Title: Leukocyte observation Date: 20/04/2011

Name: Do Le Tan Lab Partners:

Introduction: In the nature, almost the cell in side is transparent and need to be dyed to observe. Cytochemistry is the method used for the chemical nature of the cell, and it will be used to observer many kind of cells in our blood such as: Red Blood Cells : Erythrocytes,or red blood cells,are biconcave disks.They transport the oxygen used in aerobic respiration and carry away some carbon dioxide wastes.When oxygen diffuses into blood,it binds with hemoglobin,an ironcontaining pigment that gives red blood cells their colour. White Blood Cells Leucocytes,or white blood cells,function in daily housekeeping or defense.They differ in size,nuclear shape and staining traits.There are five types: neutrophils, eosinophils, basophils, monocytes and lymphocytes. In this experiment, only monocytes and lymphocyte are observed Monocytes: are large mononuclear cells.This nucleus is quite large,sometimes round,but more usually irregular.The monocytes differ from lymphocytes in that they can spread on a glass surface. Lymphocytes: nucleus is generally centrally placed and round but it may be oval or slightly concave.The cytoplasm is basophilic and shows mitochondria,together with a few azurophile granulations and a centrosome which is particularly clearly defined. Materials: 1. Drops of animal blood (except poultry) 2. Slides 3. Giemsa stain solution 4. Ethanol 5. Water Methods: 1. Smear specimen preparation. 2. Drop a small drop of blood on the slide and spread it lightly and rapidly. 3. Air dry. 4. Fix slide in ethanol for 5 minutes. 5. Air dry. 6. Place Giemsa stains solution for 2 minutes; try to shake it gently during incubation. 7. Rinse slide with water. 8. Air dry. Data:

Image 1: There is 1 lymphocyte on the right which has pink color and has the nucleus inside fill nearly all the cell. There is 1 monocyte on the left which has also pink color by giemsa but the cell has nucleus inside does not fill cell

Image 2: There is another image of monocyte which is the pink one Discussion: The blood smear should be as thin as possible to be able to observe cells clearly and not to have too much of them.

Tap water will not contaminate the blood because before rinsing with water it is fixed. By fixation treatment, the general morphology and internal structures are preserved. Write the name of the experiment on the slide after dropping blood and spread it to avoid negligence Control slide is also used in this experiment. It is better to set paper under the slide when place giemsa solution to the slide Conclusion: