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Asexual Reproduction
● The period through which a certain organism lives is known
as its life span.
● Reproduction is the process by which every organism
ensures its continuity.
● It is the process through which organisms produce young
ones, which in turn mature to give rise to their young ones.
● Reproduction can be:
○ Asexual − Only one individual is involved
○ Sexual − Two individuals (male and female) are involved
Asexual Reproduction
● Binary Fission − In this process, the cell divides into halves, and
each half develops into an adult (example: Amoeba, Paramecium).
● Budding − In this process, the cell divides unequally to form buds,
which remain attached to the parent initially, and then detach and
develop into a mature cell (example: yeast).
Pre-Fertilisation Events
Gametogenesis
.Gamete Transfer
Zygote
Embryogenesis
Androecium
● Structure of microsporangium
○ The microsporangium is surrounded by four wall layers
(epidermis, endothecium, middle layers, and tapetum).
○ The outer three layers are protective and help in
dehiscence of anther to release the pollen grains. The
tapetum provides nourishment to the developing pollen
grains.
○ In the young anther, the sporogenous tissue forms the
centre of each microsporangium.
Microsporogenesis
Pollen grains
Female Gametophyte
Pollination
● It is the process of transfer of pollen grains from the anther
to the stigma.
● Depending on the source of pollen, pollination can be divided
as follows:
○ Autogamy − It is the transfer of pollen grains from the anther
to the stigma of the same flower. Autogamy requires the anther
and the stigma to lie close. It also requires synchrony in the
pollen release and stigma receptivity.
Plants like Viola, Oxalis, etc., produce two kinds of
flowers—chasmogamous flowers (with exposed
anther and stigma) and cleistogamous flowers
(which do not open at all and only autogamy occurs).
○ Geitonogamy − It is the transfer of pollens from the anther of
one flower to the stigma of another flower in the same plant.
Genetically, it is similar to autogamy, but it requires pollinating
agents.
○ Xenogamy − It is the transfer of pollen grains from the anther
to the stigma of a different plant. Pollination causes genetically
different types of pollens to be brought to a plant.
Agents of Pollination
Artificial Hybridisation
Double Fertilisation
● When the pollen grains fall on the stigma, the pollen tube
enters one of the synergids and releases two male gametes.
● One of the male gametes moves towards the egg cell and
fuses with it to complete the syngamy to form the zygote.
● The other male gamete fuses with the two polar nuclei and
forms triploid primary endosperm nucleus (PEN). This is
termed as triple fusion.
● Since two kinds of fusion—syngamy and triple fusion—take
place, the process is known as double fertilisation, and is
characteristic of flowering plants.
● After triple fusion, the central cell becomes the primary
endosperm cell (PEC).
● The primary endosperm nucleus gives rise to the
endosperm, while the zygote develops into the embryo.
Post-Fertilisation Events
It includes development of endosperm and embryo, and
maturation of ovules into seeds and ovaries into fruits.
Formation of Endosperm
Development of Embryo
Development of Fruits
Ovaries
● They are the primary female sex organs. They produce the
ovum and other ovarian hormones.
● They are located in the lower abdomen, and are 2 to 4 cm in
length.
● They are connected by ligaments to the pelvic walls and to
the uterus.
● Each ovary is covered by epithelium, and contains the
ovarian stroma.
● The ovarian stroma is made up of:
○ Peripheral cortex
○ Inner medulla
Oviducts
Uterus
External Genitalia
● Consists of:
○ Mons pubis − Fatty tissue covered by skin and pubic hair
○ Labia majora − Extends from mons pubis and surrounds the
vaginal opening
○ Labia minora − Fold of skin beneath the labia majora
○ Hymen − Partially covers the vaginal opening
○ Clitoris − Lies at the junction of labia minora
Mammary Glands
Spermatogenesis
Structure of a Sperm
Oogenesis
● The LH and FSH are at their peak in the middle of the cycle
(14th day), and cause the rupture of the Graffian follicles to
release ovum. This phase is called the ovulatory phase.
● The remains of the Graffian follicles get converted into the
corpus luteum, which secretes progesterone for the
maintenance of the endometrium.
● In the absence of fertilisation, the corpus luteum
degenerates, thereby causing the disintegration of the
endometrium and the start of a new cycle.
● In humans, the menstrual cycle ceases to operate at the age
of 50 years. This phase is known as the menopause.
Pregnancy
Population Explosion
Birth Control
● It is essential to ensure birth control to check population explosion.
● Successive governments have come out with various programmes that
encourage smaller families by means of various contraceptive methods.
● Contraceptive methods are of two types:
○ Natural means of contraception
○ Artificial means of contraception
Natural Methods
Artificial Methods
Infertility
● Many couples all over the world are unable to produce children.
● Some of the reasons for infertility are:
○ Congenital diseases
○ Drugs
○ Immunological and Psychological factors
● Specialised fertility clinics can help diagnose and treat infertility.
● The couples can be assisted to have children through techniques called
assisted reproductive technologies (ART).
● In vitro fertilisation (IVF): Fertilisation takes place outside the body (test
tube baby). The following techniques are included in IVF.
○ ZIFT − It stands for zygote intra fallopian transfer. In ZIFT, the sperm from a
donor male and the ova from a donor female are fused in the laboratory. The
zygote so formed is transferred into the fallopian tube at the 8 blastomeres stage.
○ IUT − It stands for intra uterine transfer. In this technique, embryos with more
○ GIFT − It stands for gamete intra fallopian transfer. In GIFT, females who cannot
produce ovum, but can provide suitable conditions for the fertilisation of ovum,
○ ICSI − It stands for intra cytoplasmic sperm injection. In this method, sperm is
husband or a donor is injected into the vagina or uterus. This cures the infertility
arising from the inability of the male partner to ejaculate, or due to low sperm
count.
PRINCIPLES OF INHERITANCE AND VARIATION
Genetics
Inheritance
Variation
Mendel’s Experiments
Two alleles code for a pair of two contrasting traits. (e.g., tall and dwarf)
Monohybrid Cross
● Cross that considers only a single character (e.g., height of the part)
●
● Studying the cross:
○ TT, tt, and Tt are genotypes while the traits, tall and dwarf, are
phenotypes.
○ T stands for tall trait while t stands for dwarf trait.
○ Even if a single ‘T’ is present in the genotype, phenotype is ‘tall’.
When ‘T’ and ‘t’ are present together, ‘T’ dominates and
suppresses the expression of ‘t’. Therefore, T (for tallness) is
dominant trait while t (for dwarfness) is recessive trait.
○ TT and tt are homozygous while Tt is heterozygous.
○ From the cross, it can be found that alleles of parental pair
separate or segregate from each other and only one allele is
transmitted to the gamete.
○ Gametes of TT will have only T alleles; gametes of tt will have
only t alleles, but gametes of Tt will have both T and t alleles.
● Punnett square
○ Graphical representation to calculate the probability of all
possible genotypes of offsprings in a genetic cross
○ Possible gametes are written on two sides, usually at top row
and left columns, and combinations are represented in boxes.
○ With the help of Punnet square, genotypic ratio in F 2 generation
can be found. From the above given Punnet square, it is evident
that genotypic ratio TT: Tt: tt is 1:2:1.
Law of Dominance
Test Cross
● This law states that the two alleles of a pair segregate or separate
during gamete formation such that a gamete receives only one of the
two factors.
● In homozygous parents, all gametes produced are similar; while in
heterozygous parents, two kinds of gametes are produced in equal
proportions.
Incomplete Dominance
What is Dominance?
I A
I A
I A
I A
A
I A I B I A I B AB
I A i I A i A
I B I A I A I B AB
I B I B I B I B B
I B
i I B
i B
i i ii O
Mutation
Pedigree Analysis
Genetic Disorders
Mendelian Disorders
○ Phenylalanine Tyrosine
The enzyme responsible for this conversion gets mutated.
○ Phenylalanine accumulates. Then,
Phenylalanine → Phenylpyruvic acid → Accumulates in brain →
Mental retardation
○ Phenylpyruvic acid also gets excreted through urine since
kidneys poorly reabsorb it.
Chromosomal Disorders
Chromosomal Disorders
● Total number of chromosomes in humans = 46 (23 pairs)
● Total 23 pairs = Autosomes (22 pairs) + Sex chromosomes (1 pair)
● Monosomy − Lack of any one pair of chromosomes
● Trisomy − Inclusion of an additional copy of chromosome
● Aneuploidy − Loss or gain of chromosomes due to the failure of segregation of
chromatids during cell division
● Down’s Syndrome
○ Cause: Presence of an additional copy of chromosome 21
(Trisomy of 21)
○ Affected individual has short stature, small, round head,
furrowed tongue, partially opened mouth, palm crease,
congenital heart disease and mental retardation.
● Klinefelter Syndrome
○ Cause: Additional copy of X chromosome, i.e., 47 chromosomes
(XXY)
○ Affected individual has an overall masculine development with
gynaecomastia; individual is sterile
● Turner’s Syndrome
○ Cause: Absence of one X chromosome, i.e., 45 chromosomes
(XO).
○ Affected females are sterile; have rudimentary ovaries;
secondary sexual characters are absent
MOLECULAR BASIS OF INHERITANCE
DNA : Structure of Polynucleotide Chain
● DNA − Polymer of deoxyribonucleotides
● Nucleoside = Nitrogenous base + Pentose sugar (linked through N −
glycosidic bond)
Example − adenosine, deoxyadenosine, cytidine, etc.
● Nucleotide = Nucleoside + Phosphate group (linked through
phosphodiester bond)
● Many nucleotides link together through 3′ − 5′ phosphodiester bond
to form polynucleotide chain (as in DNA and RNA).
● In course of formation of polynucleotide chain, a phosphate moiety
remains free at 5′ end of ribose sugar (5′ end of polymer chain) and
one -OH group remains free at 3′ end of ribose (3′ end of polymer
chain).
● Scientists involved
○ Friedrich Meischer − First identified DNA as an acidic substance
present in nucleus and named it as ‘Nuclein’
○ Wilkins and Franklin − Produced X-ray diffraction data for DNA
structure
○ Watson and Crick − Proposed double helix structure model for DNA
based on X-ray diffraction data
○ Erwin Chargaff − Proposed that in ds DNA, ratios A:T and C:G remain
same and are equal to one
= ~ 2.2 m
Transforming Principle
● Griffith’s experiment
● Live R strain in the presence of heat-killed S strain produce virulence
because somehow R strain bacteria is transformed by heat-killed S
strain bacteria. Hence, it was concluded that there must be transfer of
genetic material.
●
● This suggests that DNA has to be the genetic material.
DNA RNA
Transcriptional Unit
Gene
● The DNA sequence which codes for tRNA or rRNA molecule defines a
gene.
● Cistron − Segment of DNA that contains the genetic code for a single polypeptide
● The structural genes could be of two types:
○ Monocistronic (mostly in eukaryotes)
○ Polycistronic (mostly in prokaryotes)
● Monocistronic genes have two parts:
○ Exon − Sequences that code for a particular character and is expressed in
a matured and processed mRNA
○ Intron − Interrupting sequences that do not appear in a mature and
processed mRNA
● Regulatory genes − Sequences that do not code for anything, but have
regulatory functions
Types of RNA
Transcription Process
● In prokaryotes:
○ There is no clear demarcation between cytosol and nucleus.
Therefore, translation can begin even before transcription is
completed. Thus, in prokaryotes, transcription and translation
are coupled.
● In eukaryotes:
○ Three different kinds of RNA polymerases are present.
RNA polymerase I transcribes rRNA.
RNA polymerase II transcribes hnRNA (mRNA precursor).
RNA polymerase III transcribes tRNA, snRNA, and srRNA.
○ The precursor of mRNA, i.e. hnRNA, contains both introns and
exons. Introns are removed and exons are joined by a process
called splicing.
○ Capping − In this, methyl guanosine triphosphate is added to the 5′ end of
hnRNA.
○ Tailing − In this, adenylate residues are added to the 3′ end of hnRNA.
○ When hnRNA is fully processed, it is known as mRNA, which is
transported out of the nucleus to get translated.
Genetic Code and Study of Mutations
Genetic Code
● Genetic code directs the sequence of amino acids during the synthesis
of proteins.
● George Gamow proposed that if 20 amino acids are to be coded by 4
bases, then the code should be made up of three nucleotides. 4 3 = 64
(42 = 16), which is less than 20; so, the codon was proposed to be
triplet.
● Har Gobind Khorana developed a chemical method to synthesise RNA
molecules with defined combination of bases.
● Nirenberg developed cell-free systems for protein synthesis, which
helped the code to be deciphered.
● The enzyme known as Severo Ochoa enzyme (polynucleotide
phosphorylase) helped to polymerise RNA with defined sequences in a
template independent manner.
● It finally gave rise to the checker-board for genetic code.
● Salient features of genetic code:
○ Codon is triplet. 43 = 64 (61 codons code for amino acids while 3
are stop codons)
○ One codon codes for a single specific amino acid. Codons are
unambiguous.
○ Codons are degenerate since some amino acids are coded by
more than one codon.
○ Genetic code is universal. 1 codon codes for same amino acid in
all species.
○ Codons are read continuous. They lack punctuations.
○ AUG has dual functions − Codes for Methionine and acts as a start codon
tRNA
Translation
Lac Operon
● All genes involved in lac operon are required for metabolism of lactose.
● Inducer − Lactose acts as an inducer for lac operon since it regulates the
switching on and off of the operon.
● If lactose is provided to the growth media of bacteria in absence of any
other carbon source, then it is transported inside the cells by
permease.
● For permease to be present and lactose to enter inside the cells, low
level of expression of lac operon must be present all the time.
Genome Sequencing
● DNA from the cells is isolated and is randomly broken into fragments
of smaller sizes.
● These fragments are cloned into suitable host using vectors.
● Cloned fragments amplify in the host. Amplification facilitates an easy
sequencing.
● Common vectors used − BAC (Bacterial artificial chromosomes) and YAC (Yeast
artificial chromosomes)
● Common hosts − Bacteria and yeasts
● Automated sequencers are used to sequence these smaller fragments
(Sanger sequencing).
● The sequences so obtained are arranged based on overlapping regions
within them (alignment).
● Alignment of the sequences is also done automatically by computer
programs.
● Then these sequences are annotated and assigned to each
chromosome.
DNA Fingerprinting
Introduction
Theories of Evolution
Beagle and concluded that all existing living forms share similarities among
themselves and also with other life forms, which existed millions of years
ago of which many are extinct.
● The evolution of life forms has been gradual and those life forms
better fit in environments that leave more progeny. This is called
natural selection and is a mechanism of evolution.
● Alfred Wallace working in the Malay Archepelago also came to the
same conclusion.
Evidences of Evolution
● Fossils − They represent plants and animals that lived millions of years ago and
are now extinct. Different aged rock sediments contain fossils of different life-
forms, which probably died during the formation of the particular sediment.
similarities and differences between living forms of today and that of the
are:
Though they perform different functions, they are anatomically similar. This is
performs the same function (e.g., the wings of butterflies and birds). This is called
convergent evolution.
● Adaptive melanism − In England, it was noted that before industrial
revolution, the number of white-winged moths was more than that of dark
melanised moths. The explanation was that after industrialization, the tree trunks
became darker with deposits of soot and smoke and hence, the number of dark
moths increased in order to protect themselves from predators while the white-
● Similarly, the herbicide and pesticide resistant plants and animals and
antibiotic resistant bacteria are some of the evidences that point
towards evolution.
Adaptive Radiation
● During his exploration of the Galapagos Islands, Darwin noticed that
there were many varieties of finches in the same island.
● They varied from normal seed eating varieties to those that ate
insects.
● This process of evolution starting from a single point and radiating in
different directions is called adaptive radiation.
● The other example for this is the evolution of the Australian marsupials
from a single ancestor. Placental mammals also exhibit similarities to
their corresponding marsupial. Example: placental wolf and the
Tasmanian wolf
● When more than one adaptive radiation occurs in an isolated
geographical area, the phenomenon is called convergent evolution.
Mechanism of Evolution
● Darwin did not quite explain how evolution gave rise to different
species of the same organism.
● Mendel mentioned about inheritable factors, which influenced the
phenotype of an organism.
● Hugo de Vries based on his work on evening primrose suggested that
variations occurred due to mutations.
● Mutations are random and directionless while the variations that
Darwin talked about were small and directional. Hugo de Vries gave
the name saltation (single step large mutation) to the mutations
which brought about speciation.
Hardy-Weinberg Principle
● The frequency of occurrence of alleles of a gene in a population
remains constant through generations unless disturbances such as
mutations, non-random mating, etc. are introduced.
● Genetic equilibrium (gene pool remains constant) is a state which
provides a baseline to measure genetic change.
● Sum total of all allelic frequencies is 1.
● Individual frequencies are represented as p and q such as in a diploid,
where p and q represent the frequency of allele A and a.
● Cellular life forms occurred on earth about 2000 million years ago.
● Some of these cells had the ability to produce oxygen through
reactions similar to photosynthesis.
● Slowly, single-celled organisms became multicellular.
● Seaweeds and some plants probably existed around 320 million years
ago.
Evolution of Animals
● Animals evolved about 500 million years ago. The first of them to
evolve were invertebrates.
● Jawless fishes evolved around 350 million years ago.
● Some of the fishes could go on land, and then come back to water.
These were the first amphibians. In 1938, a fish Coelacanth, which
was thought to be extinct, was caught in South Africa. This variety of
fish, called lobefins, is believed to have evolved into the first
amphibians.
● Amphibians evolved into reptiles. In the next 200 million years,
reptiles of different sizes dominated the earth. However, about 65
million years ago, some of them such as dinosaurs disappeared.
● The first among the mammals were small shrew-like mammals.
● During continental drift when North America joined South America,
primitive mammals suffered, but pouched mammals of Australia
survived the same drift because of lack of competition from other
mammals.
Origin and Evolution of Man
years ago
● Health is the state of complete physical, mental, and social well being.
● Health increases productivity and ensures longevity.
● Balanced diet
● Personal hygiene
● Exercise
● Awareness about prevention and control of diseases
● Proper waste disposal and control of vectors
● Vaccination
Pathogenic Diseases
● Pathogens are the parasites that enter the human body through
various means, then multiply, and interfere with normal vital activities.
Bacterial Diseases
● Typhoid
○ Pathogen − Salmonella typhi
○ Spreads through − Contaminated food and water
○ Site of infection − Small intestine
○ Symptoms − High fever, stomach pain, headache, loss of appetite,
constipation, and intestinal perforations in severe cases
○ Confirmatory test − Widal test
● Pneumonia
○ Pathogens − Streptococcus pneumoniae and Haemophilus
influenzae
○ Spreads through − Droplets/aerosols released from infected person,
sharing of glasses or utensils
○ Site of infection − Alveoli (gets filled with fluid, difficulty in breathing)
○ Symptoms − Fever, chills, cough, headache, lips and nails become grey in
severe cases
Viral Diseases
● Common cold
○ Pathogen − Rhino viruses
○ Site of infection − Nose and respiratory passage
○ Spreads through − Droplets released from coughing or sneezing, or
contaminated objects
○ Symptoms − Nasal congestion and discharge, sore throat, cough,
headache, tiredness
Protozoan Diseases
● Malaria
○ Pathogen − Plasmodium sps. (P.vivax, P. falciparum, P. malaria)
○ Vector − Female Anopheles mosquito
○ Symptoms − High grade fever, chills
● Amoebiasis
○ Pathogen − Entamoeba histolytica
○ Vector − Housefly
○ Site of infection − Large intestine
○ Symptoms − Constipation, abdominal pain, cramps, stools with mucous,
and blood clots
Fungal Diseases
● Ringworms
○ Pathogens − Genera Microsporum, Trichophyton, and
Epidermophyton
○ Spreads through − Towels, clothes, comb (Fungus is acquired from soil)
○ Symptoms − Appearance of dry, scaly lesions on various body parts with
intense itching
● Elephantiasis (filariasis)
○ Pathogen − Wuchereria (W.malayi and W.bancrofti)
○ Spreads through − Bite of female mosquito vector
○ Symptom − Chronic inflammation of the organs, usually the lymphatic
vessels of lower limb
Types of immunity
● Active Immunity
○ This is the naturally acquired immunity produced in the host
body in response to an antigen.
○ Immunization and body naturally getting immune to a microbe
that had caused infection previously are examples of active
immunity.
● Passive immunity
○ When ready-made antibodies are provided to an individual to
protect against foreign agents
○ Colostrums present in mother’s milk contain IgA. Also, the foetus
gets antibodies from mother through placenta.
● Allergies
○ Exaggerated immune response to certain antigens present in
environment
○ Allergens − Substances in response to which allergy is produced
E.g., dust, pollen, etc.
○ Antibodies involved − IgE type
○ During allergic reactions, chemicals such as histamines and
serotonins are released.
○ Symptoms − Sneezing, watery eyes, difficulty in breathing, etc.
○ Allergy test − Patient is injected with small doses of allergens to monitor
his response.
○ Antihistamines, adrenalins, and steroids may be given so that
the symptoms of allergy subside.
● Autoimmunity
○ In autoimmunity, body generates immune response against its
own cells.
○ Reasons − Genetic and other unknown reasons
○ Example − Rheumatoid arthritis is an autoimmune disease.
Cancer
Cannabinoids
Cocaine
Nicotine
● Alcohol / drug abuse normally starts in adolescence (period between 12-18 yrs −
transition phase between childhood and adulthood).
● Many adolescents are motivated towards drugs/ alcohol due to
curiosity and experimentation.
● Peer pressure, academic stress, unstable family structure further
incline youth towards alcohol/ drug abuse.
● Perception of consuming alcohol / drug being cool and progressive and
use of alcohol/drug in television, movies, etc. further promote this
habit.
Animal Husbandry
Introduction
Farm Management
Animal Breeding
● Breed − A group of animals related by descent and similar in most characters such as
general appearance, features, size, etc.
● Aims of breeding:
○ To increase yield of animals
○ To improve desirable qualities in produce
● Breeding is of two types − inbreeding and out-breeding.
● Inbreeding
○ Mating of more closely related individuals of same breed for four
generations
○ Superior females and superior males are identified and mated.
○ Superior females − Produce more milk per lactation
○ Superior males − Give rise to a superior progeny
○ Inbreeding increases homozygosity. It evolves a pure line.
○ It accumulates superior genes, but also threatens to accumulate
harmful recessive genes
●
○ Continuous inbreeding may reduce fertility and productivity. This
problem is called inbreeding depression.
○ Out-breeding provides a solution to inbreeding depression.
● Out-breeding − It includes out-crossing, cross-breeding, and interspecific hybridisation.
○ Out-crossing − It is the mating between animals of same breed, but not
having common ancestors for 4 − 5 generations. It is usually used for animals,
which have below average productivity and growth rate.
○ Cross-breeding − It is the mating between superior male of one breed with
superior female of another breed. Superior qualities of both the breeds combine
and this is known as hybrid vigour. The progeny so formed is called hybrid. A
hybrid may be used as it is or may be further subjected to inbreeding.
Example: Hisardale sheep is a hybrid of Bikaneri ewes and Marino rams.
○ Interspecific Hybridization − Males and females of different, but related
species are mated. Progeny has desirable features of both the species.
Example − Mule is an interspecific hybrid of donkey and horse.
● Artificial Insemination
Semen is collected from the male and injected into the reproductive tract of
the female.
Apiculture
Fisheries
Plant Breeding
What is Plant Breeding?
Sugarcane
Millets
● Hybrid maize, jowar and bajra have been successfully developed in India.
● These varieties are high yielding and resistant to water stress.
Pest-Resistant Crops
● A large number of people all over the world suffer from micronutrient, protein
and vitamin deficiencies (hidden hunger) since they cannot afford to buy food
rich in these nutrients.
● Such deficiencies lead to diseases, mental retardation and reduced lifespan.
● An alternative to this problem is to breed crops that are rich in nutrients.
● This approach is called bio-fortification of crops. Objectives of bio-fortification are to
improve −
○ Protein content and quality
○ Oil content and quality
○ Vitamin content
○ Micronutrient and mineral content
● Examples −
○ Maize hybrids developed in the year 2000 have twice the amount of
lysine and tryptophan compared to other maize hybrids.
○ Atlas 66 (a wheat variety having higher protein content)
Tissue Culture
E.g., protoplasts of potato and tomato have been actually fused to form a
Pomato, but this plant is not commercially viable.
○ Milk Curd
○ LAB produces acids that coagulate and partially digest milk proteins.
○ Small amount of curd that is added to the milk for curdling acts as an
inoculum containing thousands of LABS, which further multiply.
○ LAB enhances the nutritional value of milk by increasing Vitamin B 12.
○ LAB present in stomach prevents infections.
● Fermentation
○ Dosaand idli dough is fermented by bacteria, which produces CO2gas
and gives it a puffed-up appearance.
○ Dough used for making breads is fermented by baker’s yeast
(Saccharomyces cerevisiae).
● ‘Toddy’ (a traditional drink from South India made by fermenting sap from
palm trees)
● Cheese making
○ The bacterium Propionibacterium sharmanii is used in ‘Swiss cheese’ to
give it its characteristic holes by producing large amount of carbon
dioxide.
○ ‘Roquefort cheese’ is ripened by growing certain fungi on them to give
them their specific flavour.
Industrial applications
sedimentation
decreases
● What is a floc?
Floc = Bacteria + Fungal filaments (in a mesh-like structure)
○ When BOD and hence pollution is reduced, effluent is passed into a
settling tank. Here, flocs settle down and it is known as Activated
Sludge.
○ In anaerobic sludge digesters, anaerobic bacteria act on the activated
sludge to produce biogas (CH4, CO2, H2S).
○ The effluent from secondary treatment plant is released into water
bodies.
○ Microbial technology for sewage treatment is so effective that no
human technology has been able to beat it till date.
● Biogas plant is usually set up in rural areas since cow dung is available in
abundance there.
● Biogas is used for cooking and lighting.
● Biogas technology in India is due to the efforts of:
○ IARI (Indian Agricultural Research Institute)
○ KVCI (Khadi and Village Industries Commission)
Example − Ladybirds and dragonflies are used to get rid of aphids and mosquitoes.
● Microbes can also act in the same manner. Bacillus thuringiensis (Bt) is used
to control butterfly caterpillars.
● This bacterium is available in sachets as dried spores, which are sprayed on
the crops. The spores get into the gut of the larvae and kill it while the other
insects remain unperturbed.
● By methods of genetic engineering, the genes of B. thuringiensis responsible
for killing the larvae have been incorporated into the plants.
● Cotton plant with Bt gene incorporated is called Bt-cotton.
● The fungus Trichoderma living in roots of plants acts as a bio control agent
against several plant pathogens.
● Baculoviruses, particularly genus Nucleopolyhedrovirus,are also used as
narrow spectrum insecticidal agents.
● Bio control agents are particularly useful when useful insects are required to
be conserved under IPM (integrated pest management programmes).
Microbes as bio-fertilizers
Cyanobacteria such as Nostoc, Anabaena, etc. also fix atmospheric nitrogen and act
as bio-fertilizers especially in paddy fields.
Biotechnology: Principles and processes
What is biotechnology?
Genetic Engineering
Gel Electrophoresis
Competent host
● Competent host refers to the bacterial cells that have the ability to
take up the vector (containing Recombinant DNA).
● Methods to introduce recombinant DNA into competent host:
○ Cells are treated with divalent cations (e.g. Ca 2+). Then, these
cells are incubated with recombinant DNA on ice, followed by
heat shock (at 42º), and then putting them back on ice. By this,
bacteria are able to take up recombinant DNA.
○ Microinjection − Recombinant DNA is directly injected into the nucleus
of animal cell.
○ Biolistics (Gene Gun) − Cells are bombarded with high velocity micro
particles of gold or tungsten.
○ Disarmed vector as in case of A. tumefaciens and retrovirus
Processes of RDT
Isolation of Genetic Material (DNA)
● For the processes of RDT, DNA must be available in its pure form.
● First of all, cells are treated with specific chemicals to break open the
cell to release cellular components such as DNA, RNA, proteins, etc.
This is done by enzymes such as lysozymes (bacterial cell), cellulase
(plant cell), and chitinase (fungal cell).
● Contaminants such as RNA and proteins are digested with the help of
ribonucleases and proteases respectively.
● Addition of chilled ethanol ultimately precipitates out the purified DNA,
which can be seen as collection of fine threads in the suspension.
● This is the stage for which the recombinant DNA was produced.
● The cell containing recombinant DNA will produce a novel protein
product (desirable product/Recombinant protein).
● For large scale production of the desirable product (antibiotics,
vaccines, enzymes), optimum conditions are to be provided.
● Continuous culture − Used culture media is drained from one side and fresh
culture media is added from the other side.
○ Cells are kept throughout in their log/exponential phase.
○ Larger biomass is produced by this method leading to higher
yield.
● Bioreactors − Large vessels in which large volumes (100 − 1000 litres) of culture
can be produced
○ Optimal growth conditions for microbes are present
(temperature, pH, substrate, salts, vitamins, etc.).
○
●
○ A bioreactor has the following components - agitator system,
oxygen delivery system, foam control system, temperature and
pH control system, sampling ports.
Downstream Processing
Bt Cotton
insulin chains.
● Separately produced chains A and B were extracted and combined by
creating a disulphide bond to form mature human insulin.
Gene Therapy
Molecular Diagnosis
a radioactive probe and autoradiography is used for detection. The regions where
mutation is present in the gene will not appear in the photographic film since
Transgenic Animals
through patenting. This new variety was actually derived by Indian farmers by
● Similarly Neem and turmeric, which have been used for ages in Indian
medicines, are also matters of dispute for patent rights.
Biopiracy
Abiotic Factors
● Some of the major abiotic factors that interact with the organisms are:
○ Temperature − It is the most relevant abiotic factor since all organisms
require an optimum temperature for their metabolism and other body
functions. Depending upon their ability to tolerate temperature range,
organisms are of two types- stenothermal (restricted to a narrow range of
temperature) and eurythermal (can tolerate a wide range of temperature).
○ Water − Water also is a major influencing factor. Life on earth is
impossible without water as it forms the major constituent of all living cells.
In oceans where quantity of water is not a limitation, the quality of water
becomes one. Depending upon the ability to tolerate salinity, organisms
can be stenohaline (restricted to narrow range of salinity) and euryhaline
(tolerant to wider range of salinity).
○ Soil − The nature and composition of soil differs from one place to
another depending upon the climate, weathering process, and soil
development method. The characteristic features such as soil
composition, grain size, percolation, water holding capacity, etc. determine
the native of the organisms it can support.
○ Light − The major source of light on earth is the Sun. Light is essential
for plants to perform photosynthesis. Certain plants become adapted to
perform photosynthesis under very low light since they are constantly
overshadowed by tall trees. Many plants also depend on light for their
flowering (photoperiodism). The availability of light on land is
comparatively higher than that in water.
Adaptations
● Adaptations are certain characteristics that organisms develop in order
to survive and reproduce better in their habitat.
● These adaptations can be physiological, behavioural, or morphological.
● Some of the adaptations are:
○ Desert plants have thick cuticle on their leaf surface and
stomata arranged in deep pits to reduce water loss. Their special
photosynthetic pathway CAM enables their stomata to remain
closed during day time. Their leaves are reduced to spines and
photosynthesis is carried out by flattened stems.
○ Animals of colder regions have shorter limbs and ears to
minimise heat loss (Allen’s rule) and the body is covered by thick
fur to reduce the heat loss. Their body has a thick layer of fat
(blubber) below their skin that acts as an insulator to minimise
heat loss.
○ People living in high altitudes have high RBC production and
increased breathing rates.
○ Some desert animals are capable of burrowing in order to
escape the heat. In addition, some desert animals such as
kangaroo rat are able to meet their water requirement through
internal fat oxidation. They also have ability to concentrate their
urine.
Population
● It is a group of similar individuals living in a geographical area, sharing
similar resources, and capable of interbreeding.
● Population has certain attributes, which individual organisms do not
possess:
○ Birth rate per capita births
○ Death rate per capita deaths
○ Sex ratio − Ratio of number of males to females in a population
● Age distribution
○ A population can be composed of individuals of different age
groups.
○ Age distribution plot for a given population is given by the age
pyramid.
○ The structure of the age pyramid determines the growth status
of the population, whether it is growing, stable, or declining.
Population Growth
● The size of a population is an ever-changing aspect since it depends
upon availability of food, predation, weather conditions, etc.
● This gives us an idea whether a certain population is growing or
declining.
● Some of the reasons for the increase or decrease in population:
○ Natality (B) − Number of births during a given period in the given
population
○ Mortality (D) − Number of deaths during a given period in the given
population
○ Immigration (I) − Number of individuals of the same species who have
come into the habitat from elsewhere during a given period
○ Emigration (E) − Number of individuals of the same species who have left
the habitat and gone elsewhere during a given period
● If N is the population at time t, then its density at t + 1 is
Nt + 1 = Nt + [(B + I) − (D + E)]
Growth Models
dN /dt = (b − d) × N
If (b − d) = r, then
dN/ dt = rN
Or,
Nt = N0 ert
Where,
Population Interactions
● A natural habitat consists of many organisms living together and these
organisms communicate and interact with each other. For example,
plants depend on insects for pollination.
● Interspecific interactions are interactions between two different species
of organisms. They can be either beneficial or harmful to one or both
partners.
Interspecific interactions
For example, lichens, interaction of algae and fungi, where both are
benefited
The fungi give support to the algae while the algae prepare the food for
the fungi.
Ecosystem
Structure of Ecosystem
Productivity
Decomposition
Energy Flow
● Sun is the sole source of energy for all ecosystems on the earth.
● Plants and other photosynthetic organisms utilize less than 50% of the
solar radiation known as the photosynthetically active radiation
(PAR).
● In an ecosystem, plants are called producers and all animals depend
upon the plants directly or indirectly for their food. Hence, they are
known as consumers or heterotrophs.
● The consumers can be further divided into primary consumers
(herbivores), secondary consumers (primary carnivores), and tertiary
consumers (secondary carnivores).
● Food chain − The energy flow among the various constituent animals is known
as the food chain.
● Food web − The interconnection of the various food chains is called the food
web.
● Trophic level − Every organism occupies a specific level in their food chain
known as the trophic level.
● Standing crop − Each trophic level contains a certain amount of living
material at a certain time known as the standing crop.
● The number of trophic levels in a food chain is restricted since the
energy transfer follows the 10 percent law i.e., only 10% of the energy
is transferred from a lower trophic level to a higher one.
Ecological Pyramids
● The energy relationship between the different trophic levels is
represented by the ecological pyramids.
● Their base represents the producers or the first trophic level while the
apex represents the tertiary or top level consumer.
● Ecological pyramids are of 3 types:
○ Pyramid of number
○ Pyramid of biomass
○ Pyramid of energy
● In most ecosystems, the three pyramids are upright except in some
cases:
○ The pyramid of biomass is inverted in an ocean ecosystem since
a small standing crop of phytoplankton supports a large number
of zooplankton.
○ The pyramid of number can be inverted when, say, a large tree
is eaten by small insects.
○ However, the pyramid of energy is always upright.
● A trophic level represents a functional level and not a single species as
such. Also, a single species may become a part of more than one
trophic level in the same ecosystem at the same time depending upon
the role it plays in the ecosystem.
● Limitations of ecological pyramids:
○ The ecological pyramids do not take into account the same
species belonging to more than one trophic level.
○ It assumes a simple food chain that almost never exists in
nature. It does not explain food webs.
○ Saprophytes are not given a place in ecological pyramids even
though they play a vital role in ecosystem.
Ecological Succession
● The composition of all ecosystems keeps on changing with change in
their environment. These changes finally lead to the climax
community.
● Climax community − It is the community which is in equilibrium with its
environment. Gradual and fairly predictable change in the species’ composition
of a given area is called ecological succession.
● Sere(s) − It is the sequence of communities that successively change in a
given environment. The transitional communities are called seral stages or seral
communities.
● Succession happens in areas where no life forms ever existed as in
bare rocks, cool lava, etc. (primary succession), or in areas which
have lost all life forms due to destructions and floods (secondary
succession).
● Primary succession takes hundreds to thousands of years as
developing soil on bare rocks is a slow process. Secondary succession
is faster than primary succession since the nature does not have to
start from scratch.
● During succession, any disturbances (natural/man-made) can convert
a particular seral stage to an earlier one.
● Hydrarch succession − It takes place in wet areas and converts hydric
conditions to mesic.
● Xerarch succession − It takes place in dry areas and converts xeric
conditions to mesic.
● Pioneer species − These are the species that first invade a bare area. On
land, these could be lichens that secrete enzymes to dissolve the rock surfaces
for soil formation while in water, pioneer species could be phytoplanktons.
● The ultimate result of all successions is a climax community, a mesic.
Nutrient Cycling
● The amount of nutrients present in the soil at a given time is known as
the standing state.
● Nutrients are never lost from the ecosystem. They are only recycled
from one state to another.
● The movement of nutrients through the various components of the
ecosystem is called nutrient cycling or biogeochemical cycles. They are
of two types:
○ Gaseous − Reservoir for these types of cycles exist in the atmosphere.
○ Sedimentary − Reservoir for these types of cycles exist in the earth’s
crust.
Carbon Cycle
Phosphorus Cycle
● Biodiversity occurs not only in the species level, but also in the
macromolecular levels.
● Biodiversity as described by Edward Wilson is the combined diversity
at all levels of biological organisation.
● The most important forms of biodiversity are:
○ Genetic diversity (diversity at the genetic level)
○ Species diversity (diversity at the species level)
○ Ecological diversity (diversity at the ecosystem level)
● There are close to 1.5 million plants and animals that have to be
discovered and described. More species have been discovered in
temperate regions as compared to tropics.
● According to an estimate made by Robert May, global species
biodiversity is about 7 million.
● Of the total species discovered so far, 70% are animals and 22% are
plants. Of the animals, 70% are insects.
● India has 2.4% of the world’s land and 8.1% of the total species
diversity. According to May’s estimate, 78% of the biodiversity is still
to be discovered.
● Applying this to India’s biodiversity figures, there still is a scope for
discovery of over 1 lakh species of plants and 3 lakh species of
animals.
Patterns of Biodiversity
● Latitudinal gradients − The plants and animals are not distributed evenly
worldwide. The diversity of living forms decreases as we go from the equator
towards the poles. A huge amount of plants and animals are concentrated in the
tropical region because of the following reasons.
● Tropical environment is less seasonal and almost constant and
predictable as compared to temperate environment.
● Tropics receive the major part of the solar energy, which contributes
to great productivity.
● Speciation is dependent upon time. Tropical areas have remained
undisturbed for millions of years unlike temperate regions, which have
experienced frequent glaciations in the past.
● Species-Area relationships − Alexander von Humboldt observed that
biodiversity increases with increase in explored area. This relationship can be
given by,
Where,
S = Species richness
A = Area
C = Y-intercept
Biodiversity Conservation
● Biodiversity conservation is necessary because of the following
reasons:
○ Many commercially important products are obtained by nature
such as food, fibre, wood, and countless industrial products.
○ Certain activities and products cannot be accomplished without
the help of nature such as production of oxygen and pollination.
○ Intangible benefits such as aesthetic pleasure are derived from
nature.
○ Conserving the species we share our planet with and passing the
rich legacy of biodiversity to our future generations is our ethical
duty.
● Biodiversity can be conserved by:
○ In-situ conservation - In order to conserve biodiversity better,
some of the world’s biodiversity hotspots (with high degree of
biodiversity and endemism) have been identified and are
protected. In India, biosphere reserves, wildlife sanctuaries, and
national parks are built for this purpose.
○ Ex-situ conservation - The threatened species of plants and
animals are taken out of their habitats and are kept in special
settings as in zoological parks, botanical gardens, and wildlife
parks.
Nowadays, the gametes of endangered species can be preserved
viable by methods such as cryopreservation and can be fertilized
in-vitro followed by propagation through tissue culture methods.
Similarly, seeds can be preserved in seed banks.
Environmental Issues
Pollution is the undesirable change brought about by chemical, particulate
matter, or biological materials to air, water, or soil.
Air Pollution
● Air is a complex, dynamic natural entity, which is essential for
supporting life on earth.
● Air pollutant is a substance that causes harm to the humans and other
living organisms.
● Some of the common pollutants of air:
○ Nitrogen dioxide
○ Sulphur dioxide
○ Carbon monoxide and carbon dioxide
○ Volatile organic compounds
○ Particulate matter
Greenhouse Effect
Water Pollution
● Water is very essential for the maintenance of life on earth.
● Due to human activities, water bodies have become polluted all over
the world.
● Some of the common pollutants and their sources are:
○ Domestic sewage − It mainly contains organic matter, which is
biodegradable. Microorganisms involved in their degradation consume a
lot of oxygen and the BOD of the water body increases leading to the
death of fishes and other aquatic life. Sewage also contains many
eliminates the cold water species and promotes the warm water species. In
the long run, it causes damage to the indigenous biodiversity of the water
body.
● Eutrophication
○ It is the ageing of a water body due to nutrient enrichment of its
water. It can be natural or artificial.
○ The natural process takes thousands of years, but due to human
activities, this process has got accelerated (accelerated/cultural
eutrophication).
○ Release of nutrient rich sewage and industrial effluents lead to
introduction of nutrients such as nitrogen and phosphorus and
increase in temperature and BOD of the water body, causing
increased biological activity, thereby leading to algal blooms.
This results in the loss of indigenous flora and fauna.
○ In some cases, large masses of floating plants (bog) develop,
finally converting the water body into land.
Solid Waste
● Consists of all the unwanted undesired materials thrown into the
dustbin
● It may be composed of biodegradable or non-biodegradable wastes.
● Open dumps used for disposing solid waste serves as breeding ground
for rats and flies. Therefore, sanitary landfills are used as a substitute
for these.
● Biodegradable wastes can be either aerobically on anaerobically
broken down using microbes. The non-biodegradable waste can be
recycled, reused, or dumped in landfills.
● Hospital wastes also contain hazardous materials, which have to be
disposed properly. Hospital wastes are generally incinerated.
● Irreparable computers and other electronic goods make up e-wastes,
which are either dumped in land fills or are incinerated. E-waste can
be recycled also to recover metals such as copper, iron, silicon, gold,
etc.
● To use the plastic waste in an efficient way, polyblend, a fine powder
of recycled modified plastic, has been developed. When polyblend is
mixed with bitumen, it can be used to lay roads with greater water
repellent capacity and greater life.
Radioactive Wastes
desertification.
Deforestation