5/20/2010

IDENTIFICATION, CHARACTERIZATION
AND MANAGEMENT OF FUSARIUM WILT
OF BANANA (SABA, LAKATAN, LATUNDAN)

Teresita U. Dalisay
Crop Protection Cluster, UPLB

INTRODUCTION

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Symptoms of Fusarium wilt of Banana

(a)Yellowing starts at the

margin of the leaves

(b) Dried leaves hang in a

skirt like manner

(
a b

(c & d) lengthwise section of

pseudo stem
d showing discoloration
(e) cross section of pseudo
stem showing
discoloration around the
core.
e
c

OBJECTIVES
1. To map incidence of Fusarium wilt in
banana major growing areas in Luzon
2. To map the occurrence of the different
races of Foc across regions of Luzon
3. To have repository isolates and herbaria
of Foc
4. To do management of banana against
the disease

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OBJECTIVE 1 - To map the incidence of

Fusarium wilt in banana
major growing areas in Luzon
A1 – 7 regions in Luzon; 2 regions in Visayas
YEAR 1 YEAR 2 YEAR 3
Surveyed areas: Surveyed areas: Surveyed areas:
Quezon 11 b 8 t Pangasinan 15 b 9 b Benguet 3b 1t
Cavite 3 b 2t Aurora 2b 1 t Cam. Sur 8 b 4 t
Laguna 4 b 3 t
La Union 2b 1t Negros Or. 12 b 8 t
Batangas 6 b 4 t
Isabela 3t Bohol 4b 4t
Or. Mind. 4 b 2 t
Quirino 2t
Nueva Viscaya 3t
Aurora 2b 1t

Total: 28 b 19 t 21 b 20 t 27 b 17 t

Grand total: 76 b
56 t

A2 – mini plot survey of foliar diseases

DISEASE MADDELA NAGTPUNAN

Div. Norte Ysmael Dismungal Sangbay
Sigatoka 44.56 93.26 88.67 49.42
Bunchy top 0.34 5.62 5.52 15.95
Cordana 72.45 82.02 67.15 56.81
Leaf cross 52.04 77.53 88.08 53.31
Freckle 64.97 44.10 29.65 26.07
Wilt 0.00 0.28 0.58 0.00
Total plants 294 356 344 257

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OBJECTIVE 2 - To map occurrence of the

different races of Foc across regions
of Luzon

AREA LATITUDE LONGITUDE DATE TIME

Villaverde, Nueva Vizcaya 16.48461520672 121.13742113113 4/17/2007 18:11:00

Solano, Nueva Vizcaya 16.54325366020 121.18974030018 4/18/2007 11:58:00

Villaverde, Nueva Vizcaya 16.59018158913 121.18637144566 4/18/2007 12:41:00

Bagabag, Nueva Vizcaya 16.59020841122 121.18634998798 4/18/2007 12:39:00

Alicia, Isabela 16.85044705868 121.75169527531 4/21/2007 14:45:00

Alicia, Isabela 16.93962514400 121.74560666084 4/21/2007 17:34:00
Ramon, Isabela 16.73891007900 121.54689252377 4/23/2007 13:16:00
Cordon, Isabela 16.63021624088 121.50713145733 4/23/2007 20:19:00
Diffun, Quirino 16.46624743938 121.52022063732 4/24/2007 13:20:00
Cabarroguis, Quirino 16.43646955490 121.51280164719 4/24/2007 13:47:00

Cabarroguis, Quirino 16.54044806957 121.54110968113 4/25/2007 12:16:00

Diffun, Quirino
16.65471553802 121.48301303387 4/25/2007 16:15:00

A3- Positions of Areas with the presence

of Fusarium wilt

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OBJECTIVE 3- To have repository isolates

and herbaria of Foc

A1 : total no. of isolates

First year : 28
Second year: 21
Third year: 27
Total: 76
A2: total no. of dried pseudostems
= 50

VCG analysis for 1st batch of samples

CAV OTHER VCG SEQ. ID

CAV 1832 CT2517 Nit 1 Grp 3

CAV 1834 QP1813 Nit1 Grp 3

CAV 1837 LP112 Nit1 Grp 3

CAV1840 QD1-11-11 Vcg incom. Grp 3

CAV1839 BT1422 Vcg B Grp 4

CAV1833 QP2514 New vcg/npath Grp 5

CAV1838 BT1122R1 New vcg/npath Grp5

CAV1830 BM1225 Unk., not an Foc Outside foc phylo

CAV1842 BL1311 Unk., not an Foc Outside foc phylo

CAV1835 LP1111R3 Nit 1 Seq

CAV1836 LP115R3 nitM Seq

C1V1843 LP1111 Vcg incom. Seq

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OBJECTIVE 4 - To do management of
Fusarium wilt

by

UTILIZATION OF ENDOPHYTIC FUNGI

A 1 : - Collecting sites and distribution of

endophytic fungi

Total no. of EF isolates: 54

Distribution: 42.59% (Davao del Norte)

22.22% (Quirino)
16.67% (Gagayan)
12.96% (Nueva Vizcaya)
11.11% (Isabela)
7.41 %(Laguna)

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Cagayan (16.67%)
(EF nos. 46,47, 48, 49
50, 51, 52, 53, 54)

Isabela (11.11%)
(EF nos. 1, 2, 3, 4,5,6)

Nueva Vizcaya (12.96%)

(EF nos. 2, 8, 10, 11,
12, 13, 14)

Quirino (22.22%)
(EF nos. 1, 2, 3, 5, 8, 16, 17, 18, 19,
20, 21, 22)

Laguna (7.41%)
(EF nos. 7, 8, 9, 15)

Davao del Norte (42.59%)

(EF nos. 23, 24, 25, 26
27, 28, 29, 30, 31, 32, 33, 34, 35,
36, 37, 38, 39, 40, 41, 42, 43, 44,
45)

Percent abundance of endophytic fungi isolated from Luzon

Percent abundance

40 35.48
35
30
25
19.35
20 16.73
15
10 6.45 6.45
5 3.22 3.22 3.22 3.22 3.22
0

Endophytic fungi

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Percent abundance of endophytic fungi isolated from Mindanao

Percent abundance

30 26.09 26.09
25
20
15 13.04 13.04
8.7
10
4.35 4.35 4.35
5
0

Endophytic fungi

A 2 – new records of endophytic fungi on

banana (Codineae; Peyronellaea
spp.)

A3 - identified promising Ef isolates

against Foc

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A2 – Two promising isolates namely

Aspergillus sp., EF25, EF26 were
able to reduce colony diameter of
Foc isolates

Aspergillus sp. EF1 (AMR)

growing on hsPDA, obverse
(A), reverse (B), long and
slender conidiophores bearing
clavate vesicle
C) (400X), long chain of
conidia attached to a vesicle
(D and E) (440X),
unicellular, globose conidia (F)
(1000X).

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Aspergillus sp. EF25

(AMR) growing on
hsPDA, obverse (A),
reverse (B),
micronematous mycelia
(C) (400X), straight
conidiophore
with mass of spores (D)
(400X), vesicle fully
covered with
phialides (E) (400X),
unicellular, globose
conidia (F) (1000X).

Aspergillus sp. EF26

(AMR) growing on
hsPDA, obverse (A),
reverse (B), young and
mature fruiting bodies
(C) (400X), vesicle fully
covered with phialides
(D) ),clavate vesicle with
long phialides bearing
catenate conidia (E)
(400X), unicellular,
globose conidia (F)
(1000X).

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Foc-NV isolates grown alone on agar plates (A-NV2A, D-NV3B,G-NV5C)

compared when grown with Aspergillus sp. EF1 (B, E, H) and Aspergillus
sp. EF25 (C, F, I). Rapid colony forming characteristics of Aspergillus sp.
EF1 completely overgrew Foc isolates, except Aspergillus sp. EF1
against NV2A and NV3B.

Foc-IS isolates grown alone on agar plates (A-IS3B, C-IS5A, E-

IS6B) compared when grown with Aspergillus sp. EF26 (B, D, F).
EF completely covered Foc isolates except Foc-I6B.

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Foc-QU isolates grown alone on agar plates (A-QU1A, C-QU2B,

E-QU3A) compared when with Aspergillus sp. EF26. Note
restricted growth of Foc- QU2B and Foc-QU3A isolates .

IN-VIVO EVALUATION OF EF

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Response of banana on EF treated soil.

ISOLATE OF EF26 EF25
FOC
ISABELA IS3B 29.30 bc 33.43c
IS5A 31.01bc 32.50c
IS6B 36.83c 40.47d
NUEVA NV2A 25.73b 31.45bc
VIZCAYA
NV3B 30.17bc 35.77bc
NV5C 29.60bc 30.63c
QUIRINO QU1A 23.73b 24.03c
QU2B 25.10b 27.60b
QU3A 25.17b 28.30b
C1 (Foc alone) 8.26a 13.60bc
C2 (EF alone) 30.0bc 28.33abc

Response of banana
plants on activity of
Aspergillus sp.
EF26 against Foc isolates
from Isabela. C1 refers to
FOC-IS isolates alone
while C2 refers to EF26
alone.

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Response of banana plants

on activity of Aspergillus
sp. EF26 against Foc
isolates fromNueva
Vizcaya. C1 refers to FOC-
NV isolates alone
while C2 refers to EF26
alone.

Response of banana
plants on activity of
Aspergillus sp. EF26
against Foc isolates from
Qurino. C1 refers to
FOC-QU isolates
alone while C2 refers to
EF26 alone.

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ACKNOWLEDGEMENT
Isabela– - Dr. Billy Timanel
- Dr. Flor Timanel
– Mr. Ruel Gesmundo
Quirino – Prof. Fredisminda
Dolojan
– Ms. Luzviminda
Sabigan
Nueva Viscaya – Dr. Jonar Yago
Aurora – Ms. Zenaida Abawa
Negros Oriental – Dr. Peter Dayot
– Dr. Nancy Olasiman
– Prof. Al Zamora

ACKNOWLEDGEMENT
Pangasinan – Prof. Dannah Jean
Concepcion
Benguet & La Union – Dr. Janet Luis
– Ms. Marian Luis
Oriental Mindoro – Mr. Jess Reyes
- Mr. De Sagun
Bohol - Dr. Liza Quirog
Quezon - Dr. Edna Aguilar

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GPS rental – Dr. Demie

Macandog
Travel companion – Ms. Jennifer
Niem
– Dr. Edna
Ardales

THANK YOU!

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