Information Brochure

INFORMATION BROCHURE
Industrial Training Certificate Program in “State of the Art Lectures

and Hands on Training in Biotechnology, Molecular Biology, Pharma,
QC & Bioinformatics”
The Training Director, Dr Rai Ajit Srivastava, Ph.D., FAHA, MBA, is a Visiting Prof. at Univ Palermo, Italy
& Wayne State Univ, USA. He served as Associate Prof at the Washington Univ School of Medicine, St Louis,
and Head of Preclinical Research at Tularik Pharmacy Co & Bristol-Myers Squibb, USA, and Executive V P at
Piramal Life Sciences, Mumbai, and currently working as Executive Director, Esperion Therapeutics Inc,
USA
From The Training Director’s Pen

The field of science has been developing at a rapid pace, and at times it becomes extremely impractical to
capture all information that becomes available through research journals and online sources. This course
was developed with a view to encompass rapid changes in science. The course materials are, therefore,
updated every 6 months to add new information. The course is designed such that it covers most of the
advanced laboratory techniques to study and analyze proteins/enzymes, DNA, RNA and apply recombinant
DNA technology for specific needs. In addition, this course also consists of some of the most advanced
technologies like transgenesis, gene knockout, RNAi, SAGE, microarray, real-time PCR and monoclonal
antibody production. On of the aims of this course is to train students in drug discovery and development,
not by discussing laboratory data generated in the course-developer’s labs. This course is unique in terms of
providing all information related to biotechnology and drug discovery at one place. Early discovery science to
understand how the disease is caused at a molecular level together with clues in both the patients’
experience of the disease and the compendium of medical knowledge that humans have assembled for
centuries compiled with the growing knowledge of human biology and genetics, are discussed. The best
possible experimental designs to test ypotheses and yield answers to unanswered questions about how the
disease works and how it can be treated also are part of the course materials.
Why do we need such a course at this time? The global pharmaceutical market is estimated at US$ 427
billion and R & D cost is estimated at US$ 60-65 billion annually. R & D Industry has opened up new vistas
of employment for a large number of people. India has become one of the best destinations for
Biotechnology industry due to good network of research laboratories, rich biodiversity, establishment of
several state-of-the-art CRO’s with GLP facilities, well developed base industries, rich agriculture sector and
trained manpower that speaks English fluently. While pharmaceutical industries in the USA, and to some
extent in the Europe, are going through tough time resulting elimination of thousands of research jobs due
to mergers and acquisitions, India has seen strong growth in this sector for the past few years, and job
opportunities in R & D sector have increased. In addition, India also offers tax concessions on revenue to
companies making R&D investments in India. These incentives are expected to substantially increase R&D
activities of both multinational and domestic biopharmaceutical companies. An added advantage is that
India's pharmaceutical market is the second largest in Asia, growing by more than 9% annually.
We believe that at this time training students in hands-on techniques and providing relevant knowledge to
become successful in biotech industries are extremely important. This course contains 22 lectures by world
fame scientists of the same field. These lectures describe actual experiments and basic science as well as
some laboratory exercises that cover current principles and techniques used in molecular biology and
biotechnology. The main objective of the training is to develop critical thinking, design experiments,
interpret research data, develop presentation skills, gain hands-on experience on advanced techniques and
methodology of Biotechnology and Molecular Biology. All students have access to Institute's computer and
Internet facilities, enabling them in data searches. We provide state of the art infrastructural amenities at
our center of excellence. It is a perfect environment conducive to effective learning for academic success. A
state of the art infrastructure having high speed servers and round the clock internet connectivity is
available to students for the purpose of learning. I wish all trainees the best of learning and excelling in their
chosen area.
@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 1

TRAINING DETAILS
LECTURES: (PART - 1)
Lecture-1 Working in Biotech Lab

Lecture-2 Structure and Function of DNA and RNA
Lecture-3 Recombinant DNA Technology-1
Lecture -4 RECOMBINANT DNA TECHNOLOGY -2
Lecture-5 Library construction-DNA analysis
Lecture-6 Gene regulation and RNA Analysis
Lecture-7 Protein Structure
Lecture-8 Protein and Enzyme Analysis
Lecture-9 Protein Analysis -antibody production
Lecture-10 Protein Purification1
Lecture-11 Protein Purification2
Lecture 12 Knockout technology
Lecture 13 KO Cell-Transgenesis
Lecture 14 Tissue Culture 1
Lecture 15 Tissue Culture 2
Lecture 16 Functional Genomics
Lecture 17 Physical Genomics
Lecture 18 RNAi and Taqman
Lecture 19 Drug Discovery 1
Lecture 22 Job Interview Tips
Presentation of Trainees Research Findings to the Group
Review of Research Paper as Presentation to the Group
CORPORATE FACULTY & CONSULTANTS:
Dr. Ajit Srivastava Executive Director at Esperion Therapeutics, USA
Dr. Neelam Srivastava Research Scientists, Bristol-Myers Squibb, NJ, USA
Dr. Salman Azhar Director and Professor, Stanford University, Palo Alto, CA, USA
Dr. Anand Swaroop Professor, Dept of Genetics, University of Michigan, Ann Arbor, USA
Dr.T.B. Rajavashishtha Professor, UCLA, CA, USA
Mr. Ravi Jahagirdar Director, Pharmacology, Resverlogix, Canada
Prof Debomoy K Lahiri Inidana University School of Medicine, Indianapolis, USA
Dr Rangarajan Narayanan Former Research Scientist at Bristol-Myers Squibb, USA
Prof Khosrow Adeli Professor, University of Toronto, Canada
Prof Mahendra Thakur Professor of Zoology, Banaras Hindu University, Varanasi
Prof Maurizio Averna Professor of Medicine, Palermo University, Italy
Dr. Vandana Tiwari Sr. Research Scientist, CloneGen Biotechnology Pvt. Ltd., NOIDA
NOTE:
1. For more details of Lecture’s Sub Headings, send your request to – module@clonegenbiotech.com
2. Research Paper & Study materials will be provided to trainees for each lecture.

HANDS ON MODERN TECHNIQUES IN LABORATORY: (PART -2)
SECTION -1A: MICROBIOLOGY
Sr. No. Content

1 Instrumentation
2 Media preparation for Bacterial Cultures.
3 Serial Dilution Method for Bacterial Cultures.
4 Pouring, Plugging, Slant preparation & Streaking on plates.
5 Sub Culturing of Microbes
6 Isolation and Culturing of microbes from Soil Sample.(Through Serial
Dilution Method)
7 Isolation and Culturing of microbes from Water Sample.(Through Serial
Dilution Method)
8 Isolation and Culturing of microbes from Air. (Through Exposure Method).
9 Biochemical Test For Screening of Microbes
10 Growth Kinetics of Microbes
11 Antibiotics Sensitivity Test
12 LB-media preparation, inoculation and culturing of E. coli.
SECTION -1B: MICROBIOLOGY(Newly Introduced)
Sr. No. Content

1 Endospore staining
2 Capsular staining
3 Flagellar staining
4 Fungal staining
5 Actinomycetes staining
6 Isolation and identification of rhizobia from root nodules
7 Isolation and identification of enzyme producing microbe (amylase)
8 Isolation of antibiotic producing microbe by crowed plate method
9 Isolation of antibiotic resistance microbe from soil
10 Fungal staining with lactophenol blue
11 Gram Staining for Bacterial Sample

SECTION -2: MOLECULAR BIOLOGY
Sr. No. Content
1 Plasmid isolation
2 DNA isolation from microbes.
3 DNA isolation from plant sample.
4 Agarose Gel Electrophoresis.
5 RNA isolation
6 RNA Gel Electrophoresis.
7 Qualitative & Quantitative analysis of Nucleic Acid
8 Protein Isolation
9 SDS-PAGE
10 Southern Blotting
11 Northern Blotting
12 Western Blotting
13 DOT ELISA
14 Competent Cell Preparation.
15 Transformation Through Blue-White selection.
16 Polymerase Chain Reaction (PCR).
SECTION -3A: BIOCHEMISTRY

Sr. No. Content
1 Estimation of Protein by Bradford Method.
2 Estimation of Protein by Biurette Method.
3 Estimation of Protein by Lowry’s Method.
4 Enzyme Assay – Amylase Assay ,SGPT, SGOT, ALP
SECTION -3B: BIOCHEMISTRY(Newly Introduced)

Sr. No. Content
1 Sauerkraut Production(Fermented food) - solid state fermentation
2 Beta-galactosidase enzyme assay
SECTION -4: CHROMATOGRAPHY TECHNIQUES

Sr. No. Content
1 Paper Chromatography
2 Thin Layer Chromatography
3 Column Chromatography
4 HPLC

PART :3= PHARMA, QUALITY CONTROL & MICROBIOLOGY
Sr. No. Content

PRODUCTION - TABLETS
1 Sampling
2 Dispensing
3 Mixing – Granulation – Drying
4 Tabletting
5 In process – Controls – Product – Environment
6 Packing – Blister
7 Beta-galactosidase enzyme assay
8 Ames test
QUALITY CONTROL
1 Weighing – Calibration of Balance
2 Preparation of Solutions/ Titration
3 Melting Point
4 Heavy Metals
5 Loss on Drying
6 UV
7 HPLC
MICROBIOLOGY
1 Instrumentation
2 Media preparation for Bacterial Cultures.
3 Serial Dilution Method for Bacterial Cultures.
4 Pouring, Plugging, Slant preparation & Streaking on plates.
5 Sub Culturing of Microbes
6 Isolation and Culturing of microbes from Soil Sample.(Through Serial
Dilution Method)
7 Isolation and Culturing of microbes from Water Sample.(Through Serial
Dilution Method)
8 Isolation and Culturing of microbes from Air. (Through Exposure Method)
9 Biochemical Test For Screening of Microbes
10 Growth Kinetics of Microbes
11 Staining Techniques for Bacterial Sample
12 Antibiotics Sensitivity Test
13 LB-media preparation, inoculation and culturing of E. coli.

TRAINING OPTIONS
1. Certificate Program in “Hands on Industrial Training in Advanced Biotechnology and

Molecular Biology" (CODE – CER-01) (Techniques without Project Work)
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A, 2 & 3A of Part-2 will be
covered)
B Lectures On Lab Techniques
DURATION - One Month

SEAT - 15
FEE STRUCTURE - 6,500/- (Service Tax Extra)
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A&B, 2 & 3 A&B of Part-2
will be covered)
DURATION - One & Half Month

SEAT - 15
FEBRUARY BATCH-2011
LAST DATE FOR REGISTRATION -Till February 03, 2011
STARTING DATE - February 04, 2011
MARCH BATCH-2011
LAST DATE FOR REGISTRATION -Till March 02, 2011
STARTING DATE - March 03, 2011
APRIL BATCH-2011
LAST DATE FOR REGISTRATION -Till April 04, 2011
STARTING DATE - April 05, 2011
2. Certificate Program in “Hands on Industrial Training in Advanced Biotechnology

and Molecular Biology" (CODE – CER-02) (Techniques and Project Work)
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A,2 & 3A of Part-2 will be
covered)
C Hands-on Project Work
DURATION - Two Months

SEAT - 15
TOPICS:
will be covered)
DURATION - Two & Half Months

SEAT - 15
FEBRUARY BATCH-2011
MARCH BATCH-2011
APRIL BATCH-2011

TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A,2 & 3A of Part-2 will be
covered)
DURATION - Three Months

SEAT - 15
TOPICS:
will be covered)
DURATION - Three & Half Months

SEAT - 15
FEBRUARY BATCH-2011
MARCH BATCH-2011
APRIL BATCH-2011

TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A,2,3A & 4 of Part-2 will
be covered)
DURATION - Three & Half Months

SEAT -15
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A&B,2,3A&B & 4 of Part-2
will be covered)
DURATION - Four Months

SEAT -15
FEBRUARY BATCH-2011
MARCH BATCH-2011
APRIL BATCH-2011

and Molecular Biology" (CODE – CER-05) (Techniques without Project Work)
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 2A,3A & 4 of Part-2 will be
covered)

SEAT -15
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 2,3A&B & 4 of Part-2 will be
covered)

SEAT -15
FEBRUARY BATCH-2011
MARCH BATCH-2011
APRIL BATCH-2011

and Molecular Biology" (CODE – CER-06) (Techniques without Project Work)
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A,3A & 4 of Part-2 will be
covered)

SEAT - 15
TOPICS:
A Hands-on Modern Techniques In Lab (Section – 1A&B,3A&B & 4 of Part-2
will be covered)

SEAT - 15
FEBRUARY BATCH-2011
MARCH BATCH-2011
APRIL BATCH-2011

7. Certificate Program in "Hands-on Industrial Training and State-of-the-art
Lectures in Advanced Biotechnology and Molecular Biology" (CODE – CER-07).
“NGO SPONSORED PLACEMENT ORIENTED TRAINING
PROGRAM”*
TOPICS:
A State-of-the-art Lectures in Advanced Biotechnology and Molecular Biology (Part-1)
B Hands-on Modern Techniques In Lab (Section – 1A,2,3A & 4 of Part-2 will
be covered)
C Lectures On Lab Techniques & Project
D Hands-on Project Work
DURATION – Four months

SEAT - 20
BATCH-2011
BATCH STARTING DATE - 3 MARCH 2011
LAST DATE OF REGISTRATION - 09 FEBRUARY 2011
ONLINE EXAM DATE - 10 FEBRUARY 2011
RESULT DECLARATION - 11 FEBRUARY 2011
NOTE:
1. For Getting Complete module details, please send your request to –
module@clonegenbiotech.com
2. Registration of the candidate would be valid for next three months from the
date of registration. (If candidate is unable to join the current batch.
3. Reservation of the seat by the candidate would be valid for the next six months
from the date of the reservation. (If the candidate is unable to join the current
batch.)
4. Online Entrance Examination Fee – Rs. 550/-.
*For Getting Details of NGO (S-LEARN) Sponsorship , please send your request to –
cer07@clonegenbiotech.com

Lectures in Pharmaceutical & Quality Control" (CODE – CER-08). *
TOPICS:
A Hands-on Modern Experiments In Lab (Part -3 will be covered)
DURATION – Two Months

SEAT - 20
FEBRUARY BATCH-2011
* In the collaboration of Cotec Pharma, Ghaziabad (http://www.cotecpharma.com)

BIOINFORMATICS TRAINING MODULE
Lectures in Bioinformatics" (CODE – CER-09).
OPTION A
 Introduction  Databases & Tools  Sequence Alignment
 Structure Alignment  Software Tools & their  Drug Discovery
Applications
DURATION – One Month

SEAT - 10
OPTION B
 Structure Alignment  Applications  Drug Discovery
 Modelling
DURATION – Two Months

SEAT - 10
OPTION C
 Structure Prediction  Modelling  Phylogenetic Analysis
 Microarrays  Drug Discovery & Drug  PROJECT WORK
Design
DURATION – Three Months

SEAT - 10
OPTION D
 Microarrays  Drug Discovery & Drug  PROJECT WORK
Design
DURATION – Four Months

SEAT - 10
FEE STRUCTURE - 15,000/- ( Service Tax Extra)
OPTION E
 Microarrays  Drug Discovery & Drug  Proteomics
Design
 Genomics  Pharmacogenomics  PROJECT WORK
DURATION – Six Months

SEAT - 10
FEBRUARY BATCH-2011

DETAIL TRAINING MODULE OF BIOINFORMATICS (CER-09)
1. INTRODUCTION: 2. APPLICATIONS:
(a) Basic Concept, NCBI, ENTREZ, EXPASY, SWISS PROT (a) Immuno informatics
(b) History (b) Geno informatics
(c) Proteins, DNA RNA Structure & Functions- PDB (c) Chemo informatics
(d) Human Genomics Project (d) Medical & Clinical Informatics
(e) Protein Engineering (e) Argo Informatics
(f) Drug Design (f) Forensic Informatics
Software to learn:- NCBI database, Entrez database, Expasy (g) Bioinformatics in Ayurvedics
database , SWISS PROT , Protein data bank and tools involved.
3. BIOINFORMATICS DATABASE & TOOLS: 4 SEQUENCE ALIGNMENT:
(a) Types of Database (a) Introduction

(b) Nucleotide sequence databases (10 & 20 database) eg.: (b) Global & Local Alignment
EMBL, Genebank, DDBJ, UNIGEN, SGD, dbest, Kabat, IMG (c) Pair wise Sequence Alignment
(c) protein Sequence Database - BLAST (BLAST family)
- PDB - FASTA
- EMBOSS
- SWISS PROT
(d) Multiple sequence alignment
- PIR
- CLUSTAL W
- SCOP - T – COFFEE
- CATH - MAFFT
- Kabat - MUSCLE
- TrEMBL - EMBOSS GUI
(d) Sequence motifs (e) Scoring Matrices
- PROSITE - BLOSSUM
- Pfam - PAM 30/250
(e) Other Important database
- KEGG
- PQS
(f) Indian Database
- Vanshanudhan
- GM Corp database
SOFTWARES TO LEARN:- PROTEIN VISUALIZATION TOOLS :-
RASWIN, CHIMERA, SPDBV, PYMOL, JMOL, LASERGENE DNA STAR,
CTL PRED, EXPASY TOOLS, PROTMARAM, PROTMUTATION
5. STRUCTURAL ALIGNMENT: 6 STRUCTURE PREDICTION
(a) Protein Structure alignment (a) Introduction

(b) Introduction (b) Lower proteins structure predictions
(c) Different alogrithms - PSIPRED
- PHD
- DAL
- SAM
- VAST
- Tm – align
7 MODELING: 8 INFORMATION SEARCH AND ITS

(a) Homology modeling APPLICATIONS:
(b) Comparative Modeling
- SwissModeller (a) Phylogenetic Analysis
(c) Ab initio modeling - PHYLIP
- UNRES (b) Microarrays
- TOUCHSTONES - Array Axpress
(d) Combined modeling approaches (c) Sequence assembly
SOFTWARES TO LEARN:- SWISS-MODEL ,3Djigsaw ,CPHmodels , - POLYPHRED
ESyPred3D, Geno3d ,Phyre, HMMSTR/Rosetta, MODELLER (d) Proteomics
PROCHECK, WHAT IF, PROSA II, VERIFY 3D, Profile3D (e) Genomics
(f) Pharmacogenomics

9 BIOINFORMATIC DRUG DISCOVERY:
(a) Introduction
- Overview
- CADD & QSAR
- Insilico DD
- High through put DD
- Genomics DD
- Proteomics DD
- Target validation
(b) Brief
- Structural Bioinformatics in Drug Discovery
- Drug Designing Process
- Drug Discovery Softwares
- PSIPRED
- RCSB (PDB)
- Principle of Drug Action
SOFTWARES TO LEARN:-
FOR DOCKING: - HEX, Z-DOCK, DOCKING SERVER,
FOR STRUCTURE: CHEMSKETCH, PUBCHEM, CAST P
CER – 10
GOLDEN OPPORTUNITY TO ENHANCE YOUR PRACTICAL
SKILLS
Hands-On Workshop on weekends on latest Biotechnology
techniques
ORGANISED BY
Clonegen Biotechnology Pvt. Ltd., NOIDA
Jointly with
Clonegen Biotechnology, LLC, USA
You can Choose and Attend from following group:-
GROUP 1:- (3 Days Module) GROUP 2:- (3 Days Module)
1) DNA Isolation 1) DNA Isolation
2) Plasmid Isolation 2) PCR
3) Agarose gel electrophoresis 3) Southern Blotting
4) Southern Blotting
GROUP 3:- (3 Days Module) GROUP 4:- (3 Days Module)
1) SDS-PAGE 1) RNA Isolation
2) Western Blotting 2) RNA gel electrophoresis
3) Southern Blotting 3) Northern Blotting
4) Northern Blotting

GROUP 5:- (3 Days Module) *GROUP 6:- (one week module)
1) Paper chromatography 1) DNA Isolation
2) Thin layer chromatography 2) Agarose gel Isolation
3) Column chromatography 3) PCR
4) Southern Blotting
5) RNA Isolation
6) RNA gel electrophoresis
7) Northern Blotting
8) SDS-PAGE
9) Western Blotting
10) Chromatography (Paper,
thin layer, column, etc.)
Eligibility Criteria:
The candidates of B.Sc., B.Tech., B. Pharma, M. Pharma. M.Sc., M.Tech. Biotechnology/Molecular
Biology/ Microbiology/Biochemistry/Bioinformatics/Other Life Sciences either Pursuing or
passed out can apply.
Certification:
Those who will successfully complete the Workshop will be given Industrial Workshop certificate.
Registration Process:
SEE NEXT PAGE
Participation Fee: ( Service Tax Included ,Registration fee Excluded)
GROUP 1:- (3 Days Module) Rs. 1500/-

*GROUP 6:- (one week module) Rs. 4500/-
Workshop Dates:
Workshop Date Last Date of Registration

JANUARY 2011 MONTH
20,21,22 19 January 2011
27,28,29 25 January 2011
NOTE:
1. SEND YOUR ANY QUERY TO - workshop@clonegenbiotech.com

2. Study Material and Protocol for experiments will be provided to each participant free of
Cost.

HOW TO APPLY
OPTION 1: THROUGH WEBSITE
► Before Starting Registration through our website, firstly you should have DD of Rs.
550/- in the favour of "Clonegen Biotechnology Pvt. Ltd." payable at
NOIDA/DELHI.
► Register yourself by visiting our Website – http://www.clonegenbiotech.com under
trainee Registration.
► After Registration, you will get USER ID and Password on your registered mail ID
to Login.
► Use your USER ID & Password and Login to your account to get Complete
Acknowledgement of Registration. Take the Print out of Acknowledgement Letter.
► Send the Acknowledgement Letter and DD to our Company Address.
► After your registration through our website, instantly your Seat for Concern Batch
and Program will be Reserved even we receive your DD & Acknowledgement Letter
later on.
OPTION 2: BY DOWNLOADING APPLICATION FORM
► Download the Application Form through our Website.

► Fill it completely as per instruction given in Form.
► Send Completed Form along with your updated CV and DD of required amount to
our Company Address through Courier only.
► Only after receiving your complete form by our office, your Seat for Concern Batch
and Program will be Reserved.
OPTION 3: BY VISITING COMPANY
►Download Application Form from our website.

►Fill Application Form and submit with Rs. 550/- Cash to the office.
NOTE - For Any Further Information, please contact to company address or
send your query to - query@clonegenbiotech.com

SERVICES PROVIDED BY CLONEGEN BIOTECH TO SELECTED TRAINEES
FOR INDUSTRIAL TRAINING
1) Trainee will get 2 separate
books developed by Corporate
Scientists from USA and India.
Because of the rapid
advancements in science, Books
are revised at least twice a year,
so that the trainees obtain up-to-
date knowledge and
experience.(Only For CER-07)
CBPL BUILDING
2) Trainee will get Lab Protocol
Booklet to learn and revise different
Molecular Biology/
Biochemical/Microbiological
Techniques.
CBPL RECEPTION
3) CloneGen Premises is WI – FI
Enabled. Trainee will get Internet
Access to search and study different
research papers as per their
requirement round the clock.
LECTURE HALL

4) Trainee will get all the Chemicals &
Reagents to carry out their
Techniques and Project work.
TRAINING LAB
5) Trainee will get personalized
attention by Lab Trainers and will
individually get Hands – on – training
for each of the Techniques.
TRAINING LAB
6) Trainee will get Library Facility
without any extra charges.
TRAINING LAB
7) Trainee will get Research Papers
related to every lecture to understand
the process of Research work.
TRAINING LAB

8) Trainee will get ISD Call facility to
US & Canada, to discuss their
queries/problems to our Corporate
Scientists and Scientific Consultants.
TRAINING LAB
9) For outstation candidates, Clonegen
will assist them to find out right
Accommodation very nearby to
Company.
TRAINING LAB
10) Trainee will get special Lecture on
“HOW TO FACE AN INTERVIEW” by
our Corporate Scientists and Scientific
Consultants.
TRAINING LAB

TRAINEES IN LAB TRAINEES IN LAB
TRAINEES IN LAB TRAINEES IN LAB
BIOINFO. LAB. BIOINFO. LAB.

PROJECTS IN CLONEGEN BIOTECHNOLOGY PVT. LTD.
Sr. No. TITLE
1. “A Study of antimicrobial activity of medicinal plants against various

multiple drug resistance pathogens and their molecular
characterization.”
2. “Isolation and Biochemical analysis of Alpha amylase isolated from

Bacillus amyloliquefaciens and optimization of culture conditions of the
Bacillus in stress conditions.”
3. “Effect of Chitinase Enzyme against plant pathogenic Fusarium and

Colletotrichum falcatum fungi, Isolation and Molecular Cloning of
Chitinase gene from Bacillus thuringiensis.”
4. “Isolation, Production & Purification of Antibiotics Isolated from

Microbes from Soil Sample”
5. “Isolation of genomic DNA from Chick Pea and its Molecular

Classification using RAPD markers”
6. “Isolation, Production and Characterization of Alpha-amylase from

Bacillus amyloliquefaciens by UV treatment.”
7. “A Study of Antimicrobial Activity of Domestic Medicinal Plants & it’s

Bioinformatics Analysis of Antibiotic Genes from Genomic Data Bases
With Degenerate Primer Prediction.”
8. “Studies on Bacteriocin Activity of Wild Type Gram Negative Strains

against Bacterial Strains Present in GI flora.”
9. “ Isolation, Production & Characterization of Beta-galactosidase from

Lactobacillus plantarum.”
10. “Isolation & Characterization of Bacteriocins from Gram Negative

Organisms from Soil & Sewage Samples.”
11. “Isolation of various human pathogenic bacteria from soil samples and
study of their multiple drug resistance.”
12. “Sex determination of Carica papaya Seedlings using RAPD markers
13. “Identification and Characterization of Oil degrading genes of

Pseudomonas sps.& Cloning of Oil degrading gene in E. coli”
14. “Evaluation of Biological Activities of an Actinomycetes Strain Isolated

from Plant Rizosphere.”
15. “Comparison of Production and Thermal Stability of Amylase Secreted

from Parent and Mutant Strains of B. amyloliquefaciens”

16. “Optimization of Media Composition for Increased Production of
Bacterial Extracellular Amylase”
17. “Enzyme Kinetic Studies on Amylases Produced from Bacillus

amyloliquefaciens after Mutation with UV irradiation and Chemical
Mutagen”
18. “Isolation and Identification of Protease Producing Bacteria from Soil

and Studies on its Properties”
19. ”Estimation of total phenol of herbal plants and its antioxidant

activity(One Method)”
20. “Estimation of total phenol of herbal plants and its antioxidant

activity (Three Method)”
21. ”Antihelmenthic activity of herbal plants”.
22. ”Antioxidant, antihelminthic and antimicrobial activity of herbal

plants”.
23. ”Isolation and characterization of antimicrobial components from

herbal plants”.
24. ”In-Vitro antioxidant activity of herbs”.
25. “Analysis of vaccine target for Dengue virus and identification of

immunodominant peptides” (BIOINFO. PROJECT)
26. “To predict primer for the amplification of gene responsible for the
production of protein for antimicrobial activity” (BIOINFO. PROJECT)
27. “Therapeutic analysis of swine flu for identification of immunodominant

peptides” (BIOINFO. PROJECT)
28. “In silico analysis of glycoprotein, neuraminidase, and hemagglutanin –

a comparative study on different strains of influenza virus A” (BIOINFO.
PROJECT)
29. “Pathways of evolution of influenza virus A and sequential studies”

(BIOINFO. PROJECT)
30. “Comparison of Ras protein (cancer functional protein) to check the

mutation” (BIOINFO. PROJECT)
31. “Population structure of gene encoding the polymorphic plasmodium

falciparum apical membrane antigen 1”(BIOINFO. PROJECT)
32. “Role of bioinformatics in computer aided drug design for acute

promyelocytic Leukemia” (BIOINFO. PROJECT)
33. “Role of bioinformatics in computer aided drug design for HIV/AIDS”

(BIOINFO. PROJECT)

34. “Comparing the pathogen status of two disease viruses of same genus”
(BIOINFO. PROJECT)
35. “Phylogenetic studies revealing existence of multiple lineage and

relationship between the Flavivirus group” (BIOINFO. PROJECT)
36. “A prospective epidemiologic study of dengue virus with other

flavivirus” (BIOINFO. PROJECT)
37. “Study antigenic peculiarities in west Nile Virus calculating nucleotide

change and deletions within the envelope glycoprotein gene” (BIOINFO.
PROJECT)
NOTE: Selected Trainees for CER-07 can choose any one of above projects during
Training Period. Clonegen Biotechnology will assist trainees to publish Research
Paper in International & National Scientific Journals based on their findings of
projects.
CONTACT US FOR ANY QUERY
INDIA OFFICE- USA OFFICE-
CloneGen Biotechnology Pvt. Ltd.

D-34, Sector -2,
NOIDA -201301
CloneGen Biotechnology LLC
Telephone Nos. –+91-120-4217675/676/677 177 Addis Dr.
Fax - +91-120-4217678 Churchville, PA 18966, USA.
Cell No. – +91-8800576628,8826028438
URL: http://www.clonegenbiotech.com
WORKING DAYS ‘MONDAY TO SATURDAY’

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INFORMATION BROCHURE

Industrial Training Certificate Program in “State of the Art Lectures

From The Training Director’s Pen

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 1

Lecture-1 Working in Biotech Lab

CORPORATE FACULTY & CONSULTANTS:

Dr. Ajit Srivastava Executive Director at Esperion Therapeutics, USA

Dr. Neelam Srivastava Research Scientists, Bristol-Myers Squibb, NJ, USA

Dr.T.B. Rajavashishtha Professor, UCLA, CA, USA

Mr. Ravi Jahagirdar Director, Pharmacology, Resverlogix, Canada

Prof Debomoy K Lahiri Inidana University School of Medicine, Indianapolis, USA

Dr Rangarajan Narayanan Former Research Scientist at Bristol-Myers Squibb, USA

Prof Khosrow Adeli Professor, University of Toronto, Canada

Prof Mahendra Thakur Professor of Zoology, Banaras Hindu University, Varanasi

Prof Maurizio Averna Professor of Medicine, Palermo University, Italy

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 2

SECTION -1A: MICROBIOLOGY

Sr. No. Content

SECTION -1B: MICROBIOLOGY(Newly Introduced)

Sr. No. Content

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SECTION -3A: BIOCHEMISTRY

SECTION -3B: BIOCHEMISTRY(Newly Introduced)

SECTION -4: CHROMATOGRAPHY TECHNIQUES

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 4

Sr. No. Content

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 5

1. Certificate Program in “Hands on Industrial Training in Advanced Biotechnology and

DURATION - One Month

DURATION - One & Half Month

2. Certificate Program in “Hands on Industrial Training in Advanced Biotechnology

DURATION - Two Months

DURATION - Two & Half Months

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 6

DURATION - Three Months

DURATION - Three & Half Months

4. Certificate Program in “Hands on Industrial Training in Advanced Biotechnology

DURATION - Three & Half Months

DURATION - Four Months

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 7

DURATION - One Month

DURATION - One & Half Month

6. Certificate Program in “Hands on Industrial Training in Advanced Biotechnology

DURATION - One Month

DURATION - One & Half Month

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 8

DURATION – Four months

4. Online Entrance Examination Fee – Rs. 550/-.

8. Certificate Program in "Hands-on Industrial Training and State-of-the-art

DURATION – Two Months

* In the collaboration of Cotec Pharma, Ghaziabad (http://www.cotecpharma.com)

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 9

DURATION – One Month

DURATION – Two Months

DURATION – Three Months

DURATION – Four Months

DURATION – Six Months

@ All Right Reserved to CloneGen Biotechnology Pvt. Ltd. 10

3. BIOINFORMATICS DATABASE & TOOLS: 4 SEQUENCE ALIGNMENT:

(a) Types of Database (a) Introduction

5. STRUCTURAL ALIGNMENT: 6 STRUCTURE PREDICTION

(a) Protein Structure alignment (a) Introduction