4533 Hartford Dr.

Plano, Texas 75093

(972) 599-7071

Objective:
To obtain challenging position that will fully utilize my e
xtensive experience and afford me a chance to gain more knowledge and skills

Supportive Qualifications:
* 25 years working experience in scientific and clinical research.
Performing experiments, preparing presentations and training staff
Strengths:
* Excellent interpersonal and communication skills
* Organized
* Problem solver
* Details oriented
* Team builder

Professional Experience
Present substitute teacher, Richardson, TX
2006-2007 University of Texas South Western Medical Center, Dallas, TX
Internal Medicine-Clinical Research
* Performed measurement of GFR using HPLC for maintenance of heamodyalisis f
or renal failure patients and diabetic nephropathy.
* Measured TGF-B Using Elisa
* Researched methodology and revised protocol
* Organized and stored collected biological samples and lab supplies
analyzed data and prepared
reports using excel
2005-2006 University of Texas South Western Medical Center, Dallas, TX
Internal Medicine-Mineral Metabolism
* Performed analysis of electrolytes such as Na, Cl, K, Mg, Citrate, Amonia
* Collected and stored biological samples
* Entered Data into computer, analyzed it and prepared a report using Word
* Maintained and checked equipments regularly
* Entered data and wrote reports daily
1987-2005 University of Texas South Western Medical Center, Dallas, TX
Internal Medicine - Research Associate
* Acted as liaison between lab members, maintenance staff, Core facilities and o
utside venders and clinics
* Ensured all laboratory procedures preformed in ordinance with safety guideline
s.
* Maintained cryo- cell culture lines with record keeping
* Supervised The tissue culture facility and controlled contamination
* Performed immunohistochemistry, staining and distaining
* Maintained lab Inventory for supplies and equipment with record
* Saved money for the lab through searching for good deals
* Repaired, modified, and maintained electronic and medical equipment used in re
search projects.
* Performed cell culture related molecular biology experiments examples :
* Northern blotting for extracting / analysis of RNA and mRNA.
* PCR technique.
* Radiolabel immunoassays.
* Managed radioactive usage and biohazard waste with record.
* Dissecting Kidney tissues using microtome, and preparing slides for histology
FACS and HPLC
* SDS-PAGE Western blotting for Protein analysis.
* DNA analysis using Southern- blotting.
* Plasmid -vector preparation and bacterial transformation.
* Measurement of Protein/DNA ratio for cell size using fluorimeter.
* Preparing cells for flowcytometric studies
* Prepared the reagents for the department. Organized and ordered lab supplies
and experimental animals.
* Started and managed rodent-breeding program, for our facility to cover the res
earch needs.
* Performed animal surgery techniques on rodent and collected tissue
* Helped principal investigator; in preparing publications.
* Trained New staff and medical students as to techniques and university policie
s
* Met with the government and inspectors for lab needs

1978 - 1987 THEODOR BILHARZ RESEARCH CENTER, Cairo, Egypt

Research Assistant
* Performed Protein analysis; using electrophoretic and isoelectricfocusing te
chniques.
* Extracted flavonoids and heterocyclic compounds using evaporators and chromat
ographic techniques GLC, and elucidated the structure using NMR

EDUCATION
Master's degree in comparative physiology
Cairo, Egypt, 1986
Classes toward a master in Organic Chemistry
Cairo, Egypt, 1982
B.Sc. Chemistry and Biology
Ainshams University, Cairo, 1977