Service Manual For Erma Pce210

PCE-210SME Ver. 5.
03
FULL AUTOMATIC BLOOD CELL COUNTER

PCE-210
Installation Instruction &

Maintenance Service Manual
For safe use of PCE-210, please read this Installation Instruction

Maintenance Service Manual well and understand function &
operation.
Keep this Installation Instruction & Maintenance Service Manual with
you to refer anytime necessary.
PCE-210SME Ver. 5.03
FULL AUTOMATIC BLOOD CELL COUNTER

PCE-210
Installation Instruction & Maintenance Service Manual
Contents
1 SAFETY NOTES TO USERS 1
2 COMPONENTS & FUNCTION OF THE UNIT 3
2-1　FRONT VIEW 3
2-2　REAR VIEW 4
2-3　RIGHT SIDE VIEW 4
2-4　HEMOLYSYS REAGENT HOLDING SPACE 5
2-5　MAINTENANCE SPACE OF LIQUID CIRCUIT 5
3 INSTALLATION 6
3-1　OPENING CARTON BOXES 6

3-2　ACCESSORIES 6
3-3　REQUIREMENTS 7
3-4　INSTALLATION PROCEDURE 8
3-4-1　Connecting power code 8
3-4-2　Connecting reagents 9
3-4-3　Setting print paper 11
3-5　TURNING POWER ON 12
3-6　FILLING OF REAGENTS 12
3-6-1　Filling of diluent 12
3-6-2　Filling of hemolyzer 12
3-6-3　Filling of cleaning solution 12
4 ELECTRIC CIRCUIT 13
4-1　BLOCK DIAGRAM 13
4-2　THE WHOLE WIRING DIAGRAM 14
4-3　LIST OF ELECTRIC CIRCUIT BOARDS 15
C.1
4-4　ADJUSTMENTS OF BOARD 16
4-4-1　Power board 16
4-4-2 Pressure sensor board 17
4-4-3　Printer I/F board 18
4-4-4　Panel switch board 18
4-4-5　CPU board 19
4-4-6　I/O board 20
4-4-7　LCD board 22
4-4-8　Wave board 23
4-4-9　Power switch board 25
4-4-10　Probe board 25
4-4-11　WBC detection board 26
4-4-12　RBC detection board 27
4-4-13　+5V/+12V switching regulator 28
4-4-14　Check list 29
5 LIQUID CIRCUIT 30
5-1　THE WHOLE PIPING DIAGRAM 30

5-2　LIST OF ELECTRIC PARTS FOR LIQUID CIRCUIT 30
5-3　FUNCTION OF LIQUID CIRCUIT 31
6 SUB SELECTION 33
6-1 FACTORY ADJUSTMENT 35

6-1-1 SYRINGE VOLUME 35
6-1-2 SUCTION VOLUME 35
6-1-3 FUCTORY CALIBLATION 36
6-1-4 ACTION TIME 36
6-1-5 MOVEMENT ADJUST 36
6-1-6 DISC. VOLUME 37
6-2 FACTORY CONFIRMATION 38
6-2-1 Hgb UNIT 38
6-2-2 SUCTION PRESSURE 38
6-2-3 WORKING TIMES 39
6-3 BASICAL SETTING 40
6-3-1 DISCRIMINATION OUTPUT 40
6-3-2 CLEANING SOLUTION BOTTLE 40
6-3-3 INITIALIZATION 41
C.2
7 MAINTENANCE 42
7-1　MAINTENANCE FOR LONG TIME STORAGE 42

7-2　CHANGE OF PARTS 42
7-3 CLEANING DETECTOR 44
8 TROUBLE-SHOOTING 45
8-1 ERROR MESSAGE 45

8-2 TROUBLES IN MEASUREMENT 47
8-2-1 Problems and causes 47
8-2-2 Causes and corrective action 50
8-3 TROUBLES IN OPERATION 55
8-3-1 Problems and causes 55
8-3-2 Causes and corrective action 56
9 REFERENCE 57
9-1 MODE SWITCH 57

9-2 NOTE FOR USING EXTERNAL PRINTER 58
9-3 RS-232C OUTPUT 59
9-4 ROM REPLACEMENT 61
9-5 CLOG DETECTION 61
9-6 Measurement Control for Pressure Volumetric Method 62
C.3
1 SAFETY NOTES TO USERS
Be sure to read "Safety Notes to Users" before you use this instrument for proper operation.
Notes and cautions stated in this section are important messages regarding safety
in order to protect probable dangers and/or damages, which might occur to users.
So, please be sure to read carefully.
Symbols and their meaning are as follows.
If treated mistakenly, a danger to cause death or wound is

DANGER
expected to be imminent.
If treated mistakenly, a possibility to cause death or wound
WARNIG
is expected to be imminent.
If treated mistakenly, a danger to cause would and physical
CAUTION
damage is expected to happen.
Prohibited Action
Necessary Action before using
No warranty applied if you
instrument.
do this action.
DANGER
Anyone must not disassemble, repair or modify the unit.
Those conducts may risk fire and electrical shock.
Do not perform any conducts other than 'MAINTENANCE'
as indicated in this User's Guide.
WARNING
Be sure to use Power Unit designated.
The use of Power Unit other than designated here might risk fire and
electrical shock.
Power Unit outlet should be independent.

If and outlet is shared with other electric devices, the outlet area may get
heated extraordinarily and is subject to a cause of fire.
1
WARNING
When unplugged, it should not be pulled with a cable.
Pull a plug out by holding a plug with two fingers, or it may
Cause electrical shock or short to bring about fire.
Not to plug or unplug with wet hands.

It may cause electrical shock.
Not to damage power cords and connecting cables, etc.

Do not step on, twist or pull cords and cables.
Power cords and connecting cables get broken and this may cause an
electrical shock and/or fire.　　　　　　
Not to use damaged power cords and connecting cables, etc.

This may cause an electrical shock and/or fire.
Never use other cords and cables than the designated ones.
Cords and cables with less electrical capacity may cause fire.
Stop operation when in extraordinary motion.

Shut off power and unplug a cord immediately when you feel something
quite wrong with such symptoms as burning smell, strange sound, etc.
Take Ground.
Unless it is done, it may cause an electrical shock when
breakdown and electrical power leaking occur.
CAUTION
Do not use thinner or benzene as it may cause change of
color or shape for external stains of the unit, wipe with
soft cloth or cloth slightly wet. For heavy stains, wipe with
diluted detergent solution or with alcohol.
Do not put foreign matters such as bolts or coins inside
the unit. It may cause breakdown.　　　　　　　　　
Use and operate the unit in accordance with enclosed
"User's Guide"
Handle switch keys of the Touch Panel with hand care.
Rough handling keys and handling them with a tip of sensor driver or ball
2
pointed pen may cause breakdown or damage.
For operation of switches and keys, do it one by one Doing two or more
switches at the same time may cause
breakdown or damage.　　　　　　　　　
2 COMPONENTS & FUNCTION OF THE UNIT
2-1　Front view
1.Touch panel LCD display
Displays measuring results,
messages
and operation status. Menu setting
can be done by touching the panel.
　　
2.Pilot light
While this light is on, power of the unit
is on, even when LCD back light is off.
3.On/Off switch
When the main switch on the back is
in ON position, press the button to
turn the power to the unit.
Shutting off the unit can be
normally done by touch panel.
However, in case of emergency,
this switch also shuts off the unit,
however, cleaning is not to be done.
4.Reagent holder panel

At the time of changing reagents, Hemolyzer bottle, Cleaning solution bottle are to be
changed.
5.Suction probe
By pressing START switch button, the probe absorbs sample.
After suction, the probe draws back in the unit for dilution.
3
6.START switch
The switch starts suction of sample for measurement. The switch works only when suction of
sample is so requested and possible.
7.Printer cover
The cover is to be opened for changing print paper.
8.Paper cutter
This cutter is for print paper use. Paper can be cut by pressing the paper downward or
upward.
9.PRINT/FEED switch
PRINT: When LCD panel displays measured values, press the switch button to print out the
data. Every time the button is pressed, the printer prints out.
FEED: While the button is pressed, print paper keeps coming out.
10.LCD Angle Adjustment

This knob is for adjusting LCD angle in the range of +5° ～ -5°.
2-2　Rear view
　11.RS-232C connector
　　　This is to be connected with PC for
data output.
12.MODE switch
　　　This is to be used for setting Mode in
the unit.
13.Connecter of external printer

　　External Printer is to be connected.
　　
14.Cooling fan
It keeps the temperature in the
unit stable. It operates all the time
while power to the unit is on.
15.Fuse holder
　　　A fuse is held in.
4
16.AC inlet
To be connected with the attached power cable and the other side to be connected to AC
outlet.
17.Earth terminal
In case 3-pin type outlet can not be applied, or 2-pin type outlet has no terminal, take 　Earth
with this terminal.
2-3　Right side view
　18.Water supply joint

　　　To be connected to water supply tube
(Blue mark).
　19.Cleaning solution joint

　　　To be connected to Cleaning solution
tube (Green mark).
20.Connector for waste solution tube &

float sensor
　　　To be connected to a waste solution
tube(Red mark) and a float sensor of
waste solution.
21.Detector maintenance panel

Open the panel when cleaning detector.
22.Liquid circuit maintenance panel

　　　 Open the panel when changing roller
pump tube and/or pinch valve tube.
2-4　Hemolysys reagent holding space
23. Heamolysis reagent nozzle

There is a hemolysis reagent
nozzle with cap in the
holding space for aspirating the
5
Hemolysis reagent.
When the Hemolysis reagent is changed, exchange the
Hemolysis reagent bottle for new one removing the
cap.
2-5　Maintenance space of liquid circuit
24.Roller pump-XB1
This pump is for washing the sample aspiration nozzle.
Change the tubing for rollar pump periodically.
25.Roller pump-XB2
This pump is for aspirating the Diluent.
26.Roller pump-XB3
This pump is for discharging the waste solution.
27.Pinch valve (XB4)

This valve is for changing the discharge from
waste solution tank or counting chamber.
28.Pinch valve (XB5)

This valve is for changing the discharge from
WBC counting chamber or RBC counting chamber.
3 INSTALLATION
6
3-1　Opening carton boxes
・Take PCE-210 unit and its accessories neatly out of cartons.
・By reference to the check list, make sure that no accessories are missing and no damages. If
any, contact your local agent immediately.
・Set PCE-210 unit on a stable working table in an inspection room.（Refer to 3-4 Installation
procedure）
3-2　Accessories
PCE-210 unit contains the following accessories.
No. Descriptions Q’ty

1 Instruction Manual 1
2 Power cable 1
3 Fuse (5A) 2
4 Waste solution bottle with tube & sensor (5L) 1
5 Diluent (5L) 1
6 Cleaning solution (2L) 1
The tubing sets for Diluent & cleaning
7 1
solution
8 Hemolysis reagent 'CYMET ERMA' 500ml 1
9 Printing roll 1
10 Cassettes with tube for Roller pump 3
3-3　Requirements
CAUTION
7
1. Use the unit in the environment where room

temperature is in a range of 10℃～35℃.
Particularly, make sure that room temperature for a
diluent will not go out of 18℃～30℃ range to
secure stable results. In addition, install the unit in a
place 30%～80% so that the unit is not subject to
dew.
2. Install the unit on a flat and sturdy table that is not

subject to vibration.
3. For installation, avoid the place that is subject to

direct sun, high humidity and water-splashing near
a sink etc.
4. Do not put a container containing reagent, water,

etc. on the unit. If the unit is drenched, it may cause
breakdown.
5. Install the unit in a place where no corrosive gas

such as chlorine gas.
6. Do not use the unit in a dusty place, as it is a

precision instrument to measure micro particles.
7. Electricity consumption is approx. 180Ｗ, voltage is +/-10% against the

standard voltage. Do not use the same power outlet that is also used for air-
conditioner, refrigerator, micro-wave cleaning instrument and constant-
temperature bath etc. which generate noise.
Also, keep the unit about 2～4m away from those units.
8. Do not plug in or unplug for the purpose of starting and/or ending operation.
8
3-4　Installation procedure
3-4-1　Connecting power code
DANGER
・ Do not connect ground cable to a plumbing line such as
Gas pipe.
WARNING
・Be sure to take a proper grounding for safety.
CAUTION
・After you have confirmed that On/Off switch on front panel is
in Off position, then connect electric cables and wires.
・Be sure to use an independent power source for the unit.
Particularly, use the other wall outlet for devices that generate
noise.
Put a connector of the attached power

cable into AC outlet on the back
panel, then push a plug at the other
end of the cable in wall outlet.
The end of a plug is equipped with
earth terminal. In case that ground is
not obtained by the terminal, be sure
to apply ground terminal on the rear
panel of the unit for securing
grounding.
3-Pin type outlet 2-Pin type outlet
9
Ta k e a 2 - P i n Use the
plug adaptor power cable
out of the with 2-pin
power cable adaptor and
and put a connect
ground cable
plug into an to an outlet.
outlet.
grounding is
automatically
done.
3-4-2　Connecting reagents
CAUTION
・Be careful not to let contamination in ground reagent.
・Be careful not to let ground tube bend over.
・Try to put a diluent solution bottle on the same height as the unit, as much as
possible.
・Try to put a waste solution bottle on the same height as the unit,
as much as possible.
　 Waste solution bottle 　 Cleaning solution bottle
Dil uent bottle
10
1. Take off rubber cover over a joint on the right side panel.
2. Connection of diluent bottle

Connect a diluent bottle tube to a joint (blue)on the right side panel, then connect the
other end of the tube to the joint of a diluent bottle.
3. Connection of Cleaning solution bottle

Connect the reagent nozzle tube (green) to the joint (green) in the reagent holder inside
Front panel, then connect the other end of the nozzle tube to a Cleaning solution bottle.
4. Connection of waste solution bottle

Connect a waste solution bottle tube to a joint (red)on the right side panel, then connect
the other end of the tube to the joint of a waste solution bottle.
5. Connection of float sensor

Connect float sensor cable to the connector on the right side panel.
6. Connection of Hemolyzer bottle

Connect the reagent nozzle tube (yellow) to the joint (yellow) in the reagent holder
inside Front panel, then connect the other end of the nozzle tube to a Hemolysis
11
reagent bottle.
12
3-4-3　Setting print paper

　　　　　　　　　　　　　　　　　　
1. Pull out the ratch of printer cover toward you while pushing printer cover stopper downward.
Printer cover can be removed.
Paper cutter
　　　　　　Paper discharge
　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Cover stopper
2. Pull printer lever down toward you. Insert the top end of a printer roll into the slot of print
paper insertion.
Paper holding lever
Roll paper holder　　　　　　　　　　　　　　　　　　　　　　Paper insertion
3. After print paper comes out of print head, put printer lever back to its position. When you do
so, make sure that print paper is set straight.
　　　　　　　　　　　　　　　　　　　　　　　　　　　　Paper holding lever
FWD
4. Pull the end of print paper out of print paper outlet, then push the printer cover back in while
making sure that print paper will not get caught. For setting printer cover, put the edge of
down part of printer cover in the notch, then push the upper part in.
13
3-5　Turning power on
Turn power to the unit on.

1. Turn on On/Off switch on the front panel
of the unit. Automatic water-supply
mode is activated and 《 WATER
SUPPLY 》 appears on display. Self-
diagonotic of water-supply and circuit
automatically begins.
2. Upon the completion of automatic

water-supply, 《 DATA DISPLAY 》 appears
on display and the unit is ready to
measure. If abnormalities are found at self-
diagnosis, message appears on display.
3-6　Filling of reagents
 On the occasion of installation of the unit and/or change and/or refill of

reagent, be sure to take this operation. Unless this operation is taken,
measurement results may not be obtained correctly.
 Before you start operation, confirm that diluent bottle is full of diluent, cleaning
solution bottle is full of cleaning solution, and Hemolysis reagent bottle is full of
CYMET ERMA respectively, then waste solution bottle is empty.
3-6-1　Filling of diluent
Touch EXTEND key to access to

EXTENTION menu screen, then touch
WATER key.
3-6-2　Filling of hemolyzer
Touch EXTEND key to access to

EXTENTION menu screen, then touch
HEMOLYZER EXCHANGE key.
3-6-3　Filling of cleaning solution
Touch EXTEND key to access to EXTENTION menu screen, then touch WASH key.
14
Touch RETURN key after finishing all operation to close EXTENTION menu.
15
4 Electric Circuit
4-1　Block Diagram
16
4-2　The Whole wiring Diagram
17
4-3　List of electric circuit boards
No. Board name Function

1 PC-1901B　Power　Board Generation & supply of power
2 PC-1802A　Press　Board Pressure sensor
3 N02-001C Printer I/F Board Printer I/F
4 PC-1904　Panel　Board Panel switch
5 PC-1905C　CPU　Board Control over the unit
6 PC-1906B　I/O　Board Drive sensor I/F
7 PC-1907C　LCD　Board LCD & Touch panel I/F
8 PC-1908B　WAVE　Board Signal management
9 PC-1910　Power SW　Board Power switch
10 PC-1911　Probe　Board Probe drive I/F
11 NC-8005C(W) Detection of WBC signal
12 NC-8005C(R) Detection of RBC signal
13 SNP-Z073 +5V/+12V switching regulator
18
4-4　Adjustments of board
4-4-1　Power board
This board supplies +5V and +12V output of each switching regulator to each circuit
board. In addition, it generates +100V, and +/-15V voltage from +12V switching
regulator.
Name of board : PC-1901B
Adjustment : To be done after switch regulator adjustment.

Set Measuring point Setting value
VR1 TP9 (+100V) Dial knob for voltage adjustment
TP10 (GND.B) To be set at +100.05V+/- 0.05V.
VR2 TP6,8 (+/-15V) Dial knob for voltage adjustment
TP7 (GND.A) To be set at +15.05V+/- 0.05V.
Check Point
Check point Check voltage
TP1(+5V)-TP2(GND) Digital 5V +5.0+/-0.1V
TP3(+12V)-TP4(GND.P) Drive 12V +12.0+/-0.5V
TP6(+15V)-TP7(GND.A) Analog +15V +15.05+/-0.05V
TP8(-15V)-TP7(GND.A) Analog -15V -15.05+/-0.05V
TP9(+100V)-TP10(GND.B) For detect 100V +100.5+/-0.5V
19
4-4-2 Pressure sensor board
This board detects suction pressure, then transduce to voltage.
Name of board : PC-1802A
Adjustment : Adjustment must be done in the way that the board is set in the unit, due to
the influence of power-supply voltage.
Set Measuring point Procedure of adjustment & setting value
VR1 TP1 1. Turn VR1, VR2 to right as far as it can go.
VR2 TP2(GND.A) 2. Disconnect PS1 tube of pressure sensor, then,
make TP1-voltage least with VR2.
3. Suck in PS1 with an injector at -150mmHg, make
TP1-voltage 4.00+/-0.05V least with VR1
4. Repeat 2and3, then check and confirm voltage.
5. Take then injector out of PS1 and confirm min.
voltage of TP1 remains unchanged.
*If changed, repeat 4.
6. Suck in PS1 with an injector at -150mmHg, confirm
voltage of TP1 is 4 +/- 0.005V.
*If changed, repeat 4.
7. Suck in PS1 with an injector at -30mmHg, confirm
voltage of TP1 is 800 +/- 40mV.
*If out of range, repeat 4.
Check point
TP1-TP2(GND.A) Pressure signal：4.00+/-0.05V
*When at -150mmHg
20
-150mmHg ≒ -20kPa
4-4-3　Printer I/F board
This board converts data from CPU board and get printer to print.
Name of board : N02-001C
Adjustment : No items that need to be adjusted.
Check point : No points that need to be checked.
4-4-4　Panel switch board
When panel switch is pressed, this board get printing and feeding started.
Name of board : PC-1904
Check point
SW1 (FEED Switch) To feed paper
SW2 (PRINT Switch) To start printing
21
4-4-5　CPU board
This board activates program and controls over the unit.
Name of board : PC-1905C
Check point
J1(Motion mode) Insert a jump socket in between 1Pin & 2Pin.
*When Flash ROM program is rewritten
insert it in between 2Pin & 3Pin.
J2,J3(applied ROM) Insert a jump socket in between 1Pin &
22
2Pin.（Flash ROM mode）

*When UV-EP ROM is used, insert it in
between 2Pin & 3Pin.
J4,J5(Optional function) Insert a jump socket in between 1Pin &
2Pin. (Standard specification)
TP1(+5V)-TP2(GND) LOGIC 5V +5.00+/-0.20V
TP3(+5V)-TP4(GND.P) PRINTER 5V +5.00+/-0.20V
4-4-6　I/O board
This board controls liquid circuit parts such as electric valves, pumps, motors and driving
unit according to signals from CPU.
Adjustment : Adjust this board after aging for 30min. from power ON.
Set Measuring point Adjustment method & setting value
VR9 TP9 Sample syringe pulse width adjustment
TP2(GND) Adjust TP9 pulse width to 6.0+/-2.5msec
(1 cycle) with VR9.
23
VR10 TP10 Diluent syringe pulse width adjustment

(1 cycle) with VR10.
VR11 TP11 Hemolyzer syringe pulse width adjustment
(1 cycle) with VR11.
Sample syringe pulse
1 ms/div
1 v/div
6.0 msec
Diluent syringe pulse
2 ms/div
1 v/div
12.0 msec
Hemolyzer syringe pulse
24
1 ms/div
1 v/div
6.0 msec
Check point
TP1(+5V)-TP2(GND) For logic 5V 5.00+/-0.20V
TP3(+12V)-TP4(GND.D) For drive 12V 12.00+/-0.20V
TP9-TP2(GND) Sample syringe pulse 6.0+/-2.5msec
TP10-TP2(GND) Diluent syringe pulse 12.0+/-2.5msec
TP11-TP2(GND) Hemolyzer syringe pulse 6.0+/-2.5msec
*Not in use for TP5-TP8
4-4-7　LCD board
This board converts data from CPU to LCD signals, making letters and graphics appear
on LCD. It also transfers coordinates data of touch panel to CPU board.
25
Adjustment
Menu ｰ Eye inspection at Adjustment of LCD contrast
touch panel. To adjust by Menu operation on touch panel LCD.
4-4-8　Wave board
This board selects the only wave that pass through a blood cell from signal wave amplified
by WBC, RBC detection boards, and counts number of a blood cell by size by way of A/D
Transfer and DSP Treatment. It also amplifies HGB detection signal.
26
Adjustment : *Adjust the Hgb voltage with metal cover.

VR1 TP3 PLT Standard voltage-2 adjustment knob
TP9(GND.A) Adjust voltage between TP3-TP9 to
4.15+/-0.01V.
VR2 TP5 PLT Standard voltage-1 adjustment knob
0.80+/-0.02V.
VR3 TP6 RBC Standard voltage-1 adjustment knob
0.40+/-0.02V.
VR4 TP7 WBC Standard voltage-1 adjustment knob
0.75+/-0.02V.
VR5 TP11 HGB Off-set adjustment knob
TP16(GND.A) Disconnect JP7 and adjust voltage between
TP11-TP16 to 0.00+/-0.01V.
VR6 TP13 HGB sensitivity adjustment knob
27
TP16(GND.A) Connect JP7 and adjust voltage (at measuring

diluent) between TP13-TP16 to 4.00+/-0.02V
VR ７ TP33 ADC offset volume for adjustment
TP35(GND.A) Adjust voltage between TP33-TP35 to0.00+/-0.02V
Check point
J1,J2 (Setting of Hgb gain) HGB Gain setting:
Insert a jump socket in between 2 - 3Pin.
J3 (Selection of reset signal ) Reset signal Select:
J4 (Selection of reset signal ) Reset signal Select:
TP2(+5V)-TP4(GND) For logic 5V：5.00+/-0.20V
TP10(+15V)-TP12(GND.A) For analog +15V：+15.00+/-0.20V
TP14(-15V)-TP12(GND.A) For analog –15V：-15.00+/-0.20V
TP3-TP9(GND.A) PLT Standard Voltage2：4.15+/-0.01V
TP5-TP9(GND.A) PLT Standard Voltage1：0.80+/-0.02V
TP6-TP9(GND.A) RBC Standard Voltage1：0.40+/-0.02V
TP7-TP9(GND.A) WBC Standard Voltage1：0.75+/-0.02V
TP8-TP9(GND.A) WBC Standard Voltage2：8.30+/-0.02V
TP17-TP12(GND.A) WBC detection board output wave
TP18-TP12(GND.A) WBC differential wave
TP19-TP12(GND.A) WBC A/D request pulse
TP20-TP12(GND.A) WBC A/D reset pulse
TP34-TP35(GND.A) WBC P/H circuit wave
TP21-TP12(GND.A) RBC detection board output wave
TP22-TP12(GND.A) WBC differential wave
TP23-TP12(GND.A) RBC A/D request pulse
TP24-TP12(GND.A) RBC A/D reset pulse
TP32-TP35(GND.A) RBC P/H circuit wave
TP25-TP12(GND.A) PLT detection board output wave
TP26-TP12(GND.A) PLT differential wave 1
TP27-TP12(GND.A) PLT differential wave 2
TP28-TP12(GND.A) PLT forming wave
TP29-TP12(GND.A) PLT A/D request pulse
TP30-TP12(GND.A) PLT A/D reset pulse
TP31-TP35(GND.A) PLT P/H circuit wave
TP11-TP16(GND.A) HGB offset voltage
TP13-TP16(GND.A) HGB detection voltage
TP1-TP16(GND.A) Pressure detection voltage
28
4-4-9　Power switch board
By pressing the switch, power is turned on to the unit. For turning power to the unit off, it
can be done by touch panel LCD menu operation. However, in case of emergency, you can
shut off the unit by pressing the switch.

Check point :No points that need to be checked.
4-4-10　Probe board
This board drives UP/DOWN motor by control from I/O board. 　It detects location of probe
block and transfers the message to I/O board.
29

4-4-11　WBC detection board
This board flows detection electric current to WBC detection electrode. It amplifies a signal that
was detected at WBC detection part.
Name of board : NC-8005C(W)
Adjustment : VR2 is mounted in the reverse side of the board.

VR2 TP2 WBC Sensitivity adjustment knob
TP3(GND.A) At the time of measuring blank, confirm
that base allowance of signal is within 0.2V.
1. Measure latex particle which you know the exact
volume.
2. Adjust the peak of WBC histogram by VR2 to be
same volume as latex you measured.
JP Short pin for shifting detection electric current
Short B position (center).
30
Check point
TP2-TP3(GND.A) WBC amplification circuit output wave
TP4-TP3(GND.A) WBC 1st stage output wave
4-4-12　RBC detection board
This board flows detection electric current to RBC detection electrode. It amplifies a signal that
was detected at RBC detection part.
Name of board : NC-8005C(R)
Adjustment : VR1 and VR2 are mounted in the reverse side of the board.
VR1 TP1 PLT sensitivity adjustment knob
TP3(GND.A) Measure control blood and arrange in the
way that MPV is within assay value.
Also, at the time of measuring blank, confirm
31
VR2 TP2 RBC sensitivity adjustment knob

TP3(GND.A) Measure control blood and arrange in the
way that MCV is within assay value.
Also, at the time of measuring blank, confirm
JP Short pin for shifting detection electric current
Short B position (center).
Check point
TP1－TP3(GND.A) PLT amplification circuit output wave
TP2－TP3(GND.A) RBC amplification circuit output wave
TP4－TP3(GND.A) RBC 1st stage output wave
4-4-13　+5V/+12V switching regulator
This board generates DC＋5V/+12V from AC power source.
Name of board：SNP-Z073
Adjustment : Switching regulator has been adjusted before shipment.
Check point
P1,2-P3,4,5,6(DC output connector TB2) DC output volgate 5.0+/-0.1V
P7,8-P3,4,5,6 (DC output connector TB2) DC output volgate 12.0+/-0.5V
32
4-4-14　Check list
If you replace the power supply or the boards, please check voltage of each part
according to the following check list.
Name of Product: PCE-210

Serial No.:_____________
ROM Ver.
Check Item Content
Digital 5V+/-0.1 TP1: ________V
Drive12V+ /-0.5 TP3 : ________V

Power Board
Analog+15.05V+/-0.05 TP6: ________V(VR2)
PC-19 01B
Analog-15.05V+/-0.05 TP8: ________V(VR2)
Detector 100.5V+/-0.5 TP9: ________V(VR3)
Pressure Sensor T P 1 ( 0 mm Hg ): __________mV(VR2)

Board PC1802A TP1 (-150mmHg)4V+/-0.05: __________V(VR1)
Sample Syringe 6.0+/-2.5msec TP9 : _________msec(VR9)

I/O Board
Diluent Syringe 12.0+/-2.5msec TP10: _________msec(VR10)
PC1906B
Lyse Syringe 6.0+/-2.5msec TP11: _________msec(VR11)
4.15V+/-0.01 TP3: _______V(VR1)
0.80V+/-0.02 TP5: _______V(VR2)
0.40V+/-0.02 TP6: _______V(VR3)

Wave Board
0.75V+/-0.02 TP7: _______V(VR4)
PC1908B
0.00V+/-0.01 TP11: _______mV(VR5)
4.00V+/-0.02 TP13: _______V(VR6)
0.00V+/-0.02 TP33: _______mV (VR7)
WBC Board Adjust the peak of WBC histogram by VR2 to be same volume as latex particle.
NC-8005C Wave of base signal during blank measurement: _______V
33
*MPV value of control blood is within the range of assay value.(VR1)

RBC Board
*MCV value pf control blood is within the range of assay value.(VR2)
NC-8005C
Wave of base signal during blank measurement: _______ V
Time to reach the aspiration pressure

Aspiration pressure
Pressure Data: _______mmHg
action
Time to reach: _______msec
Hgb unit action A/D value of blank data: _______
5 Liquid Circuit
5-1　The Whole piping Diagram
5-2　List of electric parts for liquid circuit
Parts Application Type Name of parts

XA1 For WBC orifice discharge 2way valve LVM11R-6 DC12V
XA2 For RBC orifice discharge 2way valve LVM11R-6 DC12V
XA3 For WBC orifice supply 2way valve LVM11R-6 DC12V
XA4 For RBC orifice supply 2way valve LVM11R-6 DC12V
XA5 For WBC cell agitation 2way valve LVM11R-6 DC12V
34
XA6 For RBC cell agitation 2way valve LVM11R-6 DC12V

XB1 For suction probe cleaning Roll pump SR10/50-N3.2 DC12V
XB2 For water supply Roll pump SR10/50-N3.2 DC12V
XB3 For waste solution Roll pump SR10/50-N3.2 DC12V
XB4 For waste tank/cell discharge. Pinch valve HYN-5 DC12V
XB5 For WBC/RBC cell discharge. Pinch valve HYN-5 DC12V
XB6 For increasing pressure Air pump CM-15-12
XB7 - " - (Waste tank) 2way valve LVM11R-6 DC12V
YA1 Dilution syringe IN/OUT 3way valve LVM112-6 DC12V
YA2 Hemolyzer syringe IN/OUT 3way valve LVM112-6 DC12V
YA3 Switch for supply & cleaning 3way valve LVM112-6 DC12V
5-3　Function of liquid circuit
XB1 pump and syringe
XB2 pump
35
XB3 pump
XB6 pump
36
6 SUB SELECTION
In this unit, special operations that are not normally in use are separate as SUB SELEC TION,
and it does not appear normally. For having SUB SELECTION menu appear, the special
operation is needed.　Please do either METHOD A or METHOD B as mentioned below.
METHOD A.
Press the clock display 3 times in stand-by screen.
METHOD B.
Put 1 Switch of MODE SWITCH on the back panel
in ON position, then turn the power to the unit on.
Press MENU key to display SELECT MENU.

37
“8 SUB SELECTION”, which is not shown in normal mode, appears.
If you press PRINT button while SUB SELECTION is

displayed, set values are printed by the internal
printer.
 CALBRATION / INSTRUCTION MANUAL 5-1
 BRIGHT ADJUST / INSTRUCTION MANUAL 5-3-6

 PRINTING MODE / INSTRUCTION MANUAL 5-4-1
 OUTPUT SETTING / INSTRUCTION MANUAL 5-4-3
 WBC MODE / INSTRUCTION MANUAL 5-4-4
 SET AUTO CLEANING / INSTRUCTION MANUAL 5-4-6
38
 NORMAL RANGE / INSTRUCTION MANUAL 5-5
 GAIN / INSTRUCTION MANUAL 5-6

 6-1-1 SYRINGE VOLUME
 6-1-2 SUCTION VOLUME

 6-1-3 FACTORY CALIBRATION
 6-1-4 ACTION TIME

 6-3-1 DISCRIMINATION OUTPUT
 6-1-6 DISC. VOLUME
When operations in SUB SELECTION finish:

If you used METHOD A to display SUB SELECTION screen, return to stand-by screen and
press the clock 3 times. If you used METHOD B to display SUB SELCTION screen, turn
DIP switch #1 OFF and turn the power off and on again.
6-1 FACTORY ADJUSTMENT
6-1-1 SYRINGE VOLUME
39
You may change each syringe volume by adjusting

pulse of the syringe pulse moter. PLEASE NOTE
THAT IT MAY AFFECT DATA IN CASE YOU
CHANGE PULSE BECAUSE DILUTION RATIO IS
ALSO AFFECTED.
SAMPLE SYRINGE Approx. 0.55ul/1pulse

DILUENT SYRINGE Approx. 35.53ul/1pulse
HEMORYZER SYRINGE Approx. 6.80ul/1pulse
The input range of each syringe is as below.

SAMPLE SYRINGE 10 - 70
DILUENT SYRINGE 100 - 130
HEMORYZER SYRINGE 40 - 150
6-1-2 SUCTION VOLUME
You can adjust suction time for diluted samples. If

you set the time longer, the suction volume become
larger and accordingly data appears higher. PCE-210
is initially set the time to aspirate 0.25ml each for
WBC and RBC. The input range is as below:
WBC SUCTION VOLUME 6.0 - 15.0
RBC SUCTION VOLUME 8.0 - 16.0
6-1-3 FUCTORY CALIBLATION
You can manually input calibration values in case

measurement data of control blood is out of range
from expected values etc. If you want to change the
calibration values in this MENU, firstly reset the
values set by user to 100%. The input range of
calibration values is 25.0 –200.0% for each
40
parameter.
6-1-4 ACTION TIME
 MEASUREMENT START TIME

Time from the end of stirring in WBC cell to the start
of counting during measurement. Initial value 0.
Setting range 0-1000.
 PROVE WASHING TIME
Time of filling diluent solution to the nozzle washing
block during nozzle cleaning. Initial value 2000.
Setting range 1000-3000.
 STIRRING TIME
Time of stirring sample during measurement. It
indicate time of air venting from the bottom of the
measurement cell. Initial value 400. Setting range
200-1000.
6-1-5 MOVEMENT ADJUST
Just after making operation by using these menus,

there may be change in volume of diluent solution
inside the measurement cell or in volume of solution
on the tip of sample nozzle. Therefore, make sure
either to take blank measurement or to operate water
supply before starting sample measurement.
 PROBE UP/DOWN ACTION

The sample nozzle (probe) moves up and down one time.
 SAMPLE SYRINGE
The sample syringe moves up and down one time.
 DILUENT SYRINGE
The diluent syringe moves up and down one time. Be aware of the diluent solution
discharged from the sample nozzle.
 HEMOLYZER SYRINGE
41
The lysing syringe moves up and down one time. The lysing solution is discharged to the
WBC cell by this operation.
 PROBE ACTION
A continuous sample nozzle action just as same as the action during measurement is
operated. It stops by pressing the key again when the nozzle is in the front.
6-1-6 DISC. VOLUME
Input range of setting value is between 1 and 200.
The unit mentioned as “ad” in screen stands for 1bit

volume of installed in AD converter of this device.
 Clogging Detection Function

In every measurement, this function observes fluctuation of measured voltage between
electrodes of the detection part. When the device detect clogging,, its automatic clog
removing function works and repeats measurement. If the clogging is removed within 2
times of re-measurement, it normally shows the measurement data in display. However, if
the clogging continues, “Clogging Message” will appear after finishing measurement.
 The following is a note for setting value.

The initial set value is for detecting even a tiny clogged situation that actually does not affect
quantity of blood cell but may affect cell volume slightly. If you require higher accuracy in WBC
distribution, Hct, MCV, and MPV, please use the device with the initial set value. To make the
device less sensitive for clogging, please set the bigger value (Around 50). However, please be
noted there may be a little lack of accuracy in cell volume related parameters, if you set the
value larger.
42
6-2 FACTORY CONFIRMATION
6-2-1 Hgb UNIT
Voltage of the Hgb Measurement unit (Value of AD

converter) is displayed. The standard value is 820 +/-
20. 4.0V equals to 820. Please refer to the service
manual for details.
6-2-2 SUCTION PRESSURE
It shows pressure inside the negative pressure tank in

the liquid circuit. It normally takes about 2 sec to
reach –150mmHg. If it takes extremely longer time to
reach –150mmHg, the pump may be deteriorated. Or
if you see the pressure starts decreasing immediately
after reaching the certain point, there may be a
leakage somewhere in the liquid circuit.
43
-150mmHg　　　　　　　　　　　　　　　　　　　　　　　Normal
Deteriorate of Pump tube
　　　　　　　　　　　　　　　　　　　　　　　　　　　　Leakage
　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
0 2000msec t
The following drawing show area which suction pressure reaches or affects ,when you operate
"SUCTION PRESSURE" check.
44
6-2-3 WORKING TIMES
Counters in this screen show the number of each

performance operated so far. By those counters, you
can check how many times the instrument has been
working. The number of the counters can not be
manually changed but you can reset to 0. Press the
item you wish to reset. After the display color is
reversed, press CLEAR key. The countable range for
each item is 0-99999.
6-3 BASICAL SETTING
6-3-1 DISCRIMINATION OUTPUT
If you make the discrimination output “ON”, border

lines showing discrimination position appear in
45
histograms(WBC, RBC, and PLT). Those lines also appear in print-outs by the internal printer but not
in print-outs by the external printer nor in external output.
6-3-2 CLEANING SOLUTION BOTTLE
You can select volume of cleaning solution bottles.

Make sure to set new
cleaning solution after making change in the
selection.
46
6-3-3 INITIALIZATION
If you press “YES” at this menu, all the stored

sample data, input header, calibration values, normal
range, and all the set values in SUB SELECTION
menu are initialized. Also, counters of WORKING
TIMES are reset. Please make sure to take all the
necessary data before if you wish to use this menu.
47
7 MAINTENANCE
7-1　Maintenance for long time storage
CAUTION
Instrument may be badly damaged if you store it for long period
without disconnecting reagents.
If you keep PCE-210 inoperated for long time, remaining diluent solution may
crystallize or contaminate in internal parts of the instrument and could be a cause
of clogging. To avoid any defect in the device, please refer to “5-3-4 Long time
storage” in Operation Manual and refill distilled water to clean the liquid circuit..
Make sure to drain completely after it.
7-2　Change of parts
CAUTION
・ Replace or clean roller pump tube once per 6 months or 3000
measurement, whichever comes first.
・ Make sure to use roller pump tube supplied by manufacturer .
1. At READY SCREEN display, touch MENU key

to get access to SELECT MENU display.
At SELECT MENU screen,
touch MAINTENANCE key to
get access to
CHANGE TUBING display.
2. Touch STEP 1 key. Liquid in the unit is

drained, After liquid has been drained, open liquid circuit maintenance panel on the right side
of the unit, then disconnect a joint which is connected with a roller pump tube.
48
3. Push both notches in horizontal position in with two fingers and pull the roller cassette the
forward you.
Push both notches in the back in

vertical position (90°angle in
comparison with the notches in
above 3.) in with two fingers, then
take out cassette cover and take out
a tube.
4. Push a tube deeply into the inside cassette, check and see if the tube has been firmly held
in, then put a cassette cover back in. After you have heard the sound of check the cassette
had been surely set, then connect the tube to a joint.
5. Touch STEP 2 key. Conform that a pump works.

6. Touch STEP 3 key. Water supply starts. Make sure of no leakage, then close side panel.
49
50
7-3 Cleaning Detector
Clean the measurement related part by following process, when frequent clogging or
unstable
Hgb value is seen.
1. Display stand-by screen and touch

EXTEND key.
2. Open the reagent door in the right side of instrument, pour 5-6 drops of hypochlorite for
both WBC & RBC measurement cell.
CAUTION
・ Please use 5-6% of hypochlorite. DO NOT use home-use
detergent nor breech, which may cause trouble in analyzer.
・ Please do not leave the device after pouring hypochlorite and
proceed to No. 3 below.
3. Close the reagent door and touch

WASH key.
CAUTION
Make sure to touch WASH key
after closing the reagent door.
4. Go back to stand-by screen by touching RETURN key after completing all operation.
51
8 TROUBLE-SHOOTING
8-1 Error message
By way of the self-diagnostic system of the

unit, if there are some troubles, error
messages appear on LCD display. Treat
properly in accordance with the guidance
against each error message.
MESSAGE CAUSE TREATMENT

STORED SAMPLES ERROR Touch CONFIRM key to delete
Stored samples now 200. Memory is over message. Delete stored sample data
Delete Data. 200. since total number of data that can be
stored is 200.
WASTE SOLUTION CHECK Dispose of waste solution in waste
Waste solution full. Waste solution in
solution bottle, then touch
Discard it. the tank is full.
CONFIRM key.
NO PAPER
Print paper is not Set print paper and touch
No paper in printer.
set. CONFIRM key.
Supply paper.
PRINTER ERROR Head-up lever of
Pull down the lever, then touch
No right action. the printer is in up
CONFIRM key.
Check printer action. position.
CREANING SOLUTION CHECK Replace old cleaning solution bottle
Cleaning solution
Cleaning solution short. with new one, then touch
is running short.
Add cleaning solution. CONFIRM key.
HEMOLYSYS REAGENT CHECK Replace old hemolyzer bottle with
Hemolyzer is
Hemolysys reagent short. new one and touch CONFIRM
running short.
Add hemolysys reagent. key.
CHECK PROBE UP/DOWN The probe is not Touch CONFIRM key to delete
52
No right action. properly

Check probe action. performing up and message.
down operation.
CHECK PROBE FRONT/REAR The probe is not
No right action. properly
Touch CONFIRM key to delete
Check probe action. performing front
message.
and rear
operation.
MESSAGE CAUSE TREATMENT

CHECK SAMPLE SYRINGE The sample
No right action. syringe is not Touch CONFIRM key to delete
Check syringe action. properly message.
performing.
CHECK DILUENT SYRINGE The diluent
performing.
CHECK HEMO. REAGENT SYRINGE The hemolyzer
performing.
CHECK WBC CLOGGING Touch CONFIRM key to delete
Measuring part clogged. WBC detector has message. Take cleaning action and
Remove clogging. got clogged. check the remaining volume of
hemolyzer.
CHECK RBC CLOGGING
RBC detector has Touch CONFIRM key to delete
Measuring part clogged.
got clogged. message. Take cleaning action.
Remove clogging.
DILUENT CHECK Refill diluent and check if any tube
Diluent is not full
Diluent not full. bending etc. then touch CONFIRM
in Liquid circuit.
Add diluent key.
CHECK WBC LEAK Suction pressure
in WBC Liquid Check samples and also check and
Sample suction pressure
circuit does not see if any tube bending etc. then,
Not increasing.
reach the fixed touch CONFIRM key.
Check sample.
level.
CHECK RBC LEAK Suction pressure Check samples and also check and
53
in RBC Liquid
Sample suction pressure
circuit does not see if any tube bending etc. then,
Not increasing.
reach the fixed touch CONFIRM key.
Check sample.
level.
INPUT ERROR Abnormality for Touch CONFIRM key. Take
Set values out of range. set values at initialization process for the unit, then
Input values again. memory in the input data again.
unit is found.
CHECK PINCH VALVE Touch CONFIRM key. Please check
Drainage is
Drainage is abnormal. if there are no adhesions in tubes of
abnormal.
Check tube adhesion. XB4 and XB4 pinch valves.
8-2 Troubles in Measurement
8-2-1 Problems and causes
PROBLEMS CAUSES Ref

Blank count can not be “0”. Shortage/Poor quality of Diluent A-1
Poor quality of Hemolyzer A-3
Defect of Water supply circuit A-4
Stains/bubbles of Cell A-8
Clogging/stains/bubbles of Orifice A-9
Stains/bubbles of Electrode A-11
Poor setting of Electrode A-12
Stains/bubbles of probe cleaning A-13
Bubbles when diluent discharge A-22
Bubbles in Water supply circuit A-23
Air leakage of Detection part A-43
Influence by inner noise A-45
Influence by outer noise A-46
Defect of connection cable A-47
Blank count can be “0”, but Clogging/stains/bubbles of Orifice A-9
measured value is high. Defect of Orifice A-10
Position of probe clean A-14
Defect of suction volume A-16
54
Mis-calibration A-17
Defect of WBC sensitivity adjust A-19
Defect of RBC sensitivity adjust A-20
Defect of PLT sensitivity adjust A-21
Bubbles in Hemolyzer circuit A-24
Poor agitation A-25
Defect of Hemolytic time A-26
Defect of Hemolyzer volume A-27
Defect of Sample A-31
Defect of Sample syringe volume A-33
Defect of Diluent syringe motion A-34
Defect of Sample syringe motion A-35
Defect of Hemolyzer syr. motion A-36
Bent/clogging of wiring tubes A-39
Measured value is low. Clogging/stains/bubbles of Orifice A-9
Defect of Orifice A-10
Defect of suction volume A-16
Mis-calibration A-17
PROBLEMS CAUSES Ref

Measured value is low. Poor agitation A-25
（continued） Defect of Hemolyzer volume A-27
Fluid leakage of HGB block A-28
Mis-suction of sample A-32
Defect of Sample syringe volume A-33
55
Leakage of wiring tubes A-40

Loose tube joint A-41
Air leakage of Waste/Supply tank A-44
Poor reproducibility. Stains/bubbles of Cell A-8
Clogging of filter A-15
Poor agitation A-25
Mis-suction of sample A-32
Defect of Diluent syringe motion A-34
Defect of Hemolyzer syr. motion A-36
Infulence by outer noise A-46
Abnormality of WBC Proor quality of Hemolyzer A-3
Classification. Defect of Hemolyzer flow A-6
Poor agitation A-25
56
PROBLEMS CAUSES Ref

"CHECK CLOG" frequently Poor quality of Diluent A-1
appears. Poor quality of Cleaning solution A-2
Poor quality of Hemolyzer A-3
Stains/bubbles of Cell A-8
Defect of pressure board adjust A-18
Defect of Solenoid valve A-38
"CHECK DILUENT" frequently Defect of Water supply circuit A-4
appears. Defect of pressure board adjust A-18
Fluid leakage of Cell A-30
Defect of Pump/solenoid valve A-37
Fluid can not be discharged. Defect of Pump/solenoid valve A-37
Wrong piping A-42
All and every data are “0”. Mis-suction of sample A-32
Defect of Suction probe motion A-38
57

Wrong piping A-42
Reagent can not flow. Defect of Water supply circuit A-4
Defect of Cleaning solution flow A-5
Defect of Hemolyzer flow A-6
Fluid leaks and/or come out. Fluid leakage of Suction probe A-29
Fluid leakage of Cell A-30
Defect of Pump/solenoid valve A-37
Defect of Suction probe motion A-38
Wrong piping A-42
8-2-2 Causes and corrective action
CAUSES CORRECTIVE ACTION

A-1 Poor quality of Diluent. In case of bubbles in water supply tube,
supply water by EXTEND→WATER .
In case of NO Diluent, after changing it,
supply water by EXTEND→WATER .
In case that bubbles/floating materials are
found, after changing Diluent, supply water by
EXTEND→WATER .
A-2 Poor quality of In case that bubbles/floating materials are
Cleaning solution. found, after changing Solution, take cleanng
action by EXTEND→WASH .
A-3 Poor quality of In case that bubbles/floating materials are
Hemolyzer. found, after changing Hemolyzer, touch
EXTEND→HEMOLYZER EXCHANGE .
58
A-4 Defect of Water Supply water by EXTEND→WATER then,

supply circuit. confirm that supply water is in action.
Defect of Diluent syringe motion (Ref. A-34)
Defect of Pump/solenoid valve (Ref. A-37)
Bent/clog of wiring tube (Ref. A-39)
Leakage of wiring tubes (Ref. A-40)
Loose tube joint (Ref. A-41)
Wrong wiring (Ref. A-42)
Air leakage of Detection part (Ref. A-43)
Air leakage of Water/Supply tank (Ref A-44)
A-5 Defect of Cleaning Check cleaning solution bottle. In case of NO
solution flow. solution, replace with new solution.
Supply water by EXTEND→WATER then,
confirm that supply water is in action.
Wrong piping (Ref. A-42)
Air leakage of Detection part (Ref. A-43)
A-6 Defect of Hemolyzer Check Hemolyzer bottle. In case of NO
Flow. Hemolyzer, replace with new one.
touch SELECT MENU→MAINTENANCE→
LIQUID CIRCUIT TEST→SYRINGE ACTION.
and see that Hemolyzer flows into WBC Cell.
Defect of Hemolyzer syr. motion (Ref. A-36)
59
A-8 Stains/bubbles of Touch EXTEND→WASH for cleaning.

Cell. Remove Cell for disassembling cleaning
A-9 Clogging/stains/ Touch EXTEND→WASH for cleaning.
bubbles of Orifice. Touch SELECT MENU→MAINTENANCE→ LIQUID
CIRCUIT TEST → RBC CLOG REMOVE or WBC
CLOG REMOVE a few times.
Remove Orifice for cleaning.
Replace with new Orifice.
A-10 Defect of Orifice. Check and see if WBC Side(100u)and RBC
Side(80u) has been set upside down.
A-11 Stains/bubbles of Touch EXTEND→WASH for cleaning.
Electrode. Remove Electrode for cleaning.
A-12 Poor setting of See that Electrode not in contact with others.
Electrode. In case of cracks on Electrode, replace it with
new one.
A-13 Stains/bubbles of Touch SELECT MENU→SUB SELECTION→
probe cleaning. FUCTORY ADJUSTMENT→ ACTION TIME →
NOZZLEPROVE WADHIG TIME to change wash
time
Remove probe from the unit for
disasemblying cleaning.
A-14 Position of probe clean. While suction probe is in up position, adjust
that tip of probe is on the same hight with
inside-tapered area of probe cleaning part.
A-15 Clogging of filter. Remove Filter from the unit for claning.
Replace with new Filter.
A-16 Defect of suction volume. Measure Control blood, then touch
SELECT MENU→SUB SELECTION→ FACTORY
ADJUSTMENT→SUCTION VOLUME→WBC
SUCTION VOL or RBC SUCTION VOLUME to
readjust volume.
A-17 Mis-calibration. Take Calibraton by SELECT MENU→CALIBRATION
(Ref. User's guide 5-2 Calibraton)
A-18 Defect of pressure Adjust pressure sensor board.
board adjust. (Ref. 3-3-2 Pressure sensor board)
A-19 Defect of WBC Adjust WBC detecton board.
sensitivity adjust. (Ref. 3-3-11 WBC detection board)
A-20 Defect of RBC Adjust RBC detecton board.
sensitivity adjust. (Ref. 3-3-12 RBC detection board)
A-21 Defect of PLT Adjust RBC detecton board.
60
sensitivity adjust. (Ref. 3-3-12 RBC detection board)

A-22 Bubbles when touch EXTEND→WASH for cleaning.
diluent discharge. Adjust Pulse width of diluent syringe.
(Ref. 3-3-6 I/O board)

A-23 Bubbles in Water Touch EXTEND→WASH for cleaning.
supply circuit. Check Diluent bottle. In case of NO Diluent,
replace with new one.
Air leak of Detection part (Ref. A-43)
A-24 Bubbles in Touch EXTEND→HEMOLYZER EXCHANGE to
Hemolyzer circuit. remove bubbles in Hemoly.circuit to WBC cell.
Check Hemolyzer bottle. In case of NO
Hemolyzer, replace with new one.
A-25 Poor agitation. Defect of Pump/solenoid valve (Ref. A-37)
A-26 Defect of Hemolytic time. Touch SELECT MENU→SUB SELECTION→
FACTORY ADJUSTMENT→ACTION
TIME→MEASUREMENT START TIME to change
measure-start time.
A-27 Defect of Hemolyzer volume. Touch SELECT MENU→SUB SELECTION→
FUCTORY ADJUSTMENT→SYRINGE
VOLUME→HEMOLYZER SYRINGE to change
Hemolyzer syringe volume.
A-28 Fluid leakage of Hgb block. Remove HGB block from the unit for
disasemblying cleaning.
61
Wipe liquid around HGB block of WBC cell,

then refill it with silicone.
A-29 Fluid leakage of Suction Remove probe cleaning part from the unit
probe. for disasemblying cleaning.
Position of probe cleaning part (Ref. A-14)
Defect of connection cable (Ref. A-47)
A-30 Fluid leakage of Cell. Check and see if any cracks on Cell.
A-31 Defect of Sample. Check and see if it has become solid due to
insufficient aggitation right after sampling.
Stir well before measurement, re-measure.
Check if 12 hours+ have passed after sample
A-32 Mis-suction of sample. Be sure to put the tip of suction probe into
sample then take suction.
In case of less quantity of sample, sample
blood agian.

A-33 Defect of Sample syringe Measure Control blood, then touch
volume. SELECT MENU→SUB SELECTION→ FUCTORY
ADJUSTMENT→SYRINGE VOLUME→SAMPLE
SYRINGE to change volume.
Defect of sample syringe motion (Ref. A-35)
A-34 Defect of Diluent syringe Touch SELECT MENU→MAINTENANCE→ LIQUID
motion. CIRCUIT →SYRYNGE ACTION
to confirm Diluent syringe motion.
Adjust fitting of gears at Drive unit.
Adjust so that syringe part travels smoothly.
Check packings and change if worn and torn.
62
Check and see if sheild plate is surely

sensored. If not, adjust it.
Check sensor. If broken, replace with new one
A-35 Defect of Sample syringe Touch SELECT MENU→MAINTENANCE→
motion. LIQUID CIRCUIT→SYRINGE ACTION
to confirm Sample syringe motion.
A-36 Defect of Hemolyzer syringe Touch SELECT MENU→MAINTENANCE→
motion. LIQUID CIRCUIT→SYRINGE ACTION
to confirm Hemolyzer syringe motion.
63

A-37 Defect of Pump/ solenoid Remove Pump/solenoid valve for cleaning.
valve. Give +/- pressure to solenoid valve with
syringe tube to check leakage. If leaking,
replace with new solenoid valve.
A-38 Defect of Suction probe Check and see if suction probe is bent. If so,
motion. replace with new suction probe.
Check and see if sheild plate in probe travel
part is surely sensored. If not, adjust it.
Check pully timing belt, motor bearing. If worn
and torn, replace with new ones.
In case suction probe is stuck, connect
syringe tube with probe and give +/- pressure
Replace with new suction probe.
A-39 Bent/clogging of wiring Check bent/clogging of wiring tubes. If any
tubes. clogging found, clean and remove.
In case bent part is found, change new one.
A-40 Leakage of wiring tubes. In case holes/cracks are found on wiring tube,
replace with new ones.
In case joint part of tube and tube joint is
loose, replace with new tube.
A-41 Loose tube joint. Confirm that tube joint of Solenoid valve and
Tank etc. has been firmly tightened.
In case O-ring of joint part is worn and torn,
replace with new one.
A-42 Wrong piping. Compare the piping with the whole piping
diagram and correct.
Compare input/output of solenoid valve pump
with the whole piping diagram and correct.
64
A-43 Air leakage of Detection Check fixing bolts at Detection part. In case
part. they are loose, screw them again.
Check and see if any cracks at Detection part.
In case they are found, replace Detection part.
In case packing for Electrode is worn and
torn, replace with new one.
In case O-ring of joint part is worn and torn,
replace with new one。
A-44 Air leakage of Waste/Supply Check and see if any bad sealing of Water
tank. Supply/Waste tank. If found, fix it with silicone
and screw bottles tight.
A-45 Influence by inner noise. Correct loose fixing of sheild plate inside unit
Give washer to bolts and fix them tight.
A-46 Influence by outer noise. Put the unit away from noise generation area.
Use single receptacle and use Ground earth.
A-47 Defect of connection cable. Check and see if any damages on cables.
If any found, replace with new ones.
A-48 Wrong wiring. Compare the wiring with the whole wiring
diagram and correct.
Comfirm that silk and cable NO. of the board
is not wrong.
8-3 Troubles in operation
8-3-1 Problems and causes
SYMPTOMS CAUSES Ref

No power to instrument Power cord is unplugged B-1
Instrument fuse is blown B-2
Wrong wiring at power SW board B-3
Power switch is broken B-4
Lowering of power voltage B-5
Wrong wiring A-48
Uncontrolled CPU Lowering of power voltage B-5
CPU board is broken B-6
Lowering of 5V voltage B-7
65

Wrong wiring A-48
Improper key operation Switch is broken B-8
Touch panel LCD is brokn B-9
Wrong wiring A-48
Improper printer work Improper print paper B-10
Printer I/F board is broken B-11
Clogging in printer head B-12
Printer lever is in upper position B-13
Wrong wiring A-48
8-3-2 Causes and corrective action
CAUSES CORRECTIVE ACTIONS

B-1 Power cord is Turn the power to the unit is off, then plug in.
unplugged
B-2 Instrument fuse is Remove blown fuse and replace with new
blown fuse(5A).
66
B-3 Wrong wiring at Check wiring at Power switch board.

Power SW board
B-4 Power switch is Turn on and off the switch several times.
broken Change new power switch.
B-5 Lowering of power Confirm that power voltage is AC100V.
voltage
B-6 CPU board is Check and see if any damaged solder part on
broekn CPU board.
Change new CPU board.
B-7 Lowering of 5V Change new switching regulator.
voltage
B-8 Switch is broken Check and see if switch has been in pressed
position all the time.
Change new switch.
Change new switch board.
B-9 Touch panel LCD Change new Touch panel LCD.
is broken Change new LCD board.
B-10 Improper print Set print paper inside/outside correctly.
paper Change new print paper.
B-11 Printer I/F board Change new Printer I/F board.
is broken
B-12 Clogging in printer Remove clogged matter.
head Touch SELECT MENU→MAINTENANCE→
OUTPUT TEST→BUILT-IN PRINTER for test printing
to confirm printing motion.
B-13 Printer lever is in Lower head-up printer lever.
upper position Touch SELECT MENU→MAINTENANCE→
OUTPUT TEST→BUILT-IN PRINTER for
test printing to confirm printing motion.
67
9 REFERENCE
9-1 Mode Switch
Following is the explanation of Mode Switch located in the back of instrument.
No. FUNCTION ON OFF

1 Mode Maintenance Mode Normal Mode
2 Language English Japanese
3 - Not in use
4 EXT. Printer EPSON HP
5 - Not in use
6 - Not in use
7 - Not in use
8 - Not is use
1. Changing Mode
The following functions are added by Maintenance Mode.
 SUB SELECTION is displayed without any special operation.
 Automatic water supply is not operated when power is turned ON.
 Threshold and peak of WBC histogram are printed if SAMPLE No. is set in
advance.
 Exact value of background count of blank measurement is displayed.
After finishing maintenance, make sure to retrun the mode switch “OFF” position
and then turn the power OFF and ON again.
2. Language
Normally place the switch ON position.
3. Not in use
4. External Printer
Select the external printer you want to use. This mode is active with ROM
Ver. 2.36/5.02 or newer. If the ROM version is older than 2.36/5.02, only EPSON printer
Is usable.
 EPSON Control Code ESC/P Operation checked by LQ-300
LX-300+ (ROM Ver. 5.13 or newer)
 HP Control Code HP PCL Operation checked by hp deskjet 5650
Turn the power OFF and ON again after changing mode switch position.
68
5. Not in use
6. Not in use
7. Not in use
8. Not in use
9-2 Note for using external printer
The first printing right after connecting an external printer to PCE-210, some characters may be
missing or unnecessary characters may be printed on printing contents in the test printing or at
the end of measurement. To avoid the situation, please follow the undermentioned:
1. Make sure that the printer’s power is off when you connect a printer cable.
2. Make sure that you turn on PCE-210’s power first, then the printer’s power, at any time.
3. When you turn off the power, be sure that you turn off the printer, then PCE-210.
4. DO NOT turn on and off the power of PCE-210 while the printer’s power is ON.
5. Please also set a printer paper in the internal printer.
6. In case printer errors (running out of paper, opening the cover, etc.) happen, you can not
do the recovery operation by PCE-210, unlike the case you connect PCE-210 to PC.
69
EPSON HP
9-3 RS-232C Output
●Output format
Output Order Item Name Output digits Output code

1 STX STX
2 Sample No. 5 digits 00000
3 Date 10 digits yymmddhhmm
4 WBC 5 digits 000.0
5 LY 5 digits 000.0
6 MO 5 digits 000.0
7 GR 5 digits 000.0
8 LY% 5 digits 000.0
9 MO% 5 digits 000.0
10 GR% 5 digits 000.0
11 RBC 5 digits 00.00
12 Hgb 5 digits 000.0
13 HCT 5 digits 000.0
14 MCV 5 digits 000.0
15 MCH 5 digits 000.0
16 MCHC 5 digits 000.0
17 RDW 5 digits 000.0
18 PLT 4 digits 0000
19 PCT 5 digits 0.000
20 MPV 5 digits 000.0
21 PDW 5 digits 000.0
22 WBC Histogram 3 digits X 200 data 000 X 200
23 RBC Histogram 3 digits X 200 data 000 X 200
24 PLT Histogram 3 digits X 200 data 000 X 200
25 ETX ETX
　Forwarding Speed :　9,600 bps
70
　Start bit :　1 bit

　Data bit :　8 bit ASCII Code
　Parity bit 　 : None
　Stop bit　 : 1 bit
●Connecting cable
D-SUB9 Cable
External
PCE-210
Device
1 1
TxD 2 2 RxD
RxD 3 3 TxD
4 4
5 5 GND
6 6
GND 7 7
8 8
9 9
●Format Example
Data Data transfer

STX 02
No. 2 20 20 20 20 32
YY 00 30 30
MM 04 30 34
DD 01 30 31
hh 00 30 30
mm 15 31 35
WBC 14.2 20 31 34 2E 32
LY 11.1 20 31 31 2E 31
MO 1.3 20 20 31 2E 33
GR 1.8 20 20 31 2E 38
LY% 78.2 20 37 38 2E 32
MO% 9.2 20 20 39 2E 32
71
GR% 12.7 20 31 32 2E 37
RBC 4.43 20 34 2E 34 33
Hgb 15.1 20 31 35 2E 31
HCT 47.9 20 34 37 2E 39
MCV 108.1 31 30 38 2E 31
MCH 34.0 20 33 34 2E 30
MCHC 31.5 20 33 31 2E 35
RDW 15.5 20 31 35 2E 35
PLT 289 20 32 38 39
PCT 0.280 30 2E 32 38 30
MPV 9.6 20 20 39 2E 36
PDW 20.2 20 32 30 2E 32
WBC 0 20 20 30
Histogra 100 31 30 30 3 digits*200data
m 0 20 20 30
RBC 0 20 20 30
m 0 20 20 30
PLT 0 20 20 30
m 0 20 20 30
ETX 03
72
9-4 ROM Replacement
The following is how to replace ROM when the manufacturer asks to do so.
1. Before replacing the ROM, each parameter of present setting has to be stored. Please
save the necessary stored sample data because all of them will be deleted by changing
ROM.
2. Press “PRINT” switch on the SUB SELECTION so that the parameters will be printed out
from the built-in printer.
3. Set the DIP switch # 1 of the back side of equipment “ON”, then turn off the power and
pull out the power supply code. Replace the ROM.
4. Turn the power on. Measurement stand-by screen will be displayed without a water
supply cycle.
5. Go to SUB SELECTION – BASICAL SETTING – INITIALIZATION to initialize the

equipment. At this time, SUB SELECT MENU can be displayed without any special
operation.
6. Now each parameter returned to the initial value, so please input the value again. After
finishing re-input, turn DIP switch # 1 “OFF” and turn off the power.
9-5 Clog Detection
Clog Detection Method 1
The following calculation and detection is carried out in software when RBC value is more
than 500,000 and Hgb value is more than 3.0 mg/dl.
1. Total RBC value is obtained.

2. 70% value of total RBC is calculated
3. The value obtained in above No. 2 is divided by RBC sample aspiration volume.
4. When total measured value for 1 second is smaller than the value obtained in above No.
3, PCE-210 detects it as RBC clog.
The following calculation and detection is carried out in software when WBC value is more
73
than 1,000 and Hgb value is 3.0 mg/dl.
1. Total WBC value is obtained.

2. 70% value of total WBC is calculated.
3. The value obtained in above No. 2 is divided by WBC sample aspiration volume.
4. When total measured value for 1 second is smaller than the value obtained in above No.
3, PCE-210 detects it as WBC clog.
Clog Detection Method 2
Number of the blood cell pulse is counted in each cycle fixed during measurement and PCE-
210 makes comparison in the number of pulses for each two adjoining cycles. If there is more
than 30% decrease detected in number of pulses, for example, if the previous cycle has shown
100 pulses detected and the following cycle shows 70 pulses only, PCE-210 concludes that
there is abnormality in the detection (measurement) part, showing messages such as “RBC
CLOG”, “WBC CLOG” and etc. However, those error messages are displayed only after the
measurement cycle is completed. Even if the abnormality is detected in the middle of the
measurement, counting itself is continued until the end of the measurement cycle. Users are
also able to see “*” in the left side of parameter names both in the display and in print-outs
when there was abnormality, such as clogging.
100%
70%
9-6 Measurement Control for Pressure Volumetric Method
Fixed volume measurement by pressure control

A negative pressure pump of PCE-210 makes aspiration pressure to the fixed value of –
150mmHg just before aspirating diluted sample. This fixed pressure is kept and controlled all
the time through measurement operation by pressure sensor. Under the circumstance of the –
74
150mmHg pressure, a valve for sample aspiration is opened for the measurement. The
aspiration time is set in advance as a factory adjustment and never varies in each individual
measurement. Therefore, volume of the diluted sample can be fixed same for each
measurement due to the aspiration made under condition of fixed pressure for the fixed time.
In case there is variation seen in pressure during measurement, PCE-210 detects its
abnormality in aspiration flow and stops measurement.
75

Service Manual For Erma Pce210

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PCE-210SME Ver. 5.

FULL AUTOMATIC BLOOD CELL COUNTER

Installation Instruction &

For safe use of PCE-210, please read this Installation Instruction

FULL AUTOMATIC BLOOD CELL COUNTER

2 COMPONENTS & FUNCTION OF THE UNIT 3

3-1 OPENING CARTON BOXES 6

5-1 THE WHOLE PIPING DIAGRAM 30

6-1 FACTORY ADJUSTMENT 35

7-1 MAINTENANCE FOR LONG TIME STORAGE 42

8-1 ERROR MESSAGE 45

9-1 MODE SWITCH 57

1 SAFETY NOTES TO USERS

Symbols and their meaning are as follows.

If treated mistakenly, a danger to cause death or wound is

Power Unit outlet should be independent.

Not to plug or unplug with wet hands.

Not to damage power cords and connecting cables, etc.

Not to use damaged power cords and connecting cables, etc.

Stop operation when in extraordinary motion.

pointed pen may cause breakdown or damage.

2 COMPONENTS & FUNCTION OF THE UNIT

4.Reagent holder panel

10.LCD Angle Adjustment

13.Connecter of external printer

2-3 Right side view

18.Water supply joint

19.Cleaning solution joint

20.Connector for waste solution tube &

21.Detector maintenance panel

22.Liquid circuit maintenance panel

2-4 Hemolysys reagent holding space

23. Heamolysis reagent nozzle

2-5 Maintenance space of liquid circuit

27.Pinch valve (XB4)

28.Pinch valve (XB5)

3-1 Opening carton boxes

・Take PCE-210 unit and its accessories neatly out of cartons.

PCE-210 unit contains the following accessories.

No. Descriptions Q’ty

1. Use the unit in the environment where room

2. Install the unit on a flat and sturdy table that is not

3. For installation, avoid the place that is subject to

4. Do not put a container containing reagent, water,

5. Install the unit in a place where no corrosive gas

6. Do not use the unit in a dusty place, as it is a

7. Electricity consumption is approx. 180Ｗ, voltage is +/-10% against the

3-4-1 Connecting power code

Put a connector of the attached power

3-Pin type outlet 2-Pin type outlet

・Be careful not to let ground tube bend over.

Waste solution bottle Cleaning solution bottle

Dil uent bottle

2. Connection of diluent bottle

3. Connection of Cleaning solution bottle

4. Connection of waste solution bottle

5. Connection of float sensor

6. Connection of Hemolyzer bottle

3-4-3 Setting print paper

Paper holding lever

Roll paper holder Paper insertion

Paper holding lever

Turn power to the unit on.

3-1　OPENING CARTON BOXES 6

5-1　THE WHOLE PIPING DIAGRAM 30

7-1　MAINTENANCE FOR LONG TIME STORAGE 42

2-3　Right side view

　18.Water supply joint

　19.Cleaning solution joint

2-4　Hemolysys reagent holding space

2-5　Maintenance space of liquid circuit

3-1　Opening carton boxes

3-4-1　Connecting power code

　 Waste solution bottle 　 Cleaning solution bottle

3-4-3　Setting print paper

Roll paper holder　　　　　　　　　　　　　　　　　　　　　　Paper insertion

　　　　　　　　　　　　　　　　　　　　　　　　　　　　Paper holding lever

3-6-3　Filling of cleaning solution

4-2　The Whole wiring Diagram

4-3　List of electric circuit boards

4-4-3　Printer I/F board

4-4-4　Panel switch board

4-4-9　Power switch board

4-4-11　WBC detection board

4-4-12　RBC detection board

5-1　The Whole piping Diagram

5-2　List of electric parts for liquid circuit