Countender20 Mo Eng v12.00

Revision History
Version Date Modification

11.00 18/05/2010 First release
11.00 B 02/12/2011 Various updates
12.00 25/06/2012 Various updates
COUNTENDER 20+_Operator Manual_ENG_V12.00 | 3

Table of contents
1. Description .........................................................................................15
1.1. PRESENTATION ..............................................................................................................................15
1.1.1. HOST ............................................................................................................................................ 15
1.1.2. ACCESSORIES ............................................................................................................................... 15
1.1.3. REAGENTS .................................................................................................................................... 15
1.2. SPECIFICATIONS .............................................................................................................................15
1.3. FRONT VIEW ..................................................................................................................................19
1.4. REAR VIEW .....................................................................................................................................19
1.5. OPERATION ....................................................................................................................................20
1.5.1. DISPLAY SCREEN .......................................................................................................................... 20
1.5.2. MENUS......................................................................................................................................... 20
1.6. DETECTION PRINCIPLE ...................................................................................................................23
1.6.1. DETECTION PRINCIPLES OF WBC, RBC, PLT ................................................................................. 23
1.6.2. MEASUREMENT PRINCIPLE OF HGB ............................................................................................ 24
1.6.3. RED BLOOD CELL VOLUME .......................................................................................................... 24
1.6.4. METHODS FOR PARAMETERS MEASUREMENT ........................................................................... 24
2. Installation ..........................................................................................27
2.1. PACK ..............................................................................................................................................27
2.2. PRE-INSTALLATION CHECKS...........................................................................................................27
2.3. INSTALLATION REQUIREMENTS ....................................................................................................27
2.3.1. AMBIENT REQUIREMENTS ........................................................................................................... 27
2.3.2. SPACE REQUIREMENTS ................................................................................................................ 27
2.3.3. POWER REQUIREMENTS .............................................................................................................. 27
2.4. REAGENTS CONNECTIONS .............................................................................................................27
2.4.1. LYSE CONNECTION....................................................................................................................... 27
2.4.2. DILUENT CONNECTION ................................................................................................................ 28
2.4.3. RINSE CONNECTOR ...................................................................................................................... 28
2.4.4. WASTE CONNECTOR .................................................................................................................... 28
2.5. RECORDER PAPER INSTALLATION..................................................................................................28
2.6. KEYBOARD AND MOUSE INSTALLATION .......................................................................................28
2.7. PRINTER INSTALLATION (OPTIONAL).............................................................................................28
2.8. BARCODE SCANNER INSTALLATION (OPTIONAL) ..........................................................................29
2.9. POWER CABLE CONNECTION ........................................................................................................29
3. Sample analysis .................................................................................31

3.1. BEFORE ANALYSIS ..........................................................................................................................31
3.2. START UP OF THE INSTRUMENT ....................................................................................................31
3.3. BACKGROUND TEST .......................................................................................................................31
3.4. QUALITY CONTROL ........................................................................................................................32
3.5. BLOOD SAMPLE COLLECTION ........................................................................................................32
3.6. COUNTING AND ANALYSIS ............................................................................................................33
3.6.1. INPUT SAMPLE INFORMATION .................................................................................................... 33
3.6.2. COUNT AND ANALYZE ................................................................................................................. 34
3.6.3. FLAGS ........................................................................................................................................... 35
3.6.4. HISTOGRAM FLAG ....................................................................................................................... 35
3.7. HISTOGRAM MODIFICATION .........................................................................................................36
3.8. TURN OFF.......................................................................................................................................36

4. Quality Control ...................................................................................39
4.1. QUALITY CONTROL EDIT ................................................................................................................39
4.2. QUALITY CONTROL RUN ................................................................................................................39
4.3. LEVEY-JENNINGS GRAPHS .............................................................................................................40
4.4. QUALITY CONTROL TABLE .............................................................................................................41
5. Calibration ..........................................................................................43
5.1. MANUAL CALIBRATION .................................................................................................................43
5.1.1. BACKGROUND TEST ..................................................................................................................... 43
5.1.2. CALCULATION OF THE NEW CALIBRATION FACTOR .................................................................... 43
5.1.3. MODIFY THE CALIBRATION COEFFICIENT .................................................................................... 43
5.2. AUTO CALIBRATION .......................................................................................................................44
6. Setup ...................................................................................................45
6.1. TIME ...............................................................................................................................................45
6.2. PRINTOUT ......................................................................................................................................46
6.3. CONFIGURATION ...........................................................................................................................47
6.4. REFERENCE RANGES ......................................................................................................................49
6.5. OPERATOR & DEPARTMENT INFORMATION .................................................................................49
6.5.1. ADD DEPARTMENT ...................................................................................................................... 50
6.5.2. MODIFY DEPARTMENT INFORMATION ....................................................................................... 50
6.5.3. DELETE DEPARTMENT INFORMATION......................................................................................... 51
6.5.4. PRINT DEPARTMENT INFORMATION ........................................................................................... 51
6.5.5. ADD DOCTOR INFORMATION ...................................................................................................... 51
6.5.6. MODIFY DOCTOR INFORMATION ................................................................................................ 52
6.5.7. DELETE DOCTOR INFORMATION ................................................................................................. 52
6.5.8. PRINT DOCTOR INFORMATION ................................................................................................... 52
7. Review ................................................................................................53
7.1. DATALOG .......................................................................................................................................53
7.1.1. REVIEW THE SAMPLE DATA ......................................................................................................... 53
7.1.2. DELETE DATA OPERATE ............................................................................................................... 53
7.1.3. DELETE ALL DATA OPERATE ......................................................................................................... 54
7.1.4. PRINT AS LIST OPERATE AS FOLLOW STEPS ................................................................................. 54
7.1.5. INQUIRE PRINT THE SELECT DATA AS FOLLOW STEPS................................................................. 54
7.2. DETAIL REVIEW ..............................................................................................................................54
8. Servicing .............................................................................................57
8.1. FLUSH.............................................................................................................................................57
8.2. ENHANCED FLUSH .........................................................................................................................58
8.3. CLEANING ......................................................................................................................................58
8.4. ENHANCED CLEANING ...................................................................................................................59
8.5. DRAIN CHAMBERS .........................................................................................................................59
8.6. PARK ..............................................................................................................................................60
8.7. PRIME DILUENT .............................................................................................................................60
8.8. PRIME LYSE ....................................................................................................................................61
8.9. PRIME RINSE ..................................................................................................................................61
8.10. WEEKLY MAINTENANCE ................................................................................................................62
8.11. MECHANIC .....................................................................................................................................62
8.12. SPECIAL ..........................................................................................................................................63
6 | COUNTENDER 20+_Operator Manual_ENG_V12.00

9. Maintenance......................................................................................65
9.1. ROUTINE MAINTENANCE...............................................................................................................65
9.2. DAILY MAINTENANCE ....................................................................................................................65
9.3. WEEKLY MAINTENANCE ................................................................................................................65
9.4. MONTHLY MAINTENANCE .............................................................................................................65
9.5. YEARLY MAINTENANCE .................................................................................................................66
9.6. MAINTENANCE ..............................................................................................................................66
10. Troubleshooting..................................................................................67
11. Help .....................................................................................................69
Appendices – TSB........................................................................................71
APPENDIX 1: SAFETY SYMBOLS...............................................................................................................71
APPENDIX 2: REAGENTS AND CONSUMABLES LIST ................................................................................73

Statement
SFRI sarl owns all rights to this unpublished work and intends to maintain this work as confidential.
SFRI sarl may also seek to maintain this work as an unpublished copyright. This publication is to be
used solely for the purposes of reference, operation, maintenance, or repair of SFRI sarl equipment.
No part of this can be disseminated for other purposes.
In the event of inadvertent or deliberate publication, SFRI sarl intends to enforce its rights to this
work under copyright laws as a published work. Those having access to this work may not copy, use,
or disclose the information in this work unless expressly authorized by SFRI sarl to do so.
All information contained in this publication is believed to be correct. SFRI sarl shall not be liable for
errors contained herein nor for incidental or consequential damages in connection with the
furnishing, performance, or use of this material. This publication may refer to information and
protected by copyrights or patents and does not convey any license under the patent rights of SFRI
sarl, nor the rights of others. SFRI sarl does not assume any liability arising out of any infringements
of patents or other rights of third parties.
Content of this manual is subject to changes without prior notice.
Responsibility on the Manufacturer Party

SFRI sarl is responsible for safety, reliability and performance of this equipment only in the condition
that:
 All installation, expansion, change, modification and repair of this equipment are conducted
by SFRI sarl qualified personnel;
 Applied electrical appliance is in compliance with relevant National Standards;
 The analyzer is operated under strict observance of this manual.
Warning
For continued safe use of this equipment, it is necessary that the listed instructions are followed.
However, instructions listed in this manual in no way supersede established medical practices
concerning patient care.
 This equipment is intended for use only by medical professionals in health care
institutions.
 To avoid electrical shock, you should not open any cover by yourself. Service must be
carried out by qualified personnel only.
 It is dangerous to expose electrical contact or applicant coupler to normal saline, other
liquid or conductive adhesive. Electrical contact and coupler such as cable connector,
power supply must be kept clean and dry. Once being polluted by liquid, they must be
thoroughly dried. If to further remove the pollution, please contact your biomedical
department or SFRI sarl.
It is important for the hospital or organization that employs this equipment to carry out a reasonable
maintenance schedule. Neglect of this may result in machine breakdown or injury of human health.
Upon request, SFRI sarl may provide, with compensation, necessary circuit diagrams, calibration
illustration list and other information to help qualified technician to maintain and repair some parts,
which SFRI sarl may define as user serviceable.

Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR
IMPLIED, INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR
PURPOSE.
Workmanship and Materials

SFRI sarl guarantees new equipment other than accessories to be free from defects in workmanship
and materials for a period of one year from date of shipment under normal use and service. SFRI
sarl's obligation under this warranty is limited to repairing, at SFRI sarl’s option, any part which upon
SFRI sarl's examination proves defective.
Exemptions
SFRI sarl's obligation or liability under this warranty does not include any transportation or other
charges or liability for direct, indirect or consequential damages or delay resulting from the improper
use or application of the product or the substitution upon it of parts or accessories not approved by
SFRI sarl or repaired by anyone other than a SFRI sarl authorized representative.
This warranty shall not extend to any instrument which has been subjected to misuse, negligence or
accident; any instrument from which SFRI sarl's original serial number tag or product identification
markings have been altered or removed, or any product of any other manufacturer.
Safety, Reliability and Performance

SFRI sarl is not responsible for the effects on safety, reliability and performance of the COUNTENDER
20+ if:
 Assembly operations, extensions, re-adjusts, modifications or repairs are carried out by
persons other than those authorized by SFRI sarl.
 Personal unauthorized by SFRI sarl repairs or modifies the instrument.
Warning
The COUNTENDER 20+ is an In Vitro Diagnostic instrument. It may indirectly bring risks to patients
and present a direct risk to operators and the environment during manipulation. To avoid these risks,
be sure to read the following instructions carefully.
This device uses AC 220V alternating courant. Do not open the rear cover before
switching off the power supply. If you need to open the rear cover of the device for
maintenance reasons, please contact our service personnel or professionals.
Patient samples are potentially contaminating agents. Manipulate with care using
appropriate protection equipment (glasses, gloves and laboratory coat) and be sure to
dispose of the waste in accordance with relevant regulations of your hospital.
Do not operate the device unless you have read this Operation Manual carefully

Company Contacts
Address: SFRI sarl
Lieu-dit Berganton
33127 SAINT JEAN D’ILLAC
FRANCE
Phone: +33 (0) 556 688 050
Fax: +33 (0) 556 217 903
E-mail : support@sfri.com

Foreword
The COUNTENDER 20+ is a Hematology blood cell Counter with 3 part leukocytes sub population
differentiation. The analyzer determines the following hematological parameters for whole-blood
specimens:
ITEMS ABBREVIATION UNITS

White blood cell WBC 103/uL
Lymphocyte LYM# 103/uL
Monocyte MON# 103/uL
Granulocyte GRAN# 103/uL
Lymphocyte Percent LYM% %
Monocyte Percent MON% %
Granulocyte percent GRAN% %
Red blood cell RBC 106/µL
Hemoglobin concentration HGB g/dL
Hematocrit HCT %
Mean cell volume MCV fL
Mean cell hemoglobin MCH pg
Mean cell hemoglobin concentration MCHC g/dL
Red Blood Cell Distribution Width
RDW-SD fL
Standard Deviation
Red Blood Cell Distribution Width
RDW-CV %
Coefficient of Variation
Platelet PLT 103/uL
Mean Platelet Volume MPV fL
Platelet Distribution Width PDW %
Plateletcrit PCT %
Platelet-large Cell Ratio P-LCR %
White BLood Cell Histogram WBC Histogram
Red Blood Cell Histogram RBC Histogram
Platelet Histogram PLT Histogram

1. Description
1.1. Presentation
1.1.1. HOST
Controls the process of sample gathering, dilution and analysis and includes the following units:
 Power Supply.
 Central control circuit.
 Dilution unit
 Volume measurement unit.
 Display unit.
 Thermal recorder.
1.1.2. ACCESSORIES
 PS/2 mouse.
 PS/2 keyboard.
 Printer
 Bar code scanner (Optional)
1.1.3. REAGENTS
Type Name Code

Diluent SFRI Diluent 3.1 code HSL321
Lyse SFRI Lyse 3.1 code HSL302
Detergent SFRI Cleaner 3.1 code HSG305
Conc Cleaner SFRI CleanEZ 3.1 code HSC302
Strong Cleaner SFRI Clair 3.1 code HSC102
Type Name Code

BloodTrol 16
3 x 3 ml Normal code R021005
Blood Quality Controls 3 x 3 ml LNH code R021001
6 x 3 ml Normal code R021002
6 x 3 ml LNH code R021006
Calibrator 1 x 3 ml BloodCal code R021003
Warning
Usage of non authorized reagents may incur inaccurate results or damage the instrument.
Only regular usage of Blood Quality Controls will certify the accuracy of sample results.
1.2. Specifications
Class
According to anti-electric shock: Grade II, Pollution 2
According to defend deleterious liquid: General Equipment (Closed Equipment)
According to disinfection method: Recommend by manufactory
According to safety degree: Do not use the equipment under the environment of mixed with
flammable air or the air is mixed with O2 and NO

According to working state: Continuous running Equipment
Detection principles
RBC, WBC, Plt : Electrical resistance detection impedancemetry
Hemoglobin: Cyanide free spectrophotometry
Parameters
20 parameters and 3 histograms
Measured parameters
ITEMS UNITS
WBC 109 / L
RBC 1012 / L
MCV fL
HGB G / dL
PLT 109 / L
MPV fL
Parameters given by histogram

ITEMS UNITS
LYM% %
MON% %
GRAN% %
RDW-SD fL
RDW-CV %
PDW %
P-LCR %
Calculated parameters
ITEMS UNITS
LYM# 109 / L
MON# 109 / L
GRAN# 109 / L
HCT %
MCH pg
MCHC g/dL
PCT %
Sample Volume
Venous & Capillary modes : 9.6µl
Prediluted mode: 20.0µL
Volume per test

Diluent : 20.2 mL
Rinse : 5.3 mL

Lyse : 0.50 mL
Dilution Ratio
Venous and Capillary mode:
WBC/HGB 1:300
RBC/PLT 1:44600
Prediluted Mode:
WBC/HGB 1:355
RBC/PLT 1:44500
Dimension of apertures
WBC aperture 100μm
RBC/PLT gem aperture 80μm
Display
8” LCD screen
High resolution: 640x480 mm
Color display
Language
English
Input/Output
Electrical input x 1
Print socket x 1
VGA x 1
RS-232 x 2
USB x 2
PS/2 x2
Printout
Fast thermal printer, 57.5 mm wide paper, recording width 48 mm
Optional external printer
Linearity and precision

PARAMETERS LINEARITY RANGE PARAMETERS LINEARITY RANGE
9
WBC 0.0 -99.9x10 /L GRAN# 0 -99.9x109/L
RBC 0.00 -9.99x1012/L HCT 0.0 -100.0%
HGB 00.0 -300g/L MCH 0.0 -999.9pg
9
PLT 0 -3000x10 /L MCHC 0.0 -999.9g/L
MCV 0 -250fL RDW-SD 0.0 -99.9 fL
LYM% 0 -100% RDW-CV 0.0 -99.9%
MON% 0 -100% PDW 0.0 -30.0%
GRAN% 0 -100% MPV 0.0 -30.0fL
9
LYM# 0 -99.9x10 /L PCT 0.0 -9.99%
MON# 0 -99.9x109/L P-LCR 0.0-99.9%

PARAMETERS PRECISION CV%
WBC ≤2.5%
RBC ≤2.0%
HGB ≤2.0%
MCV ≤1.0%
PLT ≤5.0%
PARAMETERS LINEARITY
WBC 0.0-99.9x109/L
RBC 0.00-9.99x1012/L
HGB 0-300g/L
MCV 40 -150fL
PLT 0-999x109/L
Operating Environment
Temperature: 18°C – 35°C
Humidity: < 70% (max humidity)
Atmospheric pressure: 86.0 – 106 kPa
Power Requirements
A.C. 110/220 V ±10%; 50 – 60 Hz
Fuse: A.C. 250 V; 2 A
Dimensions
330(W) x 430(H) x 380(D) mm
Weight
17,5 kg
Regulation
IVD 98/79

1.3. Front view
LCD Display
Sample probe
Thermal printer Start Key
1.4. Rear view

1 Equipotential ground terminal 2 PS/2 socket
3 PS/2 Mouse socket 4 Printer socket
5 USB socket 6 Reserved socket
7 RS232 socket 1 8 RS232 socket 2
9 Air filter 10 Waste sensor connector
11 Waste connector 12 Rinse connector
13 Diluent connector 14 Lyse connector
15 Power switch 16 Power source socket
17 Fuse holder
1.5. Operation
1.5.1. DISPLAY SCREEN
The display screen is divided into the following areas:
Menu section
Data display section Data display section
WBC graph section
PLT graph section RBC graph section Display section
Auxiliary operation section System time section

Figure 1-2
1.5.2. MENUS
You can operate the instrument by menu. Move the mouse to the required menu bar, and then press
the left key of the mouse, the required function will be selected. Pressing the left key out of the
menu area, the operation will be canceled.

MAIN MENU BAR:
Settings Pat. Info Service QC/Calib Datalog Print Mute Shutdown Help
A. SETTINGS:
Figure 1-4
Sample Modes:
Choose sample mode among the following:
 Venous Blood Mode,
 Capillary Blood Mode,
 Prediluted Mode.
Histograms:
Opens the histogram adjustment window of the current sample. You can manually adjust the
histograms of the current sample.
Setting:
Opens the function menu and allows you to chose between the following:
 Time: opens the time setting window to set the time of the instrument.
 Printout: opens the printout window to set the functions such as print or report.
 Config: opens the configuration window to set the functions such as print, sleep, clean, and
display etc.
 Para/Unit: allows you to select the units
 Limits: opens the limits window to modify the limits.
 Operator: opens the operator window to set the department, abbreviation, department and
operator.
About:
Display software and copyright information.
B. PAT. INFO:
Opens a single window allowing operators to set patient information.
C. SERVICE:
Flush: To get rid of the aperture clog.
Enhanced Flush: To get rid of hard aperture clog.

Cleaning: Run the normal cleaning program to clean the measurement unit.
Enhanced Cleaning: Clean the measurement unit with cleaner.
Drain Chambers: Empty the liquid of the counter pool.
Park: Run the Park Program to clean and empty the tubing.
Prime Diluent: Inject the Diluent to the appointed tubing.
Prime Lyse: Inject the Lyse to the appointed tubing.
Prime Cleaner: Inject the cleaner to the appointed tubing.
Maintenance: Run the maintenance program to maintain the instrument.
Mechanic: Enter the Mechanic window to verify the functions of the instrument.
Special: Enter the Special window to maintain the instrument.
Figure 1-5
D. QC /CALIB:
Figure 1-6

Quality Control
Allows you to choose between the following:
 QC Edit: Enter the QC edit window to edit the QC parameters.
 QC Run: Enter the QC run window to carry out the selected QC files.
 QC Graphs: Enter the QC graphs window to browse the selected QC graphs.
 QC Table: Enter the QC table window to browse the selected QC tables.
Calibration
Allows you to choose between the following:
 Manual Calibration: Enter the manual calibration window to calibrate the instrument
manually.
 Auto Calibration: Enter the auto calibration window to adjust the instrument automatically.
E. Datalog:
Accesses the datalog menu.
F. Print:
Allows you to print the displayed results.
G. Mute:
Stops the sound alert.
H. Shutdown:
Start the shutdown program to clean the measurement unit automatically. Shut off the power supply
when the screen displays “Turn off the power now”.
I. Help:
Accesses the system help window.
1.6. Detection principle

1.6.1. DETECTION PRINCIPLES OF WBC, RBC, PLT
The instrument counts and sizes the cells by detecting and measuring changes in electrical resistance
when a particle (such as a cell) passes through a gem aperture sensor.
1. Sample is diluted in a conductive liquid. Blood cells are bad conductors, each time a blood
cell will pass through the aperture a resistant signal will be generate.
2. When cell goes through the aperture, the resistance is increased as cell volume increases. As
shown on Figure1-4. According to the Ohm formulary: U=RI (U =Voltage I =Current R
=Resistance). If I is invariable, U is increased as cell volume increases.
3. Treat by magnifying circuit, the voltage signal is amplify, background noise is removed, and
receives the signal to analysis. As shown on Figure 1-4.
4. WBC and RBC/PLT are analyzed by two different circuits. The MPU analyzes and calculates
the cells, then gives the histograms.
5. The count of PLT adopts an advanced liquid, electron and soft system, which can settle the

repetitive count of the cells. If RBC enters the analysis area, they will have similar pulses with
PLT; it may cause confusion as repetitive count.
Figure 1-4
1.6.2. MEASUREMENT PRINCIPLE OF HGB

Adding lyse into the blood sample will crack the membrane of red blood cells promptly and transfer
into a kind of compound which can absorb the wavelength of 540 nm. Absorbance background is
performed with diluents. Measurement of the Optocal density of the sample allows calculation of the
hemoglobin concentration.
1.6.3. RED BLOOD CELL VOLUME

Amplitude of the pulses is correlated to the sizes of the RBC, the instrument measures each cell and
classify it according to its volume by a pre-set classification program. All the cells fall into categories
(WBC, RBC, PLT etc.) as follows:
WBC 120—1000 fL
RBC 82—98 fL
PLT 2—35 fL
According to the volume, leucocytes handled by lyse can be subdivided into three categories:
lymphocyte(LYM) Monocyte (MON), granulocyte (GRAN) as follows :
LYM 35—90 fL
MON 90—160 fL
GRAN 160—450 fL
1.6.4. METHODS FOR PARAMETERS MEASUREMENT

The parameters of blood test are expressed in 3 ways:
Measured WBC, RBC, PLT, HGB, MCV, MPV.

Histogram LYM%,MON%,GRAN%, ,RDW-SD,RDW-CV, PDW, P-LCR
Calculated LYM#, MON#, GRAN#, HCT, MCH, MCHC, PCT

 WBC, RBC, PLT are counted
 HGB is measured
 MCV is determined by measuring the average volume of individual erythrocytes. This number
is multiplied by a coincidence correction factor and a calibration factor. The reported value
expresses MCV in femtoliters.
 RDW represents the size distribution spread of the erythrocyte population derived from the
RBC histogram. It expresses the coefficient of variation as RDW-CV, the unit is %, and express
the standard difference as RDW-SD, the units is fL.
 MPV is the average volume of individual platelets derived from the PLT histogram. It
represents the mean volume of the PLT population and expressed in femtoliters (fL).
 PDW is derived from the PLT histogram; it represents the geometry standard difference of
the volume of the PLT population.
 P-LCR is derived from the PLT histogram, it represents the ratio of the bigger PLT and
expressed in %.
 HCT(%)= RBC x MCV/10
 MCH(pg)= 10 x HGB/RBC
 MCHC(g/L)= 100 x HGB/HCT
 PCT(%)= PLT x MPV/10
 LYM% = 100 x AL /(AL+AM+AG)
 MON% = 100 x AM /(AL+AM+AG)
 GRAN% = 100 x AG/(AL+AM+AG)
 LYM# = LYM% x WBC/100
 MON# = MON% x WBC/100
 GRAN# = GRAN% x WBC/100
AL: Number of cell in area of LYM;

AM: Number of cell between area of lymphocyte and area of granulocyte;
AG: Number of cell in area of GRAN;

2. Installation
2.1. Pack
When you receive your COUNTENDER 20+, carefully inspect the carton box. If you see signs of
mishandling or damage, file a claim with the carrier immediately.
2.2. Pre-installation checks

1. Take out instrument and accessories from the carton box
2. Keep the packing material for future transport
3. Check accessories according to the enclosed packing list.
4. Check the status of instrument and accessories.
2.3. Installation requirements

2.3.1. AMBIENT REQUIREMENTS
Put instrument on flat and clean platform. Avoid direct sunshine and dust.
Caution:
 Keep avoid of high-power equipment such as Centrifuge, CT, MRI, X-ray.
 Do not use emission radiation equipment nearby, it may cause interferences.
2.3.2. SPACE REQUIREMENTS

At least 20cm on each side is the preferred access to perform service procedures.
Note: put reagents at the same height.
2.3.3. POWER REQUIREMENTS

 220(1±10%) VAC
 50 HZ
 150VA
An independent grounded power socket
A grounded outlet is required to connect with the grounding pole on the rear panel. Be sure to
guarantee the security of the work site.
Caution: power will seriously decrease the performance and reliability of the instrument. Proper
action such as the installation of UPS (not provided by manufacturer) should be taken before
operation.
2.4. Reagents connections

There are four tube connectors in the back panel with color of black, green, blue and red. Each of
them is wrapped with a cap to avoid contamination. Uncover and store the caps carefully on first
installation.
2.4.1. LYSE CONNECTION

Take out the lyse tube with red connector; plug it to the red connector in the rear panel.
Put the other side of the tube in the SFRI Lyse 3.1 bottle, and then screw down the cover.
2.4.2. DILUENT CONNECTION

Take out the diluent tube with green connector, plug it to the green connector in the rear panel.
Put the other side of the tube in the SFRI Diluent 3.1 bottle, and then screw down the cover.
2.4.3. RINSE CONNECTOR

Take out the rinse tube with blue connector, plug it to the blue connector in the rear panel.
Put the other side of the tube in the SFRI Cleaner 3.1 bottle, and then screw down the cover.
2.4.4. WASTE CONNECTOR

Take out the WASTE tube with black connector, plug it to the black connector in the rear panel.
Connect BNC plugs with BNC socket marked “WASTE” in the back panel.
Screw down the WASTE collection container cover.
Caution:
 Ensure all the tube in a natural condition, without distortion.
 Install the tubes with hand, do not use tools.
 Use the suitable reagent with instrument. Otherwise it may cause inaccurate result or
damage the instrument.
 Keep reagents away from direct sunshine.
 Discard the residual reagent when change reagent pact, do not pull it into new reagent pact,
it may cause pollution.
 Keeping the tube away from other things, avoid of pollution.
 Do not use the frozen reagent.
 Do not use the expired reagent.
Warning
The waste must be handled with biochemical or chemical methods before outlet to the drainage,
or it will cause contamination to the environment. Hospital and laboratory have obligation to
follow the environmental regulation.
2.5. Recorder paper installation

1. Gently press the recorder door and open it.
2. Insert the new paper against the paper mouth; let the print side towards the thermal head.
3. Pull the paper in the other side of the printer, keep it straight.
4. Pull the paper through the paper exit.
2.6. Keyboard and mouse installation

1. Remove keyboard, mouse from the shipping container.
2. Plug keyboard cable into a socket marked in rear panel.
3. Plug mouse cable into a socket marked in rear panel.
4. Keyboard and mouse can be positionned at your convenience.
2.7. Printer installation (Optional)

Remove the printer from the shipping box along with the manual. Install the printer according to the
printer’s manual.
Note: plug printer cable into a socket marked in rear panel.
2.8. Barcode scanner installation (Optional)

It is possible to connect a bar code reader on PS/2 keyboard plug.
The system is then instantly ready for work
2.9. Power cable connection

The switch which guarantees the instrument back plate is in the state of closing (0), inserts the whole
end of the cable plug in the instrument outlet, insert another end of the cable in a power socket.
Connect the earth post of the back plate of host computer with earth connection directly.
Ensure the power is suitable for the requirement

3. Sample analysis
3.1. Before analysis

Check the instrument as follows:
1. Check the tubing is in good place without distortion.
2. Check the cable and socket.
3. Check the connection of the accessories.
3.2. Start up of the instrument

Switch on the external printer if connected.
Turn on the instrument on the rear panel of the instrument, the indicator lamp in the front panel
begins to glimmer, the instrument begins initializing.
First, the instrument will check if reagents are available and clean the liquids tubing. Since examining
and finishing, the instrument has entered the blood cell analyzing window. As shown on Figure 3-1. If
there is malfunction, the information section will display the corresponding message.
Figure 3-1
3.3. Background test

First perform a background test in order to control the cleanliness of the instrument
The acceptable range of results for the background is:
PARAMETER BACKGROUND RANGE UNIT

WBC ≤0.3 109/L
RBC ≤0.03 1012/L
HGB ≤0.2 g/L
HCT ≤0.5 %
PLT ≤10 109/L

If this background test data exceeds this range, repeat the above test step, until it can be accepted. If
carry on five times, the data didn’t reach the requirement still, please refer to chapter 10 "
troubleshooting" of this manual.
Note: there is a specific sample ID number to perform the background which is 999999999. Then
the analyzer will only display results for WBC, RBC, HGB, MCV, PLT. These background tests are not
saved in the datalog.
3.4. Quality control

Only regular usage of Blood Quality Controls certify the accuracy of sample results
By using our quality controls and the Quality Control menus included in the software of your
COUNTENDER 20+, you’ll be able to survey the quality of the results
Qualtity control tests may be run at the first installation or every day before testing the blood
samples.
Please refer to chapter 4 “Quality Control”.
3.5. Blood sample collection
Warning
Be very careful when manipulating samples, controls and calibrators Wear suitable protections
equipments.
Use EDTA-K3 or K2, anticoagulant tubes.
3.5.1. SAMPLE FOR VENOUS MODE

Common mode for classic blood collection through 12*75mm tubes
3.5.2. SAMPLE FOR PRE-DILUTED MODE

1. Adopt 20 µL capillary into 20µL blood cup, wipe the outer of the cup, then fill the blood in to
sample cup.
2. From the blood cell analyzer windows, click SETTINGS > SAMPLE MODES > PRE-DILUTED
MODE. Then select PRIME DILUTION, it will spring a add dilution window.
3. Put a clear sample cup under the probe, press START key, the instrument will dispense 1.5ml
of diluent. The indication LIGHT glitters fleetingly. When the indicator glitters very slowly,
scrape the dripping into the cup.
4. When dilution is finished get out of this menu to aspirate the dilution.
Caution: Put the sample cup under the probe acclivitous, the liquid will flow along the cup wall
and this will avoid bubbles.

Figure 3-2
3.5.3. SAMPLE FOR THE CAPILLARY MODE

Depuis le menu principal sélectionnez SETTINGS > SAMPLE MODES > CAPILLARY.
Il n’y a aucune différence technique entre les analyses réalisées dans les modes venous et capillary.
L’unique différence se situe dans l’historique des analyses. Ou l’on sera capable de trier les tests en
fonctions du mode d’analyse sélectionné.
1. Aspirate the sample from the capillary equipment.

2. There is no difference in the counting principle between venous and capillary mode.
3.6. Counting and analysis

For counting and analysis follow the below steps.
3.6.1. INPUT SAMPLE INFORMATION

3.6.1.1. MANUAL INPUT
In the blood cell analyzing window, click INFO. A data editor window will open. Click the input text
frame or checkbox with mouse and ill-in as desired:
Name: up to24 digits.

First name: up to24 digits.
Pat. ID: up to24 digits.
Sex: choose ladies or gentlemen. If nothing has been chosen, the default is blank.
Age: choose year, month and day. While choosing year you can input 3 Figures at most. While
choosing the month and day you can input 2 Figures at most.
Sample: the currently sample type.
ID: up to 9 digits.
Send by: input 12 letters at most or choose the recorded information.
Operator: input 12 letters at most or choose the recorded information.
Limits: choose between current, men, woman, child, neonate, a self-defining 1, self-defining 2, self-
defining 3 and automatic. While choosing automatic, the instrument automatically provides the
reference value (table3-2).

REFERENCE VALUE AGE SEX
current Not input Blank ,Man, Woman
current >13years old Blank
current >13years old Man
woman >13years old woman
child >1month and<=13years Blank ,Man, Woman
neonate <1month Blank ,Man, Woman
Table 3-2
Figure 3.3
Click OK, the instrument keeps the data and returns to the analyzing window.
Click CANCEL, the instrument cancels the data and returns to the analyzing window.
Note: No.999999999 is the special number of background test.
Input the data with the bar code scanner

Scan the bar code, it will read the sample data automatically.
Note: If there is a batch of samples, input the sample information in the Review window index
with serial number when the batch test is over.
3.6.2. COUNT AND ANALYZE

1. Put the sample cup under the probe, press the start pad, the indicator lamp of the panel
began to glimmer, shift out the sample cup after the indicator lamp stops glimmering.
Note: Be careful to mix correctly the tube before aspiration
2. The instrument begins to analyze sample automatically, please wait for the analysis result.
3. After this processing, the result will display on the window with the histogram of WBC, RBC,
PLT.
4. If there is clog or bubbles during the process, the information section would display the flag
of “Clog failure” or “Bubble”.
Note: Sample probe is automatically cleaned internally and externally at the end of each test.

Figure 3.4
3.6.3. FLAGS
“B”: bubbles in the test
“C”: Clog in the test
“L”: Lower than limits
“H”: Higher than limits
Note: When PLT histogram presents PM alarm, the parameter test value of PDW is ***.
If the test value of WBC is smaller than 0.5x109/L, the instrument will not classify the leukocytes, all
parameter values correlated with WBC will display ***.
3.6.4. HISTOGRAM FLAG
FLAG CAUSE
“R1” means the left part of the lymphocyte 1. RBC hemolysis is not complete,
histogram is abnormal 2. Platelet aggregates,
3. Enormous platelet,
4. Nucleated red blood cell,
“R2” means the area among the lymphocytes 1. Heteromorphic lymphocyte,
and the monocytes is not correctly positioned 2. Big lymphocyte,
3. Eosinophilic,
4. Basophilic,
“R3” means the area between the monocytes 1. Immature granulocytes,
and the granulocytes is not correctly positioned 2. Eosinophilic.
“R4” means the right side of the granulocyte Granulocyte number increases
unusually
“RM”: Many areas exist unusually, possible
reason: The above many kinds of reasons exist
together.
“PM”: Brief on the intersection of platelet and 1. Platelet condense,
red blood cell unusually 2. Big platelet,
3. Small red blood cell,
4. Cell chip and fibrin exist

3.7. Histogram modification
The COUNTENDER 20+ thresholds are automatically calculated for each sample. Nevertheless the
operator can at his convenience modify these thresholds:
1. From the blood cell analyzer window, Select HISTOGRAM in the SETTINGS menu
2. Click PARA to select the Histogram, click LINE to select the line
3. Press LEFT or RIGHT, to adjust the line.
Note: Numeric values are immediately updated.
4. Press RETURN button when the adjustment is completed. COUNTENDER 20+ will spring a
affirm dialog, click CANCEL button, to cancel you adjustment, click OK button to save.
Caution: The improper adjust action may cause incorrect result. Please ensure the necessary of
the action.
Note: When the test result of WBC is less than 0.5 x109/L, the COUNTENDER 20+ will not classify
automatically the WBC.
Figure 3-5
3.8. Turn off

Please carry out the Shut Down procedure after test the blood sample or off duty every day, the
instrument will carry on the maintenance and wash the measuring tube.
The step is as follows:

1. From the blood cell analyzer window click SHUT DOWN
2. Present the SFRI CleanEZ 3.1 for aspiration through the sample probe and press the start
pad.
3. After this cycle the screen shows: “Turn off the instrument now”, turn off the power.
4. Turn off the printer power, clean the workbench and dispose the waste.

Note: Do not turn off the power of instrument directly, without carry on the Shut Down
procedure.

4. Quality Control
Quality Control means the precision, veracity and iteration.

COUNTENDER 20+ proposes 9 QC documents, which can survey 12 parameters.
Choose a QC document firstly, input the reference data.
Warning
Use only SFRI BloodTrol 16 BQC.
Caution:
1. Follow the recommendations to store the controls.
2. Ensure that the control’s are not expire
3. Operate the quality control at fixed time every day.
4.1. Quality control edit

1. From the main window click QC/CALIB > QC > QC EDIT. The following window pops up:
Figure 4-1
2. Choose the QC file you want.

3. Input the batch, period of validity, reference and range data of the calibrator.
4. Click the shortcut key SAVE, save the data of control document.
5. Click the shortcut key DEL, delete the data of control document.
6. Click the shortcut key EXIT, save the data of control document and back to the blood cell
analyzer windows.
4.2. Quality control run

1. After inputting the data of the control, run the quality control and operate as follows:
2. From the main window click QC/CALIB > QC > QC RUN, the instrument turns into QC run
menu, as Figure 4-2 shows.

3. Please follow the instructions of the BQC to run the tests
Figure 4-2
4. Put the controls under the probe, press START key, the indicator LIGHT would glitter, the
analyzer aspirate the calibrator, move the controls away until the indicator LIGHT stop glitter.
5. The result will be displayed at the column of the current serial number when the run is over.
If there is an alarm during the run, the result may be not correct, and then press DEL to
delete this result and test again.
6. Click EXIT button, back to the blood cell analyzer window.
Note: The maximal number of tests stored is 31.
4.3. Levey-Jennings graphs

Through the Levey-Jennings graphs it is possible to check the status of the instrument and to flows
this status day after day.
This type of representation allows the customer to detect very easily and very quickly even a sLIGHT
deviation is the parameters measurement.
From the main window click QC/CALIB > QC > QC GRAPHS, choose the QC file number and the
instrument will display the Levey-Jennings graphs, as Figure 4-3 shows.

Figure 4-3
Each screen can display 4 L-V Charts. These Charts show the batch, time, serial number and
distribution of the QC data. Click the scroll bar at the right of the screen and L-V chart for other
parameters will be displayed.
 The abscissa of the chart is the running time of quality control.

 The above broken line is the upper limit of the range,
 The nether broken line is the lower limit of the normal range.
The three data displayed on the left are: upper value of the normal range, assay value and lower
value of the normal range.
The three data in the right from the top down are: Mean, Diff, CV.
The straight line in the middle of the QC Figure shows the current selected serial number, operate
the scroll bar in the screen can select different serial number, the selected data box below each QC
parameter would show the QC data at the same time, and the time box shows the test time.
The meaning of each control point is as follows: “*”, when it is in the inner of the two broken line,
this point is running normally. Otherwise, it is abnormal. “.”: it means the QC data is wrong or it is
over the display range. Blank means it didn’t have running a quality control.
Click PRINT button to print the quality control data displayed in the screen.
Click EXIT button to go back to the blood cell analyzer windows.
4.4. Quality control table

The system displays the quality control data as a list.
From the main window click QC/CALIB > QC > QC TABLE, the instrument turns into QC table menu
select the quality control file you need, the instrument displays the table of results, as Figure 4-4
shows.
Figure 4-4
The screen displays the 12 QC parameters of 7 groups.

Operate the scroll bar in the screen can select different serial number.
Click PRINT button to print the quality control data displayed in the screen.
Click EXIT button to go back to the blood cell analyzer windows.

5. Calibration
Each instrument have been tested and calibrated strictly before leaving SFRI factory. The
measurement result may be excurse in the daily grind and transportation. Calibration is ensuring the
veracity of the result.
To ensure the instrument’s precision and obtain reliable measured results, the instrument should be
calibrated in these situations:
1. First installation of the instrument or the working environment is changed greatly.
2. Maintenance or service on the instrument.
3. The results of QC are unconventional.
Warning
Only SFRI calibrators “BloodCal” must be used to perform the calibration. Use and store the
calibrators following IFU joint the calibrator.
Make sure the instrument is in perfect working condition before performing calibration.
5.1. Manual calibration

5.1.1. BACKGROUND TEST
Refer to chapter 3.11, Ensure that no malfunction is indicated in the instrument.
5.1.2. CALCULATION OF THE NEW CALIBRATION FACTOR

Run tests of the calibrator. If several tests are performed, calculate the average results to calculate
the new factor.
Calculate the new calibration factor as follows for each measured parameter:
New factor = (Calibrator Target value/ Average result) x old factor
5.1.3. MODIFY THE CALIBRATION COEFFICIENT
1. From the main window click QC/CALIB > CALIBRATION > MANUAL CALIBRATION
Figure 5-1
2. Select the calibration factor you want to modify (Figure 5-1). Modify it

Click PRINT button, if you want to print the current calibration result.
Click OK button to save the current calibration result
Click CANCEL button, to keep the old calibration factor
Note: The input of calibration data that the system allowed is between 70%~130%.
5.2. Auto calibration

Operate as follows:
1. From the main window click QC/CALIB / CALIBRATION / AUTO CALIBRATION
2. Fill up the table with target values of the calibrator (Figure 5-2).
3. Put the calibrator under the probe, press START key, and remove the calibrator when the
LIGHT indicator stops glitter.
4. The result will be displayed in the column of the current serial number when the run is
completed. If there is an alarm during the run, it is possible to delete the last test performed
by pressing DEL.
5. Minimum of 3 runs is required to get average result and calculation of the new calibration
factor.
6. Maximum number of run for calibration is 5
7. Click SAVE button, save the current calibration result.
8. Click EXIT button, back to the blood cell analyzer windows.
Note: The input of calibration data that the system allowed is between 70%~130%. If the result is
over, delete the result and calibrate again.
Figure 5-2

6. Setup
6.1. Time
From the main window click SETTINGS > SETTING > DATE/TIME as in Figure 6-1.
Figure 6-1
As seen in Figure 6-2, click the relative bar to adjust the need item.
Click OK, to save the new parameters and to go back to the blood cell windows.
Click CANCEL, the system will not save the new parameters.
Figure 6-2

6.2. Printout
From the main window click SETTINGS > SETTING > PRINTOUT.
Figure 6-3
The Printout window opens as Figure 6-4 below shows
Figure 6-4
Click the drop down you want to adjust, it will drop down a selection window.
Click the item you want to change.
Click OK, save the new parameters and back to the blood cell windows.
Click CANCEL, the system will not save the new parameters and will go back to the blood cell
window.
Printout
Set up as “Recorder”: data will be printed out through the built in thermal printer
Set up as “Printer”: data will be printed out through an external printer if connected.
Set up as “Net”: data will be sent to the LIS.
Set up as “Recorder, net”: data will be printed out through the thermal printer & sent to the LIS.

Set up as “Printer, net”: data will be printed out through the external printer & sent to the LIS.
Report
By using the built in thermal printer different printouts are available
“Short”: numeric results without histograms
“Long”: numeric results and histograms
“Landscape short”: numeric results without histograms in landscape
“Landscape long”: numeric results and histograms in landscape
Username
Printer model
Select the correct printer driver.
Note: Only dot matrix printers can be plugged in to the COUNTENDER 20+ instrument.
6.3. Configuration
From the main window click SETTINGS > SETTING > CONFIG
Figure 6.5

The instrument brings up the “Configuration” window:
Figure 6-6
Click the box you want to adjust; it will drop down a selection window.
Click the item you want to change.
Click OK, save the new parameters and back to the blood cell windows.
Click CANCEL, the system will not save the new parameters and back to the blood cell windows.
Auto
Set the print setup as “ON”; the system will print reports automatically after each test.
Set the dormancy setup as “Off”; the system will remain in working status all along. Set the stand-by
mode as defined data, when the time of the instrument have no operation exceed the defined data,
the instrument turn into stand-by mode, parts of the circuits will be turned off, the probe is take back
in the inner of instrument. This status can retrench electronic power, extend the use time of
instrument. By pressing any mouse key the stand-by mode is canceled.
Set the rinse setup as “OFF”, the system would not clean automatically. Set it as a data, when the
running time is over the set data, the system would run a rinse. It can keep the measure circle clean,
prevent the instrument from malfunction.
Alarm
Set the alarm as “OFF”, the instrument would not alarm when it has problem, it only shows the
information at the information section. Set the alarm as “ON”, the instrument would alarm when it
has problem, and shows the information at the information section.
Set the prompt as “OFF”, the instrument would not have prompt voice when the test finished. Set
the prompt as “ON”, the instrument would have prompt voice as “Di-” when the test finished.
Display
Set the reference data as “No shows”, the parameter section of the windows would not shows the
reference. Set the reference data as “L-H”, the parameter would shows in the information from
lower data to higher data. Set the reference data as “H-L”, the parameter would shows in the
information from higher data to lower data.

6.4. Reference ranges
From the main window click SETTINGS > SETTING > LIMITS the instrument pops up the reference
range setting window.
There are 8 reference groups available. A dot before a reference group means the relevant group has
been selected.
Select the reference group you want to modify.
Click DEFAULT and the instrument will reset with the pre-registered values.
Click the adjust bars of the limit boxes to adjust the upper and lower limits of each reference in the
group.
The function of menu and shortcut keys:

SAVE: Saves the reference data of the current group.
PRINT: Prints the reference data of the current group.
MUTE: Gets rid of the malfunction alarm.
HELP: Opens the Help window, obtain the help on line.
EXIT: Quits current windows and goes back to blood cell analyzer window.
Figure 6-7
Note: The reference ranges may not suitable for the location. User can modify them at his
convenience.
Caution: The change of the normal ranges could cause the abnormal prompt of the hematology
index Ensure the change of the reference data is correct.
6.5. Operator & Department information

In this part of the menu you can preset operators and prescribers names as well as department
information.
From the main window click SETTINGS > SETTING > OPERATOR the instrument will display:

Figure 6-8
6.5.1. ADD DEPARTMENT

Click ADD, Figure 6-6 is displayed.
Input the department name and abbreviation.
Click CANCEL, the current input information will be canceled, system back to the department set up
windows.
Click OK, save the current input information, system back to the department set up windows.
Note: The abbreviation of the relevant department should be easy to memorize.
Figure 6-9
6.5.2. MODIFY DEPARTMENT INFORMATION

Click the serial number you want to change, then click MODIFY, the system will open the “Modify”
windows, as show on Figure 6-7.

Figure 6-10
Modify the information you want to change.

windows.
6.5.3. DELETE DEPARTMENT INFORMATION

Click the serial number you want to modify, then click DELETE, the selected department information
will be deleted.
6.5.4. PRINT DEPARTMENT INFORMATION

Click PRINT; print all the department information stored.
Figure 6-11
6.5.5. ADD DOCTOR INFORMATION

Click ADD, Figure 6-9 is displayed.
Input the doctor’s name and abbreviation. Assign a department to the doctor.
windows.

Figure 6-12
6.5.6. MODIFY DOCTOR INFORMATION

Click the serial number you want to change, then click MODIFY, Figure 6-10 is displayed.
Figure 6-13
Modify the information of the doctor you want to change.

windows.
6.5.7. DELETE DOCTOR INFORMATION

Click the serial number you want to modify, then click DELETE, the selected department information
will be deleted.
6.5.8. PRINT DOCTOR INFORMATION

Click PRINT, print all the doctors information stored.

7. Review
The instrument stores all the tests data automatically. Users can inquire, modify, print and delete the
data from the Datalog menu.
7.1. Datalog
From the main window click DATALOG the instrument pops up the following:
Figure 7-1
This window shows the test data in the form of list.

Each page shows data of 8 tests. The arrows on the scroll bar under the screen shows the current
position.
Meaning of characters:
“H” means result is higher than reference data.
“L” means result is lower than reference data.
“C” means it have clog malfunction in the detection.
“H” means it have bubble malfunction in the detection.
7.1.1. REVIEW THE SAMPLE DATA

Clicking the left button of the scroll bar under the screen moves back through the data.
Clicking the right button of the scroll bar under the screen moves ahead through the data.
7.1.2. DELETE DATA OPERATE

Data can be deleted as follows:
Click the button of the data number, it would shows as reverse video, if click again, cancel the
choose.
Choose Delete in the menu, it will spring a dialog box, user can choose delete the data or not.
Choose OK, the data will be delete, choose cancel, the operation will be cancelled.

7.1.3. DELETE ALL DATA OPERATE
1. Right click the mouse and then choose ALL SELECT. A window will open where users can
choose to delete the data or not. Choose OK and all data will be deleted. Choose CANCEL
and the operation will be cancelled.
2. Right click the mouse and then choose DELETE. A window will open where users can choose
to delete all the data or not. Select OK and the data will be deleted. Select CANCEL and the
operation will be cancelled
7.1.4. PRINT AS LIST OPERATE AS FOLLOW STEPS

Click the button of the data number, it would shows as reverse video, re-click, cancel the choice.
Click the shortcut key PRINT, the selected data would be printed a list.
7.1.5. INQUIRE PRINT THE SELECT DATA AS FOLLOW STEPS

Right click the mouse, choose INQUIRE in the menu and a window will open as in Figure 7-2.
Choose the item you want to inquire about by selecting the box in front of it. The “X” in the box
means the item has been selected, input the key word you want to inquire.
Figure 7-2
Function of menu and shortcut keys:

All select: select or resume all the sample data.
Detail: turn into the detail review windows.
Inquire: Inquire the data with discretional data.
Delete: Delete the selected data.
Print: Print the data as list.
Mute: Get rid of the malfunction alarm.
Help: turn into help windows, obtain the help on line.
Back: Quit currently windows, back to blood cell analyzer windows.
7.2. Detail review

From the detail review window, choose the detail by menu or shortcut key, it will turn into detail
review window, as shown on Figure 7-3.
This window shows sample data as graphics.
Quick review
Click BACK key, review the back data.

Click FRONT key, review the front data.
Material Modification
Click shortcut key DATA, it will open an Edit window; operate it according to section 3.6.1.
Data modification
Click right key of the mouse, choose ADJUST in the menu. Operate as follows:
Figure 7.3
1. Click shortcut key para, can select WBC, RBC, PLT in turn.
2. Click shortcut key Line, can select the class line in the histogram.
3. Click shortcut key right or left, can move the class line right or left. The data calculated
according the new class line would shows at the left data section.
4. Click shortcut key Back, it will spring a dialog box, user can choose save the modification
result or not.
5. Click OK, the result will be saved,
6. Click CANCEL, the result would not be saved and the windows is exited.
Function of menu and shortcut keys:

FRONT: review the front data.
Back: review the back data.
Edit: modify the data.
Print: Print the data as list.
Mute: Get rid of the malfunction alarm.
Help: turn into help windows, obtain the help on line.
Back: Quit currently windows, back to blood cell analyzer windows.

8. Service
This chapter explains several functions of instrument that can help users operate the instrument
conveniently and friendly.
On the blood cell analyzer window, right click the mouse, choose FUNCTION/SERVICE.
8.1. Flush
This function is used to solve the usual probe clog.
There will be a fixed pressure and voltage effect to the probe to get rid of the clog.

8.2. Enhanced flush
This function is used to solve stubborn probe clog.
From the main menu, right click and select ENHANCED FLUSH. Present SFRI Clair 3.1 and press the
START button of the instrument to launch the program.
8.3. Cleaning
This function is used to clean probe, chambers and measure tube routinely.

8.4. Enhanced cleaning
This function is used to clean the probe and chambers and to measure tube enhancive.
From the main menu, right click and select ENHANCED CLEANING. Present SFRI CleanEZ 3.1 and
press the START button of the instrument to launch the program.
8.5. Drain chambers

This function is used for observe the count pool and get rid of the liquid in count pool.

8.6. Park
Before transportation or if the instrument is not going to be used for 2 weeks or longer, use this
function to clean the instrument. Prepare distilled water (approx 500 ml) then follow the step by step
instructions displayed on the COUNTENDER 20+’s screen.
8.7. Prime diluent

Fill dilution to relevant tube.

8.8. Prime lyse
Fill lyse to relevant tube.
8.9. Prime rinse

Fill Rinse to relevant tube.

8.10. Weekly maintenance
Users can finish the weekly maintenance following the screen prompt.
Prepare SFRI Cleaner 3.1 and SFRI Clair 3.1 first, and then follow the instructions displayed on the
COUNTENDER 20+’s screen.
8.11. Mechanic
Click SERVICE > MECHANIC
The password is: 6100.

The instrument turn into detection windows, valves, motors, circuits and connectors will be check to
detect any malfunction.
Valve
“ON” means the valve is on.
“OFF” means the valve is off.

Motor
“OK” means the motor is in the course of nature.
“FAIL” means the motor is failure.
Circuit
“OK” means the circuit is in the course of nature.
“FAIL” means the circuit is failing.
“OFF” means there is no negative pressure.
“ON” means there is negative pressure.
“LOW” means the temperature is lower than 18°C.
Functions of menu and shortcut keys:

MUTE: Close the alarm voice.
HELP: turn into help windows, obtain the help on line.
BACK: Quit currently windows, back to blood cell analyzer windows.
8.12. Special
The instrument opens a special maintenance window.
This function must be operated by qualified technicians certified by SFRI Sarl.

9. Maintenance
COUNTENDER 20+ requires minimum maintenances. Nevertheless this maintenance has to be

performed according to the schedule hereafter.
Performing the appropriate maintenance allows the instrument to stay in good condition and
decreases risks of malfunction.
This chapter includes methods for protection and maintenance. For any further information please
contact your local distributor.
9.1. Routine maintenance

External cleaning
Clean up regularly COUNTENDER 20+ with neuter detergent or distilled water.
Caution: Only use the neuter detergent. Never use corrosive acids and alkali, volatile organic
solvent such as: acetone, ether chloroforms to clean it up.
9.2. Daily maintenance

There are two types of daily maintenance:
1. Automatic cleaning can be set depending from the number of tests performed per day and
the time of use.
Work time > 8 hours, auto-clean time=4 hours;
Work time > 4 hours, auto-clean time=4 hours;
These delays are given for normal operating conditions. Your local distributor can advise you
to perform the auto cleaning program more frequently
2. Shutdown When shutdown the instrument, it will run the shutdown auto clean procedure.
9.3. Weekly maintenance

This instrument has a weekly maintenance procedure.
Proceed as follows:
Click SERVICE > MAINTAIN program. Present SFRI CleanEZ 3.1 and press the start pad of the
COUNTENDER 20+ to launch the program.
9.4. Monthly maintenance

Clean the dust monthly.
Operate as follows:
1. Turn off the power supply, pull out the power line.
2. Hold up the air filter cover with screwdriver.
3. Take off the cover and clean them with neuter detergent with brush and airing them. Brush
away the dust cling at the air filter grid in the back of instrument.
Note: Only use the neuter detergent clean the air filter and airing it without heat.

9.5. Yearly maintenance
According the requirement, the maintenance should be performed by a service engineer appointed
by SFRI Sarl. Please contact your local distributor for performing the yearly maintenance.
9.6. Maintenance
Before transportation or storage for many weeks the COUNTENDER 20+ Please perform the following
procedures:
1. Perform the park program
2. Close bottles of reagents and store them. User should perform efficient action to prevent the
material from deteriorating and misusing.
3. Pull out diluent tubing, rinse tubing, lyse tubing and waste tubing and clean them with
distilled water. Dry them in shady place and then pack with plastic.
4. Screw the stopples to the tube connectors.
5. Pull out the power wire.

10. Troubleshooting
This chapter gives instructions for identifying, troubleshooting, and correcting instrument problems.
If you cannot solve the problems according to this guidance or you wish to get more information,
please contact your local distributor.
SYMPTOMES CAUSES ACTIONS

No start up 1. Check if there is power or not.
2. Check if the power socket is loose.
3. Check if the fuse is blown. If the fuse is blown,
replace it as follows: The fuse is installed in the fuse-
box at the side of power switch.
Fuse specification: V 250V 2A
Warning
Only use the appointed fuse.
Waste container is Empty the trash can.
full
No diluent 1. Change the SFRI Diluent 3.1.
2. From the blood cell analyzer window, click
FUNCTION > SERVICE > PRIME DILUENT 2 to 3
times
No lyse 1. Change the SFRI Lyse 3.1.
FUNCTION > SERVICE > PRIME LYSE 2 to 3 times
No rinse 1. Change the SFRI Cleaner 3.1.
FUNCTION > SERVICE > PRIME RINSE 2 times
WBC clog or RBC If the count time exceeds upper limit during
clog measurement, following error message will be
displayed accordingly:
“WBC clog” or “RBC clog”
1. In blood cell analyzer main window, press FLUSH.
2. For pertinacity jam, perform follow procedures if
step 1 can’t solve problem.
3. In the blood cell analyzer window, Click SERVICE >
ENHANCED FLUSH. Follow the instructions
displayed on the screen step by step.
WBC bubbles or If the count time under lower limit during
RBC bubbles measurement, following error message will be
displayed accordingly:
“WBC bubbles” or “RBC bubbles”
In blood cell analyzer main window, click SERVICE >
RINSE in the menu. Follow the instructions
displayed on the screen step by step.
HGB failure or 1. In blood cell analyzer windows, Click SERVICE >
bubbles RINSE in the menu.
2. Perform following procedures if step 1 can’t solve
problem.
In blood cell analyzer windows, click SERVICE >

ENHANCED RINSE in the menu. Follow the
instructions displayed on the screen step by step.
Recorder has no 1. Gently press the recorder door to open it.
paper 2. Insert the new paper being to the paper mouth,
let the print side towards the thermal head
3. Pull the paper in the other side of the printer,
keep it straight.
4. Put the paper across the recorder door.
5. Close recorder door.
Printer The thermal head is too hot.
temperature too Pause for 5 minutes.
high « Recorder
temp over »

11. Help
The integrated help system can help users solve most problems they come across. It also presents
the operation method.
The Help appears in a separate window and users can easily and conveniently read and search
contents.
On the blood cell analyzer window, press the right key of the moue, choose Help in the menu, the
help window will be brought up as shown on Figure 11-1.
Figure 11-1
This help window shows contents by chapter so users can easily find the help points they are looking
for.
Use the scroll bar on the right to move up and down the contents to read the help information.
Functions of menu and shortcut key:

SELECT: choose the contents you want.
MUTE: Turn off alarm sound.
BACK: Quit current help window and go back to blood cell analyzer main window.

Appendices – TSB
Appendix 1: Safety Symbols

SYMBOL WARNING CONDITION ACTION
Biohazard: Consider all materials Wear standard laboratory attire,
(specimens, reagents, controls, glove and follow safe laboratory
calibrators, or components that contain procedures when handling any
or have contacted human blood) as being material in the laboratory.
potentially infectious.
Electrical Shock Hazard: The electrical Before continuing, unplug the BA-88
warning icon alerts users to the possibility analyzer from the electrical outlet.
of electrical shock in the described
activity or at the posted location.
Probe Hazard: The probe may contain Avoid any unnecessary contact with
biohazard materials, including controls the probe and probe area.
and calibrators.
NOTE: Indicates to the operator
information of importance in the
procedure to be performed. This heading
is also used to indicate specific sample
handling techniques that are helpful in
operating the instrument.
CAUTION: Indicate to the operator
information of importance that could
result in damage to the instrument or
affect the test due to improper operation
if these instructions are not followed.
WARNING: Indicate to the operator
information regarding potential hazards
that may cause personal harm to the
operator if these instructions are not
followed.

Appendix 2: Reagents and consumables list
REAGENTS REFERENCE VOLUME
SFRI Diluent 3.1 HSL321 20 L
SFRI Lyse 3.1 HSL302 500 ml
SFRI Cleaner 3.1 HSG305 5L
MAINTENANCE SOLUTIONS REFERENCE VOLUME

SFRI CleanEZ 3.1 HSC302 60 ml
SFRI Clair 3.1 HSC102 60 ml
CONTORLS REFERENCE QUANTITY

BloodTrol 16 - 3 tubes N R021005 1
BloodTrol 16 - 3 tubes LNH R021001 1
BloodTrol 16 - 6 tubes N R021002 1
BloodTrol 16 - 6 tubes LNH R021006 1
CALIBRATOR REFERENCE QUANTITY

BloodCal – 1 tube R021003 1
CONSUMABLES REFERENCE QUANTITY

Thermal paper C010001 1
Ecouvillon cotton C010002 10


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Revision History

Version Date Modification

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 3

3. Sample analysis .................................................................................31

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6 | COUNTENDER 20+_Operator Manual_ENG_V12.00

11. Help .....................................................................................................69

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 7

Content of this manual is subject to changes without prior notice.

Responsibility on the Manufacturer Party

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Workmanship and Materials

Safety, Reliability and Performance

10 | COUNTENDER 20+_Operator Manual_ENG_V12.00

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 11

ITEMS ABBREVIATION UNITS

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 13

Type Name Code

Type Name Code

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 15

Parameters given by histogram

Volume per test

16 | COUNTENDER 20+_Operator Manual_ENG_V12.00

Linearity and precision

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 17

18 | COUNTENDER 20+_Operator Manual_ENG_V12.00

Thermal printer Start Key

1.4. Rear view

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 19

WBC graph section

PLT graph section RBC graph section Display section

Auxiliary operation section System time section

20 | COUNTENDER 20+_Operator Manual_ENG_V12.00

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 21

22 | COUNTENDER 20+_Operator Manual_ENG_V12.00

1.6. Detection principle

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 23

1.6.2. MEASUREMENT PRINCIPLE OF HGB

1.6.3. RED BLOOD CELL VOLUME

1.6.4. METHODS FOR PARAMETERS MEASUREMENT

Measured WBC, RBC, PLT, HGB, MCV, MPV.

24 | COUNTENDER 20+_Operator Manual_ENG_V12.00

AL: Number of cell in area of LYM;

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 25

2.2. Pre-installation checks

2.3. Installation requirements

2.3.2. SPACE REQUIREMENTS

Note: put reagents at the same height.

2.3.3. POWER REQUIREMENTS

2.4. Reagents connections

2.4.1. LYSE CONNECTION

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 27

2.4.2. DILUENT CONNECTION

2.4.3. RINSE CONNECTOR

2.4.4. WASTE CONNECTOR

2.5. Recorder paper installation

2.6. Keyboard and mouse installation

2.7. Printer installation (Optional)

28 | COUNTENDER 20+_Operator Manual_ENG_V12.00

Note: plug printer cable into a socket marked in rear panel.

2.8. Barcode scanner installation (Optional)

2.9. Power cable connection

COUNTENDER 20+_Operator Manual_ENG_V12.00 | 29

3.1. Before analysis