The SAS System 19:17 Saturday, March 19, 2011 1

raw data

Obs pro lip lre

1 1 1 48.99
2 1 1 49.75
3 1 1 50.79
4 1 2 39.23
5 1 2 40.86
6 1 2 40.31
7 1 3 32.87
8 1 3 33.58
9 1 3 32.44
10 2 1 50.41
11 2 1 52.01
12 2 1 49.76
13 2 2 45.14
14 2 2 46.95
15 2 2 45.94
16 2 3 34.44
17 2 3 34.78
18 2 3 35.27
19 3 1 42.86
20 3 1 41.57
21 3 1 41.96
22 3 2 37.06
23 3 2 38.53
24 3 2 37.73
25 3 3 29.77
26 3 3 30.40
27 3 3 30.91

The SAS System 19:17 Saturday, March 19, 2011 2

summary statistics by treatment

------------------------------------------- protein=1 -------------------------------------------

The MEANS Procedure

Analysis Variable : lre lipid retention

N Mean Std Dev

ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ
9 40.98 7.37
ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ

------------------------------------------- protein=2 -------------------------------------------

Analysis Variable : lre lipid retention

N Mean Std Dev

ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ
9 43.86 7.11
ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ

------------------------------------------- protein=3 -------------------------------------------

 Analysis Variable : lre lipid retention

N Mean Std Dev

ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ
9 36.75 5.19
ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ

The SAS System 19:17 Saturday, March 19, 2011 3

summary statistics by block

-------------------------------------------- lipid=1 --------------------------------------------

The MEANS Procedure

Analysis Variable : lre lipid retention

N Mean Std Dev

ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ
9 47.57 4.17
ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ

-------------------------------------------- lipid=2 --------------------------------------------

Analysis Variable : lre lipid retention

N Mean Std Dev

ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ
9 41.31 3.74
ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ

-------------------------------------------- lipid=3 --------------------------------------------

Analysis Variable : lre lipid retention

N Mean Std Dev

ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ
9 32.72 2.00
ƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒ

The SAS System 19:17 Saturday, March 19, 2011 4

profile plot of the raw data

Plot of lre*pro$lip. Symbol points to label.

‚
‚
55 ˆ
‚
‚
‚
‚ >1
‚
‚ >1 >1
50 ˆ > 1 >1
‚ >1
‚
l ‚
i ‚ >2
p ‚
 i ‚ >2
d 45 ˆ >2
‚
r ‚
e ‚ >1
t ‚ >1
e ‚ >1
n ‚ >2
t 40 ˆ > 2
i ‚ >2
o ‚ >2
n ‚ >2
‚ >2
‚
‚
35 ˆ 23
‚ >3
‚ >3
‚ >3
‚ >3
‚
‚ 23
30 ˆ >3
‚
Šƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒ
1 2 3

protein

The SAS System 19:17 Saturday, March 19, 2011 5

Proc anova Analysis

The ANOVA Procedure

Class Level Information

Class Levels Values

pro 3 123

lip 3 123

Number of Observations Read 27

Number of Observations Used 27

The SAS System 19:17 Saturday, March 19, 2011 6

Proc anova Analysis

The ANOVA Procedure

Dependent Variable: lre lipid retention

Sum of
Source DF Squares Mean Square F Value Pr > F

Model 4 1229.972578 307.493144 126.03 <.0001

Error 22 53.676222 2.439828

Corrected Total 26 1283.648800

 R-Square Coeff Var Root MSE lre Mean

0.958185 3.853923 1.561995 40.53000

Source DF Anova SS Mean Square F Value Pr > F

pro 2 229.649756 114.824878 47.06 <.0001

lip 2 1000.322822 500.161411 205.00 <.0001

The SAS System 19:17 Saturday, March 19, 2011 7

Proc anova Analysis

The ANOVA Procedure

Tukey's Studentized Range (HSD) Test for lre

NOTE: This test controls the Type I experimentwise error rate.

Alpha 0.05
Error Degrees of Freedom 22
Error Mean Square 2.439828
Critical Value of Studentized Range 3.55259
Minimum Significant Difference 1.8497

Comparisons significant at the 0.05 level are indicated by ***.

Difference
pro Between Simultaneous 95%
Comparison Means Confidence Limits

2-1 2.8756 1.0258 4.7253 ***

2-3 7.1011 5.2514 8.9508 ***
1-2 -2.8756 -4.7253 -1.0258 ***
1-3 4.2256 2.3758 6.0753 ***
3-2 -7.1011 -8.9508 -5.2514 ***
3-1 -4.2256 -6.0753 -2.3758 ***

The SAS System 19:17 Saturday, March 19, 2011 8

Proc anova Analysis

The ANOVA Procedure

Tukey's Studentized Range (HSD) Test for lre

NOTE: This test controls the Type I experimentwise error rate, but it generally has a higher
Type II error rate than REGWQ.

Alpha 0.05
Error Degrees of Freedom 22
Error Mean Square 2.439828
Critical Value of Studentized Range 3.55259
Minimum Significant Difference 1.8497
 Means with the same letter are not significantly different.

Tukey Grouping Mean N pro

A 43.8556 9 2

B 40.9800 9 1

C 36.7544 9 3

The SAS System 19:17 Saturday, March 19, 2011 9

Proc glm Analysis

The GLM Procedure

Class Level Information

Class Levels Values

pro 3 123

lip 3 123

Number of Observations Read 27

Number of Observations Used 27

The SAS System 19:17 Saturday, March 19, 2011 10

Proc glm Analysis

The GLM Procedure

Dependent Variable: lre lipid retention

Sum of
Source DF Squares Mean Square F Value Pr > F

Model 4 1229.972578 307.493144 126.03 <.0001

Error 22 53.676222 2.439828

Corrected Total 26 1283.648800

R-Square Coeff Var Root MSE lre Mean

0.958185 3.853923 1.561995 40.53000

Source DF Type I SS Mean Square F Value Pr > F

pro 2 229.649756 114.824878 47.06 <.0001

lip 2 1000.322822 500.161411 205.00 <.0001

Source DF Type III SS Mean Square F Value Pr > F

pro 2 229.649756 114.824878 47.06 <.0001

lip 2 1000.322822 500.161411 205.00 <.0001
 The SAS System 19:17 Saturday, March 19, 2011 11
Proc glm Analysis

The UNIVARIATE Procedure

Variable: resid

Moments

N 27 Sum Weights 27
Mean 0 Sum Observations 0
Std Deviation 1.43682639 Variance 2.06447009
Skewness 0.1348706 Kurtosis -0.9109732
Uncorrected SS 53.6762222 Corrected SS 53.6762222
Coeff Variation . Std Error Mean 0.27651737

Basic Statistical Measures

Location Variability

Mean 0.00000 Std Deviation 1.43683

Median -0.29778 Variance 2.06447
Mode . Range 5.29889
Interquartile Range 2.25000

Tests for Location: Mu0=0

Test -Statistic- -----p Value------

Student's t t 0 Pr > |t| 1.0000

Sign M -0.5 Pr >= |M| 1.0000
Signed Rank S 1 Pr >= |S| 0.9814

Tests for Normality

Test --Statistic--- -----p Value------

Shapiro-Wilk W 0.974328 Pr < W 0.7184

Kolmogorov-Smirnov D 0.109689 Pr > D >0.1500
Cramer-von Mises W-Sq 0.050112 Pr > W-Sq >0.2500
Anderson-Darling A-Sq 0.265984 Pr > A-Sq >0.2500

Quantiles (Definition 5)

Quantile Estimate

100% Max 2.773333

99% 2.773333
95% 2.318889
90% 1.967778
75% Q3 1.117778
50% Median -0.297778

The SAS System 19:17 Saturday, March 19, 2011 12

Proc glm Analysis

The UNIVARIATE Procedure

Variable: resid
 Quantiles (Definition 5)

Quantile Estimate

25% Q1 -1.132222
10% -1.831111
5% -2.221111
1% -2.525556
0% Min -2.525556

Extreme Observations

------Lowest----- -----Highest-----

Value Obs Value Obs

-2.52556 10 1.45778 26
-2.22111 8 1.73333 2
-1.83111 9 1.96778 27
-1.60333 22 2.31889 14
-1.44556 12 2.77333 3

Stem Leaf # Boxplot

28 1 |
2 03 2 |
1 57 2 |
1 0013 4 +-----+
0 58 2 | |
0 24 2 | + |
-0 3 1 *-----*
-0 998755 6 | |
-1 431 3 +-----+
-1 86 2 |
-2 2 1 |
-2 5 1 |
----+----+----+----+

The SAS System 19:17 Saturday, March 19, 2011 13

Proc glm Analysis

The UNIVARIATE Procedure

Variable: resid

Normal Probability Plot

2.75+ ++*
| ++*+
| *+*
| ***+*
| ***+
| +**+
| +***
| ****
| *+**
| *+*
| +*++
-2.75+ *++
 +----+----+----+----+----+----+----+----+----+----+
-2 -1 0 +1 +2

The SAS System 19:17 Saturday, March 19, 2011 14

Proc glm Analysis

Plot of resid*pro. Legend: A = 1 obs, B = 2 obs, etc.

resid ‚
3ˆ
‚
‚ A
‚
‚
‚ A
‚
2ˆ A
‚
‚ A
‚
‚ A
‚ A
‚ A
1ˆ A A
‚ A
‚
‚ A
‚ A
‚
‚ A
0ˆ
‚
‚ A
‚ A A
‚
‚ A A
‚ A
-1 ˆ A
‚ A
‚ A
‚ A
‚ A
‚
‚ A
-2 ˆ
‚
‚ A
‚
‚ A
‚
‚
-3 ˆ
Šƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒ
1 2 3

protein

The SAS System 19:17 Saturday, March 19, 2011 15

Proc glm Analysis
 Plot of resid*lip. Legend: A = 1 obs, B = 2 obs, etc.

resid ‚
3ˆ
‚
‚ A
‚
‚
‚ A
‚
2ˆ A
‚
‚ A
‚
‚ A
‚ A
‚ A
1ˆ A A
‚ A
‚
‚ A
‚ A
‚
‚ A
0ˆ
‚
‚ A
‚ A A
‚
‚ B
‚ A
-1 ˆ A
‚ A
‚ A
‚ A
‚ A
‚
‚ A
-2 ˆ
‚
‚ A
‚
‚ A
‚
‚
-3 ˆ
Šƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒ
1 2 3

lipid

The SAS System 19:17 Saturday, March 19, 2011 16

Proc glm Analysis

Plot of resid*yhat. Legend: A = 1 obs, B = 2 obs, etc.

resid ‚
3ˆ
‚
‚ A
 ‚
‚
‚ A
‚
2ˆ A
‚
‚ A
‚
‚ A
‚ A
‚ A
1ˆ A A
‚ A
‚
‚ A
‚ A
‚
‚ A
0ˆ
‚
‚ A
‚ A A
‚
‚ A A
‚ A
-1 ˆ A
‚ A
‚ A
‚ A
‚ A
‚
‚ A
-2 ˆ
‚
‚ A
‚
‚ A
‚
‚
-3 ˆ
Šƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒƒƒƒƒƒƒƒƒƒƒƒˆƒƒ
25 30 35 40 45 50 55

Yhat

GPW5-773716-RAG-8593