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Autophagosomes engulf cytosol or organelles and deliver them to lysosomes to be degraded and released as nutrients. Mitochondrial outer membrane contributes to their biogenesis during starvation, according to a new study.
Autophagosomes engulf cytosol or organelles and deliver them to lysosomes to be degraded and released as nutrients. Mitochondrial outer membrane contributes to their biogenesis during starvation, according to a new study.
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Autophagosomes engulf cytosol or organelles and deliver them to lysosomes to be degraded and released as nutrients. Mitochondrial outer membrane contributes to their biogenesis during starvation, according to a new study.
Copyright:
Attribution Non-Commercial (BY-NC)
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Als PDF, TXT herunterladen oder online auf Scribd lesen
Nature Reviews Molecular Cell Biology | aoP, published online 9 june 2010; doi:10.1038/nrm2920
A u tO P H AGy Autophagosomal membranes
should contain lipid components of
From one membrane to another mitochondrial outer membranes if
they originate from them. The lipid mitochondrial outer phosphotidylserine is transferred from During starvation-induced autophagy, not due to mitophagy (the selective the endoplasmic reticulum (ER) to membrane double-membrane structures called autophagic capture of mitochondria), mitochondria, where it is converted to contributes to autophagosomes engulf cytosol or as inner mitochondrial membrane phosphotidylethanolamine, which is organelles and deliver them to lysosomes and mitochondrial matrix markers did conjugated to LC3 in autophagosome [autophago- to be degraded and released as not overlap with starvation-induced membranes. A fluorescently labelled some] nutrients. The origin of autophagosome CFP–LC3-positive structures. analogue of phosphotidylserine appeared biogenesis membranes was unclear, but Hailey et al. So, could the mitochondrial outer on starvation-induced autophagosomes, present evidence suggesting that membrane be used in autophagosome and perturbing ER–mitochondrion during the mitochondrial outer membrane formation during starvation? The contacts to block lipid transfer prevented starvation contributes to their biogenesis during authors visualized NRK cells expressing the formation of starvation-induced starvation. Mito-red fluorescent protein (which autophagosomes. This supports the To identify membranes that localizes to mitochondria) and idea that autophagosome membrane contribute to autophagosomes, the fluorescently tagged autophagosome lipids originate from mitochondria in authors expressed fluorescently protein markers (LC3 or autophagy starving cells. labelled proteins targeted to different protein 5 (ATG5)) under starvation In short, this study suggests that the organelles in a normal rat kidney and observed that regions of LC3 and mitochondrial outer membrane is at (NRK) cell line expressing cyan ATG5 staining first appear associated least one source of autophagosome fluorescent protein (CFP) fused to with mitochondria. Furthermore, membranes during starvation. the late autophagosome marker LC3 transmission electron microscopy Katharine H. Wrighton (also known as MAP1LC3B). Only a showed that autophagosomes appear mitochondrion-targeted isoform of adjacent to mitochondria. Finally, ORIGINAL RESEARCH PAPER Hailey, D. W. et al. cytochrome b5, which specifically photobleaching of fluorescently labelled Mitochondria supply membranes for autophagosome biogenesis during starvation. labels the mitochondrial outer mitochondrial membranes also depleted Cell 141, 656–667 (2010) membrane, extensively overlapped with signal from associated autophagosomes, fuRtHER REAdING Nakatogawa, H. et al. CFP–LC3-labelled autophagosomes. indicating that mitochondria and Dynamics and diversity in autophagy mechanisms: lessons from yeast. Nature Rev. Mol. This occurred specifically during autophagosomes share membrane, Cell Biol. 10, 458–467 (2009) starvation-induced autophagy and was at least transiently.