Beruflich Dokumente
Kultur Dokumente
Experimental Investigation”
Submitted by
Ram Kumar
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CERTIFICATE
Hons. project.
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Acknowledgement
At the outset I acknowledge the constant support and encouragement from the Principal Dr.
Savithri Singh who has been a source of inspiration to undertake any unsolved scientific
puzzle having evolutionary meaning. I am also grateful to her for providing facilities which
I express my deepest gratitude Dr. Ram Kumar, Department of Zoology, Acharya Narendra
Dev College, University of Delhi, under whose guidance and supervision this work has been
completed. I am thankful to him for being extremely kind and generous in giving me the
opportunity to work with him. I am highly indebted to him for his support and constant
encouragement at all times. His in-depth knowledge, tactful criticism, dedication to work and
I also thank Victor R. Alekseev, Professor, Dr., Head of Department Taxonomy and
Scientists.
I am thankful to all faculty members of the Zoology Department of Acharya Narendra Dev
College who has been constantly encouraging and supporting the project work regardless of
I would also thank to Mr Amit K. Awasthi, Senior Research Fellow at the ecosystem
research lab, Acharya Narendra Dev College for his help and encouragement.
I sincerely thanks to the staffs of Zoology Deperatment, Acharya Narendra Dev College, who
supported me throughout this project. I acknowledge the assistance provided by Mr. Tara
Debadityo Sinha
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CONTENTS
Abstract .…….……………….…… 6
Result ……………………………..28
References .….…………………………35
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Abstract:
The existence and survival of a species depend upon its adaptation to the
catastrophic periods of their life cycle through millions of years of adaptation. Most
of the zooplankton taxa form resting stages which get accumulated in the sediment
of the water body. The accumulation of resting stages in bottom sediments forms a
reservoir of both the past and recent species composition in a particular water body
eutrophication and pollution etc.) hence also termed as egg bank for animals and
seed bank for plants. Therefore resting stage egg bank constitute an ecological
particular water body. Resting eggs of planktonic organisms from sediment are
As rotifers, cladocerans and copepods produce resting stages which can remain
viable for many decades, they have been now an important tool of ‘resurrection
ecology’.
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Herein, I studied the zooplankton diversity in the sediment egg banks of two lakes:
Lake Bhalaswa and lake differing in trophic status, anthropogenic pressure and
draught condition. Bottom sediments from both the lakes were collected
sediment were identified to the lowest possible taxa. Observations were done at
collected from TAJPUR PAHADI LAKE whereas in the sediment collected from
obtained in my study indicate that the bottom sediment is evolutionary archive of the
zooplankton community. My study also indicates that the bottom sediment may be
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Introduction:
Many lakes and ponds are subjected to eutrophication, draught (e.g. Lake
Bhalaswa, Lake Badkhal) and heavy anthropogenic pressure (Tajpur Pahadi lake).
Consequently most water bodies are becoming temporary habitats for fresh water
communities. Temporary waterbodies are widespread in tropical regions and in
recent years the abundance and ecological importance of these ephemeral
waterbodies have drawn attention ecologically (Williams, 1987). These habitats are
transient for the aquatic organisms, shifting from inhabitable to uninhabitable, and
then back to inhabitable. Aquatic organisms for continual existence must either
disperse to other habitats (forming spatial metapopulations) or remain dormant for
the inhabitable period. The most work done in aquatic ecology have been directed to
permanent (ie. Hydroperiod> 1 year) lentic and lotic waters. The lack of attention
may be due to the fact that temporary waters are often too small for fishing,
recreation or holding water for agricultural or other uses. Without obvious utility to
man they have been largely ignored. Without obvious utility to man they have been
largely ignored, hence having less basis for the protection of these unique and
endangered habitats (Kumar & Prasad, 2004). Concern is expressed for the state of
the art of temporary aquatic habitats, as our exploitation and destruction of such
habitats continue globally. Habitat destruction is a primary threat to invertebrates in
general, and in particular invertebrates with specific requirements (Collins,
1991).Keeping in view the conservation biology of aquatic organisms, the study of
such habitats is essential (Kumar & Prasad, 2005). In order to understand the
adaptation and survival strategies of zooplankton it is important to understand their
life history attributes.
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Reproduction and Life Histories of Zooplankton:
produce young without mating) throughout much of the growing season. During
periods of stress (low, food, light, temp., crowding) meiosis occurs leading to the
The haploid males and females mate and the fertilized, diploid eggs are produced
which are encased in a heavy shell or ephippium. These resting eggs are shed
when the female molts and over winter in the sediments and will develop into amictic
diploid females
These resting eggs are very durable and can remain a viable “seed bank” of
number of rapid instar stages which differ mainly in size. Temperature is the main
factor that determines growth rate. A Daphnia gives birth to her first brood in about
Copepods are bisexual and carry their fertilized eggs in external egg sacs. As with
much longer than compared to Rotifers and Cladocerans. The degree to which a
copepod population enters diapause, the instar stage when diapause occurs and the
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Fig. 3. Schematic diagram showing dry and hydroperiod of a tropical temporary
aquatic habitat and survival strategies of the inhabitants. ( Kumar & Prasad, 2004).
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Resting Eggs:
resting eggs or diapause stages (Cáeceres, 1997; Brendonck et al., 1998a). This
suitable conditions persists again, but in diapause the organism does not
resume its development until its diapause is broken, even on the arrival of
suitable conditions. The conditions which are necessary for breaking the diapause
depend mostly on the species to which the egg belongs and often vary even among
populations of same species (Wyngaard, 1988). Generally the eggs either settle at
the bottom of lakes sediment or remain freely floating. Photoperiodism and light
intensity are the main stimulus. With time, some eggs hatch but most remain
unhatched, and they accumulate and contribute to the existing eggs and forms the
“Egg Bank”. Egg banks are a common feature in variable water systems such as
shallow lakes, ephemeral pools (de Stasio, 1989). After the ephemeral pools get
filled by rain some of them float and hatch and remaining remain as egg bank in the
sediment.
Most of the zooplankton taxa form resting stages which get accumulated in
the sediment of the water body. In a particular water body, this forms a reservoir of
both the earlier and recent species composition. As resting eggs in the sediment can
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remain viable for decades or centuries, the longlived dormant stages of zooplankton
additionally offer a unique source of living historical information (eg. Weider et al.,
Hairston et al., 1999a,b; Kerfoot et al., 1999; Cousyn et al., 2001; Limburg & Weider,
2002), the study of which was denoted as resurrection ecology by Kerfoot et al.
(1999). Resting stage egg bank thus constitute an ecological evolutionary reservoir
Meester, 2003).
All of the zooplankton species do not produce resting eggs or dormant stages
and those produced do not essentially survive for long (Hairston, 1996). The
zooplankton known to produce resting eggs or dormant stages are the rotifers (eg.
Cereidaphnia).
In monogonont rotifers, the egg has yolk and the embryo get surrounded by a
thick multilayered shell. The eggs are spherical and get sink in the sediment. The
often encased in a transparent gelatinous cyst with an outer layer of mud particles
(Fryer & Smyly, 1954; Smyly, 1961, 1967; Elgmork, 1962). In calanoid copepods,
resting eggs are spherical and sink freely to the bottom after deposition (Viitasalo &
Katajisto, 1994).
In cladocerans, the daphnid eggs are well known for their structure. Here, 1 or
2 resting eggs are encapsulated by the carapace valves and forms a structure called
“ephippium” which is shed at molting. The ephippium get attached to the floating
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debris or algae and thereafter sink to the bottom after a brief floating phase
Large pools of dormant stages exist in the active egg bank in all groups of
large branchiopods (Hildrew, 1985; Thiéry, 1997; Brendonck & Riddoch, 2000b),
calanoid copepods (de Stasio, 1989; Hairston et al.; 2000), cyclopoid copepods
(Hairston et al., 2000), and cladocerans (Lampert & Krause, 1976; Smyly, 1977;
Herzig, 1985; Carvalho & Wolf, 1989; review in Hairston, 1996; Cáeceres, 1998b;
Cousyn & de Meester, 1998; Hairston et al., 2000). Hatching is mainly restricted to
the resting eggs in the upper 2 cm of the sediment (Herzig, 1985) that is indicated
as the ‘active egg bank’ according to Cáeceres & Hairston, (1998). The largest
fraction of viable eggs occurs in the top 4-6 cm of the sediment (Herzig, 1985;
Carvalho & Wolf, 1989; Hairston & van Brunt, 1994; Cáceres, 1998b).
Fryer, 1996) and temperature have been shown to be important for inducing or
breaking diapause. In deep lakes, temperature and the gaseous state (Fryer, 1996)
osmotic and oxygen values during an inundation cycle are the most important
indicators of the suitability for growth and reproduction (Brendonck, 1996). In rotifers,
cladocerans and copepods, most hatching takes place in spring (Herzig, 1985; Wolf
& Carvalho, 1989; de Stasio, 1989, 1990; Cáceres, 1998a, b; Hairston et al., 2000).
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The stimulus that triggers the production of diapausing eggs in the above
density, or food (Hutchinson, 1967; Stross, 1969; Kleiven et al., 1992; Gilbert, 1995;
Hairston & Kearns, 1995; Piercey & Maly, 2000; Alekseev & Lampert, 2001).
Eutrophication also plays an important role in increased resting egg production as,
(Kerfoot et al., 1999). Also loss of drying may also result in loss of biodiversity within
• The stored egg bank acts as buffer against years when reproduction is low or
can maintain genetic variability of the population and affect the rate of
evolution. (Templeton and Levin 1979; Coleman 1983, Hairston and De Statio
1988).
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• In spite of their differences in phylogeny and organism size, the zooplankton
egg bank and aquatic plant seed bank exhibit similar characteristics that allow
Overall, the egg bank plays a critical role in allowing species living in variable
habitats to adapt to the changing environment in which they live. However except
two published papers (Kumar and Prasad 2004 a, b) I did not get any information on
egg bank or seed bank diversity in the sediment. This prompted to me to study the
egg bank diversity in the sediments of two lakes of Delhi differing in trophic status,
The primary aim of my project was to carry out a short duration study to elucidate
species richness and time taken to hatch from the sediment of the two lakes and to
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Materials and Methods:
Study lakes:
The Lakes chosen were Tajpur Pahadi Lake, Badarpur and Bhalaswa Lake,
Bhalaswa.
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DESCRIPTION OF THE TAJPUR PAHADI LAKE
The area is under “New Delhi Municipal Corporation”. The place was actually a
stone quarry from which it derived its name ‘Khaan’. The area situated just beside
the “Badarpur Thermal Power Station” was used extensively for extracting stones
until the underground water came out. The area was then left as it was since 70’s.
Gradually the place turned into a very deep lake with accumulation of more rain
water underground and some local sewage. The total area of the place is
approximately 30 acres. With time the lake became a habitat of many aquatic
animals like waterfowls, damselflies, fishes etc. Till now no-one had studied the
waterbody and there is not single written information about its biodiversity. The lake
is now at verge of vanishing because of developmental projects undertaken by as
the Municipal corporation. The water is being extracted land filling is being done for
the last 2 years. The lake is now surviving through several small shallow ponds and
puddles which are the only niche to the aquatic animals which once used to occur in
the lake. It is suspected that the lake will be permanently extinct within few months.
Water criteria:
Water color- straw color
Highly turbid (secchi disc measuremt 30 cm)
Phosphate level: > 600 μgL-1
Nitrate level: >800 μgL-1
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Fig.2. Road map of Tajpur Lake ( source:http// www. mapmyindia.com)
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DESCRIPTION OF THE BHALASWA LAKE
Common Name: “Bhalaswa Jheel /Bhalaswa Recreational & Sports Complex” (Fig.
3)
Pin: 110043
The lake is used for water sports activity. The lake unlike the Tajpur Pahadi
Lake is a permanent and very old lake. The water is not as eutrophic as in the Tajpur
Pahadi Lake. The lake is under Delhi Development Authority and recently it was
declared Recreational Complex by the Delhi Govt. in 2003. The lake is to be used
Water criteria:
Water color- Clean / light green
Turbidity (secchi disc measuremt 80 cm)
Phosphate level: > 300 μgL-1
Nitrate level: >500 μgL-1
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Fig.3. Road map of Bhalaswa Lake ( source:http// www. mapmyindia.com)
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A
Table : Comparison of the two lakes with respect to their current status
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WATER Lake is being landfilled Water level in the lake is
SOURCE
by the MCD being maintained by the
DDA
A stone quarry turned to
Sample collection:
We followed same methods for collecting the soil samples. The sediments were collected
from the site with the help of a PVC sediment corer of diameter 3.08 cm or 2 inches and
length 27 cm. the corer was pushed vertically upto 4 cm in the lake bottom and then taken
out with blocking its mouth. The soil samples were taken from 4 randomly selected places
within the lake. Soil samples were packed in plastic bags and covered with aluminium foils
Zooplankton in the water column was filtered through a plankton net with 53
micrometer mesh size. Photographs of the sediment and water sample collection have
been shown in Fig. 9-13. Sediment samples so collected were brought to the laboratory sun
dried and stored at 4 degree centigrade temperature till the hatching experiment was
conducted.
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Fig. (4-8). Site pictures of the Tajpur Pahadi Lake
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Fig. 9 . Sediment collection from the Tajpur Pahari Khaan.
Fig. 10 . Collection of zooplankton from the water column in the Tajpur Pahari
Khaan.
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Processing of the samples:
The wet soil samples were then allowed to sundry for 4 consecutive days. After the
soil samples were dried completely, ground them into powder form with hand so as
to protect the eggs from any mechanical pressure. The samples were then mixed
thoroughly into a single mixture. From this sample, 4 subsamples were taken
weighing 50g each. Then each subsamples were incubated with autoclaved tap-
water in a 250 ml beaker in ambient temperature, the water depth in the beaker was
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maintained 5 cm. The samples were then observed everyday until asymptote was
reached. The water was decanted in a petriplate thropugh a 30µm mesh and
were then taken out and allowed to grow in a separate beaker containing Chlorella
vulgaris till it become adult enough to be identified. The animal was then preserved
with 5% formalin. Then it was identified with the help of identifion keys.
The identification keys consulted for this study were “Koste, (1978);
Edmondson (1959)” for the rotifers and “Michael and Sharma, 1988; Edmondson
RESULTS:
As the project aims at comparing only the number of species that had emerged from
the sediment egg bank of the zooplankton, we had ignored any measurement of
density or embryo number. As the lakes had different history- Tajpur lake was a
newer one (less than 50 year) and formed due to ground and sewage water, the
water was eutrophic as compared to the Bhalaswa lake which is a natural and and
very old lake. The number of species emerged from the sediment of the Tajpur lake
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was sooner ( 3rd day of incubation) than that of the Bhalaswa lake (6 th day of
incubation); and also the number of species of cladocerans which emerged from the
egg banks were more in the case of Tajpur pahadi lake as compared to Bhalaswa
lake. The reason may be due to the highly eutrophic conditions which induce
al., 1999) in the case of the Tajpur pahadi lake. In either of the lakes the diversity of
zooplankton was higher in the sediment egg bank than that in the water column. A
the sediment collected from the Tajpur Pahadi lake whereas in the sediment
4; Ostracoda: 1)
Table : Zooplankton species emerged from the sediment collected from Tajpur
Pahadi Lake
DAYS
1
2
3
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6
10
11
12
15
90
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Table : Zooplankton species emerged from the sediment collected from Bhalaswa
Lake
DAY
1
2
3
7
8
9
10
11
12
13
14
15
20
45
59
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Resting Egg Of Daphnia An Ostracod hatched
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Pleuroxus aduncus hatched
My short term study also proves that the lake sediments act as an evolutionary
archive of the diversity of the zooplankton. Zooplankton in the past and their history
may be constructed with the help of resting stages analyses. Resting stages do not
only help inh survival under unfavorable condition but also act in reducing
competition and over crowding in lakes and ponds by dispersal of egg bank. Resting
stages has applied aspects also which can be explained under following sub
headings:
AQUACULTURE:
Today rotifers serve as the only food offered for larvae of many species of marine
and fresh water fish. Rotifers act as first food during the early developmental stages
culture however resting stages may act as buffer food which may be used during
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developmental stages of fish larvae. It has been reported and also verified that
40,000-1,00,000 rotifers are required for raising one fish larva to age of 30-34 days
But the main problem with rotifers are their storage and preservation.
Therefore, the ability of producing resting eggs can be used to preserve them
RESSURECTION ECOLOGY:
Many of the resting stages are very resistant and may survive extreme
temperatures, high pressure, or drought and remain viable for several decades. This
body.
Identifying the resting stages can assess species diversity, where possible on the
archives of past genetic changes involves the integration of different techniques and
tools and experimental quantitative genetics (Hairston et al., 1999; Cousyn et al.,
2001).
RESEARCH:
It is still unclear what specific factors control the duration of dormancy for these
species what are triggering factors of production of resting stages and what are the
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optimum condition of emergence of animals from restring stages. Therefore question
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