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Turbidimetry
The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.
NAME HB
UNITS g/dL
WAVELENGTH 550 nm
BIC. REF. 700 nm
SAMPLE VOLUME 45 µL
1ST REAGENT VOLUME 255 µL
2ND REAGENT VOLUME
REFERENCE Standard
NAME HB A1c
UNITS g/dL
WAVELENGTH 340 nm
BIC. REF.
SAMPLE VOLUME 12 µL
1ST REAGENT VOLUME 300 µL
2ND REAGENT VOLUME 60 µL
REFERENCE Standard
CALCULATION
The HbA1c percentage in the sample is calculated using the following general formula:
HbA1C (g/dL)
% HbA1C - IFCC = x 100
Hb (g/dL)
The values are traceable to IFCC Reference Method.
The traceable values to Reference Method as described by the US National Glycohemoglobin
Standardization Program (NGSP) are calculated using the following general formula:
A calculated test result is not actually performed on the system, but calculated from other test results
that have been run on the system.
All parameters related to calculated results must be programmed in the INFORMATION DATA <
TESTS < CALCULATED menu.
* Variable/Method: define the method assignation entering the HbA1c IFCC or NGSP
formulas described above. The selected variable must be assigned to an existing method stored in
the ‘Methods table’.
* Reference Range: use this area to define the normal Low & High ranges for men and
women. If the result for this test fall outside the ranges entered here, the system issues an alarm.
The existing Calculation Items are available for selection at Patient Entry screen. It is necessary to
request the tests used in the calculation to calculate a ‘Calculated Test’. Calculated results are only
printed if all data needed for the calculation are available.