Luciferase PCR Template

encoding Luciferase.

in vitro transcription template for large-scale synthesis of mRNA

he Stealth Express Luciferase PCR template for In vitro transcription allows for the large-scale synthesis of mRNA encoding Luciferase gene. With the mRNA product, exogenous Luc expression can be achieved when transfected into target cells. This new gene delivery technology is faster and more efficient than conventional virus-based approaches. 10 g of template is provide, enough to make hundreds of micrograms of Luciferase encoding mRNA. Within each template, the coding sequence for a protein is flanked by 5 and 3 untranslated regions designed to maximize the translational efficiency and cytoplasmic stability of the mRNA. The template drives incorporation of 120-nt polyA tails into RNA transcripts, eliminating the need to enzymatically polyadenylate IVT products before use.

Box 1 | Product Info

Content Qty

Features
Template contains the T7 phage polymerase promoter allowing the widely available T7 polymerase to be used. Inherently non-mutagenic PCR template for footprint-free gene expression Reliable expression levels

Cat#: ABP-RP-SELUC Luciferase linear DNA template ready to use directly in IVT reactions. 10 g

Storage -20C

or Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Allele Biotech is strictly prohibited

Eliminates safety concerns and bio-containment issues associated with virus

Box 2 | Components Needed for IVT

Component
Modified UTP

Cat. No.
ABP-PP-NTPMODU

Description
rNTPs for In vitro transcription. Modified Uracil is required to ameliorate immunological responses towards the mRNA product. rNTPs for In vitro transcription. Modified Cytosine is required to ameliorate immunological responses towards the mRNA product. rNTPs for in vitro transcription. rNTPs for in vitro transcription. RNA polymerase for In vitro Transcription

Modified CTP

ABP-PP-NTPMODC

Standard ATP Standard GTP T7 polymerase

ABP-PP-NTPSTDA ABP-PP-NTPSTDG ABP-PP-T7POL

ARCA

ABP-PP-NTPARCA

Anti-Reverse Cap Analog; transcripts produced with ARCA have increased translation in comparison with conventional cap analogs.

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