Code No: R05222303 Set No.

1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Represent a melting curve of a double helix DNA and explain it in detail? [16]

2. Write a note on the famous Messelson & Stahl experiment on proving semi conser-
vative mode of DNA replication. [16]

3. What is the mechanism & importance of “proof reading” function of DNA poly-
merase in DNA replication? [16]

4. With respect to tRNA structure, explain:

(a) Stems& loops

(b) Anticodon arm. [2 × 8 = 16]

5. Which are the different types of rRNA associated with eukaryotic ribosome? How
are these synthesized? [16]

6. How are Wobble hypothesis and the genetic code correlated with each other? [16]

7. How is the process of eukaryotic process of translation different from prokaryotic

process. [16]

8. With context to mutations, what is meant by: [2 × 8 = 16]

(a) Base substitutions

(b) Insertions and deletions.

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Code No: R05222303 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Which form of DNA exists as the natural form present in living cells? Explain in
detail. [16]

2. What is meant by “rolling circle” form of DNA replication? Give an example. [16]

3. What is meant by ‘end replication problem’ in DNA synthesis? Discuss the role of
telomase in it. [16]

4. How are eukaryotic and prokaryotic RNA polymerases different from each other?
[16]

5. What is the relevance of intron and exon sequences with respect to the final mRNA
formation in eukaryotic posttranscriptional processing? [16]

6. Explain how the genetic information present in the mRNA is decoded into amino
acid sequence during the process of translation? [16]

7. What are post transnational modifications? Give some examples. [16]

8. Briefly explain the different DNA repair mechanisms existing in a cell. [16]

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Code No: R05222303 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. what is the primary structure of DNA? Explain in detail. [16]

2. Write a note on the famous Messelson & Stahl experiment on proving semi conser-
vative mode of DNA replication. [16]

3. Discuss about the various enzymes involved in carrying out the process of the DNA
replication. [16]

4. Discuss about the different types of RNA and the RNA Polymerases, which are
involved in synthesizing them. [16]

5. Write a note on how tRNA is produced by processing larger pre-tRNA transcript.

[16]

6. How are Wobble hypothesis and the genetic code correlated with each other? [16]

7. Explain how conjugation is a process through which genetic recombination is pos-

sible in bacteria? [16]

8. With reference to acting as mutagens, explain: [2 × 8 = 16]

(a) Uv irradiation
(b) Ionizing radiation.

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Code No: R05222303 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What are the main functions of DNA in a living cell? [16]

2. How does DNA replication occur in Mitochondria DNA? [16]

3. What are Telomers in DNA? How do they replicate. [16]

4. Write notes on:

(a) Prokaryotic RNA polymarase

(b) Eukaryotic RNA polymarase. [2 × 8 = 16]

5. Explain the sequence by which introns are removed from a eukaryotic pre-mRNA.
[16]

6. Elaborate the statement “Genetic code is a triplet, redundant, nonoverlapping &

comma free code”. [16]

7. Write short notes on: [2 × 8 = 16]

(a) tRNA as an adaptor molecule

(b) Difference between prokaryotic and eukaryotic ribosomes.

8. With reference to acting as mutagens, explain: [2 × 8 = 16]

(a) Uv irradiation
(b) Ionizing radiation.

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