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The first part of this lecture is taken from our text book (Burton’s
Microbiology for the Health Sciences, 9th Ed,chapter 10)
The other part is taken from (Jawetz, Melnick, & Adelberg's Medical
Microbiology,24th, Chapter 11)
It is mentioned earlier in the course that most of the microbes are non-
pathogenic for human or animal , only a small percentage are pathogenic, and
in fact most relationships between humans and microbes are beneficial to
human rather than being harmful.
So all microorganisms whether they are bacteria, fungi, yeast, etc..that
present both on and in our bodies are called indigenous micro flora (normal
flora).
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Symbiosis can exist in 4 forms :
1. Neutralism:
Two organisms live in a compact relationship in which neither of them is
interacting with the other one or affecting its goal, so basically the two
organisms are present in the same place and neither symbionts is affected by
the relationship.
2. Commensalism :
Two organisms live in the same place; one organism is taking benefits of
the other one, while the last is not affected by this relationship.
For example: Many microorganisms that live in your GIT or in your skin are
considered to be commensals ,because the human body provide the
nutrients ,proper temperature and moisture to them; however, they will not
cause any infection to it.
4. Parasitism:
In this relationship one organism lives in the extent of another organism,
causing a harmful effect for it.
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a little harm and no clinical manifestations will appear ,so the disease is
ataxonomic .
**** Important point about all the types of Symbiotic relationships is that:
Any disturbance in the conditions due to the application of alcohol or taking
anti-microbial agents might shift the relationship from Commensalism to
Parasitism relationship, for example, and so on.
So a key point is that any changes in conditions can cause one type of
symbiotic relationship to shift to another type ,that’s why sometime the
component of normal flora which are usually commensals turns to
pathogenic.
A Summary
Slide No.8:
The picture shows the areas of the body where most of the indigenous
Micro flora reside.
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1-skin: the biggest organ in the human body that has high level of bacteria
2- Gastro intestinal tract (GIT)
3- Upper respiratory tract
4- Urogenital tract
5- Eyes and the external ears
****The lower respiratory tract is usually sterile so the bronchi, the small
bronchioles and the alveoli are usually sterile.
Slide No.9"Constituents of Normal Flora:"
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Also it will produce metabolic or toxic products.
And it may produce some antibiotics.
**By doing all that it can prevent the binding and the colonization of
pathogenic bacteria.
So the bacterial interference is a very important mechanism to prevent the
human infection.
** Removal or disturbance of NF might lead to problems due to rapid
colonization by other organisms which are called (opportunists pathogens) so
might end up with a disease.
Slide No.12:
If the NF components are introduced into a new site such as blood or deep
tissue it may cause an infection.
For example; Viridans streptococci (that presents in the mouth); if you
extract your tooth it can enter the blood circulation, might attach and colonize
at the heart valve leading to what is called infective endocarditis
Alternatively if the bacteria get into the deep tissue or normal sterile site they
can lead to infection
For example; Bacteroides vragilis (that is found in the large intestine of the
human body) might enter the peritoneal cavity if there is a trauma or an
accident or someone bunches you in the stomach, and some of this bacteria
will enter the peritoneal cavity then it will multiply there very quickly and
cause purulent infection and bacteremia.
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Slide No.14, 15 "Skin NF:"
The skin is not an easy place to colonize by bacteria, because there are
many factors that inhibit bacterial colonization which are:
1. Low pH
2. Fatty acids from sebaceous secretions (some of the fatty acids have anti-
microbial activity)
3. Lysozymes that degrade the cell wall of bacteria.
***So not any microorganism that introduce into the skin can colonize there.
A key point in skin normal flora is that it differs by different locations
due to:
1- Different anatomical location might have slightly different numbers of
the normal flora according to different moisture secretion that can change the
components of the normal flora.
2- Places of wearing of clothes can change skin components
3- Proximity to mucous membranes (mouth, nose, perineal areas have
components of the normal flora that are derived by the mucous membrane)
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Corynebacteria.
Staphylococci (S. epidermidis, S. aureus)
Streptococci.
As we are the future dentists , it is important for us to know how the dental
caries are formed.
The tooth consists of enamel layer, dentin, pulp, and cement layer.
The dental caries evolve 2 things:
1. Demineralization of enamel.
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Slide No.19, 20 " Role of NF in Dental Caries:"
The esophagus usually contains bacteria that are derived from saliva and
food, although it is not a good place for infections.
Once the food enters the stomach most of the bacteria will be destroyed
due to very low PH and high acidity.
If you want to count the number of bacteria in the stomach you will find
only 1000 to 100000 gram of bacteria per food content.
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***Once the food get to the stomach not many organisms can withstand its
high acidity (pH=2).
Slide No. 23" Normal Flora of the Intestines:"
Once the food exit from the stomach it will mix with the bile in the small
intestine that will drive the PH to increase slightly, so now bacteria can grow
and multiply much more quickly.
In the Duodenum (the first part of the small intestine) you can find 103-106
bacteria per gram of contents.
As the food gets down in the small or the large intestine the number of
bacteria will increase very quickly.
So in Jejunum and ileum there are 105-108 bacteria per gram of content.
In the cecum and transverse colon 108-1010 bacteria per gram of content.
And finally Sigmoid colon and rectum contains almost 1011 bacteria per
gram of content.
GIT has various numbers of normal flora; the most important is a group
of bacteria that is called Enterobacteriaceae.
Some members of Enterobacteriaceae are not present in the normal flora and
are only present if you have an infection with these bacteria; so Salmonella,
Shigella, Yersinia are members of Enterobacteriaceae but they aren’t
members of the normal flora that present in the healthy gastro intestinal tract,
but they present in case of infection.
So these Enterobacteriaceae are facultative anaerobic: they can use the
oxygen if present and can live without oxygen ,also there are anaerobic
bacteria in the small and large intestine and these are in extremely high
numbers.
There are 1000 time more anaerobic bacteria than the facultative
anaerobic bacteria. The most important example about anaerobic bacteria that
the Dr wants us to memorize is Bacteroides fragilis.
Another example is Clostridum perfringens, which is the main cause of
gas gangrene in human, so if these bacteria introduce to a deep tissue it can
cause gangrene.
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Slide No. 26" Normal Flora of the Intestines:"
Group B streptococci are very important because they are the cause of
neonatal infection.
If a women carry this bacterium gives birth to a baby, the bacteria will
spread to the baby and will cause a very severe infection that will lead to
neonatal sepsis and meningitis which is highly mutant.
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Normal Flora of the Conjunctiva()ملتحمة العين:
The End
Done By: Enas Salameh
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