EWA WANDZIOCH, PH.D.

7801 Linden Road, Wyndmoor, PA 19038

T: 215-573-5844
Email: ewf76252@westpost.net
C: 215-668-4825
Green Card Holder
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EXPERIENCED SCIENTIST
Multi-skilled scientist with an excellent technical background, strong problem s
olving skills, and an unwavering drive for success. Possesses a proven history o
f designing and implementing experimental procedures in the areas of development
al biology, stem cell biology, and cancer biology. Demonstrated research success
, with publications in top scientific journals and exceptional communication ski
lls to present information as an invited speaker at scientific meetings. Strengt
hs include the ability to effectively work with researchers from a variety of ba
ckgrounds, tap into knowledge of a diverse array of biological tools and techniq
ues, and quickly contribute to achieving scientific goals.
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EXPERIENCE
University of Pennsylvania, Philadelphia, PA 2009-present
RESEARCH ASSOCIATE, DEPARTMENT OF CELL AND DEVELOPMENTAL BIOLOGY
Investigate the generation and function of two different populations of hepatic
progenitors in the liver
and the identification of liver stem cells/cancer stem cells during embryonic de
velopment.
* Discovered new genes marking spatially separated populations of hepatic progen
itors in the embryo through use of laser capture microdissection and microarray.
Generated transgenic mice with BACs for these genes driving tamoxifen-inducible
CRE. Fate-mapped the cell populations from a reporter mouse strain and determin
ed their adult manifestation as two different cell types localized to different
parts of the liver with potentially separate functions in the mature organ.
* Applied scientific knowledge to the in-process development of a method reversi
ng the toxicity of tamoxifen injected into pregnant females to induce CRE recomb
ination.
* Leveraged advanced histological expertise to characterize cells arising from m
edial and lateral hepatic progenitors and develop a novel method of live tissue
slices culture resulting in live cell observation and evaluation of the effects
of toxic agents on cell growth and division.
* Collaborated with colleague in the generation of induced pluripotent cells fro
m normal and cancerous pancreas.
* Utilized technical savvy to develop a method for laser Capture microdissection
of cells from frozen tissues, un-fixed with PFA.
Fox Chase Cancer Center, Philadelphia, PA 2005-2009
POSTDOCTORAL FELLOW, STEM CELL AND EPIGENETIC PROGRAM
Tapped into scientific knowledge to explore the molecular mechanisms leading to
specification of hepatic and pancreatic progenitors from multipotent endoderm in
the mouse embryo.
* Discovered differences in induction of ventral and dorsal pancreas in the mous
e as a result of BMP and TGFbeta signaling. Determined that Tgfbeta inhibition i
n ventral endoderm expands pancreatic progenitors, an important finding relevant
to the generation of functional beta cells from embryonic stem cells. (Wandzioc
h and Zaret, Science, 2009)
* Devised a novel, reproducible in vitro model system recapitulating embryonic d
evelopment consisting of in vitro half embryo culture, applicable for small mole
cule inhibitor/growth factor screening promoting induction/switch of various cel
l fates within embryonic endoderm (Wandzioch and Zaret. Science, 2009).
* Overcame the challenge of a minute amount of cells available for analysis thro
ugh the effective development of a cDNA amplification method suitable for Taqman
RT-PCR analysis of gene expression.
* In collaboration with colleague, discovered that hepatic induction requires FG
F signaling through MAPK pathway (Calmont, et al., Developmental Cell, 2006).
Umea University, Umea, Sweden 1999-2005
PHD STUDENT, UMEA CENTER FOR MOLECULAR MEDICINE
Discovered the function of LIM homeobox gene Lhx2 in hematopoietic stem cell sel
f-renewal and liver development, and made contributions toward the study and pre
vention of hepatic fibrosis and subsequent hepatocarcinoma.
* Generated heart, hematopoietic, neuronal and endoderm cells from embryonic ste
m cells. Optimized biochemical assays to accommodate small amounts of input cell
s; discovered that LIM homeobox gene Lhx2 is responsible for stem cell self-rene
wal in hematopoietic stem cell compartment and prevents activation of stellate c
ells in the liver compartment.
* Determined that Lhx2 knockout mice developed hepatic fibrosis and subsequently
established a mouse
embryonic model for study of the disease.
Umea University, Umea, Sweden 1997-1999
ROTATION STUDENT, DEPARTMENT OF MICROBIOLOGY
Worked on mechanism of oncogenic properties of Oncoprotein 18 highly expressed i
n neuroblastoma and regulated assembly and disassembly of microtubules (Larsson,
et al,. Mol Cell Biol., 1999)

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EDUCATION & AFFILIATIONS
Ph.D. in Molecular Biology - UMEA UNIVERSITY, Umea, Sweden
Doctoral Thesis: Wandzioch, E. (10/12/2004). The role of Lhx2 in the hematopoiet
ic stem cell function, liver development and disease. Dept of Molecular Biology,
Umea University, Sweden
Master in Molecular Biology - UMEA UNIVERSITY, Umea, Sweden
Master's Thesis: Sequence of tumor supressor p53 in mouse lymphoid leukemia cell
line L1210." Frostesjo Nilsson J., Wandzioch E., Heby O. Submitted (FEB-1998) t
o the EMBL/GenBank/DDBJ Databases.
AFFILIATIONS
Society of Developmental Biology
Federation of American Societies of Experimental Biology (FASEB)

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GRANTS & FELLOWSHIPS
Swedish Research Council Postdoctoral Fellowship 2005-2007
Pre-doctoral scholarship from the Swedish Kempe Foundation 2003-2004
Umea University Scholarship 1997-1999
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AWARDS & HONORS
Society for Developmental Biology Award for Participation In Cold Spring Harbor
Meeting 2010
Contributed to the Cover of Science Magazine: Science, 2008 Dec 5; 322 2008
Certificate of Appreciation for Reviewing Abstracts for ABRCMS Conference 20
07
The Pfizer Foundation Award for Participation in the Keystone Meeting on Signali
ng in
Vertebrate Organogenesis 2004
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INVITED SPEAKER
EMBO workshop "Disease, development and stem cells of the pancreas" Karolinska I
nstitute 2010
Department of Cell and Developmental Biology Meeting, University of Pennsylvania
2010
Developmental Biology Club, University of Pennsylvania 2009
Postdoctoral Seminary. FCCC, Philadelphia 2009
EMBO workshop on Liver Regeneration, Max Planck Institute 2005
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TECHNICAL SKILLS
RNAi Ttransfection
In situ Hybridization
Laser Capture Microdissection
Advanced Microscopy Techniques
Immunochisto- & Immunocytochemistry
Cryosections / Paraffin Embedding /Sectioning
Embryonic Stem cells / Adult Stem cells / Induced Pluripotent Cells
Histological Staining
Chromatin Precipitation
Lentivirus Production & Infection
Gene and Protein Expression Analysis
Confocal & Optical Projection Tomography
Advanced In Vitro Mouse Embryo Culture Techniques
Generation, Manipulation, Maintenance and Surgery of Transgenic Mice
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PUBLICATIONS
Wandzioch E, Zaret KS. (2009) Dynamic signaling network for the specification of
embryonic pancreas and liver progenitors. Science., Jun 26; 324(5935):1707-10.C
ommentary: Stacey S. Huppert and Mark A. Magnuson, (2009) New Complexity in Dif
ferentiating Stem Cells Toward Hepatic and Pancreatic Fates, Science Signaling 2
(83), pe50.
Gadue P, Gouon-Evans V, Cheng X, Wandzioch E, Zaret KS, Grompe M, Streeter PR, K
eller GM. (2009) Generation of monoclonal antibodies specific for cell surface m
olecules expressed on early mouse endoderm. Stem Cells., Sep 27(9):2103-13.
Zaret KS, Watts J, Xu J, Wandzioch E, Smale ST, Sekiya T. (2008) Pioneer Factors
, Genetic Competence, and Inductive Signaling: Programming Liver and Pancreas Pr
ogenitors from the Endoderm. Cold Spring Harb Symp Quant Biol., 73:119-26.
Calmont, A.B., Wandzioch, E., Tremblay, K.D., Minowada, G., Kaestner, K.H., Mart
in, G.M., Zaret, K.S. (2006) An FGF-response pathway that mediates hepatic gene
induction in embryonic endoderm cells. Developmental Cell., 11, 339-348.
Wandzioch, E., Kolterud, A., Jacobsson, M., Friedman, S.L., Carlsson, L. (2004)
Lhx2-mice develop liver fibrosis. Proc Nat. Acad. Sci USA. ,101(47):16549-16554.
Wandzioch, E., Edling, C.E., Palmer, R.H., Carlsson, L., Hallberg, B. (2004) Act
ivation of the MAP kinase pathway by c-Kit is PI-3 kinase dependent in hematopoi
etic progenitor/stem cell lines. Blood.,104(1):51-57.
Kolterud, A., Wandzioch, E., Falieeva, L., Carlsson, L. (2004) Lhx2 is expressed
in the septum transversum mesenchyme that becomes an integral part of the liver
and the formation of these cells is independent of functional Lhx2. Gene Expres
s. Patterns., 4(5):521-528.
Carlsson, L., Wandzioch, E., Pinto do O, P., Kolterud, A. (2003) Establishment o
f multipotent hematopoietic progenitor cell lines from ES cells differentiated i
n vitro. Methods Enzymol., 365:202-214.
Pinto do O, P.*, Wandzioch, E.*, Kolterud, A., Carlsson, L. (2001) Multipotent h
ematopoietic progenitor cells immortalized by Lhx2 self-renew by a cell nonauton
omous mechanism. Exp Hematol., 29(8):1019-1028. (*equal contribution)
Larsson, N., Segerman, B., Gradin, H.M., Wandzioch, E., Cassimeris, L., Gullberg
, M. (1999) Mutations of oncoprotein 18/stathmin identify tubulin-directed regul
atory activities distinct from tubulin association. Mol Cell Biol., 19(3):2242-2
250.