13253.

BIOLOGIA CELULAR E MOLECULAR

Biologia celular e molecular / Coord. Carlos Azevedo. - 4. ed. - Lisboa : Lidel,

2005. - XXII, 587 p. - Clique aqui para saber mais sobre o livro :
http://www.fca.pt/lidel_index2.html

ISBN 13:978-972757354-7 : 29.40 EUROS

Biologia celular / Biologia molecular

CDU 576

576 BIO (ESTGFR) - 95/08. -

13279. QUALITY TRAITS OF SUCKLING-LAMB MEAT COVERED BY THE

PROTECTED GEOGRAPHICAL INDICATION "LECHAZO DE CASTILLA Y
LEON" EUROPEAN QUALITY LABEL

Quality traits of suckling-lamb meat covered by the protected geographical

indication "Lechazo de Castilla y Leon" European quality label / Ana Cristina
Oliveira Lopes Figueira...[et al.] . - Clique aqui para acesso ao texto integral
(restrito à B-on) : http://dx.doi.org/10.1016/j.smallrumres.2008.02.002 . - The
quality of suckling-lamb meat (derived from Churra, Castellana and Ojalada
breeds), covered by the protected geographical indication (PGI) "Lechazo de
Castilla y Leon", and the effects of carcass weight (CW), breed and sex on
quality were studied. The edible portion (EP) of 81 carcasses was analysed for
proximate composition, fatty acid, cholesterol, amino acid and mineral
contents. The gissimus thoracis and lumborum muscle (30 samples) were
analysed for proximate composition, pH, myoglobin content, water holding
capacity, Warner-Bratzler shear force, and total and soluble collagen content.
This study contributes to characterization of suckling-lamb meat quality
recognised in the European Union with a PGI label and provides new data on
the composition of the EP of the carcasses. CW and breed had a significant
effect on several quality traits, most related to fatness
In: Small Ruminant Research : Elsevier. - 77, Issue 1, June 2008, p. 65-70

Carnes

CDU 664.9

664.9 QUA (ESTGFR) - 120/08

13282. EFFECT OF REARING SYSTEM ON SOME MEAT QUALITY TRAITS

AND VOLATILE COMPOUNDS OF SUCKLING LAMB MEAT

Effect of rearing system on some meat quality traits and volatile compounds of
suckling lamb meat / Ana Cristina Oliveira Lopes Figueira...[et al.] . - Clique
aqui para acesso ao texto integral (restrito à B-on) :
http://dx.doi.org/10.1016/j.smallrumres.2008.03.015. - Twenty Churra-breed
suckling lamb carcasses from two groups of animals were used in this study.
One group had been reared on maternal milk (MM), and the other had been
reared on milk replacer (MR) until slaughter at 25-35 days old. The effects of
the type of milk on several meat quality traits were studied. These effects
included pH, colour, WHC, texture, retinol and tocopherol concentrations,
colour and lipid oxidation stability, and the volatile compounds that formed
during boiling of the meat. Furthermore, a sensory analysis (triangle test) was
carried out. The colour of M. longissimus dorsi of MM samples showed higher
L*, lower a*, and higher b* values than those of MR samples (P 0.05). Retinol,
a-, d- and ?-tocopherol levels were all higher in the meat of lambs reared on
MM (P 0.001). Rancimat tests and TBARS analysis revealed more lipid-
oxidative stability for the meat of the MR group, and the colour of meat from
this group was also more stable. Likewise, volatile compounds derived from
lipid oxidation were more abundant in the MM samples than in the MR group
samples. The presence of volatiles attributed to non-oxidative lipid thermal
degradation also differed between the two rearing systems, with concentrations
of volatiles derived from dodecanoic acid being clearly higher for the MR meat
samples. Residues of butylated hidroxytoluene (BHT) were detected in MR
samples but not in the samples of the MM treatment. In the triangle test, an
untrained panel could not detect a significant difference between MM and MR
meat samples. The present study has demonstrated that variation in the
composition of milk sources (MM vs. EM) used in rearing suckling lambs may
be responsible for a significant effect in oxidative stability of fresh suckling lamb
meat during storage and display and in the volatile composition of cooked
suckling lamb meat. Thus, meat from MR-fed suckling lambs may become
more stable to oxidation compared to meat from MM-fed suckling lambs. MR-
reared meat may, however, have a different flavour from what is expected from
the more traditional MM-fed suckling lamb meat.
In: Small Ruminant Research : Elsevier. - 78, Issues 1-3, August 2008, p.1-12

Carnes

CDU 664.9

664 EFF (ESTGFR) - 121/08

13288. FATTY ACID COMPOSITION IN SUBCUTANEOUS,

INTERMUSCULAR AND INTRAMUSCULAR FAT DEPOSITS OF SUCKLING
LAMB MEAT: EFFECT OF MILK SOURCE

Fatty acid composition in subcutaneous, intermuscular and intramuscular fat

deposits of suckling lamb meat: Effect of milk source / Ana Cristina Oliveira
Lopes Figueira...[et al.] . - Clique aqui para acesso ao texto integral (restrito à
B-on) : http://dx.doi.org/10.1016/j.smallrumres.2006.12.005. - A total of 20
Churra suckling lambs from a sheep dairy farm were divided into two groups
and reared on ewes' milk (EM) or milk-replacer (MR). The suckling lambs were
slaughtered at ages between 25 and 35 days. Fatty acid profiles of milk source
fat as well as subcutaneous (SC), intermuscular (IN) and intramuscular (IR) fat
of lamb carcasses were determined, and the effect of the rearing system
investigated. As regards fat deposits, proportions of polyunsaturated (PUFA),
saturated (SFA) and monounsaturated (MUFA) fatty acids showed high
similarity between IN and SC fat, for either of EM or MR samples. However,
and only for MR samples, IN fat had lower odd-chain fatty acid (OCFA) and
branched-chain fatty acid (BCFA) contents than SC fat. In IR fat, when
compared to the other fat deposits, significant differences in all PUFA contents,
except for c9t11-C18:2, were found. As for rearing system, a significant effect
of dietary fat on the contents of the majority of fatty acids for each fat deposit
was observed. Differences in fatty acid contents between the two milk source
fats resulted in concomitant differences for those fatty acids in the carcass fat
deposits, with the exception of C18:2 n-6, C20:4 n-6, C18:3 n-3 and c9t11-
C18:2. Both the n-6 and n-3 fatty acid contents appeared to be influenced by
the dietary n-6/n-3 ratio. Meanwhile, c9t11-C18:2 content was slightly higher in
MR carcass fat samples but lower in MR fat. A higher presence of some
specific trans-C18:1 fatty acids in MR fat than in EM fat which can be
precursors of c9t11-C18:2, might have resulted in a higher endogenous
synthesis of c9t11-C18:2, via ?9-desaturase.
In: Small Ruminant Research : Elsevier. - 73, Issues 1-3, November 2007,
p.127-134

Carnes

CDU 664.9

664.9 FAT (ESTGFR) - 127/08

13300. VIEIRA, Maria Margarida Cortês

Optimization of a cupuaçu (Theobroma Grandiflorum) nectar formulation /

Margarida C. Vieira, Cristina L. M. Silva. - Clique aqui para acesso ao texto
integral (restrito à B-on) : http://dx.doi.org/10.1111/j.1745-4530.2004.tb00629.x
. - A Cupuaçu (Theobroma grandiflorum) nectar formulation was optimized, for
European consumers, using an augmented simplex centroid mixture design.
the working ranges, x1 (Sugar, 10-25%), x2 (Cupuaçu, 15-30%) and x3 (Water,
60-75%), were based on a common Brazilian recipe. As responses, a sensory
panel evaluated taste, acidity, sweetness, viscosity, mouthfeel and overall
opinion. For each response, the best mathematical predictive model was
chosen, based on Response Surface statistical analysis. an optimization was
then performed, and the optimum was Cupuaçu (18.5%), Sugar (11%) and
Water (70.5%). Tolerance tests were carried out and, although differences
were detected (a= 5%), the level of acceptance remained above average
In: Journal of Food Process Engineering. - Trumbull : Food & Nutrition. - Vol.
27, nº. 3 (2004), p. 181-196

Biotecnologia / Conservação de alimentos

CDU 66.098

66.098 VIE Opt (ESTGFR) - 140/08

13301. THERMAL INACTIVATION OF ALICYCLOBACILLUS

ACIDOTERRESTRIS SPORES UNDER DIFFERENT TEMPERATURE,
SOLUBLE SOLIDS AND PH CONDITIONS FOR THE DESIGN OF FRUIT
PROCESSES

Thermal inactivation of Alicyclobacillus acidoterrestris spores under different

temperature, soluble solids and pH conditions for the design of fruit processes /
Filipa M. Silva...[et al.]. - Clique aqui para acesso ao texto integral (restrito à B-
on) : http://dx.doi.org/10.1016/S0168-1605(99)00103-8. - Alicyclobacillus
acidoterrestris, a thermoacidophilic, non-pathogenic and spore-forming
bacterium has been detected in several spoiled commercial pasteurised fruit
juices. A. acidoterrestris spores, besides being resistant to the pasteurisation
treatment conditions normally applied to acidic fruit products, can germinate
and grow causing spoilage. Therefore, this microorganism was suggested as
the target to be used in the design of adequate pasteurisation processes. The
objectives of this work were to investigate the influence of temperature (T: 85-
97°C), total soluble solids (SS: 5-60°Brix or % by weight) and pH (2.5-6.0) on
D-values (decimal reduction time) of Alicyclobacillus acidoterrestris (type strain,
NCIMB 13137) spores, and to fit a model using response surface methodology.
A central composite face-centred experimental design was used, and the
response, D-value determined in malt extract broth, ranged between
0.498±0.045 and 94.9±6.7 min. Within the factor ranges studied, temperature
was the parameter that most affected the D-value. Following this was the SS
and, lastly, the pH value. A linear decrease in D-value was observed with
decreasing SS and pH, and a non-linear decrease in D-value was noticed with
increasing temperature. A second order polynomial was successfully fitted to
the data (R2=0.98). In general, D-values measured in real fruit systems, such
as orange, apple and grape juices, blackcurrant concentrates, cupuaçu (exotic
fruit) extract and orange juice drink, were higher than those predicted by the
malt extract broth model. This result emphasises the importance of
experimental validation of any model-derived process.
In: International Journal of Food Microbiology. - New York : Elsevier. - Vol. 51,
nº. 2-3 (1999), p. 95-103

Biotecnologia / Conservação de alimentos / Conservação pelo calor /

Alicyclobacillus acidoterrestris

CDU 66.098
CDU 579.2

66.098 The (ESTGFR) - 141/08

13302. CRUZ, Rui M. S.

Effect of heat and thermosonication treatments on peroxidase inactivation

kinetics in watercress (Nasturtium officinale) / Rui M. S. Cruz, Margarida C.
Vieira, Cristina L. M. Silva. - Clique aqui para acesso ao texto integral (restrito
à B-on) : http://dx.doi.org/10.1016/j.jfoodeng.2004.11.007. - The effect of heat
and the combined heat/ultrasound (thermosonication) treatment on the
inactivation kinetics of peroxidase in watercress (Nasturtium officinale) was
studied in the temperature range of 40-92.5 °C. In the heat blanching
processes, the enzyme kinetics showed a first-order biphasic inactivation
model. The activation energies and the rates of the reaction at a reference
temperature for both the heat-labile and heat-resistant fractions were,
respectively, Ea1 = 421 ± 115 kJ mol-1 and Ea2 = 352 ± 81 kJ mol-1, and . The
initial relative specific activity for both isoenzyme fractions were also estimated,
being C01 = 0.5 ± 0.08 µmol min-1 mg protein-1 and C02 = 0.5 ± 0.06 µmol
min-1 mg protein-1, respectively. The application of thermosonication was
studied to enable less severe thermal treatments and, therefore, improving the
quality of the blanched product. In this treatment the enzyme kinetics showed a
first-order model. The activation energy, the rate of reaction at a reference
temperature and the initial relative specific activity were, respectively, Ea3 =
496 ± 65 kJ mol-1, and C03 = 1 ± 0.05 µmol min-1 mg protein-1, proving that
the enzyme became more heat labile. The present findings will help to design
the blanching conditions for the production of a new and healthy frozen
product, watercress (Nasturtium officinale), with minimized colour or flavour
changes along its shelf life.
In: Journal of Food Engineering. - New York : Elsevier. - Vol. 72, nº. 1 (2006),
p. 8-15

Biotecnologia / Conservação de alimentos

CDU 66.098

66.098 CRU Efe (ESTGFR) - 142/08

13303. ALICYCLOBACILLUS ACIDOTERRESTRIS SPORES AS A TARGET
FOR CUPUAÇU (THEOBROMA GRANDIFLORUM) NECTAR THERMAL
PROCESSING: KINETIC PARAMETERS AND EXPERIMENTAL METHODS

Alicyclobacillus acidoterrestris spores as a target for Cupuaçu (Theobroma

grandiflorum) nectar thermal processing: kinetic parameters and experimental
methods / Margarida C. Vieira...[et al.]. - Clique aqui para acesso ao texto
integral (restrito à B-on) : http://dx.doi.org/10.1016/S0168-1605(02)00043-0. -
The kinetic parameters of thermal inactivation of a spore former,
Alicyclobacillus acidoterrestris, in a tropical fruit nectar [25% of Cupuaçu
(Theobroma grandiflorum) pulp and 15% sugar] were determined by the
isothermal method (IM), under batch heating, and by the paired equivalent
isothermal exposures (PEIE) method, under non-isothermal continuous
conditions.The isothermal experiments were repeated three times, every 4
months, with the same spore suspension kept frozen between experiments.
The aging of spores, under frozen storage, seemed to produce a notorious
increase in the z-value from experiment to experiment: Experiment 1
(z=7.8±2.6 °C, D95 °C=5.29±0.96 min), Experiment 2 (z=22±5 °C, D95
°C=5.99±0.63 min), and Experiment 3 (z=29±10 °C, D95 °C=3.82±0.48 min).
The evaluation of the kinetic parameters by the PEIE method was carried out in
parallel with Experiment 3, with the same aged spores, and the results (z=31±6
°C, D95 °C=5.5±1.2 min) were close to the ones obtained in this experiment.
From this work, it seems that the PEIE method can also be applied to evaluate
the reduction parameters of a spore-forming microorganism, and in a more
realistic way, since the continuous system eliminates the errors caused by
come-up and cool-down times (CUT and CDT) that are unavoidable in
isothermal experiments. Therefore, when designing a thermal process for a
continuous system, the PEIE method should be used, or the chances are that
the process would be underdesigned, risking that the desired level of spore
inactivation would not be achieved. An optimization of the thermal processing
conditions was next performed for Cupuaçu nectar, considering a 5D reduction
in A. acidoterrestris spores. If a pasteurization process is considered, the
conditions that ensure safety (9 min at 98 °C) only allow a 55% retention of
ascorbic acid (AA). If sterilization is considered, 8 s at 115 °C will ensure a safe
product and retain 98.5% of the original ascorbic acid. Therefore, if A.
acidoterrestris is considered as the target microorganism, the nectar should
undergo an aseptic high temperature short time principle (HTST) process to
achieve a 5D reduction in this acidophilus spore former. However, if the hot-fill-
and-hold pasteurization process is preferred, the product should be fortified
with ascorbic acid.
In: International Journal of Food Microbiology. - New York : Elsevier. - Vol. 77,
nº. 1-2 (2002), p. 71-81

Biotecnologia / Conservação de alimentos / Alicyclobacillus acidoterrestris /

Cupuaçu

CDU 66.098

66.098 Ali (ESTGFR) - 143/08

13304. CRUZ, Rui M. S.

Modelling kinetics of watercress (Nasturtium officinale) colour changes due to

heat and thermosonication treatments / Rui M. S. Cruz, Margarida C. Vieira,
Cristina L. M. Silva. - Clique aqui para acesso ao texto integral (restrito à B-on)
: http://dx.doi.org/10.1016/j.ifset.2007.01.003. - Watercress (Nasturtium
officinale) colour changes due to blanching by heat and a combined treatment
of heat/ultrasound (thermosonication) were studied in the temperature range of
82.5 to 92.5 °C. The application of thermosonication was intended to enable
less severe blanching treatments and, therefore, improve the quality of the
blanched product. The thermosonication blanching processes promoted
changes of the green colour (an parameter) at a higher rate (P 0.05), when
compared with the heat blanching processes. No significant differences (P
0.05) were detected between heat and thermosonication blanching processes
in terms of the colour parameters Ln, bn and TCD changes. In both treatments,
a fractional first order model fitted well the experimental data for Ln, an and bn
(RH2 0.05) between thermally treated and thermosonicated watercress
samples.The present findings will help to evaluate the effectiveness of
thermosonication as a novel process to replace the classical heat treatment.
In: Innovative Food Science and Emerging Technologie. - New York : Elsevier.
- Vol. 8, nº. 2 (2007), p. 244-252

Biotecnologia / Conservação de alimentos

CDU 66.098

66.098 Mod (ESTGFR) - 145/08

13305. CRUZ, Rui M. S.

Effect of heat and thermosonication treatments on watercress (Nasturtium

officinale) vitamin C degradation kinetics / Rui M. S. Cruz, Margarida C. Vieira,
Cristina L. M. Silva. - Clique aqui para acesso ao texto integral (restrito à B-on)
: http://dx.doi.org/10.1016/j.ifset.2007.10.005. - Article in Press, Corrected
Proof - Note to users . - The use of ultrasound in food processing creates novel
and interesting methodologies, which are often complementary to classical
techniques. In this work, the effect of heat and the combined treatment
heat/ultrasound (thermosonication) on the thermal degradation kinetics of
vitamin C in watercress (Nasturtium officinale) was studied in the temperature
range of 82.5 to 92.5 °C. First order reaction kinetics adequately described the
vitamin C losses during both blanching processes.The activation energies and
the reaction rates at 87.5 °C for heat (H) and thermosonication (Ts) treatments
were, respectively, EavitCH = 150.47 ± 42.81 kJ mol- 1 and EavitCTs = 136.20
± 60.97 kJ mol- 1, and k87.5 °CvitCH = 0.75 ± 0.10 min- 1 and k87.5 °CvitCTs
= 0.58 ± 0.11 min- 1. No significant differences (P 0.05) were detected between
both treatments. The thermosonication treatment was found to be a better
blanching process, since it inactivates watercress peroxidase at less severe
blanching conditions and consequently retains vitamin C content at higher
levels. The present findings will help to optimise the blanching conditions for
the production of a new and healthy frozen product, watercress, with heat and
a new blanching process methodology.
In: Innovation Food Science and Emerging Technologies. - New York :
Elsevier. - (2007), 6 p

Biotecnologia / Conservação de alimentos

CDU 66.098

66.098 CRU Efe (ESTGFR) - 144/08

13306. VIEIRA, Maria Margarida Cortês

Role of ODL on sharing pilot plant resources among European Food

Rngineering Universities / M. M. C. Vieira, S. I. Matias, C. Ramalho. - Clique
aqui para acesso ao texto integral :
http://eurodl.org/materials/contrib/2006/Matias_Vieira.htm
In: European Journal of Open, Distance and E-Learning. - [S.L] : [s.n.]. -
(2006), p. 1-13

Biotecnologia / Conservação de alimentos

CDU 66.098

66.098 COR Rol (ESTGFR) - 146/08

13327. MODELAÇÃO DA QUALIDADE MICROBIOLÓGICA DA ÁGUA NA

RIA FORMOSA

Modelação da qualidade microbiológica da água na Ria Formosa / Anabela N.

Venâncio...[et al.]
In: 7º. Congresso da Água. - Lisboa : LNEC, 2004. - 14 p

Qualidade da água / Microbiologia da água / Ria Formosa

CDU 532
CDU 579.63
CDU 628

532 MAR Mod (ESTGFR) - 167/08

13352. EFEITO DO METIONATO DE MANGANÊS NAS PERFORMANCES

DE CRESCIMENTO E ENGORDA E SOBRE A COMPOSIÇÃO DA
CARCAÇA EM SUÍNOS

Efeito do metionato de manganês nas performances de crescimento e engorda

e sobre a composição da carcaça em suínos / J. Rodrigues, J. M. H. du Buf
In: Revista Portuguesa de Zootecnia. - Vila Real : Associação Portuguesa dos
Engenheiros Zootécnicos. - Ano II, nº. 1 (1995), p. 117-128

Suinos

CDU 636.4

636.4 Efe (ESTGFR) - 190/08

13378. CABRITA, Patrícia Alexandra Reis Nunes

Resistance and adaptation to quinidine in Saccharomyces cerevisiae: role of

QDR1 gene (ORF YIL120w), encoding a plasma membrane transporter of the
major facilitator superfamily required for multidrug resistance / Patrícia A.
Nunes, Sandra Tenreiro, Isabel Sá Correia. - Clique aqui para acesso ao texto
integral (restrito à B-on) : http://dx.doi.org/10.1128/AAC.45.5.1528-1534.2001. -
As predicted based on structural considerations, we show results indicating
that the member of the major facilitator superfamily encoded by
Saccharomyces cerevisiae open reading frame YIL120w is a multidrug
resistance determinant. Yil120wp was implicated in yeast resistance to
ketoconazole and quinidine, but not to the stereoisomer quinine; the gene was
thus named QDRI. Qdr1p was proved to alleviate the deleterious effects of
quinidine, revealed by the loss of cell viability following sudden exposure of the
unadapted yeast population to the drug, and to allow the earlier eventual
resumption of exponential growth under quinidine stress, However, QDR1 gene
expression had no detectable effect on the susceptibility of yeast cells
previously adapted to quinidine. Fluorescence microscopy observation of the
distribution of the Qdr1-green fluorescent protein fusion protein in living yeast
cells indicated that Qdr1p is a plasma membrane protein. We also show
experimental evidence indicating that yeast adaptation to growth with quinidine
involves the induction of active expulsion of the drug from preloaded cells,
despite the fact that this antiarrhythmic and antimalarial quinoline ring-
containing drug is not present in the yeast natural environment. However, we
were not able to prove that Qdr1p is directly implicated in this export. Results
clearly suggest that there are other unidentified quinidine resistance
mechanisms that can be used in the absence of QDR1.
In: Antimicrobial Agents and Chemotherapy . - Washington : Amer. Soc.
Microbiology. - Vol. 45, nº. 5 (May 2001), p. 1528-1534

Biotecnologia / Drogas antimaláricas / Leveduras / Parasitologia /

Saccharomyces cerevisiae

CDU 66.098

66.098 CAB Res (ESTGFR) - 217/08

13379. AQR1 GENE (ORF YNL065W) ENCODES A PLASMA MEMBRANE

TRANSPORTER OF MAJOR FACILITATOR SUPERFAMILY THAT
CONFERS RESISTANCE TO SHORT-CHAIN MONOCARBOXYLIC ACIDS
AND QUINIDINE IN SACCHAROMYCES CEREVISIAE

AQR1 gene (ORF YNL065w) encodes a plasma membrane transporter of

major facilitator superfamily that confers resistance to short-chain
monocarboxylic acids and quinidine in Saccharomyces cerevisiae / Sandra
Tenreiro...[et al.]. - Clique aqui para acesso ao texto integral (restrito à B-on) :
http://dx.doi.org/10.1006/bbrc.2002.6703. - We report results on the functional
analysis of Saccharomyces cerevisiae ORF YNL065w, predicted to code for a
protein belonging to the poorly characterized major facilitator superfamily
(MFS) of transporters that are involved in multidrug resistance (MDR).
YNL065w is important for a moderate increase of yeast tolerance to
ketoconazole and to the cationic dye crystal violet; it protects the cell against
short-chain monocarboxylic acids (C2-C6), but not against highly liposoluble
acids such as octanoic acid or the phenoxyacetic-acid herbicides 2,4-D and
MCPA; it is also a determinant of resistance to the antiarrhytmic and
antimalarial drug quinidine. The encoding ORF was, thus, denominated the
AQR1 gene. Results obtained using an AQR1-lacZ fusion indicate that gene
expression is very low and it is not stimulated under weak acid stress. The
encoded putative transporter was localized in the plasma membrane by
fluorescence microscopy observation of the overproduced Aqr1-GFP fusion
protein distribution.
In: Biochemical and Biophysical Research Communications. - New York :
Elsevier. - Vol. 292, nº. 3 (5 April 2002), p. 741-748
 Biotecnologia / Leveduras / Saccharomyces cerevisiae

CDU 66.098

66.098 AQR (ESTGFR) - 218/08

13380. PERKINSUS ATLANTICUS- DESENVOLVIMENTO DE UM MÉTODO

DE DIAGNÓSTICO PARA DETECÇÃO DA INFECÇÃO EM MOLUSCOS
BIVALVES

Perkinsus atlanticus- desenvolvimento de um método de diagnóstico para

detecção da infecção em moluscos bivalves / R. M. Leite...[et al.]. - Clique aqui
para acesso ao texto integral :
http://dequim.ist.utl.pt/bbio/75/pdf/MetDiagMolBivalves.pdf
In: Boletim Biotecnologia. - Porto : Sociedade Portuguesa de Biotecnologia. -
Nº. 75 (Agosto 2003), p. 8-10

Biotecnologia / Parasitologia / Perkinsus / Bivalves / Amêijoas

CDU 66.098
CDU 639.4

66.098 Per (ESTGFR) - 219/08

13381. RESISTÊNCIA A MÚLTIPLAS DROGAS EM SACCHAROMYCES CEREVISIAE: UMA

ABORDAGEM PÓS-GENÓMICA

Resistência a múltiplas drogas em Saccharomyces cerevisiae: uma abordagem pós-genómica / Isabel Sá-
Correia...[et al.]. - Clique aqui para acesso ao texto integral :
http://dequim.ist.utl.pt/bbio/67/pdf/Resistencia%20a%20multiplas%20Drogas%20em%20S.%20cerevisiae.pdf
In: Boletim de Biotecnologia . - Porto : Sociedade Portuguesa de Biotecnologia. - Nº. 67 (Dezembro 2000), p.
8-17

Biotecnologia / Leveduras / Saccharomyces cerevisiae

CDU 66.098

66.098 COR Res (ESTGFR) - 220/08

13382. SHIKIMATE AND FOLATE PATHWAYS IN THE PROTOZOAN

PARASITE, PERKINSUS OLSENI

Shikimate and folate pathways in the protozoan parasite, Perkinsus olseni /

Laurence M. Elandalloussi...[et al.]. - Clique aqui para acesso ao texto integral
(restrito à B-on) : http://dx.doi.org/10.1016/j.molbiopara.2005.03.014. - We
have exploited the experimental accessibility of the protozoan parasite
Perkinsus olseni and its similarities to apicomplexan parasites to investigate
the influence of specific drugs on its proliferation. For this purpose, shikimate
and folate pathways present an attractive target for parasitic therapy given their
major differences with mammalian pathways. Glyphosate, a potent inhibitor of
the shikimate pathway enzyme EPSP synthase inhibited the in vitro
proliferation of P. olseni in a dose-dependent manner and this effect was
reversed by addition of chorismate, indicating the presence of a shikimate
pathway. However, this effect was not antagonised by p-aminobenzoate or folic
acid. Furthermore, antagonism was observed, via pyrimethamine to glyphosate
inhibitory effect, suggesting that the shikimate pathway is not essential for the
biosynthesis of folate precursors and is therefore crucial for another pathway
downstream from chorismate. In addition, sulfadiazine, a well known inhibitor of
dihydropteorate synthase, an enzyme of the folate biosynthetic pathway,had no
inhibitory effect on P. olseni proliferation. In view of these results, the parasite
does not appear to require the folate biosynthesis pathway for its survival and
is most likely able to use exogenous folate. Even though pyrimethamine was
found to inhibit P. atlanticus growth, this inhibitory effect could not be reversed
by co-addition of folic acid. Therefore, we propose that the effect of
pyrimethamine observed in this study results from the inhibition of a target
other than dihydrofolate reductase. Similarly, proguanil target is likely to be
separate from DHFR since only its metabolite cycloguanil has been shown to
have inhibitory properties on DHFR.
In: Molecular and Biochemical Parasitology. - New York : Elsevier. - Vol. 142,
nº. 1 (July 2005), p. 106-109

Biotecnologia / Parasitologia / Perkinsus / Protozoa / Bivalves / Amêijoas

CDU 66.098
CDU 639.4

66.098 Shi (ESTGFR) - 221/08

13383. EFFECT OF ANTIPROTOZOAL DRUGS ON THE PROLIFERATION

OF THE BIVALVE PARASITE PERKINSUS OLSENI

Effect of antiprotozoal drugs on the proliferation of the bivalve parasite

Perkinsus olseni / Laurence M. Elandalloussi...[et al.]. - Clique aqui para
acesso ao texto integral (restrito à B-on) :
http://dx.doi.org/10.1016/j.aquaculture.2004.09.038. - The protozoan parasite
Perkinsus olseni causes severe losses among Ruditapes decussatus clams.
The development of an in vitro culture of this parasite together with the use of a
proliferation assay has provided the opportunity to screen for drug sensitivity of
this parasite. Xenobiotics known for their antimalarial and antiprotozoal
properties were tested against P. olseni. Only four of these drugs, namely
cycloheximide, pyrimethamine, deferoxamine (DFO) and 2,2-bipyridyl (BIP),
showed in vitro inhibitory effect on the parasite proliferation. Two in vivo
experiments were designed to determine the effect of iron chelators on
reducing P. olseni infection in clams. For this purpose, naturally infected clams
from the Ria Formosa, Portugal, were exposed to DFO and BIP at various
concentrations. In the first experiment, hemolymph samples were taken from
each clam before and after treatment to determine the infection intensity and in
the second experiment, clams were randomly distributed in groups of five and
the parasite burden in treated and untreated groups was determined at the end
of the experiment on the whole clam wet tissues. Only DFO was found to be
effective in reducing in vivo P. olseni infections. In addition, acute toxicity of
DFO and BIP has been determined and no mortality of Perkinsus-free clams
was observed.
In: Aquaculture. - New York : Elsevier. - Vol. 243, nº. 1-4 (January 2005), p. 9-
17

Biotecnologia / Parasitologia / Perkinsus / Doença de protozoário / Molusco /

Bivalves / Amêijoas
CDU 66.098
CDU 639.4

66.098 Eff (ESTGFR) - 222/08

13384. DEVELOPMENT OF A PCR-ELISA ASSAY FOR DIAGNOSIS OF

PERKINSUS MARINUS AND PERKINSUS ATLANTICUS INFECTIONS IN
BIVALVE MOLLUSCS

Development of a PCR-ELISA assay for diagnosis of Perkinsus marinus and

Perkinsus atlanticus infections in bivalve molluscs / Laurence M.
Elandalloussi...[et al.]. - Clique aqui para acesso ao texto integral (restrito à B-
on) : http://dx.doi.org/10.1016/j.mcp.2003.09.008 . - Perkinsus atlanticus and P.
marinus have been associated with mass mortality of bivalve molluscs.
Perkinsus infections are routinely diagnosed by histology or the fluid
thioglycollate medium (FTM) assay. In this study, we describe the development
of a PCR-enzyme-linked immunosorbent assay (ELISA) for amplification and
rapid detection of Perkinsus species. The PCR reactions were selected to
either amplify an IGS sequence region shared by currently accepted Perkinsus
species or to simultaneously amplify IGS regions specific to either P. atlanticus
or P. marinus. The specific hybridisation of DIG-labelled amplified products to
species-specific capture probes was detected colorimetrically. This assay is
able to specifically detect P. atlanticus and P. marinus, and the intensity of the
colorimetric signal is dependent upon the amount of amplified product. The
PCR-ELISA assay format is 100-fold more sensitive than visualisation of PCR
products on ethidium bromide (EtdBr)-stained agarose gels, and as sensitive
as Southern hybridisation. The sensitivity limit of PCR-ELISA was 1 pg of DNA
from P. atlanticus. No cross-reactivity of the assay was observed against the
host DNA. When applied to the detection of P. atlanticus in clams, 39 samples
out of 45 yielded concordant results for FTM assay and PCR-ELISA detection.
In: Molecular and Cellular Probes . - New York : Elsevier. - Vol. 18, nº. 2 (April
2004), p. 89-96

Biotecnologia / Parasitologia / Perkinsus / ELISA / Bivalves / Amêijoas

CDU 66.098
CDU 639.4

66.098 Dev (ESTGFR) - 223/08

13385. ARE SARDINE LARVAE CAUGHT OFF NORTHERN PORTUGAL IN

WINTER STARVING? AN APPROACH EXAMINING NUTRITIONAL
CONDITIONS

Are sardine larvae caught off northern Portugal in winter starving? An approach
examining nutritional conditions / Eduardo Esteves...[et al.]. - Clique aqui para
acesso ao texto integral(restrito à B-on) : http://www.int-
res.com/articles/meps2003/257/m257p303.pdf. - Recently, winter upwelling
events off western Iberia have become more frequent. This may affect the
production and survival of sardines egss and larvae through increased offshore
transport. By analysis of RNA: DNA ratios, we investigated the impact of winter
upwelling events on the larval conditions of Sardina pilchardus larvaeas a
function of oceanographic conditions and food availability. Larvae were
collected on a research cruise off northern Portugal in February 2000.
Environmental parameters such as wind, water temperature, salinity
 microzooplankton biomass and daily egg production of the calanoid copepod
Calanus helgolandicus also measured. The mean RNA:DNA ratios
wererelatively high, indicating that almost all larvae collected were good
condition. This was in agreement with the high microzooplankton biomass and
high daily egg production of the copepod C. helgolandicus recorded during the
same period. No adverse effects of upwelling causing offshore transport of
larvae into poor feeding areas could be demonstrated because of the presence
of a stratified warm plume with consequent high food production.
In: Marine Ecology Progress Series. - Germany, 2003. - v. 257; 2003, p. 303 -
309

Peixe / Sardinha

CDU 664.95

664.95 ARE (ESTGFR) - 224/08

13388. THE DISTRIBUTION OF ESTUARINE FISH LARVAE : NUTRITIONAL

CONDITION AND CO-OCCURRENCE WITH PREDATORS AND PREY

The distribution of estuarine fish larvae : Nutritional condition and co-

occurrence with predators and prey / Eduardo Esteves...[et al.]. - Clique aqui
para acesso ao texto integral (restrito à B-on) :
http://dx.doi.org/10.1016/S1146-609X(00)01078-X. - Fish larvae were collected
monthly between March and September 1997 in the Mira and Guadiana
estuaries (southern Portugal). Hydrological parameters were registered and
zooplankton samples were obtained simultaneously. Densities of fish larvae
(ind.100 m-3) were calculated from 211 samples and larval nutritional condition
measured as RNA/DNA ratios were obtained for 346 individuals, using a
fluorimetric method for nucleic acid quantification. Correlating variables were
further studied using multiple regression analysis in order to assess the relative
importance of abiotic trends in abundance and nutritional condition of estuarine
fish larvae. Results indicated that: 1) the abundance of fish larvae seems
conditioned by temperature and predation; and 2) their nutritional condition is
dependent on temperature and prey availability. Temperature is an important
variable structuring estuaries and therefore conditions the behaviour and
physiology of fish larvae. Furthermore, the co-occurrence of predators and
larvae might be related to similar feeding patterns or comensalism Whenever
feeding conditions are suitable, they usually determine enhanced growth and
nutritional condition. However, predation seems to control this latter
relationship through its effect on larval mortality.
In: Acta Oecologica,. - Paris : Elsevier. - Volume 21, Number 3, 6 May 2000 ,
p.161-173

Fish larvae / distribution / RNA/DNA ratios / Nutrition condition

CDU 664.95

664.95 (ESTGFR) - 227/08

13389. GROSS AND HISTOLOGICAL OBSERVATIONS OF OVARIAN DEVELOPMENT IN

TWAITE SHAD, ALOSA FALLAX FALLAX, FROM RIVERS MIRA AND GUADIANA
(PORTUGAL)

Gross and histological observations of ovarian development in twaite shad, Alosa fallax fallax, from
Rivers Mira and Guadiana (Portugal) / Eduardo Esteves, Teresa Pina, José Pedro Andrade. -
Clique aqui para acesso ao texto integral em formato PDF :
http://www.icm.csic.es/scimar/download.php/Cd/84d79b133c5cdc2e3455cc97c741c096/IdArt/3062.
- In order to describe the stages of oogenesis of twaite shad, Alosa fallax fallax, 265 females were
collected between March and June 1997, February and June 1998 and January and April 1999 in
the Rivers Mira and Guadiana. From the histological study of ovaries a total of eight developmental
stages were delineated. Gross examination of paired ovary revealed that they could be placed into
one of seven maturity stages according to their stage of development. Two stages of atresia, alfa
and beta,were identified. Upon cessation of spawning, the ovaries still contained some oocytes at
various stages of development but with a greater number of atretic oocytes. The simultaneous
occurrence of tes at different stages of development in the ovary indicates asynchronous oocyte
development. Oocyte size frequency distributions do not show a gap in size between cortical alveoli
and vitellogenic oocytes during the spawning season. This may represent the ability of twaite shad
to push oocytes through vitellogenesis from a previtellogenic condition during the spawning period.
This has important implications for twaite shad fecundity, because fish with this type of oocyte
development depend on timates of batch fecundity and spawning frequency to determine potential
annual fecundity.
In: Scientia Marina. - vol. 67(3) 2003, p. 313-322

oogenesis / oocyte dynamics

CDU 664.95

664.95 (ESTGFR) - 228/08