LAAN-A-LC-123

Application Data Sheet

High Performance Liquid Chromatography No. 52
High-Speed Analysis of Tetracyclines
Tetracyclines are one type of antibiotics and are widely used as drugs for animals.
Here we present a UFLC-UV separation of 3 components, oxytetracycline, tetracycline, and chlortetracycline.

mAU
4.0 ■Peaks

3.5 2 1. Oxytetracycline
2. Tetracycline
3.0 1 3. Chlortetracycline
2.5

2.0 3

1.5

1.0

0.5

0.0

-0.5

0.00 0.25 0.50 0.75 1.00 1.25 min

Analysis of Tetracyclines
[Sample Preparation]
A 1 g/mL concentration solution of each standard sample was prepared using a mixed solution of methanol
and 10mmol/L oxalic acid aqueous solution (3:2 v/v).

Analytical Conditions
Instrument : Prominence UFLC system
Column : Shim-pack XR-ODS (50 mm  3.0 mm i.d.)
Mobile Phase : A) 10mmol/L oxalic acid aq.
B) 10mmol/L oxalic acid aq. / acetonitrile (1/1 v/v)
B.conc; 18% to 60% (0 to 1.0 min), 18% (1.01 to 2.0 min)
Flow Rate : 1.2 mL/min
Column Temperature : 40 C
Detection : Absorbance at 360 nm（Ref. Correction 450nm）with semi-micro flow cell
Sample Volume : 5 µL

SHIMADZU CORPORATION. International Marketing Division

3, Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone. 81(3)3219-5641 Fax. 81(3)3219-5710
URL http://www.shimadzu.com Issued November, 2008