Sensory Evaluation of Food

FOOD SCIENCE AND TECHNOLOGY

A Series of Monographs, Textbooks, and Reference Books

EDITORIAL BOARD

O w e n R. Fennema University of Wisconsin-Madison

Marcus Karel Massachusetts lnstitute of Technology
Gary W. Sanderson Universal Foods Corporation
Steven R. Tannenbaum Massachusetts lnstitute of Technology
Pieter Walstra Wageningen Agricultural University
J o h n R. Whitaker University of California - Davis

1. Flavor Research: Principles and Techniques, R. Teranishi, I. Hornstein,

P. Issenberg, and E. L. Wick
2. Principles of Enzymology for the Food Sciences, John R. Whitaker
3 . Low-Temperature Preservation of Foods and Living Matter, Owen R.
Fennema, Wiliam 0. Powrie, and Elmer H. Marth
4. Principles of Food Science
Part I: Food Chemistry, edited by Owen R. Fennema
Part II: Physical Methods of Food Preservation, Marcus Karel, Owen R.
Fennema, and Daryl B. f u n d
5. Food Emulsions, edited by Stig E. Friberg
6 . Nutritional and Safety Aspects of Food Processing, edited by Steven R.
Tannenbaum
7 . Flavor Research: Recent Advances, edited by R. Teranishi, Robert A.
Flath, and Hiroshi Sugisawa
8 . Computer-Aided Techniques in Food Technology, edited b y IsraelSaguy
9 . Handbook of Tropical Foods, edited by Harvey T. Chan
10. Antimicrobials in Foods, edited by Alfred Larry Branen and P. Michael
Davidson
1 1. Food Constituentsand Food Residues: Their Chromatographic Determi-
nation, edited by James F. Lawrence
12. Aspartame: Physiology and Biochemistry, edited by Lewis D. Stegink
and L. J. Filer, Jr.
13. Handbook of Vitamins: Nutritional, Biochemical, and Clinical Aspects,
edited by Lawrence J. Machlin
14. Starch Conversion Technology, edited by G. M. A. van Beynum and J.
A . Roels
15. Food Chemistry: Second Edition, Revised and Expanded, edited by
Owen R. Fennema
16. Sensory Evaluation of Food: Statistical Methods and Procedures, Mi-
chael O'Mahon y
17. Alternative Sweetners, edited by Lyn O'Brien Nabors and Robert C.
Gelardi
18. Citrus Fruits and Their Products: Analysis and Technology, S. V. Ting
and Russell L. Rouseff
19. Engineering Properties of Foods, edited by M. A. Rao and S. S. H. Rizvi
20. Umami: A Basic Taste, edited by Yojiro Kawamura and Morley R. Kare
21. Food Biotechnology, edited by Dietrich Knorr
22. Food Texture: Instrumental and Sensory Measurement, edited by How-
ard R. Moskowitz
23. Seafoods and Fish Oils in Human Health and Disease, John E. Kinsella
24. Postharvest Physiology of Vegetables, edited by J. Weichmann
25. Handbook of Dietary Fiber: An Applied Approach, Mark L. Dreher
26. Food Toxicology, Parts A and B, Jose M. Concon
27. Modern Carbohydrate Chemistry, Roger W. Binkley
28. Trace Minerals in Foods, edited by Kenneth 7. Smith
29. Protein Quality and the Effects of Processing, editedby R. Dixon Phillips
and John W. Finley
30. Adulteration of Fruit Juice Beverages, edited by Steven Nagy, John A.
Attawa y, and Martha E. Rhodes
31. Foodborne Bacterial Pathogens, edited by Michael P. Doyle
32. Legumes: Chemistry, Technology, and Human Nutrition, editedby Ruth
H. Matthews
33. Industrialization of Indigenous Fermented Foods, edited by Keith H.
Steinkraus
34. International Food Regulation Handbook: Policy Science Law, edited
by Roger 0. Middlekauff and Philippe Shubik
35. Food Additives, edited by A. Larry Branen, P. Michael Davidson, and
Seppo Salminen
36. Safety of Irradiated Foods, J. F. Diehl
37. Omega-3 Fatty Acids in Health and Disease, edited by Robert S. Lees
and Marcus Karel
38. Food Emulsions: Second Edition, Revised and Expanded, editedby Kdre
Larsson and Stig E. Friberg
39. Seafood: Effects of Technology on Nutrition, George M. Pigott and
Barbee W. Tucker
40. Handbook of Vitamins: Second Edition, Revised and Expanded, edited
by Lawrence J. Machlin
41. Handbook of Cereal Science and Technology, Klaus J. Lorenz andKarel
Kulp
42. Food Processing Operations and Scale-Up, Kenneth J. Valentas, Leon
Levine, and J. Peter Clark
43. Fish Quality Control by Computer Vision, edited by L. F. Pau and R.
Olafsson
44. Volatile Compounds in Foods and Beverages, edited by Henk Maarse
45. Instrumental Methods for Quality Assurance in Foods, edited by Daniel
Y. C. Fung and Richard F. Matthews
46. Listeria, Listeriosis, and Food Safety, Elliot T. Ryser and Elmer H. Marth
47. Acesulfame-K, edited by 0. G. Ma yer and F. H. Kernper
48. Alternative Sweeteners: Second Edition, Revised and Expanded, edited
by L yn 0'Brien Nabors and Robert C. Gelardi
49. Food Extrusion Science and Technology, editedby Jozef L. Kokini, Chi-
Tang Ho, and Mukund V. Karwe
50. Surimi Technology, edited by Tyre C. Lanier and Chong M. Lee
51. Handbook of Food Engineering, edited b y Dennis R. Heldman and Daryl
8. Lund
52. Food Analysis by HPLC, edited by Leo M. L. Nollet
53. Fatty Acids in Foods and Their Health Implications, edited by Ching
Kuang Chow
54. Clostridiurn botulinum: Ecology and Control in Foods, edited b y Andreas
H. W. Hauschild and Karen L. Dodds
55. Cereals in Breadmaking: A Molecular Colloidal Approach, Anne-Char-
lotte Eliasson and KJre Larsson
56. Low-Calorie Foods Handbook, edited by Aaron M. Altschul
57. Antimicrobials in Foods: Second Edition, Revised and Expanded, edited
by P. Michael Davidson and Alfred Larry Branen
58. Lactic Acid Bacteria, edited by Seppo Salrninen and Atte von Wright
59. Rice Science and Technology, edited b y Wayne E. Marshall and James
1. Wadsworth
60. Food Biosensor Analysis, edited by Gabriele Wagner and George G.
Guilbault
61. Principles of Enzymology for the Food Sciences: Second Edition, John
R. Whitaker
62. Carbohydrate Polyesters as Fat Substitutes, edited by Casirnir C. Akoh
and Barry G. Swanson

Additional Volumes in Preparation

Engineering Properties of Foods: Second Edition, Revised and Ex-

panded, edited by M. A. Rao and Syed S. H. Rizvi

Handbook of Brewing, edited by William A. Hardwick

 Sensory

of Food
Statistical Methods
and Procedures

Michael O'Mahony

Taylor & Francis

Taylor &Francis Croup

Boca Raton London New York Singapore

A CRC title, part of the Taylor & Francis imprint, a member of the
Taylor & Francis Group, the academic division of T&F lnforma plc.
Library of Congress Cataloging-inPublication Data

O'Mahony, Michael, [date]

Sensory evaluation of food.
(Food science and technology ; 16)
Includes index.
1. Food-Sensory evaluation-Statistical methods.
I. Title. 11. Series: Food science and technology
(Marcel Dekker, Inc.) ; 16.
TX546.043 1985 664'.07 85-16090
ISBN 0-8247-7337-3

COPYRIGHT O 1986 by MARCEL DEKKER, INC. ALL RIGHTS RESERVED

Neither this book nor any part may be reproduced or transmitted in any form
or by any means, electronic or mechanical, including photocopying, micro-
filming, and recording, or by any information storage and retrieval system,
without permission in writing from the publisher.
MARCEL DEKKER, INC .
270 Madison Avenue, New York, New York 10016
To Eileen
 Preface

The aim of this book is to provide basic knowledge of the logic and computation
of statistics for the sensory evaluation of food, or for other forms of sensory
measurement encountered in, say, psychophysics. All that is required is that
the reader be able to add, subtract, multiply, and divide.
The book is aimed at three types of reader: first, the undergraduate or
graduate student who needs some statistics for a research project or who is
simply trying to fulfill the requirements for a degree before escaping academia;
second, the unwitting food scientist who has just been told that he or she is
now in charge of sensory evaluation, despite having had no training, and needs
to catch up quickly; and third, the established sensory professional who wants
a ready reference, some guidance, and an instant introduction to some new
tricks.
The book will take you from "ground zero" into the traditional realms of
statistics and experimental design. It will lead you there gently, while concen-
trating on the logic behind the tests. It will look at some of the special diffi-
culties involved in applying statistics to sensory data and will also give you a
good, healthy dose of nonparametric statistics.
The book is hard-headed, but it is also very simple to understand. This is
because it is written by a very simple nonstatistician. If I can understand it,
you can too!

Michael O'Mahony
 Contents

Preface V

Key to Statistical Tables xiv

1 BEFORE YOU BEGIN

1.1 How to Use This Book

1.2 The Use of Statistics in Sensory Evaluation Versus Its Use in
Psychophysics, Experimental Psychology, and Consumer
Testing
1.3 Why Use Statistics Anyway?
1.4 Some Perils of Sampling

2 AVERAGES, RANGES,AND THE NATURE OF NUMBERS

2.1 What Is the Average? Means, Medians, and Modes

2.2 Which Measure of Central Tendency Should You Use?
2.3 How are Numbers Spread? Range, Interquartile Range,
Mean Deviation
2.4 Variance and Standard Deviation
viii Contents

2.5 Worked Example: Find the Mean, Standard Deviation, and

Variance
2.6 The Nature of Numbers: Types of Scale
2.7 A First Look at Parametric and Nonparametric Statistical Tests

3 PROBABILITY, SORTING, A N D ARRANGING

What Is Probability?
How to Compute a Probability: The Total Number of Events
The Multiplication Law of Probability: Getting Two Heads
and One Tail
The Addition Law of Probability: Getting a Red or a White Ball
Worked Examples: Lots of Probability Calculations
Sorting
Permutations and Combinations
Worked Examples: Sorting, Permutations, and Combinations

4 NORMAL (GAUSSIAN) DISTRIBUTION: z TESTS

4.1 What Is a Normal Distribution?

4.2 z Scores
4.3 z Tests: Computing Probabilities
4.4 When Are Normal (Gaussian) Distributions Used?
4.5 How Normal (Gaussian) Distributions Are Used in Statistical
Theory: Sampling Distributions, Standard Errors, and the
Central Limit Theorem
4.6 Worked Example: z Tests-Computing Probabilities from
Normal Distributions

5 THE BINOMIAL TEST: APPLICATIONS I N SENSORY

DIFFERENCE A N D PREFERENCE TESTING

5.1 How to Compute Probabilities Using the Binomial Expansion

5.2 The Binomial Test and the Null Hypothesis
5.3 Levels of Significance: Type I and Type I1 Errors
5.4 One- and Two-Tailed Tests
5.5 Why the Binomial Test Is Nonparametric
5.6 Large Samples: When the Binomial Expansion A.pproximates to
a Normal Distribution
5.7 The Poisson Distribution
Contents

5.8 The Sign Test. One- and Two-Sample Difference Tests:

Related- and IndependentSamples Design
5.9 Using the Binomial Test to Compare Two Proportions:
A Two-Sample Binomial Test
5.10 Brief Recapitulation on Design for the Sign Test and the
Binomial Comparison of Proportions
5.1 1 Application of Binomial Statistics to Sensory Difference and
Preference Testing
5.12 Assumptions: Use and Abuse of Binomial Statistics in
Sensory Testing
5.1 3 Worked Examples: Some Binomial Tests
5.14 Final Example: What is a Flavor Difference? Some Thoughts
on the Philosophy of Difference Testing

6 CHI-SQUARE

What Is ChiSquare?
ChiSquare: Single-Sample Test-One-way Classification
ChiSquare: Two-Way Classification
Assumptions and When to Use ChiSquare
Special Case of Chi-Square: The 2 X 2 Table
Further Note on the ChiSquare Distribution
The McNemar Test
The Cochran Q Test
The Contingency Coefficient
Worked Examples: Chi-Square Tests

7 STUDENT'S t TEST

What Is a t Test?
The One-Sample t Test: Computation
Worked Example: One-Sample t Test
The Two-Sample t Test, Related Samples: Computation
Worked Example: TwoSample t Test, Related Samples
The Two-Sample t Test, Independent Samples: Computation
Worked Example: TwoSample t Test, Independent Samples
Assumptions and When to Use the t Test
The Independent-Samples t Test with Unequal Variances
Further Statistical Theory: The Relationship Between t
Distributions and Normal Distributions, t Tests and z Tests
Can You Use t Tests to Analyze Scaling Data?
Additional Worked Examples: t Tests
X Contents

8 INTRODUCTION TO ANALYSIS OF VARIANCE AND THE

ONE-FACTOR COMPLETELY RANDOMIZED DESIGN 135

Why You Don't Use Multiple t Tests 135

Logic Behind Analysis of Variance and the F Ratio 136
How to Compute F 140
Computational Formulas for Sums of Squares and Degrees of
Freedom 143
Computational Summary for the One-Factor, Completely
Randomized Design 145
Worked Example: One-Factor, Completely Randomized
Design 147
Computation When Sample Sizes Are Unequal 149
Is Analysis of Variance a One- or a Two-Tailed Test? 150
How F Is Related to t 151
Assumptions and When to Use Analysis of Variance 151
Can You Use Analysis of Variance for Scaling Data? 152

9 MULTIPLE COMPARlSONS 153

Logic Behind Multiple Comparisons

Various Multiple-Comparison Tests: Formulas for
Calculating Ranges
Which Multiple-Comparison Test Should You Use?
How to Compute Fisher's LSD Test
Worked Example: Fisher's LSD Test
How to Compute Duncan's Multiple-Range Test
Worked Example: Duncan's Multiple-Range Test
Additional Worked Example: A Completely Randomized
Design with Multiple Comparisons

10 ANALYSIS OF VARIANCE: TWO-FACTOR DESIGN WITHOUT

INTERACTION, REPEATED MEASURES 171

10.1 Logic Behind the Two-Factor Design Without Interaction,

Repeated Measures 171
10.2 Computational Summary for the Two-Factor Design Without
Interaction, Repeated Measures 173
10.3 Worked Example: Two-Factor Design Without Interaction,
Repeated Measures 176
Contents

10.4 Assumptions and When to Use the Two-Factor Analysis of

Variance Without Interaction
10.5 A Further Look at the Two-Factor ANOVA: The Completely
Randomized Design
10.6 Additional Worked Example: Two-Factor Design Without
Interaction, Repeated Measures

11 ANALYSIS OF VARIANCE: TWO-FACTOR DESIGN WITH

INTERACTION

What Is Interaction?
Interpretation of Interaction and Between-Treatments
F Values
How to Compute a Two-Factor ANOVA with Interaction
Worked Example: Two-Factor ANOVA with Interaction
Assumptions and When to Use the Two-Factor Design with
Interaction
What if the Interaction Is Not Significant?
Sometimes You Cannot Calculate Interaction Mean Squares
A Cautionary Tale
Additional Worked Example: Two-Factor Analysis of
Variance with Interaction

12 ANALYSIS OF VARIANCE: THREE-AND FOUR-FACTOR

DESIGNS

12.1 How to Compute a Three-Factor ANOVA

12.2 How to Interpret Two- and Three-Way Interactions
12.3 Sometimes You Cannot Compute Higher-Order Interactions
12.4 How to Compute a Four-Factor ANOVA
12.5 Worked Examples: Computation and Interpretation of
ANOVA with Two or More Factors

13 FIXED-ANDRANDOM-EFFECTSMODELS

13.1 What Are Fixed- and Random-Effects Models?

13.2 Theory Behind Fixed- and Random-Effects Models
13.3 Summary of the Denominators for F Ratios for Fixed
and Random Effects
 Contents

14 SPLIT-PLOT DESIGN

14.1 How the Split-Plot Design Is Related to Other Designs

14.2 Logic of the Split-Plot Design
14.3 Split-Plot Computation Scheme
14.4 Worked Example: Split-Plot Analysis of Variance

15 CORRELATION AND REGRESSION

Some Preliminaries
What Are Correlation Coefficients?
How to Compute Pearson's Product-Moment Correlation
Coefficient
Worked Example: Pearson's Product-Moment Correlation
Is the Correlation Significant?
The Coefficient of Determination
Assumptions and When to Use the Correlation Coefficient
Correlation Does Not Imply Causality
Further Topics for Correlation: Means, Differences, and
Significance
Logic of Linear Regression
Computation for Linear Regression
Worked Example: Linear Regression
Assumptions and When to Use Linear Regression
Worked Example: Correlation and Linear Regression

16 ADDITIONAL NONPARAMETRIC TESTS

When to Use Nonparametric Tests

The Wilcoxon Test: A Two-Sample, Related-Samples
Difference Test
Worked Example: The Wilcoxon Test
The Mann-Whitney U Test: A Two-Sample, Independent-
Samples Difference Test
Worked Example: The Mann-Whitney U Test
The Wilcoxon-Mann-Whitney Rank Sums Test
Worked Example: The Wilcoxon-Mann-Whitney Rank
Sums Test
Spearman's Ranked Correlation
Worked Example: Spearman's Ranked Correlation
The Friedman Two-Factor Ranked Analysis of Variance
(Two-Factor Repeated-Measures Design)
Worked Example: The Friedman Test
Contents xiii

The Kramer Two-Factor Ranked Analysis of Variance

(Two-Factor Repeated-Measures Design)
Worked Example: The Kramer Test
The Kruskal-Wallis One-Factor Ranked Analysis of Var
(One-Factor Completely Randomized Design)
Worked Example: The Kruskal-Wallis Test
The Page Test: Testing for a Ranked Tendency
Worked Example: The Page Test
The Jonckheere Test: Testing for a Ranked Tendency
Worked Example: The Jonckheere Test
Final Theoretical Note Regarding Nonparametric Tests

APPENDIX B BINOMIAL EXPANSION

B.l How to Calculate the Expansion: First Principles
B.2 How to Calculate the Expansion Without Effort

APPENDIX C PROOF THATSS, =SSB + SS, 379

C .1 Total Variance: Sum of Squares 3 80
C.2 Between-Treatments Estimate of the Total Variance:
Sum of Squares 380
C.3 Within-Treatments Estimate of the Total Variance:
Sum of Squares 381

APPENDIX D A NOTE ON CONFIDENCE INTERVALS 385

APPENDIX E SENSORY MULTIPLE-DIFFERENCE TESTING,

USING THE R-INDEX TO ASCERTAIN
DEGREES OF DIFFERENCE 389
E.l Degree of Difference Between Two Products Using Rating 390
E.2 Degrees of Difference Between Several Products Using
Rating 393

APPENDIX F DO WE HAVE THE WRONG TOOLS? 399

F.l Related Versus Independent Samples Designs and Storage
Studies 399
F.2 Rejecting Ho and Quality Assurance 399

APPENDIX G STATISTICAL TABLES 403

Key t o Statistical Tables
I I
TwoSanlple Difference Tests

One-Sample Tests Independent Samples Related Sarnplcs

Binomial test, Binomial conlparison McNemar test (fro1

page 5 7 of proportions, chi-squxe),
page 78 page 104
Chi-square
one-sample test, Chi-square
page 92 colnparison of dis-
tributions over two
Test a sample to independent
investigate dis- samples,
tribution of page 9 6
categories in
population
ann-Whitney U test, Sign test (from
page 309 binomial test),
page 7 3
Wilcoxon-Mann-
Whitney rank Wilcoxun test,
page 304

One-sample t lest, Independent- I

page 112 sarnplcs t test test,
page 117 page 115
Test whether sample
rnean is significant- Repeated-~neasures
ly dif'ferent randorni7cd ANOVA,
from population ANOVA, page 171
mean page 135
 More-Than-Two-Sample Difference Tests

I n d e p e n d e n t Samples Related Samples Two-or-More-

(Completely (Repeated I'actor
I Rdndomized) Measurcb) Difference Tests Correlation
Chi-square compari- Cochran Q test (from Contingency coeffi-
son of distributions chi-square). cient (from chi-
over more than page I05 square),
t w o independent I page 107

Kruskal-Wallis one- Friedman two-way TWO-FACTOR S p e a r n ~ a n ' srank

way ANOVA, ANOVA, TESTS O N L Y correlation, p ,
page 341 page 332 page 327
Friedman two-
Jonckhccre test: for Kramer test, way ANOVA.
ranked tendency, page 337 page 332
page 350
Kramer test,
Page test: for page 337
ranked tendency,
page 346 Papc test: for
ranked tendency
page 346

C o ~ n p l e t e l yran- Repeated-nlcasures Two-or-more- Pearson's product-

dornized ANOVA with factor ANOVA momcnt corrcla-
ANOVA with multiple com- with ~ n u l t i p l e tion, r ,
multiple com- parisons, comparisons, page 279
parisons, page 153, 171 page 153, 171,
page 135, 153 211
Sensory Evaluation of Food
 1
Before You Begin

1.1 HOW TO USE THIS BOOK

This book presents a simplified look at statistics, as do all introductory texts.
In the interests of clarity and simplicity some shortcuts will be taken, but
wherever possible, these will be indicated. Although the study of mathematical
statistics is clear enough, its application to real-life situations is not always so
clear. Statistical tests involve, in their derivation, certain assumptions about the
data and it is sometimes doubtful whether these assumptions are always fulfilled
in behavioral measurement. If they are not fulfilled, it is not always clear how
important this may be. For instance, in some experimental situations, should a
one- or a two-tailed test be employed? How much do data from intensity scale3
violate the assumptions for analysis of variance, and how important are such
violations? In what situations are nonparametric tests more desirable? These
matters will be discussed in more detail throughout the text; suffice it to say
that in the area of sensory and behavioral measurement such doubts should be
taken seriously. As far as possible, we will give recommendations and try to
indicate where there is disagreement. However, this text is not a statistical
treatise; it is not intended to give an exhaustive account of these controversies-
for that, go and get a degree or two in statistics! The aim of this text is to give
a working knowledge of statistical analysis with as few frills as possible. Enough
background will be given so that the reader can understand what a statistical test
is doing and when and how it should be used. The arguments and logic will
always be expressed as nonmathenlatically as possible.
1
2 Before You Begin

The mathematical formulas will be expressed simply; formal mathematical

notation with its welter of subscripts will not be used here. If it offends the
mathematical purist, I apologize, but a simpler, albeit more casual approach
makes teaching simpler at this level. So be warned that the notation used here
will not always be the standard notation used by statisticians. We will not bother
to put all algebra referring to populations in capital letters and that referring to
samples in lowercase letters. We will not bother to identify each value with its
own subscript; that only terrifies the reader and is bad teaching practice.
Furthermore, although most of the statistical tests will be illustrated by
examples from sensory measurement and the sensory analysis of food, this
principle will not be adhered to slavishly. Sometimes a statistical principle is
better illustrated by other examples, and in the interests of clarity and better
teaching, these will be adopted. For example, some probability theory is more
simply explained using familiar examp!es such as tossing of dice and coins.
Unfortunately, there is little agreement among the available texts on the best
approach to explaining the application of various statistical tests to behavioral
measurement; there is even disagreement in such texts over the names to be
given to various tests. The babel is best seen by a consideration of all the
different names given in behavioral texts to the various analysis-of-variance
procedures (Table G.10 in Appendix G). In this text, we will give statistical
tests the simplest yet most fully descriptive names possible; we will also keep
the use of jargon t o a minimum-we are not trying to impress anyone.
Finally, this text is brief, sparse, and not very conversational. It is hoped
that the starkness will eliminate distraction and allow the reader to stick strictly
to the point. It is hoped that this will speed the process of learning without
leaving the reader with a sense of desolation.

1.2 THE USE OF STATISTICS I N SENSORY EVALUATION

VERSUS ITS USE IN PSYCHOPHYSICS, EXPERIMENTAL
PSYCHOLOGY, AND CONSUMER TESTING
The experimental and statistical skills used for the sensory analysis of foods or
consumer testing are very similar to those used in experimental psychology.
For further study, texts on psychological statistics are recommended. There
are, however, some important differences between the application of statistics
t o sensory evaluation and its application to psychology or consumer testing.
These are due to the different goals and approaches of the various disciplines.
Often the differences are not important, but sometimes they are crucial because
they can alter the actual mathematical operations involved in statistical tests;
for example, analysis of variance. So, dear reader, if you are an experimental
psychologist or a consumer researcher about to use this text as a simple
reminder, or if you are a sensory analyst about to consult a psychology text,
please read this section beforehand to avoid confusion.
The Use of Statistics 3

First, let us look a t differences between sensory evaluation and experimental

psychology, of which sensory psychophysics is an important part. Generally,
in experimental psychology the goal is t o find out something about the behavior
of people in general, or even of animals. In sensory psychophysics, specifically,
the goal is t o examine the functioning of the senses and the brain, and the focus
is generally on people. In both these areas, the general strategy is t o take a
random sample from the population of people under consideration. The sample
is examined in a suitable experiment, the appropriate statistical analysis is
performed o n the sample, and using this analysis, conclusions are drawn about
the population. A sample is used because there is generally not enough time or
money t o examine the whole population. The sample is generally picked as
randomly as possible in the hope that it will match the population as closely as
possible.
In the sensory evaluation of foods or other products, there can be more than
one goal. First, in some situations, people may be used for a preliminary analysis
to measure the flavor of the food and provide clues for later chemical or physical
analysis. They may be used t o measure, say, flavor changes due t o a change in
the processing of the food. The question here is: Does the new processing
procedure alter the sensory characteristics of the food? Changes in sensory
characteristics can provide clues about changes in physical characteristics.
Should judges detect a change in the odor, it would provide a clue to chemists
to investigate changes in the volatiles. Should judges detect a change in sourness
it would indicate that chemists might first want to investigate changes in acid
content or even sugar content. The goal is to seek information about the food,
not the people who taste the food. A random sample of people is not selected.
A panel comprising of a few people is selected and trained to become judges in
the specific task required, whether it be detecting off-flavors in beverages or
rating the texture of cakes. Generally, potentiai judges are screened to see
whether they are suitable for the task a t hand. This careful selection of judges is
in no way a random sampling and is the first reason why the judge could not be
regarded as representative of the population in general. The training then given
t o the selected judges will usually make them more skilled in their task than the
general population would be; this is a second reason why they can no longer be
regarded as representative of the population from which they were drawn.
Our judges are not a sample to be examined so that conclusions can be drawn
about a population; they are the population! They are a population of labora-
tory instruments used t o study samples of food. On the other hand, the food
samples tested are samples from which conclusions can be drawn about the
populations of foods. They may be samples of a food processed in different
ways from which conclusions may be drawn about the whole population of that
food processed in these ways. So the focus of the study is very much on the
food; the judges are merely instruments used for measuring samples of food.
Logically, one good judge would be adequate for this purpose, as is one good
4 Before You Begin

gas chromatograph or atomic absorption spectrometer. More than one judge is

used as a precaution because unlike a gas chromatograph, a judge can become
distracted; the cautious use of second opinions can provide a useful fail-safe
mechanism.
Having detected a change in flavor under such circumstances, the question
becomes: Can this flavor change be detected by the consumers or people other
than trained panelists? It is possible that the flavor change is so slight as to be
detectable only by highly trained judges. One way of answering this question is
to take a sample of untrained people and test them to see whether they can
detect the flavor change. Then from this sample, inferences can be made about
the population of untrained people in general. The experiment now becomes
similar to one in sensory psychophysics. People are sampled, tested, and con-
clusions are then drawn about people in general. This is the second use of
sensory evaluation.
It can thus be seen that in the sensory evaluation of food, depending on the
aims of the study, conclusions may be drawn only for those judges actually
tested or they may be drawn for the whole population of people from which the
judges were sampled. It is reasonable to expect the probabilities and the statisti-
cal procedures to vary for these two cases. The difference becomes important
for analysis of variance, where different denominators are used for calculating
F values. Where the conclusions apply only to the judges tested, the judges are
said to be a fixed eflect. Where they apply to the whole population from which
the judges were drawn, the judges are said to be a random effect. In this text,
the analysis-of-variance procedures all use people as fixed effects because they
are easier t o understand and learn that way. To use people as random effects
when the aim is to study people per se, the calculation is altered very slightly.
The brief chapter (Chapter 13) on fixed- and random-effects models shows the
slight differences required when people are "random effects." Be sure to read it
before you perform an analysis of variance on data designed to give you facts
about people in general rather than only those people tested in the experiment.
It is worth noting that texts on psychological statistics differ from this text.
In psychological texts people are generally treated only as random effects.
The traditions of psychophysics and sensory evaluation vary even more.
In psychology or psychophysics, people are the subject of the investigation and
thus tend to be called subjects. In sensory evaluation, or sensory analysis as it
is also called, people are often specially selected and trained and tend to be
referred to as judges. In sensory analysis, judges are tested while isolated in
booths; experimenters and judges communicate in writing. In sensory psycho-
physics, the experimenter and subject often interact and communicate verbally;
this requires special training for experimenters so that they do not influence or
bias the subjects. The methods of sensory analysis are often derived from those
of psychophysics, but care must be taken when adapting psychophysical tests to
The Use of Statistics 5

sensory evaluation. This is because the goals of the two disciplines are different
and they can affect the appropriateness of various behavioral methods of
measurement to be used, as well as the statistical analysis employed. In psycho-
physics, the aim is t o measure the "natural" functioning of the senses and
cognitive processes. Extensive training will not be given if it alters a subject's
mode of responding or recalibrates the person, so obscuring his or her "natural"
functioning.
Consumer testing must also be considered. Whereas judges are used in sensory
analysis t o assess the flavor of a food per se, consumer testing selects samples of
consumers from the marketplace to find out whether they like or will buy the
food. For consumer testing, the people must be as representative as possible of
the potential buying population so that they do not give a misleading indication
of potential sales. To perform consumer tests on members of a panel used for
sensory analysis would be t o use a sample that was not representative of the
potential buying public. The panel would be comprised of a few people who
had been specially selected for their skill in sensory evaluation and who may
also have been made atypical as a result of their training. There is another
important point: The judges on a sensory panel are usually recruited from the
company; they are often laboratory or office workers who take time from their
regular duties to participate in the panel. These judges may have a knowledge of
the product which may bias their judgments of whether they like it; they may
even select responses that they feel might impress their bosses. Thus, t o use a
sensory panel for consumer testing would seem unwise. Despite this, some food
companies blindly adopt this research strategy.
The sample of consumers tested should be as representative as possible of the
potential buying population. It is a sample from which conclusions are drawn
about the population and so, as with sensory psychophysics, the people tested
are considered t o be random effects! Psychophysicists tend to pick a sample
as randomly as possible in the hope that it will turn out t o be representative
of the population. The strategy of random sampling is used in consumer testing,
but another strategy is also adopted. Here the demographics of the population
are studied and a sample carefully chosen so as to match the population demo-
graphically as closely as possible. If the population has 80% males, so will the
sample. If the population has 50% Hispanics, the sample will also. This approach
to making the sample as representative as possible of the population is called
stratified sampling. Sampling will be discussed further in the section on the
perils of sampling (Section 1.4).
As you read through the text, it will become apparent that statistical tests
are set up t o show that differences exist between sets of data. However, should
the reader be using statistics for quality assurance, trying to show that a
reformulated product has the same flavor as the regular product, the aim will
be to show that differences do not exist. This is using statistical analysis in a
6 Before You Begin

rather different manner, and readers are advised t o consult Appendix F before
analyzing their data.

1.3 WHY USE STATISTICS ANYWAY?

In Section 1.2 we mentioned the various disciplines that use statistics. We
discussed their sampling techniques and whether inferences made from samples
were carried to populations. T o summarize, psychophysicists, experimental
psychologists, and consumer testers are interested primarily in the behavior or
likes and dislikes of people. They make their measurements on a sample of
people, for reasons of time and economy, and use a statistical analysis t o make
inferences about the population from which the sample was taken. Naturally,
the larger the sample, the more representative it is of the population. The
more powerful the statistical test applied t o the sample, the better the infornia-
tion obtained about the population. This is true, of course, as long as all the
conditions required for the proper application of the test are fulfilled. There are
procedures for estimating how large a sample should be taken for a given test
of a given power, but these will not be dealt with here. Sensory analysts select
and train a small panel t o assess samples of foods. Conclusions resulting from
the statistical analysis may or may not be generalized further beyond the actual
panelists tested; the panelists may be random or fixed effects. In the same
way, conclusions may or may not be generalized beyond the actual food servings
tested. Replicate servings of a given food processed in a given way (often called
"replicates" or "reps") can themselves be seen as a fixed or a random effect,
depending on how far the conclusions are to be generalized.
Statistical tests may be inferential; inferences are made about populations
from samples. Statistical procedures may also be merely descriptive; the data
in the sample may merely be summarized in some way with n o inferences
being made about the population. Thus a mean is a summary of the data in a
given sample; it gives a central summary value of the data. In this way, it is
descriptive. However, the best estimate of the mean of a population is also the
mean of the sample. In this way, it is inferential.
It is important when taking a sample or designing an experiment to remember
that no matter how powerful the statistics used, the inferences made from a
sample are only as good as the data in that sample. Furthermore, the inferences
made from a sample apply only to the population from which the sample was
taken. This can be crucial in medical or consumer-orientated research.

Some Definitions and Jargon

Descriptive statistics are used t o describe the data obtained: graphs, tables,
averages, ranges, etc.
Some Perils of Sampling 7

Inferential statistics are used to infer, from the sample, facts about the popula-
tion.
A parameter is a fact concerning a population.
A statistic is a fact concerning a sample.
Data is plural; the singular is datum.

1.4 SOME PERILS OF SAMPLING

Generally, statistical tests require that a sample be selected randomly from the
population it is representing. This may be a complex matter but there are
additional points of design which are worth mentioning here.
When sampling, you must select your sample in an unbiased manner. If you
wish t o take a sample of university students and examine the sample to see
whether there are more men than women in the university, it is no use standing
outside the men's toilet. You will only get the occasional brave, or lost, lady
and so have a biased sample. This type of thing happened when in 1936 the
Literary Digest polled 10 million Digest readers and phone subscribers as t o
who would win the U.S. presidential election. The poll indicated Landon, but
in fact Roosevelt was elected. The Literary Digest had taken a biased sample.
Only the richer people read the Literary Digest or had phones at that time.
The poorer Americans tended not to be included in the poll; it was their vote
which elected Roosevelt.
S o take care how you collect your data. If the sample is chosen so as t o be
representative of the population, the conclusions drawn from the statistical
analysis apply only to that population. The sample may be selected by carefully
matching its demographics t o that of the population (stratified sampling), or a
representative sample may be obtained b y randomly sampling from the popula-
tion. I n practice, a so-called random sample is rarely completely random.
Subjects are usually volunteers and volunteers are not necessarily representative
of the population from which they were drawn. Also, an experimenter's method
of choosing subjects is hardly likely to be random; he or she will tend to select
those who are friendly and enthusiastic, as well as those who are conveniently
near the testing location. Because much psychological research is conducted in
psychology departments at universities, many of the subjects will be students.
Thus, many of the findings in psychophysics were established using samples of
willing, perhaps friendly, and enthusiastic students who work in or near
psychology departments; such samples are hardly representative of the popula-
tion at large. The importance of such difficulties is more a matter of philosophy
than mathematics; it is certainly hard t o gauge. Unfortunately, such questions
are rarely considered; experimenters seem t o hope that they are unimportant.
8 Before You Begin

Members of the sample may also tell lies. This can occur when questions of
a personal nature (money, sex, etc.) are asked. In fact, if a subject guesses the
correct answer or feels that a certain response is right, he or she may give it to
you, regardless of whether or not it is true for him or her. The tendency to
please the experimenter and give the "right" answer is strong among human
subjects.
Control groups are often used to control for any other effects that might
be happening. With drug research, some patients are put in a control group
and are not given the drug being tested. Instead, they are given a harmless
placebo, which resembles the drug but has no action. Often these control
patients are cured as well-the "placebo effect." By comparing those who took
the drug with those who thought they took the drug, a measure can be obtained
for the efficacy of the drug itself, independent of any suggestion effects. Control
groups are often used in behavioral research and could sometimes be useful in
the sensory analysis of foods.
I t is not the intention here to go into detail about sampling procedures;
there are plenty of texts that do this. Suffice it to say that one of the common
errors in sensory work with foods is to sample from the wrong population. If
you wish to sample the likes and dislikes of consumers, do not ask people who
are not representative of consumers, such as expert panelists or scientists
working on the food product itself.
A final point. No amount of sophisticated statistical analysis will make good
data out of bad data. There are many scientists who try to disguise badly con-
structed experiments by blinding their readers with a complex statistical
analysis. Be cautious about all scientific papers, especially in behavioral research
(sensory analysis, psychology, etc.); some of them contain grotesque nonsense.