Beruflich Dokumente
Kultur Dokumente
Catalysis
1
Tuesday, March 10, 2015
Enzyme Kinematics Powerful catalysts
-deterine by rate of disappearance of - Proximity effect - enzyme bring S to
reactants or rate of appearance of products ACTIVE SITE
[E] = k -> conc. of enzyme constant - Orientation effect- E brings S at right
distance for reaction to occur
[S] -> conc. of substrate vary
Michaelis-Menten Approach to Enzyme
Kinematics - Energy Effect - lower th energy barrier by
inducing strain in substance
- model for non allosteric enzymes
- E + S -> ES -> E + P • induce strain -> unstable -> more
reactive
- rate depends on substrate concentrations - Catalytic effect- provide grps (acidic,
- V= Vmax [S]/ Km + [S] basic, etc) required for reaction
2
Tuesday, March 10, 2015
• resembles susbstrate (substrate
analogs)
• increase substrate concentration to
avoid competition
• Vmax - same (intersect @ y-axis);
• Km & slope - increase
- non competitive
• substrate to other site, inhibitor to other
• cause conformational change affects
active site
• hard to remove inhibitor
• Vmax -decrease
• Km is same (intersect @ X-axis)
• slope- increases
- Uncompetitive inihibitor
• Vmax & Km decreases
• Slope - same