and surrounding bone in