Beruflich Dokumente
Kultur Dokumente
Packs
by W J. LINGHORNE, D.D.S., Department of Physiology
and
D. C. O'CONNELL, M.S.A., Banting and Best Department of Medical Research,
University of Toronto
pack+ penicillin
sulphathiazole
duplicate according to the method described
above, with the swab stick.
The pack was placed in broth, and
pack+
Pack
incubated for 24 hours, then removed and placed
in the next broth tube and incubated for 1 hour.
Following this, it was transferred to another tube Micrococcus
and incubated for another 24 hours. The cataruhalis 7, 7, 14, 9, 7, 8,
procedure was continued each day. Day by day Streptococcus
the tubes from which the pack had been removed viridans 10, 10, 10, 10, 11, 10,
were seeded with staphylococcus organisms and Streptococcus
incubated for 24 hours. Up to the 35th day, no hemolyticus . 7, 7, 7, 8, 10,11,
growth occurred in either the 1 hour or the 24 Staphylococcus
hour tubes. This same pack in broth was set aside aureus 16, 17, 8, 10, 16, 14,
in the laboratory for one year, during which time Psuedomonas
the broth had evaporated to dryness. When the aeruginosa 2, 1, 1, 2, 1, 2,
pack was placed in broth for 1 hour, once again In addition to the tests carried out against
sufficient of the bacteriostatic agent had diffused the above pathogenic bacteria, a similar test was
from the pack to prevent the growth of made against candida albicans, the micro-
staphylococcus aureus. organism concerned in thrush. The zone of
The following studies were made of the inhibition produced against C, albicans was 20
bacteriostatic effect of the pack against other mm, indicating that the pack has considerable
organisms. Using the agar-plate-with-well fungicidal properties.
technique (see Appendix III) the effect of the IN VIVO STUDIES
pack against pseudomonas aeruginosa, mi- Studies were carried out to investigate the
crococcus catarrhalis, staphylococcus aureus, effect of packing periodontal pockets in humans
streptococcus viridans and streptococcus and dogs.
hemolyticus, was determined. Procedure
In addition, a comparison of the bac- Material taken from the depths of the
teriostatic effect of the pack with that of pack + periodontal pockets was placed on slides, care
sulphathiazole and pack + penicillin was made. being taken to prevent maceration of the
The following chart gives the results of organisms and it was allowed to dry in air. After
the testing of the pack alone, pack + drying, the smears were examined under oil, at
sulphathiazole and pack + penicillin. least 30 fields being considered in thin
Test materials: preparation.
(1) Pack Three methods for removing the material
(2) Pack + 5% sulphathiazole powder from the pocket were investigated: (1) using a
suitably shaped wooden toothpick, (2) a metal
probe and (3) a capillary tube pipette. The first pack. The pack was again removed in 24 or 48
was the method selected and used throughout hours. In a limited number of cases the pack was
these experiments. In each case the pocket area allowed to remain in place for 4 to 7 days.
was isolated with cotton rolls, excess moisture
re-moved by blast of air, the toothpick was Method II
suitably shaped and lightly scraped along the Where the pocket was narrow, tor-
base of the pocket.
For practical purposes the microscopic findings
were divided into three groupings. Those smears
in which no