Simone Haase

2565 Daisy Drive

Loveland, OH 45140
simoneneusshaase@gmail.com
859.652.0249

“… She worked extremely efficiently and was always accurate and diligent … very hard wo
… friendly and helpful … a first-rate team player …†Excerpts from Recommendation
Chemical Engineering, Biochemical Research, R&D / Product Development
Master’s degree in Chemical Engineering, enhanced by international work experience a
nd significant record of accomplishment in research and development. Additional
background in business operations and management. Solutions-driven, perceptive;
quick to learn and apply new techniques. Dependable, quality-oriented team memb
er with strong analytical, organizational and leadership skills. Skilled in prio
ritizing and multi-tasking. Resourceful problem solver; never daunted by a chall
enge. Fully bilingual in English and German; US Permanent Resident. Strong int
erest in combining engineering expertise with responsibilities in one or more of
the following: marketing, product development, product maintenance, sales suppo
rt, budget management, and team leadership. Training and/or experience in:
Molecular Diagnostics, Genetechnology, Plant Transformation, Cloning, Tissue Cul
ture, Cell Culture, Protein Purification, Restriction Digest
Plasmid Construction, Gel Electrophoresis, Regulatory Compliance (cGMP, OSHA, CD
C), Sampling, Qualitative Testing, Quantitative Testing, Statistical Process Con
trol, Policies & Procedures Development, SOP Development, Process Validation, Pr
ocess Transfer, Process Engineering, Control Engineering, Data Analysis, Process
Design, Process Automation, Process Safety
Leadership & Relationship Management – Teaching, coaching, mentoring, leading by exa
mple. Staffing and supervision, employee training and development. Troubleshoo
ting and problem solving. Using active listening techniques, resolving conflict
s by involving all stakeholders in identifying solutions. Motivating diverse in
dividuals to work as a team to achieve business objectives.
Project Management – Needs analysis, system analysis, specification definition, proj
ect plan development, cost estimating, project scheduling, budget control, quali
ty assurance/quality control, failure analysis, root cause analysis, analytical
problem-solving, documentation and reporting.
RESEARCH INTERESTS
Genetechnology, plantibodies, foods, water, oil, biowarfare, enzymatic research,
new instrument development, virology, bacteriology, medical research.
RELATED EXPERIENCE
CINCINNATI CHILDREN’S HOSPITAL MEDICAL CENTER (CCHMC), Cincinnati, OH
2008 – Present
Research Assistant IV, Laboratory for Specialized Clinical Studies (LSCS)
Perform molecular biology research on infectious diseases, primarily rotavirus,
influenza, and cytomegalovirus (CMV) for sponsors such as Merck, GlaxoSmithKline
(GSK), Wyeth, Sanofi Pasteur, Centers for Disease control (CDC), and CCHMC phys
icians. Handle all DNA/RNA testing. Established the genotyping assay currently u
sed in the LSCS; developed multiple assays without assistance. Promoted from Re
search Assistant III, November 2010.
Rotavirus: Currently working with the researchers who developed the vaccine Rot
arix (FDA approved in 2008, sold to GSK). Genotype rotavirus samples to identify
which strains are circulating in various regions of the world. Focus: improving
vaccine efficacy on specific strains. Procedures include RNA extraction; polyme
rase chain reaction (PCR) transcription; DNA replication, multiplication and sam
ple loading.
CMV: Currently working on development of a vaccine to prevent cytomegalovirus in
young girls.
Influenza: Perform research to develop vaccines for annual influenza vaccinatio
ns. Overcame initial challenges to develop an assay for influenza that increased
testing efficiency and contributed to successful vaccine development in anticip
ation of the H1N1 outbreak in 2009. Earned NIH Special Recognition Award for “Extra
ordinary work in H1N1 influenza research.â€
Trained in genotyping at Centers for Disease Control (CDC). Instructed colleagu
es in genotyping methods based on CDC methodologies; developed CCHMC genotyping
procedures. Trained researchers in sterile molecular biology techniques and use
of real time PCR machine (including qualitative and quantitative Real Time PCR)
. Trained associates in preparation of agarose gels, Elisa procedures and more.
Taught nurses how to obtain and package aliquot nasal/swab samples. Participate
d in interview process for new-hires. Trained several new-hires.
Developed standard operating procedures (SOPs) for molecular-based assays to mee
t strict sponsor requirements.
Earned reputation as subject matter expert on molecular biology, assay-oriented
and procedural questions. Hosted CDC visitors during their annual visit. Presen
ted data and results, answered assay-related questions.
Demonstrated strong work ethic, e.g. was hired to replace two full-time people;
managed entire workload without assistance for nearly 2 years, saving at least $
40K in payroll costs. Cross-trained to fill in for others and eliminate downtim
e during employee absences. Partnered with a colleague to start a department-wid
e recycling program Perfect attendance record, 2008-Present.
PLANET BIOTECHNOLOGY, Hayward, CA
04/07 – 09/08
Research Associate
Assisted with research and development of a new, antibody-based therapeutic and
preventative product used to fight anthrax. Worked on team that developed CMG2-
Fc immunoadhesin using transgenic tobacco plants as a production system. Develop
ed 2 proteins: CMG2-Fcg and CMG2-Fcag. One resulted in glycosolated Fc and anot
her that would lead to the expression of a non-glycosolated Fc (anthrax toxin in
hibitors). Gained practical experience in molecular biology techniques, immunoas
says (ELISA, western blot, SDS Pages, and sterile techniques for mammalian cell
culture. Performed plant transformations. Documented all research activities,
participated in weekly meetings, made presentations. Initially hired as Intern.
Following training period, hired as Research Associate and worked independentl
y in all 3 areas – tissue culture, protein purification, and molecular biology depar
tment.
Using genetically modified tobacco plants, developed a new, low-cost antibody th
at worked as a decoy, binding to the anthrax toxin before it was able to replica
te, enter cells and cause damage. Antibody was successfully tested on rabbits e
xposed to anthrax; those which received the antibody survived, those without it
did not. Co-authored a journal article that discusses research methodologies an
d findings: “Recombinant Anthrax Toxin Receptor-Fc Fusion Proteins Produced in Plan
ts Protect Rabbits Against Inhalational Anthrax" published in the Journal of Inf
ectious Diseases and Immunity and in Antimicrobial and Agents and Chemotherapy.
http://aac.asm.org/cgi/content/abstract/AAC.00592-10v1?eaf
UNIVERSITY OF APPLIED SCIENCE AACHEN, Germany
2003 – 2005
Chemistry Tutor
ADDITIONAL EXPERIENCE
Stadtsparkasse Moenchengladbach (SSK MG Bank), Germany
1995 – 2002
Following apprenticeship that provided experience in all aspects of customer ser
vice, was provided with diverse opportunities to learn management and operationa
l aspects of the business. Was promoted to Stock Department; placed stock marke
t orders at the direction of clients. Processed millions of dollars on a daily b
asis. Within 2 years, earned a second promotion. As Financial Analyst, research
ed companies and identified potential investment opportunities. Provided inform
ation that was used by management in making investment decisions. Investigated a
nd resolved errors. Developed reports for presentation by Chief Investments Off
icer to the CEO. Trained new employees.
Completed college one year early; attended school full-time, while working full-
time at the bank.
EDUCATION
Master of Science, Chemical Engineering. Concentration: Biotechnology
August 2006
Ranked in top 5% of class.
Fachhochschule Aachen, Aachen, Germany
Studied Biology and prepared senior research project. GPA 3.87. Dean’s List.
Spring 2006
Thesis: Effect of Antibiotics on Microbial Degradation of the Polybrominated Di
phenylether (PBDE). Hypothesis: Certain antibiotics found in natural environmen
ts inhibit the ability of microorganisms to degrade BFRs.
University of Cincinnati, Cincinnati, OH
TECHNICAL PROFICIENCIES
Instrumentation Skills & Laboratory Procedures: QIAcube, real-time PCR machine,
Thermalcycler PCR machine, Luminex XMap technology (Luminex 200 system), UV/VIS
Spectroscopy, IR Spectroscopy, Gas-Liquid Chromatography (HPLC MS), Reverse-Pha
se Chromatography, Titrations, ICP Mass Spectrometry, Triple-Quadrupole Mass Spe
ctrometry, Atom Absorption Spectrometry (AAS), Enzyme-Linked Immunoassay (ELISA)
, Optical Microscope, Fluorescence Microscope, photometer, Ph meter, autoclave.
Experienced in inventory control of lab chemicals and supplies. Well versed in
lab equipment specification, calibration and maintenance. Experienced in proper
handling, storage and disposal of hazardous materials (HazMat).
Computer Skills: Microsoft Office (Word, Excel, PowerPoint), Softmax (ELISA pla
te reader), Clone Manager, IRS 2.3 (Luminex), Photoshop, Lotus Notes, Internet r
esearch.
ACTIVITIES & COMMUNITY INVOLVEMENT
UNIVERSITY OF CINCINNATI – Captain of the Women’s Alpine (Ski) Race Team. THE MARINE MA
MMAL CENTER – Assisted in caring for injured seals in Sausalito, CA.