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Parts of a Compound Microscope

Here is a quick overview of the most important parts of a compound microscope (biological microscope) and their function.

The following list of terms can also be found in the glossary: Condenser: This is a system of different lens elements which is mounted beneath the stage of the microscope. It contains an iris diaphragm which controls the diameter of the light beam. The light beam should be adjusted to be larger or equal to the numerical aperture of the objective in use. Condensers can be moved up and down. The normal operating position is up. Base: This is the bottom part of the microscope, it contains the lamp. Coarse Focus: Also referred to as rough focus, this knob raises and lowers the microscope stage quickly. It should only be used in connection with the low magnification lenses. Eyepiece Lens: Also known as ocular lenses, they magnify the image of the objective. The eyepiece is the lens into which a person looks into when observing. The total magnification of a microscope is calculated by multiplying the magnification of the objective by the magnification of the eyepiece. Many eyepiece lenses have a magnification of 10x ot 15x. Fine Focus: This focus knob moves the stage up and down in small steps. It is used to focus at different layers of the specimens. Head: This is the top part of the microscope. It carries the eyepiece(s) and other optical elements. There are several different types of heads: a monocular head is designed to carry only one eyepiece, a binocular head carries two (but does not give stereoscopic vision in compound microscopes) and a trinocular head is designed to carry a camera as well. Mechanical Stage: This type of stage is equipped with a slide holder and two knobs to turn. One knob moves the stage backwards and forwards, the other one moves the slide sideways. Nosepiece (or revolving nosepiece, turret): This part carries the objectives. It can be rotated. Objective Lens: This is a highly magnifying lens system, it is located close to the specimen to be observed. The image of the objective is then magnified again by the ocular lens which is close to the eye. Stage: This is the flat surface on which the slides are placed on. It can be moved up and down for focusing. Stage Clips: These are clips that hold the slide. Trinocular Head: This microscope head has three exits, two for viewing (for binocular vision) and a third exit to connect a camera. Some microscopes also allow for taking photographs through a special adapter at the eyepiece, but a trinocular head offers more stability and is to be preferred for photographic work.

Compound Microscope Parts and Functions


Wannabe microbiologist looking for compound microscope parts and functions? This widely used instrument in the field of life sciences helps solve many mysteries of life. The following article will cover information on compound microscope parts and functions. One of my most memorable day of college life was walking into the microbiology lab and placing a bacteria smear on the glass slide. It was a rush to be the first to stain the slide with the crystal violet dye for two minutes and then washing it off with iodine. Once the decolorizing was carried out for just 2 to 3 seconds, we made a beeline to flood the slide with safranin counter stain. After two minutes, we washed off the slide with water and blot the excess water dry. Then passed the slide over the Bunsen flame, to stick the bacterial smear over the slide. You must be wondering why was I so excited to carry out bacterial staining? Well, it was not the staining that excited me, but the opportunity to open my eyes to the invisible world of microorganisms. I wanted to use the compound microscope that would give me a glimpse into the world of the tiny, invisible bacteria. I was bored of the long theory lectures on the compound microscope parts and functions. I was waiting eagerly to use the weapon of a microbiologist, "the microscope" as soon as possible. With all the exhilaration and hullabaloo in my mind, I did not pay much attention to the very important lecture on compound microscope parts and functions. The staining was perfect, the only thing that went wrong was that I was unable to give satisfactory answers to the questions related to the microscope parts and functions, when asked by the lecturer. I am sure many of you must be looking for answers about compound microscope parts and compound microscope functions, for a project or understand the microscope in detail. This article will surely help you learn more about the compound microscope parts and functions. Parts of a Compound Microscope A compound microscope helps in magnifying an image in two stages. It uses an objective lens that has many different powers on a turret and an eyepiece that helps in magnifying the image formed by the objective lens. The compound microscope parts are basically divided into the structural parts of a compound microscope and the optical parts of a compound microscope. A microbiologist should know both these parts of a compound microscope well, before he uses the weapon to enter the invisible world. You can read more in detail on compound microscope basics and uses. Labeled Compound Microscope Diagram The following labeled compound microscope diagram will help you identify the various compound microscope parts and functions. Structural Parts of a Compound Microscope The compound microscope parts are divided into three basic structural components, which can be explained as follows:

Head: The head or body of a compound microscope contains the optical parts of the microscope. Base: The base of a compound microscope is helps in supporting the microscope and also contains the illuminator. Arm: The arm acts as a connector between the base the head of the compound microscope.

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Optical Parts of a Compound Microscope

Eyepiece: The eyepiece is the ocular lens that helps you look through to see a magnified image from the top of the microscope. The lens have a power of magnification of about 10x or 15x. Eyepiece Tube: The part that connects the eyepiece with the objective lens is the tube. Objective Lens: You can see three or four objective lens attached to the end of the tube. The lenses range from 4x to 100x magnifying powers. To make matters simple, you can identify the longest objective lens as the one that provides the highest magnification power. The shortest objective lens is the one that provides minimum magnification power. Turret: The nose piece that supports the objective lens is known as turret. You can rotate the turret and change the power magnifications as per requirement. Coarse and Fine Focus: These are the knobs that help focus the microscope. There are many compound microscopes that have coaxial knobs. The coaxial knobs are build on the same axis as the fine focus knob on the outside. This proves to be more convenient to use as you do not need to fumble with different knobs. Stage: The stage is the flat surface on which you keep the specimen to be observed. Stage Clips: The stage clips are used when you need to move the slide to view different sections of the specimen. Aperture: The tiny hole in the stage that helps in transmitting base light to the stage. Illuminator: The light source that is located at the base of the microscope. Many light microscopes use low voltage halogen bulbs. Th

Shaneley A. Aonuevo VI Virgo Science & Health - Biological Microscope Parts and their Functions The parts of the Biological/Compound Microscope * Eyepiece *Body tube *Course adjustment *Revolving Nosepiece *Objectives *Fine adjustment *Stage *Clip *Diaphragm *Mirror *Base *Arm

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