Quality Analysis of Amino Acids in Food

and Feedstuff:
The Biochrom AAA Solution.
Introduction

Challenges in food and feedstuff

analysis

•Analysis of complex sample matrix containing

carbohydrates, proteins, lipids, salts, amino acids,
vitamins, metal ions and minerals etc...
•Quantification of sulphur containing amino acids.
•Amino acids are a class of compounds with no specific
absorption wavelength – need derivatization.
•High concentration of salts after hydrolysis.
•Reproducibility in the results.
•Time of analysis
The Biochrom Solution

Analysis of complex sample matrices

• Minimal sample preparation required whilst

ensuring no matrix interference: only 2 simple
steps are required after the acid hydrolysis
process: dilution of the neutralised hydrolysate
in Loading Buffer and filtration prior to
injection. – FAST and CONVENIENT.
The Biochrom Solution

Quantification of sulphur containing

amino acids

•Full separation of oxidized forms of Cystine

(Cysteic acid) and Methionine (Methionine
sulfone) is possible after performic acid
oxidation of the sample.
•Determination of the quality of the oxidation
through the detection of the methionine
sulfoxides.
The Biochrom Solution

Class of compounds with no specific

absorption wavelength.
• Specific, automated post-column derivatization
of amino acids ensuring high selectivity and
reproducibility in any matrices, using two
wavelengths: 570nm and 440nm.
The Biochrom Solution

Reproducibility in the results.

• Optimum buffer formulation for the best

separation.
• Guaranteed retention time and peak area
reproducibility thanks to our ISO certified
ready-made buffers and reagents.
The Biochrom Solution

Time of analysis

• Accelerated Analysis of amino acids in 45min.

With the Sodium Accelerated Buffer System.
• More than 30 compounds separated whilst
maintaining excellent peak resolution, unlike
other faster methods.
The Instrumentation of Choice:
The Biochrom 30+

Fully automated
analysis of amino
acids in complex
• Ion Exchange Chromatography sample matrices.
• Stepwise elution of buffers
• Ninhydrin post-column
Principles derivatization.
• Spectrophotometric detection
at 570nm and 440nm.

• Autosampler Routine instrument

• Chromatographic unit
used worldwide - Dedicated analyzer
System Gold Standard for with specific
• PC/Monitor/Printer amino acid
configuration detection system
• Biosys & EZChrom Elite analysis.
Software
Biochrom 30+ Features and Benefits

Features Benefits
Cation exchange chromatography Ensures excellent peak resolution.

Ninhydrin post column Highly specific to amino acids,

derivatization ensuring very low interferences from
other analyte or the sample matrix.
Bench top unit Minimal footprint and easy access
for maintenance.
Ready-made chemistry Ensures high reproducibility in the
results and easy of use.
Fully developed methods No method development required.

Re-usable columns Cut the cost of ownership.

PEEK fittings and tubing Chemical inertness.

Fully automated system Can be used 24/7 in routine

environment.
Air cooled autosampler (optional) Ensures longer sample stability.

Large worldwide installed base References available.

Column technology

Features Benefits
Use of PEEK material and Inertness against strong
Sulfonated chemicals.
polystyrene/divinylbenzene
resin.
Accurate temperature Gradient programming
programming. available between 20-99oC
allowing accurate optimisation
of chromatography.
Analytical column suitable Long column life, up to 1200
for all complex matrices injections (on filtered samples)

Exclusive column cleaning Recyclable, reduced cost of

and repacking service ownership.
available.
Quality Buffers

Chemicals

• Manufactured under ISO 9001

standards.
• Guaranteed pH, Molarity and
retention time reproducibility
• Strict Quality Control of every
lots produced.
• Stable for up to 3 years
unopened.
• Exclusively formulated for
optimum separation on
Biochrom instruments.
Derivatization – Ninhydrin Reagent

Ultrosolve Plus Ultra Ninhydrin

Solution

Stability guaranteed for 3 years unopened.

When reagent made up, stable for 4 weeks.
Biosys control Software

Enables the control of all

the instrument parameters
• Flow rates
• Column temperature
• Reaction coil temperature
• Pressure monitoring
• Sample list creation

Automatic data acquisition

• Link with EZChrom Elite

EZChrom Elite Data Integration Software
Biochrom Support

Dedicated
AAA team

Reference Expertise in
instrument Amino acid
– The Gold Analysis for
Standard 30 years

Quality
product
Spare parts,
More than
consumable
500 units in
s and
operations
chemicals
worldwide
availability
Exclusive
Worldwide
Distribution
Network
Biochrom AAA configurations for
Food and feedstuff analysis
Sodium Accelerated Buffers System

Product
description
• Ideal for the analysis of
protein amino acids from
food and feedstuff
hydrolysates (oxidized or
non oxidized).
• Up to 30 compounds
separated and identified,
including Taurine, beta-
alanine, Gaba,
hydroxylysine, glucosamine
and galactosamine.
• Full amino acid profile
obtained in 46 min.
• Short methods available.
Protein Hydrolysate System

Product description

• Ideal for the analysis of

protein amino acids from
hydrolysates of synthetic
peptides.
• All 18 protein amino
acids identified and
separated.
• Uses only 3 buffers.
• Full amino acid profile
obtained in 45 min
• Short methods available
Summary

The Biochrom AAA solution

• Minimal sample preparation required

• Excellent peak resolution with baseline-free
artefacts
• Automated post-column derivatization
• Extensive knowledge database and methods
• Guaranteed retention time and peak area
reproducibility
• Technical expertise
• Robust technique – The Gold Standard
Integrated solution
Fully
automated
routine
instrument Compact
Technical
bench top
Support
unit

Cation
Complete
Exchange
AAA Perfect Chromatog
solution analytical raphy
results.
Long Term
Reliability

Highly
Wide
specific
selection of
Ninhydrin
application
detection
notes
system
PEEK
Biochrom
tubing
expertise
and
for more
Ceramic
than 30
pump
years
heads