CHINESE GENERAL HOSPITAL COLLEGE OF NURSING AND LIBERAL ARTS

JOURNAL SUMMARY and NURSING IMPLICATION

Cancer is one of the leading causes of death worldwide. And because of this,
biomarkers have been advanced nowadays, even innovations take place in diagnosing
precancerous tumors and severity of the case. The usual tumor markers like Carcinoembryonic
antigen, a-fetoprotein, prostate-specific antigen are low sensitive. Same as through with Mass
Spectrometry (MS), Liquid Chromatography (LC) ---has also limitations and maybe high risk for
low identification for possible tumor or precancerous tissues. And with the advancement,
discovery of innovative set of Proteomic technologies appeared ---

2D difference Gel Electrophoresis, this kind of study is having a cyanine solution

(flurophores) for which it serve as the protein markers and mixing it with a specific protein
sample together and placing it on a single gel and this indicates the initiation of fluoroscanning.
And one of its advantages are accessibility of usage and very high sensitivity to protein marking
process.

Next one is the MALDI Imaging Mass Spectrometry,is a kind of advanced proteomic
technology where the actual tissue is put on a matrix for some tests on the imaging mass
spectrometry, it is also convenient for dual tests like having a protein impression and a
histopathology impressions at the same time. Aside from mass spectra, many analyses can be
made like background noise subtraction, spectral normalization and spectral alignment.

The third one is the Electron Transfer Dissociation MS, it is an analysis of peptides
through the use of mass spectrometry, with the identification of loss of phosphoric acid and N-
linked glycans which Phosphorylation and glycosylation indicates the development of cancer
cells/ precancerous tissues.

And the last one is the Reverse-Phase Protein Assay, which have the principle of
spotting of the patient samples--- proteins and tissues in an assay with a nitrocellulose support.
It traces pathways in due presence of developing cancers, it also validate cellular pathways in
mathematical models and identification of Cancer antigens that are capable of identifying an
antibody response in patients with cancer.

NURSING IMPLICATION:
Advancements and latest technologies are booming rapidly. Healthcare has its own
innovations that would make care to the patients and diagnosis convenient and accurate wise.

Nurses should be updated with these technologies and that we are dealing today.
Reading latest journals regularly, doing researches, and attending health related seminars
would make selves informed and on to date.
 JOURNAL
“ADVANCED PROTEOMIC
TECHNOLOGIES FOR CANCER BIOMARKER
DISCOVERY”

January 18, 2011

SUBMITTED BY:
QUINTO, JEMMA HANNA P.
GROUP 4, III-D