Beruflich Dokumente
Kultur Dokumente
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R
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The microorganisms and their products are closely related to the
etiology of pulpal and periapical lesions. They can cause pulp
necrosis due to their persistence in the root canal system after
endodontic treatment and can induce a periapical inflammatory
reaction.
The presence of R in the infected pulp and
periradicular areas has been demonstrated through the use of light
and electron microscopy, as well as using culture techniques. The
incidence of R in infected root canals has been shown to
vary between 7% and 55% and occurs in approximately 11.36% of
teeth with pulp lesions. It has also been reported that R can
use dentin as a nutrient source.
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1.c Determine the number colony presence of the antifungal
agent.
2.c Determine the number colony presence of the antifungal
and 2% chlorhexidine.
3.c Compare the two methods.
4.c Which method is most effective in reduce the number of
colony.
5.c Which method is least effective in reduce the number of
colony.
R
1. Antifungal efficacy of 5.25% sodium hypochlorite, 2%
chlorhexidine Gluconate, and 17% EDTA with and without an
antifungal agent.
2. Antifungal effects of sodium hypochlorite and chlorhexidine in
root canals.
3. Evaluation of the interaction between sodium hypochlorite and
chlorhexidine gluconate and its effect on root dentin.
4. Antifungal efficacy of 5.25% sodium hypochlorite, 2%
chlorhexidine gluconate, and 17% EDTA with and without an
antifungal agent.
5. Antifungal effects of sodium hypochlorite and chlorhexidine in
root canals.
6. Evaluation of the interaction between sodium hypochlorite and
chlorhexidine gluconate and its effect on root dentin.
7. Comparison of the antibacterial efficiency of neem leaf extract and
2% sodium hypochlorite against e. faecalis, c. albicans and mixed
culture - an in vitro study.
8. Effectiveness of a 2% chlorhexidine solution mixed with calcium
hydroxide against candida albicans effectiveness of a 2%
chlorhexidine solution mixed with calcium hydroxide against
candida albicans.
9. In vitro study of the effectiveness of intracanal irrigants on
candida albicans.
10.Antimicrobial action of calcium hydroxide, chlorhexidine and
their combination on endodontic pathogens.
11. Antimicrobial activity of sodium hypochlorite associated with
intracanal medication for
inoculated in root canals.
12. Antimicrobial activity of varying concentrations of sodium
hypochlorite on the endodontic microorganismǯsantinomiesIsraeli,
a. naeslundii, candida albicans and enterococcus faecalis.
13.Clinical aspects related to endodontic yeast infections.
14. Comparison of the antibacterial efficiency of neem leaf extract
and 2% sodium hypochlorite against e. faecalis, c. albicans and
mixed culture - an in vitro study.
15. Effect of sodium hypochlorite and five intracanal medications on
in root canals
16.Effectiveness of a 2% chlorhexidine solution mixed with
calciumhydroxide against candida albicans
17.Evaluation of antifungal efficacy of 5% doxycycline
Hydrochloride, 2.5% sodium hypochlorite, 17%ethylenediamine
tetraacetic acid and 0.2% chlorhexidinegluconate against candida
albicans - an in vitro study.
18. In vitro evaluation of the antimicrobial activity of
chlorhexidineand sodium hypochlorite
19.In vitro study of the effectiveness ofintracanal irrigants on
candidaalbicans
20.Antifungal effect of zeolite-incorporated tissue conditioner
against Candida albicans growth and/or acid production.
R
The anti-fungal effect of 2% chlorhexidine solution,Antifungal agent
(° ) and 2% chlorhexidine mixed other agent is 2%
chlorhexidine solution,calcium hydroxide paste with a calcium
hydroxide was evaluated against C. albicans.
Stock cultures of clinical isolates of C. albicans were provided by the
Microbiology Laboratory of university of Philippines manila and
maintained in Sabouraudǯs dextrose agar plates.
A suspension was prepared by transferring three colonies from a
Sabouraudǯs dextrose agar plate, using a sterile 4 mm diameter
platinum loop, to a 10 mm Sabouraud infusion broth in a sterilised
10 mL screw capped test tube, followed by incubation for 1 week
at37oC. two such test tubes were prepared.
The experiments were performed in plastic tissue cultureclusters
containing 24 wells, each with an inner diameterof 16 mm. One mL
of test material was placed at thebottom of each culture well, to
which 1 mL of a R suspension was added. At the same time, 1
mL of Sabouraudinfusion broth media was mixed with 1 mL of
aR suspension in a culture well to serve as a positivecontrol.
For the negative control, 2 mL of Sabouraudinfusion broth was
placed in a culture well. Six wells werethus used per test. The
culture clusters plates were thenincubated at 37oC and evaluated
after 1, 24 and 72 h.
At the end of the incubation period, aliquots of 0.1 mL
Were withdrawn from each well and transferred to tubes containing
5 mL of fresh Sabouraud infusion broth. The tubes were then
vortexed, incubated at 37oC, and observed for 7 days. Growth of the
fungi was observed daily, as indicated by the presence of turbidity in
the tubes. The presence of turbidity was determined, and the purity
of the cultures was checked by the morphology of colonies onto
blood agar plates. The results were statistically analyzed using the
KruskalȂWallis test.
Solution
1.cControl solution
2.c2% chlorhexidine mixed with Antifungal agent (° )
3.c2% chlorhexidine mixed with calcium hydroxide paste
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