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TISSUE Typing
1. Micro cytotoxicity assay
2. Mixed lymphocyte culture (MLC)
3. Flow cyto cross typing
4. DNA analysis (genomic typing )
1-for patient
- HLA typing
- ABO typing
-Screening of serum for Abs reactive to donar HLA Ags
-Testing of serum for Auto-Abs
2-living related donor
- HLA typing
- Rh\ ABO typing
- cross matuing with patient serum to detect anti-donor Ab
2- Isograft
(between same identical twins )
3- Allograft
(homograft genetically different of the same species most
Common form organ )
4-xenograft
(from animal to human, baboon heart to infant, always rejecting, intense immune
response, very difficult to suppressor
Grafting : process of take cell , tissue or organ from one individual and placing
into another , it was discovered by marrium Webster.
Note :
- it also means implantation of non self tissue in body
- transplantation is 3rd area of immunopathology
- the first transplantation was in 1682
definition of death
- irreversible loss of capacity for consciousness and breath
- cardio respiratory death
- brain stem death
Classification of rejection:
3. effect of antibody
have role in HLC and complment will activate
my note : That mean the immune response against which is stronger i.e it more
strongly in bone marrow than the cornea
5. role of cytokine
2- accelerated rejection:
whiten a few day (2-5day) depend on cell mediated & on presence or
abcense of antibody
3- acute rejection:
from (7-21 day) depend on cell mediated & on presence or abcense of
antibody
4- chronic rejection:
latter than 3 month by cell mediated
5- immunological rejection:
than latter than 3 month