3M Clean-Trace Training Webinar Handout

3M Food Safety
3M™ Clean-Trace™ ATP Technical Training

© 3M 2020. All Rights Reserved
Agenda
I. About 3M Food Safety
II. Technical training presentation
III. Question & Answer session
IV. Closing comments
© 3M 2020. All Rights Reserved 2
3M Food Safety Solutions

Quality Indicator Testing Pathogen Testing Allergen Testing
3M™ Petrifilm™ Plates 3M™ Molecular Detection System 3M™ Allergen Protein ELISA and Rapid Kits
Sample Collection Hygiene Monitoring UHT Beverage Testing
3M™ Sample Collection, Preparation and 3M™ Clean-Trace™ 3M™ Microbial Luminescence System
Processing Hygiene Monitoring and Management System
3
How to get started
➀ Go to 3M.com/foodsafety/education
➁ Create a login username and password.
➂ Follow the links to find the resources that fit your needs.
One-stop education.
It’s that easy.
3M Health Care Academy

SM
Taylor Lecy, 3M Food Safety
• U.S. Technical Services Representative at 3M Food

Safety
• Taylor uses her expertise in microbiology, R&D and food

safety to assist food processors with test method
implementation and advanced food safety applications
• Received a Bachelor of Science in Biology from the

University of Wisconsin-Madison and is a certified
FSPCA Preventive Controls Qualified Individual (PCQI)
3M Health Care Academy

SM
Key Learnings
Today you’ll learn about:
• Importance of hygiene monitoring and how ATP

detection technology works
• Building and executing a hygiene monitoring
program using the 3M Clean-Trace System
• Rapidly identifying and addressing problem areas
in your facility
• Best practices for reporting and data trending
• Support resources available for you and your
team
6
Now for a Poll!
What your biggest challenges with ATP
testing?
Select all that apply:
A. User training (e.g., swabbing
consistency)
B. Managing sampling plans
C. Manually recording data
D. Capability to analyze the data to make
improvements
E. Other
The Importance of Hygiene Monitoring
• Cleaning and sanitation programs are needed to prevent

pathogen and spoilage microorganisms as well as allergens in
food production facilities
• Hygiene monitoring is crucial to assess the effectiveness of

your cleaning and sanitation programs
• Hygiene monitoring is essential to support programs that

help to identify harborage sites for pathogens and spoilage
microorganisms
Hygiene monitoring generates data to determine if a surface has been cleaned sufficiently
and enables the high-risk decision to be made to start food production
Cleaning and Sanitation

Cleaning Sanitation
• Removal of debris • Reduction of microorganisms
• Improves efficacy of sanitizers to • If not combined with a cleaning
inactivate contaminants procedure, sanitation efficacy is reduced
• Should do the majority of the work and requires larger amounts of sanitizer
9
Why verify cleaning?
Just because it looks clean,

doesn’t mean it is clean
10
ATP as a Tool for Hygiene Monitoring

Phosphates
The 3M™ Clean-Trace™ Hygiene Monitoring and Adenosine

Management System rapidly verifies cleanliness
by measuring total ATP in environmental samples Adenosine Tri-Phosphate
collected from surfaces or water Molecule
11
ATP as a Tool for Hygiene Monitoring

Principle behind ATP bioluminescence.
Increase
in organisms
or organic
residues
ATP = Adenosine Triphosphate

The “energy currency” molecule
Increase
of all living organisms
in ATP levels
+
Luciferin/luciferase
Increase
in light (RLU)
12
Now for a Poll!
How do you verify cleaning and sanitation?
A. ATP detection
B. Microbiological tests
C. Visual inspection
D. We currently do not have a verification
program
13
How to verify that cleaning and sanitation

programs are working?
• Visual assessment is quick and it should be set as a minimum expectation; however, it is

highly a subjective approach
• Microbiological tests provide valuable quantitative information; however, do not enable
making fast decisions. Typically used for validation.
• ATP sees beyond the visual inspection and provides quantitative information that verify the
cleaning procedures are working
14
Implementing a Hygiene Monitoring Program
Risk-Based Validation Verification Information
Master list
Baseline Regular Track and
of test (How clean?) monitoring Trend
points
Risk-based approach to Determine Pass/Fail limits Verify effectiveness and Translate data into
select critical areas that and assign a quantitative determine if adjustment is information to know where
should be monitored value assigned to “clean” needed efforts needs to be directed
The 3M Clean-Trace System enables you to effectively

implement and maintain your hygiene monitoring program
15
Guidance for Constructing Sampling Plans
Map your facility: This is a team effort that requires expertise from different people in your plant
to help you to identify sites in your facility
• Focus in Zone 1 and Zone 2
• Identify 100-500 sites

• You will not test them every time,
but they should cover your facility
• You can randomize test points in the
3M Clean-Trace Software
Environmental sampling zones

Environmental Monitoring Handbook for the Food and Beverage Industries. 1st. Ed. 3M and Cornell University. 2019.
16
Guidance for Constructing Sampling Plans

How close to the food? (hazard) How hard is it to clean? (probability)
Zone 4: General Area Probability

Zone 3: Close Proximity L ow Medium High
Zone 2: Indirect contact
High
Hazard
Zone 1: Direct contact

Medium
L ow
High frequency testing
Once per week/month
Low frequency testing
Test point selection should be a risk-based approach

Environmental Monitoring Handbook for the Food and Beverage Industries. 1st. Ed. 3M and Cornell University. 2019.
17
Setting-Up Pass/Fail Limits

Start with a baseline
(suggested starting point is

150 RLU as pass, 300 RLU as fail)
Gather results after a good clean

from surfaces for a defined period of
time (e.g., 3 weeks)
Adjust pass/fail limits for those

surfaces using the data collected
Review on a regular basis (e.g.,

quarterly)
18
A Note About RLU Readings
• The 3M Clean Trace System does not produce “zero” RLU results
• Blank swabs will produce low RLUs levels
≤ 35 RLU for AQF100
≤ 20 RLU for AQT200
≤ 25 RLU for UXL100
• True reading is shown – the algorithm does not hide background RLUs
• Allows for greater ability to track and trend your results for different surfaces,
as it is not possible to see trends when your results are only 0 and 1
19
Components of the 3M™ Clean-Trace™

Hygiene Monitoring and Management System
ATP swabs Luminometer Software

• Collection device • Measures light output from ATP • Create and manage test
• Luciferin/luciferase reacts • Storage of test points points and sampling plan
with ATP to produce light • Storage of sampling plans • Data trending
• Generate reports
20
Now for a Poll!

Do you currently use the 3M Clean-Trace
System to verify cleaning and sanitation?
A. Yes, I utilize the 3M Clean-Trace System

B. No, I am utilizing a different ATP
detection system
C. No, I am not utilizing any ATP detection
system
21

22
3M™ Clean-Trace™ ATP Swabs Shelf life:
UXL100 Measures ATP from 10 months at 2-8oC

ATP Surface Swab solid surfaces 28 days at 21oC
100 swabs/case
Measures free residual

AQF100 ATP (non microbial)
ATP Free Water Swab 11 months at 2-8oC
100 swabs/case
from water samples
Ideal for CIP
Measures total ATP

AQT200 (microbial and non microbial) 10 months at 2-8oC
ATP Total Water Swab from water samples 60 days at 21oC
100 swabs/case
Ideal for CIP
• Maintain swabs in refrigeration for maximum shelf life • Verify expiration date in the package prior to use
• Keep swabs stored in foil pouch to protect against light exposure • Do not use the swabs past the expiration date
• Remove swabs from refrigerator 10 min before use • All swabs can withstand 3 freeze/thaw cycles
23
How to Swab Surfaces

Hold the swab between the thumb and
forefinger in such a way that the swab
can be rotated
1 2
Do not touch the swab near the swab bud
Apply downward
pressure
3 4
Flat surface: 10 cm x 10 cm / 4 in x 4 in
Irregular surface: Ensure good coverage
Rotate swab during sampling
Consistency is key!
24
Activate and Read 3M™ Clean-Trace™ Swabs
• Return swab to the tube after sample collection Place the swab immediately
• Press the handle down to activate into the luminometer to measure the
light production
• Shake side-to-side for at least 5 seconds
Results are reported in ~8-9
seconds
25
Swabbing UXL100 Dos and Don’ts

Do Don’t
 Apply pressure on swab & rotate ✘ Touch the swab or surface tested with hands
 Swab consistently on area sampled for test point ✘ Swab through puddles as extractant present
and method for collection in the swab tip will be washed away
 After activation and shaking side to side,
✘ Swab hot surfaces – let them cool and dry
measure swab immediately
 Remove used swabs from the instrument ✘ Lay swabs down after activating
 Place the Clean-Trace tests at room temperature

✘ Carry or store luminometer with used swab inside
for at least 10 minutes before use
 Can leave swab un-activated for
✘ Use expired swabs
up to 4 hours
26
Now for a Poll!

When do you typically perform your ATP
testing?
A. Before sanitizer application
B. After sanitizer application
C. N/A
27
Guidance for Swabbing
Best practice: Between the cleaning and sanitation step
When to swab? • Sanitizer does not work on a dirty surface
• Swabbing after cleaning reduces the risk of sanitizer interference
Use of swabs Swabs were designed for versatility because not all facilities
after sanitation have flexibility to swab in-between cleaning and sanitizing
• AOAC PTM showed that there was limited interference by residual
sanitizers (~200 ppm)
Verify for Because we cannot predict interactions with all sanitizers in

interference all situations, it is important to verify that the sanitizer you
use will not interfere with the ATP detection.
• Blank swabs dipped in diluted sanitizer (use same concentration) to look
for high results
• Dipped swabs in diluted sanitizer dosed with ATP to look for interference
with readings
28
Sample Collection for Water Swabs
• Collect desired water sample using a sterile cup

• Dip the water swab head into the sample
• Remove swab without swirling
• Activate and shake side to side for at least 5 seconds
• Place in instrument and measure immediately
29


30
3M™ Clean-Trace™ Luminometer LM1
Key Functions
• Contains all sampling plans and points
• Contains randomized sampling points
• Reads the amount of light emitted by the swab
• Stores Relative Light Unit (RLU) results until syncing with software
• Enable the recording of additional test types (visual inspection,
microbial, allergen)
• Displays picture of test point to enable sampling consistency
• Allows users to select pre-defined comments after a test
• Allows re-test if necessary
• Syncs with software using USB, Wi-Fi or Bluetooth
31
Best Practices for Luminometer Use

• Allow the LM1 to charge for 4 hours prior to use
• Check Date/Time prior to use (from the top of the screen swipe down
to be brought to the date and time settings)
• LM1 will enter sleep mode after 5 min and shutdown automatically after
2.5 hrs. Power down if not planning to use.
• Remember: the only way to wake the unit from sleep mode is by
pressing the up arrow for 2-3 seconds
• When you see a black screen, start by pressing the up arrow. If it
doesn’t wake, then press the power on bottom to power the unit
back on
• Keep the LM1 plugged in to a power source when not in use
• Note: If plugged in to PC, it only charges when the PC is fully
powered (not on standby or off)
32


33
3M™ Clean-Trace™ Hygiene Management Software
Constructing Sampling Plans and
Configuring Settings in the Software
Go to www.3M.com/ATPSupport for videos, other resources and direct support
34
Software Dashboard
Key Features
• Color coded for easy visualization of results
• Watch List enables rapid identification of
problem areas
• Global filters to focus on specific
plants/locations, date ranges, test types, and
sample plans
35
Test Point Randomization

Randomization helps you to:
• Control for biased sampling
• Ensure monitoring of all other sampling
points over period of time while you
keep an eye on critical points
36
Report Generation
37

Report Generation
• Simplify basic reporting and audit preparation
• Help user to quickly identify potential problem
areas and answering key questions
• Evaluates sanitation performance over time
and indicates where attention is needed
• Provides evidence for management to prove
the benefits of a hygiene monitoring program
38
Result Report Master Sanitation Schedule Rank Report
Result report from a defined selection Detailed view at a sample plan level Overview of most commonly failed
(time, test, location, etc) • 30-day calendar view test points for:
• Total number of test • Identify locations with potential issues • Trends
• Retests with comments • Visualize trends by a defined selection • First time failures
• Types of test (visual, ATP) (sample point, week) • Indication of improvement
• First clean success
39
Now for a Poll!
How often do you trend your ATP data?
A. Very often
B. Sometimes
C. Never
D. The system I use does not have data
trending capability
40
Trend Report
The value of data trending
41
Implementing a Hygiene Monitoring Program
Risk-Based Validation Verification Information
Master list
Baseline Regular Track and
of test (How clean?) monitoring Trend
points
Master sanitation ATP swabs, LM1 and Reporting tools and Software trending
plan is managed by software provides you synchronization help enables to identify trends
the software with the tools to generate to maintain up-to-date and thus direct the right
a robust baseline information about your resources to maintain an
monitoring program effective program
The 3M Clean-Trace System enables you to effectively

implement and maintain your hygiene monitoring program
42
3M™ Clean-Trace™ Hygiene Monitoring
and Management System
Received certification according to AOAC® Performance
TestedSM Methods program
• Matrices: Stainless steel, ceramic and plastic surfaces
• Variety of sources of ATP including foods as well as
relevant microorganisms
• Key conclusions:
• Minimal interference with common sanitizers used
in the food industry (~200 ppm)
• Detection of ATP from included sources of ATP
(food and microbial)
• Low variability in RLU values over time (up to 2 http://members.aoac.org/aoac_prod_imis/AOAC_Docs/
min) in a various conditions of temperature RI/20PTM/20C_041901_3MCleanTrace.pdf
43
Monitored
System
Go to www.3M.com/ATPSupport for You can also email directly to:

resources and direct support US: fsdcleantrace_us@mmm.com
OUS: fsdcleantrace_ous@mmm.com
44
Ask the
Experts
Taylor Lecy Dr. Gabriela Lopez Velasco
Live Q&A U.S. Technical Services Global Technical Services Specialist
3M Food Safety 3M Food Safety
45
Getting started has never been easier.
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Learn more at 3M.com/foodsafety/clean-trace

46
Thank you.
3M Food Safety
3M.com/foodsafety Taylor Lecy
U.S. Technical Services
Dr. Gabriela Lopez Velasco
Global Technical Services Specialist
3M Food Safety 3M Food Safety
47

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3M Food Safety

3M™ Clean-Trace™ ATP Technical Training

II. Technical training presentation

III. Question & Answer session

IV. Closing comments

© 3M 2020. All Rights Reserved 2

3M Food Safety Solutions

Sample Collection Hygiene Monitoring UHT Beverage Testing

© 3M 2020. All Rights Reserved 3

3M Health Care Academy

© 3M 2020. All Rights Reserved 4

Taylor Lecy, 3M Food Safety

• U.S. Technical Services Representative at 3M Food

• Taylor uses her expertise in microbiology, R&D and food

• Received a Bachelor of Science in Biology from the

3M Health Care Academy

© 3M 2020. All Rights Reserved 5

• Importance of hygiene monitoring and how ATP

© 3M 2020. All Rights Reserved 6

© 3M 2020. All Rights Reserved 7

The Importance of Hygiene Monitoring

• Cleaning and sanitation programs are needed to prevent

• Hygiene monitoring is crucial to assess the effectiveness of

• Hygiene monitoring is essential to support programs that

Cleaning and Sanitation

© 3M 2020. All Rights Reserved

Just because it looks clean,

© 3M 2020. All Rights Reserved

ATP as a Tool for Hygiene Monitoring

The 3M™ Clean-Trace™ Hygiene Monitoring and Adenosine

© 3M 2020. All Rights Reserved

ATP as a Tool for Hygiene Monitoring

ATP = Adenosine Triphosphate

© 3M 2020. All Rights Reserved 12

© 3M 2020. All Rights Reserved 13

How to verify that cleaning and sanitation

• Visual assessment is quick and it should be set as a minimum expectation; however, it is

© 3M 2020. All Rights Reserved 14

Implementing a Hygiene Monitoring Program

Risk-Based Validation Verification Information

The 3M Clean-Trace System enables you to effectively

© 3M 2020. All Rights Reserved

• Focus in Zone 1 and Zone 2

• Identify 100-500 sites

Environmental sampling zones

Guidance for Constructing Sampling Plans

Zone 4: General Area Probability

Zone 1: Direct contact

High frequency testing

Once per week/month

Low frequency testing

Test point selection should be a risk-based approach

© 3M 2020. All Rights Reserved 17

Setting-Up Pass/Fail Limits

(suggested starting point is

Gather results after a good clean

Adjust pass/fail limits for those

Review on a regular basis (e.g.,

© 3M 2020. All Rights Reserved 19

Components of the 3M™ Clean-Trace™

ATP swabs Luminometer Software

© 3M 2020. All Rights Reserved 20

Now for a Poll!

A. Yes, I utilize the 3M Clean-Trace System