Beruflich Dokumente
Kultur Dokumente
Elba P. S. Bon
Chemistry Institute
Federal University of Rio de Janeiro - Brazil
elba1996@iq.ufrj.br
Presentation Outline
• Biomass Ethanol
Area
[106 ha]
Ceará: 14,6 106 ha
●
●
Paraíba: 5,7 106 ha
Soya 21,5
Corn 12,3
Harvest
Non mechanized →Straw
Mechanized→
ETHANOL FROM AGROINDUSTRIAL RESIDUE
Straw
and/or
Bagasse
↓
ETHANOL
Sugar Cane Bagasse Composition
• Biomass Ethanol
Enzymatic hydrolysis
of cellulose to
glucose
Cellulose
Hemicellulose
Lignin
Bioconversion of cellulose to ethanol
• Pre-treatment
• Cellulases Production
• Enzymatic Hydrolysis
• Ethanol Fermentation
Enzymes: Endoglucanases e Exoglucanases
Presentation Outline
• Biomass Ethanol
Foreign Collaboration
• LUND UNIVERSITY - Sweden
• The project is open to the establishment of new collaborations.
Main Research Areas
• Development of biomass pre-treatment processes for
sugar cane bagasse and straw
• Raw and pre-treated biomass characterization
• Cellulases / xylanases production
• Enzymatic hydrolysis
• Sugars syrups characterization
• Ethanol fermentation (C6)
• C5 sugars and lignin uses
• Energy optimisation
• Effluents and water
ETHANOL FROM BIOMASS
Bagasse or Straw
Pentoses (hemicellulose)
Pre-treatment
Bio-Refineries/Enzyme Production
Lignin
Enzyme Enzymatic Hydrolysis (Solid Residue)
GLUCOSE
Sucrose Ethanol
Distillation Stillage
Ethanol
Steam Pre-treatment
Steam Pre-treatment
Sugar-cane Bagasse
Aerobic bacteria
Anaerobic bacteria
Fungi
Actinomycetes
Clostridium
Trichoderma
Aspergillus
Studies on Cellulolytic/Xylanolitic Enzymes
BIOETHANOL cellulases
blend
Cellulase production
Enzymes Production and Uses
Industrial application of cellulases
Enzyme blend
Temperature 45ºC
70
60
BIOETHANOL ENZYME
Glucose (g/L)
50
40 COMMERCIAL ENZYME
30
20
10
0
0 20 40 60 80
Time (h)
Sugar Syrups and Lignin
Glucose Syrup
(65 g/L)
Ethanol
fermentation
Residue: Lignin
Treated bagasse
+ BIOETHANOL Enzymes
Burning for Energy
Lignocellulosic hydrolysate
Almeida et. al. (2007). J Chem. Technol. & Biotechnol 82: 340-349
Effects of lignocellulosic inhibitors on the yeast
cells (Saccharomyces cerevisiae)
Almeida et. al. (2007). J Chem. Technol. & Biotechnol 82: 340-349
Ethanol fermentation
Experiments:
Ethanol
quantification
BAGASSE PRODUCTION AND
AVAILABILITY/YEAR (KG)
1 Kg of bagasse: 0,24L
7.700.000.000 Kg of bagasse: 1.849.000.000 L
Enzyme cost contribution
Pilot Plant
BIOREFINERIES
BIOETHANOL RESEARCHERS
Thank You!