of the papers used in cigarette manufacture, as well

as the filter material. 3 Toluene

#75a

Pyrolysis-GC/MS of Tobacco 4. with Menthol

Limonene
In this example, a small (~250 µG) sample of the 5 Phenol
tobacco from a mentholated cigarette was pyro-
Application Note 6 Glycerine
lyzed at 700°C for 15 seconds. The resulting
Tobacco
pyrogram shows a wide range of natural products, 7 Menthol
inclThe
udingmaterial
nicotine and
usedlevogl ucosan, whiisch ais carefully
in cigarettes a 8
formulated Nicotine of natural to-
product
product of cellulose, as well as additves, includ-
bacco together with a variety of additives. Analytical pyrolysis can reveal both
the materials produced from the tobacco at high 9 temperatures
Levoglucosan
as well as the
Author: ing glycerine and menthol. Selected peaks from
volatile additives. Pyrolysis has also been applied to the study of the papers
used in cigarette manufacture,as well as the filter material.
T. Wampler the pyrogram are identified in Table I.
In this example, a small (~250 µG) sample of the tobacco from a mentholated
cigarette was pyrolyzed at 700°C for 15 seconds. The resulting pyrogram shows
a wide range of natural products, including nicotine and levoglucosan, which
is a product of cellulose, as well as additves, including glycerine and menthol.
Selected peaks from the pyrogram are identified in Table I.
A bundanc e

T IC : [B S B 1 ]T O B A C 0 4 .D
1300000

1200000

8
1100000

1000000 7
Table I
900000

Peak number Compound

800000

1 Acetic acid 700000

2 Propylene glycol
3 Toluene 600000
4. Limonene
5 Phenol 500000

6 Glycerine
400000
7 Menthol
4
8 Nicotine 300000
9 Levoglucosan 1 5
200000
3 6
2
9
100000

0
5 .0 0 1 0 .0 0 1 5 .0 0 2 0 .0 0 2 5 .0 0 3 0 .0 0 3 5 .0 0
T im e -->

Figure 1. Tobacco with Menthol

Instrument Conditions

Pyroprobe Autosampler

Valve Oven: 300°C

Pyrolysis: 700°C
Time: 15 seconds

GC/MS

Column: 35% phenyl (30m x 0.25mm x .25mm)

Carrier: Helium, 50:1 split
Injector: 300°C
Oven: 40°C for 2 minutes
8°C/min to 300°C hold 12 minutes

FOR MORE INFORMATION CONCERNING THIS APPLICATION,

WE RECOMMEND THE FOLLOWING READING:

W. S. Schlotzhauer and O. T. Chortyk, Recent Advances in Studies on the Pyrosynthesis of Cigarette Smoke Constituents, J.
Anal. Appl. Pyrolysis, 12, (1987) 193.

CDS Analytical, 465 Limestone Road, P.O. Box 277, Oxford, PA USA 19363-0277 PH: 610 932 3636 FX: 610 932 4158