Beruflich Dokumente
Kultur Dokumente
Ms. Abbas
Overview:
In this lesson, students will be exploring an introduction to chromatography. Alongside the polarity
content they have been learning in class, students will be able to take a hands-on approach to learning
how mixtures can separate in a physical matter. Students will be working in groups of 3 or 4 and each
group will be assigned one marker dye. Students will then follow the procedures provided in a handout
and record observations for their marker dye. Students will then share their observations with the class
and have a whole class discussion.
Learning Objective(s):
Students will be able to explore the basics of chromatography in order to separate mixtures
Students will be able to make observations to connect keywords such as polar and nonpolar to the results
of marker dye separation
Students’ Interests and Identities (Explain how this lesson relates to students’ interest and identity; You
may choose to fill this in with a focal student in mind, or include more students in your class):
Interests Identities
• Students are able to work on a hands-on • Students are able to use text/drawings to
activity to explore chromatography discuss prior knowledge of key words
• Students are able to work in small groups such as mixture, solvent, solute, solvent,
to create their own observations regarding etc.
their marker dye separation
5E Instructional Framework
Identify which piece(s) of the 5E instructional framework you are targeting with this lesson plan and
provide your reasoning (1-2 sentences is fine).
Engage Students engage in a hands-on activity that allows them to be the scientists
Explore Students explore the newly introduced technique of chromatography (how it works)
Explain Students explain how the marker dye observations relate to mixtures, polar, and nonpolar concepts
Elaborate Students elaborate on their marker dye observations during small group and whole class discussion
Evaluate
Teacher will…
Teacher will…
Teacher will...
Chromatography Activity • Provide further instruction and set-up for chromatography activity
• Circulate room and assist students when needed
20-25 minutes
Teacher will...
Teacher will...
Debrief/Concluding Statements • Provide final thoughts and conclusions about the activity
• Mention how this is important for other scientific investigations
Remainder of time if remaining Students will...
A simple wrap-up of what we did, why we
• Provide any last thoughts or questions that may be present
did it, and how this can be used in the
science world for aspects beyond marker
dye separation
Notes:
- Try to pass out warm-up or activity when they come in (have students pick it up on the way in)
- Make sure the PowerPoint will open (correct format) before class begins
- Give students time to go through activity and write observations
Differentiation:
Most of the differentiation I am able to implement in this activity deals with the warm-up activity.
Students are able to “describe” each of the following in any way they want (text or drawing): mixture,
solute, solvent, solution. Students are then prompted to provide their ideas/predictions to discuss with the
class.
Assessment:
This can be done through an exit ticket summarizing the main take-away points that the activity guided
students towards. (I did not create an exit ticket because I knew we would be short on time). Ideally, this
would allow a teacher to know whether or not students understood the main points of the activity and its
relation to class content that had been introduced in lessons prior.
Chromatography Activity Warm-Up
Directions: Answer the following questions based on prior knowledge of the terms.
Pre-Activity Questions
1. What is Chromatography used for?
2. How does Chromatography work?
Materials
Rectangular piece of filter paper
250 mL beaker
Pencil
Ruler
Tape
Vinegar (1 mL)
Water
Markers ( Orange, Green, Brown, Black, Blue Purple)
Procedure
1. Use a pencil to draw a straight, horizontal line
about 1.5cm from one end of the piece of filter
Blue
paper. Write the color of your marker near the
top of the filter paper in pencil.
2. Use a marker to make a dot on the pencil line.
Allow the dot to dry and add more ink to the
same spot. Allow it to dry again and repeat this
process once more.
3. Pour 1mL vinegar into the beaker then add
water until the solution in the beaker is 50-60mL.
4. Place the filter paper into the beaker so the end with the ink spots is closest to the water
solution. Suspend the filter paper in the beaker from a pencil with a piece of tape so that
the end of the paper is in the solution but the dot is NOT (see diagram).
5. Allow time for the solution to travel up the paper. Remove the paper once the solution is
close to the top and allow time to dry (approximately 2 minutes).
6. Record your observations in the table below and repeat this procedure for each marker
color indicated.
Questions:
1. Indicate what colors appeared on the filter paper for each of the original dots? Record
this after you allow time for the solution to travel up the paper and have removed it to
dry.
Orange
Green
Brown
Black
Blue
Purple
2. Based on your observations of the chromatography results from your black ink spot, is
black ink a mixture? Explain; be sure to define the term mixture in your explanation.
3. Choose one of the colors above that is a mixture. Use the fact that the filter paper is
polar and the vinegar is less polar to describe the polarity of each of the components in
the mixture. (Hint: the most polar substance will be most strongly attracted to the
paper.)