Jude Alfred

83 Grusz Rd (845) 683-1058

Westtown, NY 10998 jad3fb78@westpost.net
SENIOR RESEARCH ASSOCIATE
Molecular Biology / Gene Cloning and Sequencing / HTS / Cell-Based Assay / Cell
Culture/ Molecular Pharmacology
Research professional within a pharmaceutical environment executing experiments
and performing data analysis using various Technical and Scientific tools. Techn
ical and analytical thinker who thrives with projects requiring development and
analysis.
PROFESSIONAL EXPERIENCE
Lundbeck Research USA, Paramus, NJ 2006-2010
SENIOR RESEARCH ASSOCIATE
* Performed radioligand competitive binding assays (dose response, saturation an
d cross reactivity) and functional drug assays (FLIPR, cAMP, GTPgamma S, PI, RIA
, ELISA) on various GPCRs
* Participated in various assays development and optimization (cAMP, PI and GTP
gamma S and Kinase and Immuno Assays)
* Performed MTS and HTS functional screens (Orphan Receptors, mGLURs, NPFF, MCH,
Galanin, HSP90)
* Performed Dissociation and Association Kinetic Assay using FLIPR calcium mobil
ity assay on cells transfected with metabotropic glutamate receptor
* Generated membrane from either transient or stable cells using standard steps
such as centrifugation, sonication and ultra-centrifugation
* Maintained cell line ( 293 human and rat mGLUR5)
* Assessed cell count and viability using Flow Cytometry
* Responsible for primary data management, data analysis, and report generation
using Excel, PowerPoint, GraphPad Prizm, ActivityBase and Spotfire Decision.
Synaptic Pharmaceutical/Lundbeck Research USA, Paramus, NJ 2002-2006
RESEARCH ASSOCIATE
* Assisted in the evaluation and implementation of one new cytotoxicity assay to
support compound profiling efforts within projects.
* Performed cell-based monoamine transporter assay using stably transfected cell
line
* Supported new technologies development (Luciferase, RIA, Discoverex-cAMP, GTPg
amma S)
* Participated in setting up HTS instrumentation, validating and running cell-ba
sed assays using fluorescence technology (FLIPR)
* Responsible for primary data management, data analysis, and report generation
using Excel, PharmWorksop, LSW, PowerPoint, GraphPad Prizm and ActivityBase.
Synaptic Pharmaceutical, Paramus, NJ 2000-2002
LABORATORY ASSISTANT
* Amplified DNA using PCR, gel electrophoresis, DNA extraction and purification,
bacterial (transformation, plating and counting), plasmid vector ligation, rest
riction enzyme analysis, primer (design, synthesis and purification), cDNA libra
ry screening, gene sequence analysis, cell culture and harvest, and transfection
of mammalian cells (CHO and Cos-7 cell line).
Pepsi-Cola Company, Research and Development Department, Valhalla, NY 1999-2000
RESEARCH ASSISTANT
* Assisted in the formulation and processing of new commercial beverages within
a temporary capacity, compounding flavor formulas and modifications, chemical so
lution, ordered raw materials from suppliers, inventory area organization and ge
neral lab maintenance.

Vitex, Inc., New York, NY 1998-1999

ASSISTANT QC COORDINATOR / RESEARCH ASSISTANT
* Performeded viral assay on PK13 and Vero cells with PPV virus, maintained cell
lines, PCR group data analysis and validation, ensuring proper data documentati
on for clinical samples.
* Screened Parvovirus B19 including extraction and purification of DNA using Eas
y-DNA Kit, DNA amplification (PCR) and Gel Electrophoresis (SDS-PAGE)
* Provided input in the writing of procedures and collaborated with QA to establ
ish approved SOPs for QC laboratory
* Implemented and maintained equipment and instrument maintenance and calibratio
n program.

EDUCATION
M.S., PHARMACOLOGY
Sherbrooke University, Fleurimont, Canada
B.A., BIOLOGY
Andrews University, Berrien Springs, MI

SOFTWARE SKILLS
* MS Word, Excel * Activity Base 5.0 * Med-line * Lotus Notes
* MS PowerPoint * GraphPad Prizm * Sequencher * Outlook, 98 NT
* LSW
* Cytosoft 2.1.0 * Spotfire Decision
* BioRails

* Seglab
* GuavaSoft Software * XLFit Software
* FLIPR Tetra Software

EQUIPMENT SKILLS
* 10.0-UNIX-Genetics Computer Group (GCG) * Filtration System (Brandel & Tomtek
) * Expedite 8909 Nucleic Acid Synthesis System
* FLIPR Tetra (Fluorometric Imaging Plate Reader) * Kodak Digital Science 1D So
ftware * Detection instrumentation -Gamma Counter, Beta Counter, DU G40 Spectrop
hotometer
* Biomek (1000, 2000 and FX) * Multidrop 384 System * Centrifuge
* Zymark Rapid Plate-96 * Hamamatsu FDSS * Cytosensor Microphysiometer
* Tecan Genesis RSP 100 * FLEXSTATION * EnVision 2102 Multilabel Reader
* 1450 MicroBeta Trilux * Envision Reader * Embla Cell Washer
* UV/Vis Spectrophotometers * Bio-Rad Gene Pulser * Hydra Microdispenser
* sciCLONE Pipettor System * Transfection Apparatus * Guava Flow Cytometer
PUBLICATION/POSTER
1.Plante GE, Alfred J and Chakir M: The Blood Vessel, linchpin of diabetic lesio
ns, Metabolism 48: 406-409, 1999.
2.Alfred J, Uberti M, Dovlatyan M, Li Z, Smith D, Weiss J, Jubian V, Grenon M, L
i G, Doller D and Brodbeck R: The Synthesis and invitro pharmacology of the sele
ctive mGLUR5 negative allosteric modulator(NAM).
AFFILIATIONS
1. BIOLOGY, Tri Beta National Honor Society, 1994 - present