A T.

the siowest up the Which plant pigment is least soluble Qr moves

"'ci"ia'ii,iiu

5.

V v:Do,,=t. ,t ' tv er rr- ercr r' *ttw v''r ,., ru, rFl? .tr44r sctrvent and traveled the farthest up tire
c

stn;--r

of paper? Why?

stationary Phase? WhY? )ca Mosr iguuplt.fr sv8qrlNcp ,

6.

phase?

:9SNT-' the tast up the stationary slowly while others -anro ffin\/A clnrvlvffi6 .th"* traveled very fast h.Fracrrf an\t./.r<gf,trotl9 INP SOt

lx litq

-. -

"

7. @lnotnatograp",

" - lT ls

rJsr '2 ,p

'<r^' i*tn'tf

,L+

a ),*ercise 23 Starch as an \ t/\

manufacture their food

Indicator of Photosynthesis
the form of simpie
sr.rgar phosphates.

Photosyntiresis allovrs piant-s tc chlorophyll is an essential factor in photosynthesis' organic moiecules :;e

i'

These

LU einer

a.

transported from the leaf to other plant

Psrls'

..

pl*t

pa.fts

storage organs-such as roots and of the leaves. fn.y may also be detlcted in prirnary an enlarged stem modified for food storage contains stemS. The potato tuber, which is
strLrch'

b.utilizedasenergysourcetluoughcellularrespiratton'or use of the plant' c. converted into storage.o*po*id,like starch for future the- detection of starch in activity in plants is Hence, one indication of prt"t"rviirrriic in actively photcsynthesizing grncrl organs. The presen." o1starcir may be detected

durnbcane or kutsarita' test tube' test MAT&RIALS: , varip g?tadleave-s of Polyscias, bath, ta'p \'vater, 95% ethyl alcohol' beaker' raok, test tube holder, petri dishes, water

tube

nedicine dropper, iodopotassium iodide soiution'

PROCEDURE:

I l.

UUI rne iear Lq !;Hlraiati' Get a healthy variegated leaf of Poiyscias, ciumbcane or l(utsarlta. and white portions of the white portions from the green ones. Note the shape of the green into a test tube % the leaf. Boil the leaf biades in water for 15 to 20 minutes. Transfer gs % ethyl alcohol. Boil in a water bath until ihe leaves are completely

a petri dish' Use the iodine test decolorized. Rinse the leaf blades in tap urate:.. Piace in for the presence of starch. Note the color changes in the leaf' part of the potato tuber. Stain wi;lt z. prepare a fresh mount using a thin section of the inner thin slice of the potato' iodopotassium iodide; allow the stain to cover only half of the

filled with

uDserve uncel tne mrcroscope. unsmmeo poBio s&lrcn grams are sneil-snapeo wlm uun

concentric lines. Iodine changes in color from golden brown to violet, blue, then blueblack in the presence of starch. Fully stained potato starch grains are blue-black in color.

OBSERVATION AND DISCUSSEGN:

L Draw the green and white leaf tamina and indicate the reaction to iodine test for starch. Draw 3 to 5 cells of unstained potato as seen under the HPO. Draw 8-10 cells of staine:d potato as seen under tPO. Indicate the g:zdual change in color as the starch comes in contact with iodopotassium iodide.

BEsv

yiu tT&

##
oF

" ONLY

&r''\
kI

@uUisn - PREscsrcP oF
srARc+r

- VAq.(ENP t gLl,ck)
Starch test for Variesated

|\)o
leaf

PaR<EN\NG
u

&gScENc-E 6F

. 9rlgg

Unsta.ineC potato cells {F{FG)

Stained potato cells

(LPC' '

Ii. Anslver the following questions:

ry r'r-l'l gr*P ct) white oortions of the leaf lamina after What color change, if any, did you observe in ution? What does this indicate? addition iodopotassiurn iodide

EN

ce of starch?

2.

o\rL\ cH[ \0 stllv /h/6 tsvT Nro h

of

90Lvfl0lt. fwt tt?ws

PARKfN't^jF MErilr

vdr l8scsrcf aF SUfCtf

3"

4"

What color change, if any, did you observe in the grsen portions of the isaf, lasxina aiifi: addition/ of iodopotassium iodide solution? What does this indicate? t/-. THl9 i^lpiC+i-t=S iNG LEAI- TO ftur0sT ecvl OF role of the green pigment in photosyn*resis? n\s GPFqa ?\G t tNT Whal

?\\,{tL N\\\c$

tLt\t\3
AIV

,\i\nt{Es\s,
9&vTt

the starch grains in potato be distrlnguistied from bubbles? THE SIAPCI-I.

ruE

0t!

tS

Sftsn-z What,Knd of plant organ is the potato fuber? Can the pgtato tuber manufacture its food by photosSrnthesis?

Why? tro.

tT- lS LL TA

B.E

n the presence of starch in

pomto tuber.
"TU

Y,

What can you conclude f,rom this exercise? 'T\}E ?SECF-NCE OF sTA ecU

\!) T$e PnpS

Y,
FOU

rooD
8.* S1[-AIN \

vq

UP WqE N tLIPiCATEV OuUTi 6 P ' \O9r$ e

49

Exsr*is* ?4 Liberation of Oxrygeu'in Fhotosynthesis water' A part of the The free oxygen prod.r".d i-o photosynthesis comes e-ntirely from although a' large oxygen that is produced in photosynthesis rnay be used in respiration, aarouat of it is liberated into the envitonment' match' digman MATERIALS: beaker (500 ml or bigger), funnel, test tube, water, splinter'
shoots.

PROCEDUR.E: funnel at the bottorn of a As shown in Figure, place the leaff shoots of digman inside a test tube with water and inve* it 500-m1 beaker about % fiilof water. completeiy fril a be covered with a piece of over the stem of the funnel. (The mo'tl of tne test tube may ,p*f*tou piastic while being invmted i.nto the beaker with water:) See to it that there is no stari or the exercise' fube air space in the test tube at the start of rne exerurss' , i rrr_: until '" ' 2. Expose the setup to iight. observe the bubbles coming from the shoot' wait completely displaces the water in the inverted test tube' tift the test tube carefirlly and immediately insert a owing splinter into the test tube' Observe the giowing sPlinter' OBSERVATTON A.ND DISCUSStrON: lo/ Dravs a:rd label the setup in this V

i.

i.

,i
-t'--'-'-*

'l i

5;f\c

tp

tt
1

Setup to demonstrate the liberation of ory Answer the fotlowing questions: Why was the setuP exPosed tc 1i.ght?
?ROD

ng photosynthesis
)o : 9e 2_aueuA-Eldidtu

'aes,s wry"
ty' erouuwS
is?

ry

ng:s^ 6PoW Jo
0

DGqs'

.,

Wttutlu the effect of light on gas production?

ueniT
TosYlu
.

Lra\rT

r,s

trte iareu'ist \N qAs-139:9c1\9*

gas production and photo N

l-l,tNPf

/'116t

+UP Cz+s

fuoCro,

3.
,ty,

Wh-t i, th" ,-taiionship between

t^ll:nJ.t"pp-**d t" ihe glow.ing splinter when inserted into indicare? rur SALrweB tGN\-TTe \N.J}E* trsr r
WhV is photosynthesis imp+rtant to meur? l\Es\ tg lM ? THL C

rtON \e,

\ oF,

9$o\

THE s \q
rube? What do*s this

J.

rS

'llJE

M)PT

a{vq r!eA-Llq-^t