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Thesis submitted to the Department of Animal Breeding and Physiology, in partial fulfilment
of the requirements for the Degree of Doctor of Philosophy (Animal Breeding and
MARCH 2011
i
DECLARATION
I declared that the work described in this thesis represents my original work and has not
been previously submitted to any university or similar institution for any degree
Signature of candidate:--------------------------------------------------------
Date:-------------------------------------------------------------------------------
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Name of Candidate:
Matric Number :
Certification
We the under- signed, hereby certify that this Thesis by the above named candidate be
accepted as fulfilling part requirement for the degree of Doctor of Philosophy (Animal
breeding and Genetics), University of Agriculture, Makurdi.
……………………………….. ……………………………………
Prof. Dr. N. I. Dim Date
Major Supervisor.
……………………………………. ………………………………………….
Dr. O. M. Momoh Date
Minor Supervisor.
……………………………………. …………………………………………….
Prof. I. I. Bitto Date
Minor Supervisor.
………………………………….. ………………………………………………
Prof. Dr. N. I. Dim Date
Head of Department.
………………………………….. ………………………………………………..
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ABSTRACT
The indigenous Muscovy duck (Cairina moschata) in Nigeria is yet to be adequately
evaluated and classified into morphotypes. Its performance, morphology and genetic
diversity are yet to be ascertained. Genetic characterization of Muscovy ducks collected from
two agro- ecological zones of Nigeria (guinea savannah and rain forest) were carried out
using two approaches. First was morphological diversity assessment of the two ecotypes
using On-farm data and secondly On-station evaluation of performance, genetic and
molecular assessment of the two populations, which were carried out at the Poultry Unit of
College of Agriculture, Lafia, Nasarawa State and Centre for Biotechnology Research and
Training, ABU, Zaria. The general objective of the study was to evaluate the phenotypic and
genetic characteristics of the Nigerian indigenous Muscovy duck from the two agro-
ecological zones with a view to highlighting its potentials to contribute to egg and meat
production in Nigeria. For the On-farm data, 12 zoometric traits were measured on 680
ducks from the two populations, principal component factor analysis and discriminate
between the two populations and also between sexes. Factors analysis was used to assess
inter-relationship between traits in a pooled data between sexes. Seven morphometric traits
had discriminatory power that distinguished the two populations. Squared Mahalanobis
distance between the two populations was 2.963 and significant (P<0.001). For on-station
experiment, 192 pedigree day-old ducklings of guinea savannah ecotype obtained from 10
sires and 60 dams and 160 ducklings of rainforest ecotype also from 10 sires and 60 dams
hatched in 6 separate batches by natural incubation were used. The ducklings from the two
populations were brooded and reared on deep litter pen from 0-20 weeks. Body weight
(BWT) at 0, 1, 3, 5, 10, and 20 weeks were evaluated. Body weight gain and average daily
gain were measured at 5 weekly internal in the two ecotypes up to 20 weeks. At 20 weeks, 60
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ducks of each population were randomly selected for egg laying performance and monitored
for first cycle and second cycle egg production. Body weight was significantly higher
(P<0.05) in the guinea savannah ecotype between 0-10 weeks of age but not significant at
adult age. Sex significantly (P<0.05) affected body weight from 3 to 20 weeks of age in both
populations. There was significant difference between the guinea and rainforest ecotypes with
the rainforest ecotype having heavier body weight at first egg. Heritability estimates for
growth traits of the two populations did not differ significantly, all were low to moderate.
That of body weight gain and average daily gain increased with age in both populations. In
all heritability estimates for early performance were high in the guinea savannah ecotype,
while higher for the rainforest ecotype at late ages. From this results it can be concluded that
selection for performance can be applied in early stage for the guinea savannah ecotype
while at later age for the rainforest. Sexual dimorphism was clearly exhibited in the two
populations. On molecular assessment high similarity was obtained between the populations
(0.86%), genetic distance was small 0.14, suggesting that they have common ancestor and
evolved little adaptive variation as a result of isolation. In all no wide variation existed
between the ecotypes, which was evident both at phenotypic and molecular levels an
indication of little environmental influence on the duck breed. The ecotypes can be selected
for both meat and egg production. More research is suggested using more sophisticated
molecular approach to ascertain genetic potentials and diversity of the Nigerian indigenous
muscovy duck
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ACKNOWLEDGEMENTS
First and foremost, I would like to express my sincere thanks to my major supervisor- Prof.
Dr. N.I. Dim. I greatly appreciate the meticulous guidance, patience, encouragement,
leadership, support and the conducive environment that he created for me to complete my
Momoh- for his valuable support, encouragement and technical guidance during the course of
this study.
My sincere thanks goes to the former Head of Department of Animal Breeding and
Physiology and my second co- supervisor, Prof. I.I. Bitto, and the Dean, College of Animal
Science- Prof. O.I.A. Oluremi for their advice and encouragement during the course of my
work.
I wish to thank the staff of the Department of Animal Science and Livestock Unit, College of
Agriculture Lafia, Teaching and Research Farm, especially Mathias B. Bello, Gwanto, and
host of others for their help and support during the three-year performance evaluation
studies. I would like to express my thanks to all the farmers who allowed me to use their
ducks for morphological evaluation in Cross River, Akwa Ibom, Benue, Nasarawa and
Niger states. Special thanks goes to Edidion Akpan Charles who was of enormous support to
me during the field data collection, purchase and transportation of ducks from the rain forest
zone to Lafia.
Many thanks to Dr. P.A. Wuyep of the Centre for Biotechnology Research and Training,
ABU, Zaria for performing the molecular analysis and Abdulmojeed Yakubu of the Faculty
of Agric, Nasarawa State University, Lafia Campus who has been so inspirational, he
Sincere thanks to members of Olam Rice Extension Programme, Makurdi, for their individual
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It is imperative that I recognise the encouragement of my friends, brother and sisters in the
course of the study, Idris D. Hassan, Daikwo I. Silvesters, Jacob Enogela Okpadobu, Louis
Ogah, Rita and Omogbele, Bala, Yakubu and all others. I say thank you.
Special thanks to Dr. Maikano M. Ari and Dr Chris Iyimogah for their encouragements.
To my parents -Mr. John Ogah (Ogbole gi Doma), Patrick Ogah (Dan Moyin Doma) Hajiya
My wife Gambo Ogah and my children Florence, Phyllis, Peace and Newly born Akawu
Abednego, I remain grateful for your prayers and understanding throughout the period of
my studies
To ALMIGHTY GOD who by His Grace granted me “everything” Honour and adoration
are yours.
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TABLE OF CONTENTS
Title page…………………………………………………………………………….i
Declaration…………………………………………………………………………..ii
Signature page……………………………………………………………………….iii
Abstract…………………………………………………………………………… .. iv
Acknowledgements………………………………………………………………….vi
Table of contents…………………………………………………………………….viii
List of figures…………………………………………………………………………xiv
List of tables……………………………………………………………………… … xv
1.0 INTRODUCTION…..……………………………………………………1
2.2.2.6 Hatchability……………………………………………………………….. 16
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2.2.2.7 Hatching weight of duckling……………………………………………. 18
2.2.211 Incubation………………………………………………………………….21
reproductive traits)………………………………………………….. 25
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2.5.2 Random Amplified Polymorphic DNA-PCR application in ducks…… 46
3.2 On-station…………………………………………………………………… 48
3.2.3.3 Medication…………………………………………………………………… 50
3.4.2 Phenotypic evaluation of the morphological and growth traits of the ecotypes…55
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3.5 Molecular characterization……………………………………………………. 62
4.0. RESULTS…………………………………………………………………….68
4.2.3 Body weight gain and average daily gain (BWG and ADG)…………… 97
4.3.5 Genetic and phenotypic correlation between body weight body weight
104
gain and average daily gain at specific ages……………………………
4.4.1 Body weight and first egg, average age at first egg, number of egg laid
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..the first laying cycle……………………………………………… 117
4.5.2 Genetic and phenotypic correlation of external egg quality traits of the
5.3.4 Genetic and phenotypic correlation between BWT, BWG and ADG… 137
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5.4.2 Performance at second laying cycle…………………………………….. 140
5.4 .4 Genetic and phenotypic parameters of laying and egg traits…………… 141
5.4.4.1 Heritability of laying performance and external egg traits ……………… 141
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LIST OF FIGURES
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LIST OF TABLES
sex………………………………………………………………………… 71
measurements …………………………………………………………. 75
6. Eigen values and share of total variance along with factor loading after
muscovy duck…………………………………………………………… 77
8. Eigen value and shares of total variance along with factor loading after
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13. Original classification matric obtained by the application of non-
16. Least squares means for body weight0-20 weeks of the two ecotypes of
17. Least squares means values of body weight by sex of Muscovy ducks
ecotype…………………………………………………………………… 95
weight along with degree of sexual dimorphism for the rain forest
ecotype…………………………………………………………………… 96
20. Least squares means for body weight gain and average daily gain by
ecotypes………………………………………………………………….. 98
21. Least squares means standard errors of body weight gain and average
23. Heritability estimates of 5-weekly body weight gain from sire variance
24. Heritability estimates of 5 weekly average daily weight gain from sire
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25. Phenotypic correlation of the body weight of the two indigenous
26. Genetic and phenotypic correlations between body weight, body wieght
gain and average daily gain of the two ecotypes…………… ………….. 108
27. Least square means ±SE of body weight at first egg, age at first egg,
average number of egg laid at first laying cycle, average egg weight and
28. Least sqaures means ±SE of egg characteristics, weight, length and width
29. Least sqaures means ±SE of second laying characteristics of the ecotype 114
30. Laying intensity based on laying season of the guinea and rainforest
ecotypes…………………………………………………………………… 116
31. Heritability estimates from sire variance components for the first laying
32. Genetic and phenotypic correlations of external egg quality traits for the
components………………………………………………………………. 121
36. Number of RAPD bands for each primer using agarose gel in the two
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muscovy duck ecotypes………………………………………………….. 126
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SYMBOLS AND GLOSSARY
ecology.
Duck day production – the number of eggs laid as a proportion of the number of days a hen
Duck housed production – the number of eggs a hen lays after placement in the laying house
rp – Phenotypic correlation
rG – Genetic correlation
Bp – Base pair
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BH – Body height
Cv – Coefficient of variation
DNTP – 21 deoxyribonucleotide-5-triphophate
DNTP – 21 deoxyadenosin-5-triphophate
DTTP – 21 deoxyguinine-5-triphophate
DF – Degree of freedom
g – gram
h – Hour
HL = Head length
HD – Head width
kg – Kilogram
MS – Mean squares
mg - Milligram
NL – Neck length
n – Sample size
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PROC – SAS procedure
0
C – Degree centigrade
PC – Principle component
P – Probability
se – Standard error
WL – Wing length
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