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Introduction: -
Lignocaine:-
It is topically local anesthetic which blocks nerve conduction of sensory impulse from periphery.
Local aesthetic abolish sensation in limited area of the body without producing unconsciousness.
The myelinated fibers that conduct impulses for pain, temperature and autonomic activity are most
sensitive to action of local anesthetic.
Lignocaine is most frequently used. these compounds interacts with protein receptors of Na+ channels
to inhibit their activity.
Subject:
Human
Stopwatch.
Procedure:-
Mark the circle of an area that is free from blood vessels in forearms.
Result:-
Experiment:2
Subject:-
Rabbits
Apparatus:-
Lancet as needles, spirit lamp, test tube, syringes, stopwatch, capillary tube.
Chemicals:-
Heparin/EDTA as anti-coagulant.
Procedure:-
Take blood from ear vain of rabbit in both syringes with and without heparin.
Turn on stopwatch.
After every 1 min, break off the small piece of capillary tubes until a fibers thread appear.
Precautions
Result: -
In control tube blood will clot after 6-12
While in test sample , blood will take about 1 hour to clot due to the presence of anticoagulant