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Research International Development Economic & Social Research Advanced Technology Energy Research Environmental Research Services Laboratory & Chemistry Services RTI NewsroomNews Releases Expert Sources Ongoing Projects RTI in the News Image Gallery Events Calendar Subscribe to RTI News Media Contacts Corporate Facts Published ResearchRTI Press Publications Bulletin CareersJob Openings Internships & Fellowships Benefits Frequently Asked Questions Corporate Culture RTI Fellow Program Senior Management Annual Reports Contact RTI Recruitment Services Working with RTIGSA Schedules Contract Vehicles PO Terms Current RFP List Supplier Diversity Partner Link-Up System Commercialization & Commercial Services Subcontract Terms Published Research RTI Press About the Press Executive Committee and Editorial Board Publications Bulletin Published Research Apoptotic anticancer effect of alvaradoin E isolated from Alvaradoa haitiensis Mi, Q.W., Lantvit, D., Reyes-Lim, E., Chai, H., Phifer, S.S., Wani, M.C., & et a l. (2005). -------------------------------------------------------------------------------Full Citation: Mi, Q.W., Lantvit, D., Reyes-Lim, E., Chai, H., Phifer, S.S., Wan i, M.C., & et al. (2005). Apoptotic anticancer effect of alvaradoin E isolated f rom Alvaradoa haitiensis. Anticancer Research, 25 (2A):779-787. -------------------------------------------------------------------------------Other Publications by: Sharnelle S. Phifer

Mansukh C. Wani Monroe E. Wall Abstract Two anthracenone C-glycosides, alvaradoins E and F, isolated from the leaves of Alvaradoa haitiensis Urb. (Simaroubaceae), were found to have potent inhibitory activities with cultured cancer cells. Using the in vivo hollow fiber model, the se compounds demonstrated significant growth inhibition at the i.p. site when te sted with KB, LNCaP, and Colt cells. To determine if these anthracenone C-glycos ides mediated anticancer activity through an apoptotic pathway, a series of assa ys were performed with the IOS isomeric compound, alvaradoin E. With a DAPI assa y, treatment of LNCaP cells with alvaradoin E at concentrations of 0.4, 2, 10, o r 50,mu M for 24 or 48 h showed chromatin condensation, a morphological characte ristic of apoptosis. Mitochondrial membrane potential, analyzed with a DiOC(6) u ptake assay, showed that treatment of LNCaP cells with 0.07, 0.14, 0.28, 0.56, 0 .86, and 1.12 mu M alvaradoin E for 12 h caused dose-dependent membrane depolari zation, another indication of early apoptosis. Also, with an annexin V-FITC assa y system, treatment of HL-60 cells with 0.07 mu M alvaradoin E for 24 h increase d annexin V-FITC binding from 3 to 25.9% (8.6 fold). Finally, with the TUNEL ass ay system, treatment of HL-60 cells with 1.12,M alvaradoin E for 32 h increased FITC-dUTP binding from 1.2 to 12.1% (10 fold). These data suggest alvaradoin E i s an effective anticancer agent that induces apoptosis. Additional studies to es tablish clinical utility should be of interest Site Map Contact Us Subscribe Terms & Conditions Privacy Policy Access ibility Standards Version 2011 RTI International. RTI International is a trade name of Research Triangle In stitute.