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Key points:
There were three models for how organisms
might replicate their DNA: semi-conserva ve,
conserva ve, and dispersive.
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_Image modified from "Basics of DNA replica on: Figure 1," by OpenStax
College, Biology (CC BY 3.0)._
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_Image modified from "Basics of DNA replica on: Figure 2," by OpenStax
College, Biology (CC BY 3.0). Original artwork from "Meselson-Stahl
experiment diagram en," by Mariana Ruiz Villareal (public domain)._
Genera on 0
DNA isolated from cells at the start of the
experiment (“genera on 0,” just before the switch
to 14 N medium) produced a single band a er
centrifuga on. This result made sense because the
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Genera on 1
DNA isolated a er one genera on (one round of
DNA replica on) also produced a single band when
centrifuged. However, this band was higher,
intermediate in density between the heavy 15 N
DNA and the light 14 N DNA.
Genera on 2
Informa on from the second genera on let
Meselson and Stahl determine which of the
remaining models (semi-conserva ve or dispersive)
was actually correct.
_Image modified from "Basics of DNA replica on: Figure 2," by OpenStax
College, Biology (CC BY 3.0). Original artwork from "Meselson-Stahl
experiment diagram en," by Mariana Ruiz Villareal (public domain)._
Conclusion
The experiment done by Meselson and Stahl
demonstrated that DNA replicated semi-
conserva vely, meaning that each strand in a DNA
molecule serves as a template for synthesis of a
new, complementary strand.
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isaacboardman ago
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Ivana - Science trainee ago
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Todor Cvetanovic ago
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Ivana - Science trainee ago
h ps://study.com/academy/lesson/esc
herichia-coli-e-coli-as-a-model-
organism-or-host-cell.html
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the cytoplasm.
h ps://www.intechopen.com/books/-
i-escherichia-coli-i-recent-advances-
on-physiology-pathogenesis-and-
biotechnological-applica ons/-i-
escherichia-coli-i-as-a-model-
organism-and-its-applica on-in-
biotechnology
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Ivana - Science trainee ago
Replica on is semi-conserva ve in
both: Eukaryotes and Prokaryotes
meaning that it is the same for any
living organism.
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