VNR VIGNANA JYOTHI INSTITUTE OF ENGINEERING & TECHNOLOGY

B.Tech. IV Year I Semester – EIE - A

Analytical Instrumentation – 5EI10
Why am I Learning, What I am Learning
Roll.No Name Unit - 1
16071A1051 SWETHA.K ELECTROCHEMICAL INSTRUMENTS:
Basic concepts of analytical instrumentation,electro
16071A1052 THANVI REDDDY chemical instruments-pH meters, conductivity meters,
16071A1053 THOTTEMPUDI KAVYA dissolved oxygen analyzers, sodium analyzers, silica
analyzers.
16071A1054 AMRIT RAJ .T

16071A1055 LEELAVATHI .V
What did you learn from this unit
In this unit we learned the basic concepts related to analytical instrumentation,electro chemical instruments-
pH meters, conductivity meters and different analyzers like dissolved oxygen analyzer,sodium and silica.

Was the application illustrated, clear for understanding the topics covered in this unit
Yes, sir explained the concepts in a clear way with different applications.

Identify an application, to relate to the content learnt and explain

pH Meters are used to measure acidity levels in water & wastewater treatment, pharmaceuticals, chemicals &
petrochemicals, and other applications are known as pH meters. They are mostly used in food & beverages,
pharmaceuticals, mining, water treatment plants, and other industries.

An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. It has
multiple applications in research and engineering, with common usage in hydroponics, aquaculture,
aquaponics, and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.

A Dissolved Oxygen Meter is used to measure the amount of oxygen present in a unit volume of water; it
indicates if the water is useful for a specific application like water treatment plants, sewage treatment works,
river monitoring and fish farming.

A sodium analyzer is an on-line measurement system to ensure that water is not contaminated with sodium,
which can be corrosive and damaging in processes that require pure or ultrapure water. They are often used in
power plants for monitoring water cycle chemistry or in microelectronics manufacturing.

VNR VIGNANA JYOTHI INSTITUTE OF ENGINEERING & TECHNOLOGY

 B.Tech. IV Year I Semester – EIE - A
Analytical Instrumentation – 5EI10
Why am I Learning, What I am Learning
Roll.No Name Unit - 2
16071A1051 SWETHA.K
SPECTROPHOTOMETERS:
16071A1052 THANVI REDDDY Concepts of spectrophotometry,Beer-lambert’s law
and its derivation, uv-vispectrophotometers,IR
16071A1053 THOTTEMPUDI KAVYA spectrophotometers, FTIR , Flame photometers

16071A1054 AMRIT RAJ .T

16071A1055 LEELAVATHI .V
What did you learn from this unit
In this unit we learned the basic concepts related spectrophotometers , Beers-lambets law and its derivation,
UV-VIS spectrophotometers which is of single beam and double beam, IR spectrophotometers, Flame
spectrophotometers and its measurements.

Was the application illustrated, clear for understanding the topics covered in this unit
Yes, sir explained the concepts in a clear way with different applications.

Identify an application, to relate to the content learnt and explain

See the Light by Making a Cell Phone Spectrophotometer:

A spectrophotometer is a piece of lab equipment that measures the intensity of light. Scientists use this
expensive tool for a range of applications, including exploring how chemicals react, how quickly microorganisms
multiply, and how much protein or DNA is present in a sample.

With a cellphone and some specialty items like an LED and a simple diffraction grating slide, you can create
your own DIY apparatus to mimic the functionality of a spectrophotometer. Using your cellphone's camera to
record the light that passes through a solution of colored liquid, you can then use computer-based
spectrophotometry tools to analyze the images and evaluate light intensity.
 B.Tech. IV Year I Semester – EIE - A
Analytical Instrumentation – 5EI10
Why am I Learning, What I am Learning
Roll.No Name Unit - 2
16071A1051 SWETHA.K
GAS AND LIQUID CHROMATOGRAPHS:
16071A1052 THANVI REDDDY Chromatography-types-basic principles of gas
chromatography, liquid chromatography(HPLC)-
16071A1053 THOTTEMPUDI KAVYA different types of columns, detectors, recorders and
associated equipment for gas and liquid
16071A1054 AMRIT RAJ .T chromatographs and their applications, interpretation
and analysis..
16071A1055 LEELAVATHI .V
What did you learn from this unit
In this unit we learned the basic concepts related chromotographs and their principles and operations of
different types of devices that equipment uses that is detectors, recorders ,injectors and columns

Was the application illustrated, clear for understanding the topics covered in this unit
Yes, sir explained the concepts in a clear way with different applications.

Identify an application, to relate to the content learnt and explain

Gas Chromatography is used extensively in forensic science. Disciplines as diverse as solid drug dose (pre-
consumption form) identification and quantification, arson investigation, paint chip analysis, and toxicology
cases, employ GC to identify and quantify various biological specimens and crime-scene evidence.

HPLC has many applications in both laboratory and clinical science. It is a common technique used in
pharmaceutical development, as it is a dependable way to obtain and ensure product purity While HPLC can
produce extremely high quality (pure) products, it is not always the primary method used in the production of
bulk drug materials According to the European pharmacopoeia, HPLC is used in only 15.5% of
syntheses However, it plays a role in 44% of syntheses in the United States pharmacopoeia. This could possibly
be due to differences in monetary and time constraints, as HPLC on a large scale can be an expensive
technique. An increase in specificity, precision, and accuracy that occurs with HPLC unfortunately corresponds
to an increase in cost.